in vitro fertilization embryo transfer
Post on 25-Dec-2014
1.112 Views
Preview:
DESCRIPTION
TRANSCRIPT
IN VITRO FERTILIZATION-EMBRYO TRANSFER (IVF-ET)
Presented by
NIYAMAT M. PANJESHA
A seminar on
Modern College Of Arts , Science & Commerce.Shivajinagar, Pune-05.
WHAT IS IVF?
In vitro fertilization broadly deals with “the removal of egg’s from a women, fertilizing them in the laboratory, and transferring the fertilized eggs (zygotes) into the uterus a few days later.
WHY IVF?
Infertility: Extremely Prevalent Definition: inability to achieve pregnancy 17-26% of couples worldwide (90 million
women) 27-47% of infertile couples have impaired
male fertility; decline in semen quality worldwide over last 50 years
WHY THE INCREASE IN INFERTILITY?
Delayed marriage and child-bearing Sexually transmitted diseases Hormonal contraception Abortion Obesity Environmental Pollution
Male infertility Female infertility
IVF: HISTORY
In vitro fertilization (IVF) is a fertility procedure which first succeeded as recently as 1978 by Dr. Edwards ( an embryologist) and Dr. Steptoe ( a gynecologist) in England. Since then the technology has been further refined and developed by physicians and embryologists, with over 20,000 babies born worldwide.
METHODOLOGY OF IVF
The ivf broadly involves the fowllowig stages:-
1. Ovarian stimulation and monitoring2. Egg (oocyte) retrieval3. Fertilization4. Intracytoplasmic Sperm Injection5. Embryo transfer
STAGE 1: OVARIAN STIMULATION ANDMONITORING
Ovarian stimulation is used to produce multiple mature follicles, rather than the single egg normally developed each month.
STAGE 2: EGG RETRIEVAL
Egg recovery is accomplished by a transvaginal procedure, which can be performed under light sedation or local anesthesia.
NORMAL EGG ABNORMAL EGG
STAGE 3: FERTILIZATION
Once mature eggs have been retrieved, the sperm and eggs are placed together in the laboratory and incubated in a meticulously prepared culture medium at a temperature identical to that of the woman’s body.
STAGE 4: INTRACYTOPLASMIC SPERMINJECTION
During the process of ICSI, an embryologist isolates a sperm cell, draws it up into a microscopic needle and injects it inside an egg using a high power microscope.
STAGE 5: EMBRYO TRANSFER
Embryo transfer is not a painful procedure and can be performed without sedation.
Usually up to three embryos are placed together in a catheter ( a tubular instrument) and transferred, via the vagina, into the uterus.
SUCCESS RATE OF IVF
Varies from programme to programme. It is dependent upon correct diagnosis
of patient, and age. Pregnancy rate is in the range of 25-
30% per oocyte retrieval. The take home is about 15-20% per
procedure.
FACTORS
Increased risk of abortion. Multiple pregnancy. Ectopic pregnancy. Low birth weigh baby. Premature delivery.
LIMITATION OF IVF
Genetic defect in oocytes. Genetic defect in fertilizing sperm. Environmental mutagenesis. Inadequate supply of nutrients and
hormones. Exposure to toxic agents and free
radicals.
WORLDS FIRST TEST TUBE BABY
REFERENCES
ANY QUESTION
?
THANK YOU
top related