intro to labs 1-4 labs n stuff. slide 2 of 20 lab #1 – diffusion & osmosis 4 parts 1....

Intro to Labs 1-4

Labs ‘n stuff

Slide 2 of 20

Lab #1 – Diffusion & Osmosis

4 Parts1. Diffusion – Qualitative2. Osmosis – Quantitative & Graph3. Molar Concentration – Quantitative, Graph, and Inference4. Water Potential – Calculation4b. Plasmolysis – if you have not scared or scarred me.

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Lab #1 – Diffusion & Osmosis

Should get good results

Simple

Understand what you are doing, don’t just follow the steps!!

Think of this lab as intro to AP Labs

Slide 4 of 20

Lab #1 – Part 1

You will put two substances in a bag made of dialysis tubing.

You will determine whether they exit/enter the dialysis tubing bag. To do this, you will use either indicator

strips or IKI (potassium Iodide or Lugol’s solution)

Data (color) will be recorded in a table

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Lab #1 – Part 2

Fill dialysis bags with different concentrations of sucrose solution, place bags in beaker with water. 0.0 M 0.2 M 0.4 M 0.6 M 0.8 M 1.0 M

Weigh each (dialysis bag + sucrose solution) BEFORE and AFTER to calculate Percent Change in Mass

Make line graph

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Lab#1 – Part 2

Why do we use sucrose solution?

What would change if we used a salt solution?

Which bags will increase in mass?

Which bags will decrease in mass?

What is responsible for the increase/decrease in mass?

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Lab #1 – Part 3

Pieces of potato (or other vegetable) placed in various sucrose solutions

What sucrose concentrations we will use?

Measure mass of potato before being placed in solutions to calculate Percent Change in Mass

Must be left Overnight.

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Lab#1 – Part 3

Why do we use sucrose solution?

Which potato samples will increase in mass?

Which potato samples will decrease in mass?

What is responsible for the increase/decrease in mass?

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Lab #1 – Part 4

Calculate Water Potential

Water potential = Solute potential + Pressure potential

Ψ = Ψs + Ψp

Ψs = -iCRT i = 1 C = Molar Concentration R = 0.0831 (liter*bar)/(mole*K) T = temperature in Kelvin

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What to do next?

Organize yourselves into groups of 3-4

Each group is responsible for getting the following: 12 clear or translucent plastic cups (10oz or more) Sharpie Permanent Marker Label Cups

Name of group + Period 2 sets of (0.0M, 0.2M, 0.4M, 0.6M 0.8M 1.0M)

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When?

Friday 12/2 Lab #2 Activity (Toothpickase) Lab #3 Virtual Lab

Monday 12/5 Start Lab #1 & Pre-Lab Quiz (12/5)

Tuesday 12/6 Finish Lab #1 Start Lab #4 Pre-Lab Quiz – Lab #4

TEST: Labs 1-4 on Monday (12/12)

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Lab #2 – Enzyme Kinetics

We will do a modified version of Lab #2

Toothpickase

Self-Contained Lab Exercise

Separate yourself into groups of 3-4

Need: Approximately 600 flat toothpicks Approximately 100 round or reinforced toothpicks

Only need to be harder to break than the flat toothpicks

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Lab #3 – Mitosis & Crossing Over

Virtual Lab Exercise

Data Group Assignment (1-8) by teacher.

First, identify cells in various stages of Mitosis (including interphase)

Second, calculate map units and draw conclusions

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Lab #4 - Photosynthesis

2nd Actual Lab

2 Parts Chromatography (Semi-FAIL) Part that fails miserably

Fingers Crossed?

IF worse comes to worse, you will just analyze given data

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Lab #4 – Part 1

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Lab #4 – Calculate Rf values

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Chromatography

http://www.phschool.com/science/biology_place/labbench/lab4/quiz1.html

Use Online Animations:

http://www.phschool.com/science/biology_place/labbench/lab4/concepts2.html

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Part that goes well

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Part that does not go well

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Results Analysis

http://www.phschool.com/science/biology_place/labbench/lab4/quiz2.html