introduction to fluorescence (two-photon, three steps, phase incoherent process)
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INTRODUCTION TO FLUORESCENCE(two-photon, three steps, phase incoherent process)
•Einstein’s coefficients, dipole transitions.• Experimental setup•T-configuration•Jablonsky diagram•Franck-Condon principle, reflection rule and Stokes shift•Lifetime and quantum yield•Fluorescence quenching (collisional) •London’s theory of molecular forces
•RET resonant Energy Transfer•FRAP (Fluorescence Recovery after Photo Bleaching
Quantum yield Q and lifetime τ
Γ=spectral emissivity, knr rate of non radiative transitions
teoricamente
Center for fluorescence spectroscopy (J. Lakowicz) http://cfs.umbi.umd.edu/ISS http://www.iss.com/Fluorescence foundation http://www.fluorescence-foundation.org/Enrico Gratton’s homepage http://www.lfd.uci.edu/~gratton/Laboratory of fluorescence dynamicsGregorio Weber http://www.lfd.uci.edu/weber/
CORSO DI MICROSCOPIA OTTICA DI CLEGGhttp://online.physics.uiuc.edu/courses/phys598om/spring12/
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