lab meeting 25/9

Detecting Viral Infections Using

MicroarraysNacho Caballero

Connor LabWednesday, September 26, 12

RIG-I

Lassa

Wednesday, September 26, 12

RIG-I

Lassa

uninfected

Wednesday, September 26, 12

RIG-I

Lassa

uninfected

1.30

-0.74

Wednesday, September 26, 12

RIG-I

Lassa

uninfected

3 dpi

1.10

2.83

1.30

-0.74

Wednesday, September 26, 12

RIG-I

Lassa

uninfected

3 dpi

1.10

2.83

1.30

-0.74

Marburg

3 dpi

uninfected

4.72

3.24 4.30

2.37

Wednesday, September 26, 12

Wednesday, September 26, 12

uninfected-2 dpi 3 dpi-12 dpi

Wednesday, September 26, 12

uninfected-2 dpi 3 dpi-12 dpi

LassaMarburg

Wednesday, September 26, 12

uninfected-2 dpi 3 dpi-12 dpi

TP34

TN38

FP0

FN0

LassaMarburg

Wednesday, September 26, 12

uninfected-2 dpi 3 dpi-12 dpi

TP34

TN38

FP0

FN0

LassaMarburg

Wednesday, September 26, 12

uninfected-2 dpi 3 dpi-12 dpi

TP34

TN38

FP0

FN0

LassaMarburg

Sensitivity: 1 Specificity: 1

Wednesday, September 26, 12

uninfected-2 dpi 3 dpi-12 dpi

LassaLassa Marburg MarburgTP34

TN53

FP0

FN33

Sensitivity: 0.5 Specificity: 1

LassaMarburgBlind

Wednesday, September 26, 12

uninfected-2 dpi 3 dpi-12 dpi

LassaLassa Marburg MarburgTP38

TN29

FP4

FN6

Sensitivity: 0.86 Specificity: 0.88

LassaMarburgBlind

BlindMarburg

Wednesday, September 26, 12

Next Steps

Wednesday, September 26, 12

Next Steps

Use Agilent v2 microarraysto distinguish between Lassa and Marburg

Wednesday, September 26, 12

Next Steps

Use Agilent v2 microarraysto distinguish between Lassa and Marburg

Use RNA-Seqto identify non-coding RNAsand obtain better resolution of transcriptome

Wednesday, September 26, 12