lecture 4

Endocrine Physiologylecture 4

Dale Buchanan Hales, PhD

Department of Physiology & Biophysics

Antidiuretic hormone and the mineralcorticoids

Synthesis of ADH

• It is synthesized as pre-prohormone and processed into a nonapeptide (nine amino acids). • Six of the amino acids form a ring structure, joined by

disulfide bonds. • It is very similar in structure to oxytocin, differing only in

amino acid #3 and #8.

• ADH synthesized in the cell bodies of hypothalamic neurons in the supraoptic nucleus

• ADH is stored in the neurohypophysis (posterior pituitary)—forms the most readily released ADH pool

Hypothalamus and posterior pituitary

Structure of ADH

• Mechanical disruption or the neurohypohyseal tract by trauma, tumor, or surgery temporarily causes ADH deficiency.

• ADH will be restored after regeneration of the axons (about 2 weeks).

• But if disruption happens at a high enough level, the cell bodies die in the hypothalamus resulting in permanent ADH deficiency

Synthesis of ADH

Antidiuretic Hormone: ADH

• ADH is also known as arginine vasopressin (AVP = ADH) because of its vasopressive activity, but its major effect is on the kidney in preventing water loss.

ADH: conserve body water and regulate tonicity of body fluids

Regulated by osmotic and volume stimuli

Water deprivation increases osmolality of plasma which activates hypothalmic osmoreceptors to stimulate ADH release

ADH increases renal tubular absorption of water

Primary action of ADH: antidiuresis

• ADH binds to V2 receptors on the peritubular (serosal) surface of cells of the distal convoluted tubules and medullary collecting ducts.

• Via adenylate cyclase/cAMP induces production and insertion of AQUAPORIN into the luminal membrane and enhances permeability of cell to water.

• Increased membrane permeability to water permits back diffusion of solute-free water, resulting in increased urine osmolality (concentrates urine).

ADH: conserve body water and regulate tonicity of body fluids

Regulated by osmotic and volume stimuli

Water deprivation increases osmolality of plasma which activates hypothalmic osmoreceptors to stimulate ADH release

Secretion of ADH

• The biological action of ADH is to conserve body water and regulate tonicity of body fluids.

• It is primarily regulated by osmotic and volume stimuli.

• Water deprivation increases osmolality of plasma which activates hypothalmic osmoreceptors to stimulate ADH release.

• Conversely, water ingestion suppresses osmoreceptor firing and consequently shuts off ADH release.

• ADH is initially suppressed by reflex neural stimulation shortly after water is swallowed.

• Plasma ADH then declines further after water is absorbed and osmolality falls

Secretion of ADH

Pathway by which ADH secretion is

lowered and water excretion raised

when excess water is ingested

• If plasma osmolality is directly increased by administration of solutes, only those solutes that do not freely or rapidly penetrate cell membranes, such as sodium, cause ADH release.

• Conversely, substances that enter cells rapidly, such as urea, do not change osmotic equilibrium and thus do not stimulate ADH release.

• ADH secretion is exquisitely sensitive to changes in osmolality.

• Changes of 1-2% result in increased ADH secretion.

Secretion of ADH– osmolality control

ADH and plasma

osmolality

• ADH is stimulated by a decrease in blood volume, cardiac output, or blood pressure.

• Hemorrhage is a potent stimulus of ADH release. • Activities, which reduce blood pressure, increase

ADH secretion. • Conversely, activities or agents that increase blood

pressure, suppresses ADH secretion.

Secretion of ADH—hemodynamic control

ADH and blood

pressure

Pathway by which ADH secretion and tubular

permeability to water is increased when plasma

volume decreases

• Hypovolemia is perceived by “pressure receptors” -- carotid and aortic baroreceptors, and stretch receptors in left atrium and pulmonary veins.

• Normally, pressure receptors tonically inhibit ADH release.

• Decrease in blood pressure induces ADH secretion by reducing input from pressure receptors.

• The reduced neural input to baroreceptors relieves the source of tonic inhibition on hypothalamic cells that secrete ADH.

• Sensitivity to baroreceptors is less than osmoreceptors– senses 5 to 10% change in volume

Secretion of ADH

Hypothalamus, posterior pituitary and ADH secretion– connection with baroreceptors

• Hypovolemia also stimulates the generation of renin and angiotensin directly within the brain.

• This local angiotensin II enhances ADH release in addition to stimulating thirst.

• Volume regulation is also reinforced by atrial naturetic peptide (ANP).

• When circulating volume is increased, ANP is released by cardiac myocytes, this ANP along with the ANP produced locally in the brain, acts to inhibit ADH release.

Secretion of ADH

• The two major stimuli of ADH secretion interact.

• Changes in volume reinforce osmolar changes.

• Hypovolemia sensitizes the ADH response to hyperosmolarity.

Secretion of ADH

Plasma Osmolality vs. ADH

The set point of the system is defined as the plasma osmolality value at which ADH secretion begins to increase. Above this point slope is steep reflecting sensitivity of system. Set point varies from 280 to 290 mOsm/kg H2O

Blood volume vs. ADH

When blood volume or arterial pressure decreases, inhibitory input from baroreceptors is over ridden and ADH secretion is stimulated. Normally, signals from baroreceptors tonically inhibit ADH secretion.

Interaction between osmolar and blood volume/pressure stimuli

With a decrease in blood volume, set point shifts to lower osmolality and slope is steeper. During circulatory collapse kidney continues to conserve water despite reduction in osmolality. With increase in blood volume, set point shifts to higher point and sensitivity is decreased.

Actions of ADH

• The major action of ADH is on renal cells that are responsible for reabsorbing free (osmotically unencumbered) water from the glomerular filtrate.

• ADH responsive cells line the distal convoluted tubules and collecting ducts of the renal medulla.

• ADH increases the permeability of these cells to water.

• The increase in membrane permeability to water permits back diffusion of water along an osmotic gradient.

• ADH significantly reduces free-water clearance by the kidney

Actions of ADH

• ADH action in the kidney is mediated by its binding to V2 receptors, coupled to adenylate cyclase and cAMP production.

• cAMP activates protein kinase A which prompts the insertion of water channels into the apical membrane of the cell.

• When ADH is removed, the water channels withdraw from the membrane and the apical surface of the cell becomes impermeable to water once again. .

Actions of ADH

• This mechanism of shuttling water channels into and out of the apical membrane provides a very rapid means to control water permeability

• The basolateral membrane of the ductal cells are freely permeable to water, so any water that enters via the apical membrane exits the cell across the basolateral membrane, resulting in the net absorption of water from the tubule lumen into the peritubular blood.

• Water deprivation stimulates ADH secretion, decreases free-water clearance, and enhances water conservation.

• ADH and water form a negative feedback loop.

Actions of ADH

Inputs reflexly controlling thirst.

• ADH deficiency is caused by destruction or dysfunction of the supraoptic and parventricular nuclei of the hypothalamus. Inability to produce concentrated urine is a hallmark of ADH deficiency and is referred to as diabetes insipidus.

• ADH also acts on the anterior pituitary to stimulate the secretion of ACTH.

Actions of ADH

Aldosterone and the mineralocorticoids

• The mineralocorticoid, aldosterone is vital to maintaining sodium and potassium balance and extracellular fluid volume.

• Aldosterone is an adrenal corticosteroid, synthesized and secreted by the adrenal cortex.

Cross section through the adrenal gland– cortex and medulla

salt

sugar

sex

Aldosterone

• The adrenal cortex is composed of three major zones, differentiated by the histological appearance and type of corticosteroid they produce.

• The outermost is the zona glomerulosa, is very thin and consists of small cells with elongated mitochondria.

• The middle zona fasiculata is the widest zone and consists of columnar cells that are highly vacuolated with numerous lipid droplets.

• These lipid droplets are composed of cholesterol esters the substrate for adrenal steroid hormone biosynthesis.

Adrenal zones

• The innermost zona reticularis contains fewer lipid droplets than fasiculata cells, but have similar mitochondria.

• ACTH has trophic effects on the zona fasiculata and reticularis.

Adrenal zones

• Aldosterone is synthesized and secreted by the zona glomerulosa .

• The synthesis of aldosterone from cholesterol to corticosterone is identical to the synthesis of glucocorticoids in the zona fasiculata.

• The C18 methyl group of corticosterone is hydroxylated and converted to an aldehyde yielding aldosterone.

Aldosterone synthesis

Aldosterone synthesis

• ACTH also stimulates aldosterone synthesis.• However the ACTH stimulation is more transient than

the other stimuli and is diminished within several days. • ACTH provides a tonic control of aldosterone

synthesis. • In the absence of ACTH, sodium depletion still

activates renin-angiotensin system to stimulate aldosterone synthesis.

• Aldosterone levels fluctuate diurnally—highest concentration being at 8 AM, lowest at 11 PM, in parallel to cortisol rhythms.

Aldosterone synthesis in the adrenal

zona glomerulosa

• The principal function of aldosterone is to sustain extracellular fluid volume by conserving body sodium.

• Aldosterone is largely secreted in response to signals that arise from the kidney when a reduction in circulating fluid volume is sensed.

• When body sodium is depleted, the fall in extracellular fluid and plasma volume decreases renal arterial blood flow and pressure.

Aldosterone function

Aldosterone action

• Aldosterone binds to the mineralocorticoid receptor in target cells and affects transcriptional changes typical of steroid hormone action.

• The kidney is the major site of mineralocorticoid activity.

• Increased blood pressure results from excess aldosterone.

• Hypertension is an indirect consequence of sodium retention and expansion of extracellular fluid volume.

Aldosterone action

Regulation of aldosterone secretion: Activation of renin-angiotensin system in response to hypovolemia is predominant stimulus for aldosterone synthesis.

Components of renin-

angiotensin-aldosterone

system

• The juxtaglomerular cells of the kidney respond to hypovolemia by secreting renin. Renin acts on angiotensinogen (which is secreted by the liver) to form angiotensin I which is further cleaved by angiotensin converting enzyme (which is secreted by the lungs) to angiotensin II.

Aldosterone and renin-AII

• Angiotensin II acts on the zona glomerulosa to stimulate aldosterone synthesis.

• Angiotensin II acts via increased intracellular cAMP to stimulate aldosterone synthesis.

Aldosterone

• ANP reinforces the effects of the renin-angiotensin system on aldosterone secretion.

• In response to volume expansion, artrial myocytes secrete ANP which binds to receptors in the zona glomerulosa to inhibit aldosterone synthesis.

• ANP acts via increased intracellular cGMP which opposes cAMP and inhibits aldosterone synthesis.

• ANP also reduces aldosterone indirectly by inhibiting renin release.

Aldosterone

Action of aldosterone on the renal tubule. Sodium reabsorption from tubular urine into the tubular cells is stimulated. At the same time, potassium secretion from the tubular cell into urine is increased. Na+/K+-ATPase, and Na+ channels work together to increase volume and pressure, and decrease K+.

Aldosterone mechanism

• The aldosterone-induced proteins serum and glucocorticoid-inducible kinase (Sgk), corticosteroid hormone-induced factor (CHIF), and Kirsten Ras (Ki-Ras) increase the activity and/or no. of these transport proteins during the early phase of action

Aldosterone action

• Aldosterone stimulates the active reabsorption of sodium from the tubular urine back into the nearby capillaries in the distal tubule.

• Water is passively reabsorbed with sodium which maintains sodium concentrations at a constant level.

• Hence extracellular fluid volume expands in a virtually isotonic fashion

Pathway by which aldosterone secretion and tubular sodium

reabsorption is increased when plasma

volume is decreased

Aldosterone clears potassium

• Aldosterone facilitates the clearance of potassium from the extracellular fluid, and potassium stimulates aldosterone synthesis—thus providing a feedback control mechanism to control potassium levels.

• Conversely, potassium depletion lowers aldosterone secretion.

• Potassium stimulates aldosterone synthesis by depolarizing zona glomerulosa cell membranes to stimulate aldosterone synthesis.

• Aldosterone stimulates the active secretion of potassium from the tubular cell into the urine.

• Most potassium that is excreted daily results from distal tubular secretion.

• Hence aldosterone is critical for disposal of daily dietary potassium load at normal plasma potassium concentrations.

Aldosterone action

Pathway by which an increased

potassium intake induces greater

potassium excretion mediated by aldosterone

Summary of aldosterone

system

Aldosterone time course of action

Aldosterone genomic vs. non-genomic

Aldosterone Action

Cortisol is at 1000 fold higher concentrations than aldosterone

• Cortisol binds well to the mineralocorticoid receptor and plasma cortisol levels are orders of magnitude higher than aldosterone.

• Target tissues for aldosterone are protected from glucocorticoid excess via the action of 11-hydroxysteroid dehydrogenase, the enzyme that converts cortisol to cortisone, a biological inactive metabolite.

• Aldosterone is not a substrate for 11-HSD and thus only it can bind to its receptor.

Aldosterone action

Integrated control of water and sodium homeostasis

• All of the renal, adrenal, cardiac, vascular, brain and endocrine influences on body fluid homeostasis converge on kidney as final site of regulation– AII and aldosterone are anti-natiuretic– Increase Na+, result in water retention, increase plasma

volume and perfusion pressure

• Counter regulator effects of ANP– Stimulate urinary Na+ and water excretion– Inhibit antidiuretic effects of ADH

• Imbalances in any one of these hormones affects volume status and plasma osmolar state

Integrated control of water and sodium homeostasis

Hyperaldosteronism

• Hyperaldosteronism is known to be caused by primary overproduction of aldosterone in conditions such as Conn’s syndrome.

• Conditions of low cardiac output are also known to stimulate synthesis of aldosterone.

• Both conditions result in sustained hypertension.

Hyperaldosteronism

• Previously, the hypertension associated with hyperaldoseteronism was thought to be mediated exclusively through the mineralocorticoid receptor located in “classical” epithelial target tissues and result from renal sodium retention.

• We now know that cardiac hypertension associated with hyperaldosteronism is far more complex.

• The treatment of patients with severe congestive heart failure with spironolactone (mineralocorticoid antagonist) produced significant reduction in mortality and morbidity, despite the very modest diuretic effect of the drug.

• The demonstration of local synthesis of aldosterone by cardiac and vascular cells

• The demonstration of high affinity, low capacity binding proteins for mineralocorticoids in cardiac myocytes and vascular endothelial cells.

Hyperaldosteronism

Hyperaldosteronism

• The demonstration that 11-beta-hydroxysteroid dehydrogenase is expressed in cardiac myocytes.

• The identification of classic mineralocorticoid receptor in paraventricular nuclei and amygdala in the brain, regions known to be associated with salt intake.