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LIMOR MAN M.D, M.MED.SC

ASSISTANT PROFESSOR OF RESEARCH IN OBSTETRICS AND GYNECOLOGY

RONALD O. PERELMAN AND CLAUDIA COHEN CENTER FOR REPRODUCTIVE MEDICINE

WEILL CORNELL MEDICINE, NYC, NY, USA

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• MINIMIZE ISCHEMIC INJURY TO THE

TRANSPLANT BY REDUCING THE LATENCY

PERIOD BEFORE NEOVASCULARIZATION

IMPROVE SURVIVAL

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-Baird DT, Campbell B, de Souza C, Telfer E. Long-term ovarian function in sheep after ovariectomy and autotransplantation of cryopreserved cortical strips. Eur J Obstet Gynecol Reprod Biol. 2004 Apr 5;113 Suppl 1:S55-9.

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-Dolmans MM, Martinez-Madrid B, Gadisseux E, Guiot Y, Yuan WY, Torre A, Camboni A, Van Langendonckt A, Donnez J. Short-term transplantation of isolated human ovarian follicles and

cortical tissue into nude mice. Reproduction. 2007 Aug;134(2):253-62.

0

20

40

60

80

100

InVIVO Postgra ing

%Primordialfollicles

primordial primary preantral antral

AMH AMH

FSH FSH

Metabolic demands

-Gougeon et al. Human Reproduction 1986

In vitro

activation

protocols

? Controlled

Ovarian

Hyperstimulation

(COH)

-Kawamura et al. PNAS. 2013.

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ROBUST ENRICHMENT OF IGF-1 IN CULTURE

ng

/mL

P<0.001

Vectors-IGF-1

Human Endothelial Cells

0

200

400

600

800

1000

1200

1400

1600

ECs IGF-ECs

IGF-1

EXPERIMENTAL DESIGN

Human Ovarian Tissue

Fibrin clot

CONTROL GROUP STUDY GROUP

END POINT 3 WEEKS n=8 n=6

0

20

40

60

80

PrimordialPrimarySecondary

Blank

IGF

P=0.41 P=0.03 P=0.05

%

0

5

10

15

20

25

30

SecSimple Secpre-antral

Blank

IGF

P=0.22 P<0.001

%

CELL-BASED STRATEGY

-Gougeon et al. Human Reproduction 1986

In Vitro

Activation

Protocols

IGF-1

Co-

Transplantation COH

Daylon James

Laura Park

Jovana Lekovich

Glenn Schattman

Zev Rosenwaks

Nikica Zaninovic

Richard Bodine

Michael Ginsberg