microglial marker labeled iba1 antibody · labeled iba1 antibody iba1 is a protein highly expressed...
Post on 27-Sep-2018
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Labeled Iba1 AntibodyIba1 is a protein highly expressed in microglia and macrophage with a molecular weight of about 16.7kDa The protein is a commonly known microglial marker in the nervous system. These items are Anti Iba1, Rabbit (for Immunocytochemistry): Code No. 019-19741, labeled with Biotin or Red Fluorochrome like Cy5TM
Assay ConditionSample
Cerebral cortex of 7-week Wistar rat or 7-week ICR mouseSection
50μm- frozen section Antibody concentration 1/200
Anti Iba1, Rabbit Code No.
019-19741
Secondary antibody
biotin-conjugated
Anti Iba1, Rabbit, Biotin-conjugated
Rat Mouse
Anti Iba1, Rabbit, Red
Fluorochrome(635)-conjugate
Assay ConditionSample
Cerebral cortex of 7-week Wistar rat or 7-week ICR mouseSection
50μm- frozen section Antibody concentration 1/200
The secondary antibody process could be eliminated
Evaluate by immunohistochemistry test
Reduced background
Applications –Immunohistostaining-
Rat Mouse
Anti Iba1, Rabbit, Biotin-conjugated Anti Iba1, Rabbit, Red Fluorochrome(635)-conjugated
Features
The item shows clearer localization signals of microglia than using secondary antibody.
All immunohistochemistry data was provided by Sanagi ,T. , Ichinohe ,N., and Kohsaka, S., National Center of Neurology and Psychiatry in Japan.
Anti Iba1, Rabbit Code No.
019-19741
Secondary antibody Alexa Fluor® 647
-conjugated
The item shows the localization signals of microglia as well as using secondary antibody.
Microglial marker
Anti Iba1, Rabbit, Biotin-conjugated
Anti Iba1, Rabbit, Red Fluorochrome(635)-conjugated
Conjugate Biotin Red Fluorochrome (Excitation=634nm, Emission=654nm)
Antigen Synthetic peptide corresponding to C-terminus of Iba1
Buffer PBS with 0.05% Sodium Azide
Species cross reactivity Mouse, Rat, and Marmoset Mouse and Rat
Application Immunohistochemistry 1:200-2,000
Antibody information
Product list
Product Name Wako Cat. No Pkg. Size Grade Storage Condition
Anti Iba1, Rabbit (for Immunocytochemistry) 019-19741 50 g For Immunochemistry Keep at -20 .
Anti Iba1, Rabbit (for Western Blotting) 016-20001 50 g For Immunochemistry Keep at -20
Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64) 015-25191 50μL For
Immunochemistry Keep at -20
Anti Phosphorylated α-Synuclein, Monoclonal Antibody(pSyn#64), Biotin-conjugated 010-26481 100μL For
Immunochemistry Keep at 2-10 .
Anti Phosphorylated α-Synuclein, Monoclonal Antibody(pSyn#64), FITC-conjugated 017-26491 100μL For
Immunochemistry Keep at 2-10 .
Related Products
Product Name Wako Cat. No Pkg. Size Grade Storage Condition
Anti Iba1, Rabbit, Biotin-conjugated 016-26461 100 For Immunochemistry Keep at 2-10 .
Anti Iba1, Rabbit, Red Fluorochrome(635)-conjugated 013-26471 100 For Immunochemistry Keep at 2-10 .
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