mock proposal s16697

Allelopathy Effect of Leonorus sibiricus Extract on Turmeric and Its Microbial

Activity on Selected Disease

PresenterSyed khairul syahmi bin syed ahmad

S 16697BACHELOR SCIENCE OF BIOINDUSTRY

SupervisorASSOC. PROF. DR. NUR ASHIKIN PSYQUAY BINTI

ABDULLAH

OUTLINE

Introduction Literature review Problem statement Objective Material and methodology Analysis Data Planning Expected outcome References

INTRODUCTION

Leonorus sibiricus (motherwort)

Herb natively found in India. Acknowledge as Kacang Ma by the Chinese. Popular among Sarawakians for its medicinal

value and as culinary herbs. Contains terpenoids and phenolics substances

with demonstrated allelopathy effect (Larcher, 2000)

LITERATURE REVIEW

Allelophaty effects

Allelopathy effect is the reaction that are received by plants surrounded them in which either it stimulate the growth or retain the growth performance of the plant.

Different concentration of extract from Leonorus sibiricus from root exudates shows stimulatory effect on rice, wheat and mustard.

(Raktodrone, 2001)

LITERATURE REVIEW cont…

Tissue culture system

Tissue culture also known as micropropagation, which is a practice used to propagate the plant under sterile condition.

(George, 1993).

Plant tissue culture relies on fact that the plant cells have the ability to regenerate a whole plant and this ability is called as totipotency.

(George and Sherington, 1984).

LITERATURE REVIEW cont…

In viro turmeric

The only method open for turmeric improvement is selection of desirable genotypes, based on higher yield, higher curcumin content, disease and insect-pest resistance, and their multiplication and use as planting stocks.

(Shirgurkar et al. 1999)

PROBLEM STATEMENT

Turmeric flowering is rare; the breeding is difficult and the seed does not occur.

It cannot be propagate exclusively by rhizome.

Optimal plant growth regulator concentration for in vitro of turmeric is differs according to previous journal papers.

OBJECTIVE

To determine the optimum L. sirbircus extract for better growth performances of turmeric.

To determine the effect of L. sirbicus in contamination.

MATERIALSAND

METHODS

PLANT ANALYSIS

In vitro Propagation

In vitro Propagation

The experiment will be conducted with 5 different

concentrations of Leonorus sibiricus concentration arranged in Completely Randomized Design (CRD) with 10 replications.

Duncan Multiple Test Ranges will be used to compare the least significance difference between at p= 0.05 using SAS programme.

DATA ANALYSIS

Activity/ month

Sept Oct Nov Dec Jan Feb March April May June

Sample collection

Lab analysis

Data analysis

Writing thesis

PLANNING

1. Optimum concentration of Leonorus sibiricus extract for a better growth performance of turmeric will be known.

2. Effect of Leonorus sibiricus towards the in-vitro contamination will be known.

3. Identification on performance of Leonorus sibiricus in preventing contamination from bacteria and fungus.

EXPECTED OUTCOME

References

Thank You