molecular beacon fluorescence detection of pseudogene shmt-psi using two molecular beacon probes,...
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Molecular beacon fluorescence detection of pseudogene SHMT-psI using two molecular beacon probes, E8/E10 and E9/E10
Cladding
Core
Exciting Laser
Fluorescence Generated at Surface
Fluorescence coupled into fiber.
Fluorophore labeled Phospho-STAT3 antibodies.
Phospho-STAT3
Phospho-STAT3 antibodies (IgG)
400 450 500 550 600 650 7000
300
600
900
1200
1500
Flu
ore
sce
nce
inte
nsi
ty in
a.u
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Wavelength in nm
Evanascent wave excited Alexa-430 Spectra in two different probes.
Signal from stimulated Cells Signal from non-stimulated Cells
Detection of Phosphorylated STAT3 Protein by Fiber-Based Sensor
STAT - Signal Transducer and Activator of Transcription protein
Surface plasmon resonance curves. The left-hand side curve is for just the silver film (labeled Ag); the right-hand side shows the curve (labeled Ag/p-4-BCMU) shifted on the deposition of a monolayer Langmuir-Blodgett film of poly-4-BCMU on the silver film
Close packing of colloidal nanospheres to form a photonic crystal of close-packed colloidal array. (Left) Atomic Force Microscope (AFM) image of the surface layer. (Right) Scanning Electron Microscope (SEM) image of a cross-section (Markowicz and Prasad; unpublished).
Photonic Crystal Sensors
Visible extinction spectra showing how diffraction depends on the glucose concentration for a 125µm-thick PCCA glucose sensor. The ordinate is given as –log T, where T is the transmittance. The PCCA expands for concentrations between 0.1 and 0.5 mM glucose.
ACIS, November 2003
Basic concepts:- Use molecularly specific fluorescent tags to label cells
- Single-file flow of cells (hydrodynamic focusing)
- Laser illumination of single cells
- Multiparameter fluorescence detection
-Data analyses and/or sorting
FLOW CYTOMETRY
Applications
• HIV Monitoring• Leukemia or Lymphoma Immunophenotyping • Organ Transplant Monitoring• DNA Analysis for Tumor Ploidy and SPF• Primary and Secondary Immunodeficiency• Bacterial contamination in food and water
Biophotonics Opportunities
• Modeling of light propagation in tissue• In-vivo Bioimaging, Spectroscopy, and Optical Biopsy• Real-Time monitoring of Drug Interactions • Optical Biopsy• Nano-Biophotonic Probes• Multiphoton Processes for Biotechnology• Femtolaser based biotechnology\• Nanoarray multianalytic sensors• Multiparameter Molecular Flowcytometry• Single Molecule Biofunctions• Nanomedicine
Acknowledgements
Researchers at the Institute:Prof. E. BergeyProf. A. CartwrightProf. M. SwihartProf. E. FurlaniDr. A. KachynskiDr. A. KuzminDr. Y. SahooDr. H. PudavarDr. T. OhulchanskyyDr. D. BharaliDr. D. LuceyDr. K. BabaDr. J. Liu
DURINT/AFSOR Dr. Charles Lee
Outside CollaboratorsProf. R.BoydProf. J.HausProf. J M J FrechetProf. M. StachowiakDr. A. OseroffDr. R. PandeyDr. J. MorganDr. P Dandona
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