muhammad ashfaq pakistan barcode project: progress and applications

Muhammad Ashfaq

Pakistan barcode project:progress and applications

2010: Barcoding economically important insect species from Pakistan

Biodiversity Institute of Ontatio National Institute for Biotechnology and Genetic Engineering (NIBGE)

Funded by: HEC

Project execution

Manual catching Pipettes and sieves

Arial nets Light traps Shaking onto sheets

Activities and Progress

Collecting animal parasites

Specimen preparation and databasing

SpecimensAnimals: 34103Plants: 1289

BarcodesAnimals: 28605Plants: 988

IDRC (62%)

18 months

Pakistan-only BINs/ species

II. Plants: 363

Number of BINs/ species

II. Genera: 698

Taxonomic identifications

III. Species: 764

Barcode Applications

Reference libraries

Helicoverpa armigera

Helicoverpa zea (GU087831)

Heliothis virescens (GU336572)

Masalia sp.

Heliothis sp.

Heliocheilus confertissima

Spodoptera littoralis

Spodoptera litura

Spodoptera cilium

Spodoptera exigua

Mamestra sp.

Mythimna sp.

Leucania venalba

Mythimna convecta

Agrotis segetum

Agrotis ipsilon

Chrysodeixis acuta

Thysanoplusia orichalcea

Chrysodeixis eriosoma

Ctenoplusia albostriata

Trichoplusia ni

Hypocala deflorata

Pandesma robusta

Dysgonia torrida

Achaea janata

Earias insulana

Earias vittella

Pectinophora gossypiella

53

99

90

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29

30

89

92

72

41

91

2

21

21

41

18

19

98

33

73

24

23

5

86

49

Heliothinae

Amphipyrinae

Hadeninae

Noctuinae

Plusiinae

Catocalinae

Chloephorinae

Gelechiidae

Noctuid species in/around cotton fields of Punjab

Previously only S. litura was reported from cotton in Punjab

Genetic diversity:

mango mealybug, Drosicha

mangiferae

Bt- 1 (36) [AAM1247] Asia II 1

Bt- 2 (1) [ AAM1246]

Bt- 3 (2) [ACB7718]

Bt- 4 (5) [ AAM1248] Asia II 7

Bt- 5 (2)

Bt- 6 (8) [AAM1243]

Bt- 7 (11) [AAM1245] Asia II 5

Bt- 8 (15) [AAM1244] Asia 1

Bt- 9 (2)

Bt- 10 (2) [AAA4495]

Bt- 11 (13) [AAT8875] MEAM1

Bt- 12 (19)

99

99

99

99

99

97

99

99

59

99

84

63 99

52 58

98

29

40

99

91

0.02

Bemisia tabaci, a complex of cryptic species

E

W

N

S

(A) Asia II 1

(B) MEAM 1

(C) Asia 1

Bemisia tabaci, distribution analysis

Dengue epidemic in Punjab

Culex fuscocephala (5; 0.6) Culex perexiguus (1; -) Culex bitaeniorhynchus (4; 0.15) Culex mimeticus (1; -)

Lutzia fuscana (2; 0.0) Culex tritaeniorhynchus (84; 2.01) Culex quinquefasciatus (1025; 1.05)

Culex theileri (3; 0.0) Armigeres subalbatus (45; 0.31)

Culicidae (5; 0.6) Aedes MA03 (3; 0.9)

Aedes MA02 (9; 1.0) Aedes MA04 (45; 1.4)

Aedes aegypti (48; 0.5) Aedes albopictus (175; 1.3)

Aedes MA01 (6; 0.9) Aedes w-albus (10; 0.9) Ochlerotatus pseudotaeniatus (5; 2.0)

Ochlerotatus sp. (2; 0.0) Anopheles subpictus (39; 1.4)

Anopheles peditaeniatus (8; 0.8) Anopheles pulcherimus (8; 0.3)

Anopheles splendidus (1; -) Anopheles stephensi (29; 0.4)

Anopheles sp. nr. dravidicus (1; -) Anopheles annularis (3; 2.4)

Anopheles culicifacies (5; 0.6) Anopheles culicifacies A (1; -)

Mansonia bonneae (1; -) Chironomus kiiensis

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31

13

10

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96

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Mosquito species in Punjab

Genus: Anopheles Genus: Culex

Genus: Aedes Aedes aegypti Aedes albopictus

Genus-wise distribution of mosquitoes in Punjab

Outreach

Barcoding project at NIBGE

Barcoding team members

Thank you for your attention