nematode extraction methods: foliar extraction techniques

Nematode Extraction Methods:Foliar Extraction Techniques

Kavitha GovindasamyJimmy R.Rich

Maria L .Mendes

A Plant-Parasitic Nematode

Sampling for Nematodes

Sampling and extracting for nematodes serves two purposes

Diagnosis a current problem Predict a future problem

Objectives of sampling and extraction

Identify the nematode Population estimation Diagnose a nematode disease Make management decisions Regulatory purposes Research and surveys

Some Common Nematode Extraction Methods

Foliar samples Punching and incubating Scissors technique Soil samples Baermann funnel Modified Baermann funnel Root Samples Jar incubation and sieving Blending and sieving

Foliar Extraction Techniques:Using a punch or scissors

Principle Nematodes migrate out of the leaves when the lesions are soaked in water

Materials Required

Hole punch or scissorsPetri dish or other container

Cut leaf tissue and place in water

Punch the lesions and place tissue disks in water

Take samples near healthy tissue

Incubate 24 hours and observe under microscope

Acknowledgments Janete Brito, Department of Plant Industry, Gainesville,

Florida Frank Woods, Nematode Assay Lab, University of

Florida, Gainesville, Florida Joey Orajay, Entomology and Nematology Department,

University of Florida, Gainesville, FL

For additional information or comments, please contact:

Kavitha_nem@yahoo.com jimmyr@ufl.edu mlmendes@ufl.edu