ngs needs for s dy cap re · 20100316 pe pe edwmcuppenreseq_mco pe 1 transc1iptomevaroussanñianeke...
Post on 25-Sep-2020
0 Views
Preview:
TRANSCRIPT
NGS needs for study capture
Frans P Ruzius
Hubrecht Institute
NGS
• Next Gen(eragion) Sequencing
• Deep Sequencing
– Small reads
• 50-500 bp
– Lots of reads
• 0.5 – 800 M
– Big reference
• Genome
– 3 Gb
Sequencing run
Experiment webpage
Sequencing run
Experiment webpage
Sequencing run
Mapping pipeline
Sequencing run – future planns
Submission website
Sequencing run
Need for sample tracking
Most heard questions in the lab
• Can this be solved by study capture?
– Is my data already analyzed?• What is already mapped and what not?
– Where can I find my data?• Where is the data stored?
– From which run was my library?
– What was the mappability?
top related