paul klapper clinical virology manchester royal infirmary hepatitis c dried blood spot testing

Paul Klapper

Clinical Virology

Manchester Royal Infirmary

hepatitis C dried blood spot testing

- eluate – automated test system

HCV antibody detection

HCV antibody confirmatory testing

- HCV RNA

- HCV genotype

dried blood spot

HCV antibody detection - sensitivity - 100% - specificity - 100%

HCV antibody confirmatory testing - sensitivity 100% - specificity 100%

how good is it?

HCV RNA - sensitivity 96% - specificity 100%

HCV genotype - sensitivity 96% - specificity 100%

dried blood spot

HCV antibody detection - sensitivity - 100% - specificity - 100%

HCV antibody confirmatory testing - sensitivity 100% - specificity 100%

how good is it?

HCV RNA - sensitivity 96% - specificity 100%

HCV genotype - sensitivity 96% - specificity 100%

[orasure 91.7%; 99.2%]

[orasure - not available – 33-88%]

dried blood spot

but that’s’ not all….

using same sample

HBsAg – confirmation and full markers(sensitivity 96%)

HBc Ab – (sensitivity 100%)HIV testing – HIV antibody/antigen and confirmation (2 additional assays)

(sensitivity 98%)syphilis – good for infectious syphilis (sensitivity 100%)

not so good (sensitivity 50%)for detection of syphilis at any time

turnaround

working toward 24 hr turnaround time – these samples a little longer (overnight elution)

Web – secure server for results access

dried blood spot