area for rbc counting - judoctors « medicine lectures ... · physiology lab #1 rbc...
Post on 08-Mar-2018
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Physiology lab #1… RBC counting
Hemocytometer:
It’s a kind of glass slide, we’ll use it in this lab as well as the coming one.
the area of RBC counting in which we’re concerned has the following
dimensions and divisions :
You counted the RBC’s in a volume of 0.004 mm^3, remember that you
diluted the sample now for the calculations ( assuming that you counted
120 RBC\0.004 mm^3 ):
o 120 * 25 = 3000/0.1mm^3 … (i.e. in the whole RBC counting area)
o 3000 * 10 = 30000/mm^3 … diluted
o 30000*200(the dilution factor)= 6,000,000/mm^3 … not diluted
Those are notes I’ve written in the lab
..they’re particularly useful for those
who did not attend it, they include
extra things about the methods only
Area for RBC counting
Physiology lab #2… WBC counting
Each of the 4 squares used in WBC counting has a volume of:
1 mm * 1mm * 0.1 mm= 0.1 mm^3
Count WBC’s in each then take the avg of them
For dilution : 1ml blood in 19 ml saline dilution factor = 20
You counted the WBC’s in a volume of 0.1 mm^3 , remember that you diluted
the sample now for the calculations ( assuming that you counted
40WBC\0.1 mm^3 ):
o 40 * 10 = 400/mm^3 … diluted
o 400*20(the dilution factor)= 8000/mm^3 … not diluted
Areas forWBC counting(bold borders)
Depth = 0.1 mm
1 mm
1 mm
Physiology lab #3
I. Hb concentration
o Take 20 microliter of blood .
o Add 2 -3 drops of HCL
o You’ll get “ferrohematinic acid” , dark brown color
o Dilute it with distilled water till you get the same color as the standard
solution
o Then check the grade on the tube
II. HcT…. H1/H2 * 100%
Centrifuged blood sample
Heig
ht 1
Heig
ht 2
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