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Plants Interacting
with Pathogens
Walter Gassmann
The cellular basis of plant immunity
J L Dangl et al. Science 2013;341:746-751Published by AAAS
PAMP-triggered
immunity
Effector-
triggered
immunity
The IPG plant-microbe interactome (simplified)
Brown
English
Gassmann
Heese
Koo
Korkin
Mihail
Mitchum
Peck
Schoelz
Stacey
Zhang
Tools
genetics/genomics
molecular biology
biochemistry
cell biology
spectrometry
computational biology
Process
PTI
ETI
pathogenicity
hormones
regulation
Plant
Arabidopsis
soybean
Nicotiana
other
Microbe
bacteria
fungi
viruses
nematodes
other
The IPG plant-microbe interactome (simplified)
Brown
English
Gassmann
Heese
Koo
Korkin
Mihail
Mitchum
Peck
Schoelz
Stacey
Zhang
Tools
genetics/genomics
molecular biology
biochemistry
cell biology
spectrometry
computational biology
Process
PTI
ETI
pathogenicity
hormones
regulation
Plant
Arabidopsis
soybean
Nicotiana
other
Microbe
bacteria
fungi
viruses
nematodes
other
What is the mechanism underlying polar growth of Agrobacterium tumefaciens?
Polar attachment of Agrobacterium tumefaciens cells to a root hair
Crown gall tumor caused by Agrobacterium tumefaciens
The Brown lab (brown.biology.missouri.edu) is interested in understanding the mechanism of polar elongation in Agrobacterium tumefaciens
New growth (unlabeled) of the Agrobacterium tumefaciens cell occurs at the cell pole
We expect to apply insights gained from this basic research to address:
- the need for biocontrol of this plant pathogen - for improved genetic engineering of plants
Pam Brown
1. P6 forms inclusion bodies that are considered “virion factories”. Almost all CaMV virions are retained with the P6 inclusion bodies.
EM of P6 inclusion body adjacent to chloroplast
2. P6 inclusion bodies interact with and move on actin
microfilaments. Harries et al. 2009, Plant Phys.
P6 protein tagged with GFP.Actin tagged with dsRed
3. P6 protein physically interacts with CHUP1, a protein that moves
chloroplasts on microfilaments. Angel et al. 2013, Virology.
CHUP1, tagged with CFP, is localized to the outer membrane of chloroplasts, which autofluoresce red
CaMV P6 is a multifunctional protein, with distinct roles in translation of viral proteins, silencing suppression, defense gene suppression, and trafficking of CaMV virions to plasmodesmata.
Jim Schoelz
FLS2
?
How Does PAMP RecognitionLead to Host Resitance?
??
0 min 8 min2 min
388.1
535.1
633.2
672.2
770.3
100 200 300 400 500 600 700 800
m/z
yΔ6438.2
272.0
y1
175.4
y6
y5
y2
510.5461.9
b3b2251.0
I H H P P pS P R
y5 y4 y3 y2 y1y6
b2 b3
yΔ5
yΔ4
yΔ3
yΔn= yn – H3PO4
y3
Scott Peck
High Affinity (~1 nM) Chitin and Lipo-Chitin Signaling Pathways in Plants
X
Symbiotic NF receptor
Chitin
Chitooctaose receptor
X
Non-symbiotic NF receptor
ALL PLANTS SYMBIOTIC PLANTS
Induction of plant
defense pathways
Suppression of
plant innate
immunity
Induction of
symbiotic
development
Gary
Stacey
How Do Cyst Nematodes Reprogram Host Cells?
Mitchum et al (2012) Annu Rev Phyto Melissa Mitchum
DGA
CytoplasmicEffectors
RCR3ApoplasticEffectors
β-1,3-G
SPS Hs10A06 Hg/Hs30C02
GrVAP
Ma Gro1-4 Hero A Gpa2 Cf-2Mi9Mi-1.2
GpRBP1
GrSPRYSEC19
MiCRT
Rhg4SHMT
Hs1Pro-1
Rhg1
α-SNAP WI12AAT
WRKY72
WRKY70
Basal Defense
NB
TIR
TM
CC
LRR
GrCEP12
Nucleus
HSP90
Mechanisms of Plant Resistance to Nematodes
Liu, Kandoth et al., Nature (2012) 492: 256-260
Kandoth & Mitchum, COPB (2013) 16: 457-463
Jasmonate Metabolism and Signaling
Plant resistance to various biotic stresses are controlled by lipid-derived hormone jasmonate. Metabolic processes involved in maintenance of optimal hormone level as well as its control over global gene expression are studied using multidisciplinary approaches including mass spectrometry.
UPLC-MS/MS
Abraham Koo
Constitutively activated immunity is deleterious to
the plant
Columbia-0 srfr1-4
SRFR1 encodes a novel TPR protein
that may function as an adaptor protein
TPR
srfr1-1 srfr1-2
"conserved domain of
unknown function"
1052 aa
AtSRFR1MtOsDdEhHsTTC13MmTnSpCeOGT1DmOGTAtSECAtSPYScSSN6AtSGT1b
SRFR1-
like
TPR
TGAATG7400*
srfr1-1
*srfr1-2
GFP-SRFR1 + RFP
TCP
TF
promoterdefense gene
Kim et al., unpublished
SRFR1 is a nucleocytoplasmic adaptor protein that
negatively regulates the plant immune system
TNL
TNLRPS6
AvrRps4
immunity
HopA1
EDS1RPS4SNC1
SRFR1
Bhattacharjee et al. (2011) Science
Nimchuck et al. (2003) Annu. Rev. Gen. 37: 579
adapted from Tao et al. (2003) Plant Cell 15: 317
SRFR1 may determine the sensitivity of the plant
effector-triggered immune response
srfr1
The cellular basis of plant immunity
J L Dangl et al. Science 2013;341:746-751Published by AAAS
PAMP-triggered
immunity
Effector-
triggered
immunity
AcknowledgmentsGassmann lab:
Sang Hee Kim
Fei Gao
Sharon Pike
Morgan Halane
Chris Garner
Ben Spears
Ellie Nguyen
Courtney Coleman
Ru Dai
Yang Liu
Saikat Bhattacharjee, Regional
Centre for Biotech, India
Daniel Nam, Texas State
Xuecheng Zhang, MU
Soon Il Kwon, Korea Univ.
Jessica Koczan, MSU
Hiroki Osawa, Tokyo Univ.
Jae-Jong Noh
Dipanwita Saha, MU
Nkemdi Anyanwu, MU
Amber Moore, Tuskegee
Joe Adiasor, Mo S&T
Collaborators:
MU
Michael Garcia, Stephen Shannon
Gyeongsang Natl. University/MU
JC Hong, Geon Hui Son
Cornell
Jian Hua
MPIZ Köln
Jane Parker
Katharina Heidrich
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