preparing client

Preparing client to microbe inspection

Oleh :

Dr. Jacky Junaedi

Introductiom

Kind of specimen sputum, blood, feces, and urin

At least one time did

What for?- diagnosis- bacteria/virus/parasite, etc- terapeutic responses

SPUTUM

SPECIMEN REMOVAL SPUTUM

The best in the morning Brushed teeth first Rinse the mouth Cough to get the sputum 15-30cc, First cough sputum

PlacedSaving less than 24 hour in room

temperatureSterrile pot placed

ShippingShipping < 2 hour in room

temperatureTransport medium (Amies

medium, Stuart’s medium)

STUART’S MEDIUM AMIES MEDIUM

Blood specimen inspection

Blood specimen:

Hematology inspection Chemical inspection

Serologin and immunologic inspection

Parasitologic and blood cultureWIDALTEST

Swab the vein superficial place with 70% alcohol

apply the torniquet 45 degree manuver of spuit, pull the

blood Put the blood to the liquid media TSB

(Trypticase Soy Broth)

SPUIT

TSB

Specimen removal

Vein blood

10-20cc

The best at febris

PlacedSaving less than 24 hour in room

temperatureSterrile placed

ShippingShipping < 2 hour in room

temperature

feaces specimen inspection

Kind of inspection Bloody feaces????? Parasite egg Bacterial andn virus

Specimen removal

handscoon Amount of feaces

2,5 cm solid feaces 15-30 mL liquid feaces

For feaces culture use a sterrile swab

SWAB

Placed Less then 1 hour in room temperature

Stuart’s medium, ataupun Pepton water Saving < 24 jam room temperature, > 24 jam at

4°C

Shipping Less then 1 hour in room temperature

STUART’S MEDIUMPEPTON WATER

TETRA THIONATE BROTH

Urine specimen inspection

(clean-catch or midstream urin specimen)

Urinalisis and microbe inspection

KATETER

Specimen removal:

Clean up the oue (penis or vagina)

Collect the mid stream urine

30-60mL

Place and shipping

Less then 1 hour at room temperature

Refrigerator store less then 24 hours

Use urine transport tube (sterrile)

STERIL CONTAINER

URINE TRANSPORT TUBE