quick test xm - abrint.net · 1 xm quick test 1,2 ml 0,5 2 1,2 ml with blood collector strip 0,5...

XM Canine PROCEDURETEST

N°1: PREPARATION OF BLOOD SAMPLES (MAJOR = Donor RBC + Recipient serum)

Recipient Donor

Serum or plasma pRBCs

(packed Red Blood Cells)

Whole blood• EDTA• ACD

Do not use Heparin

• CPD• Plain tube

Centrifuge blood tube in order to collect serum or plasma.

Centrifuge blood tube.Discard the serum or plasma to collect pRBCs.

OR

Collect blood from blood bag segment.

4

pRBCs

Mix gently & incubate 10’ at room temperature

N°2: INCUBATION AND WASHING PROCEDURE

1

4

In the clean test tube

10 min

1 Wash Buffer

1 white top buffer 1

1 Blood collector strip

2 large pipettes

1 small pipette

Material provided:

1 green top buffer 2

1 Clean test tube (1,5ml)

2Bu�er

1Bu�er

R D

XM

1

lot: 154684769.25.63Exp: 2009.06 Pat :

Date :

1 XM Quick Test

1,2

ML

0,5

1,2

ML

0,5

1,2

ML

0,5

2

1,2

ML

0,5

1,2

ML

0,5

1,2

ML

0,5With blood collector strip

Donor’s tubeOR

Donor’s blood bag segment

EDTA

D

3

Recipient’s tube

With the small pipette

3Serum or plasma

R

in the test tube

1,2

ML

0,5

1,2

ML

0,5

1,2

ML

0,5

1 Empty well

1,2

ML

0,5

1,2

ML

0,5

1,2

ML

0,5

7s

1,2

ML

0,5

1,2

ML

0,5

1,2

ML

0,5

MAJOR

lot: 154684769.25.63Exp: 2009.06 Pat :

Date :

XM

5

A

Suspension washing procedure

XM

CB

1,2

ML

0,5

1,2

ML

0,5

1,2

ML

0,5

1,5ML

With a large pipette

2min

1,2

ML

0,5

1,2

ML

0,5

1,2

ML

0,5

Don’t forget the balance tube(not provided)

1,2

ML

0,5

1,2

ML

0,5

1,2

ML

0,5

Discard the supernatant

Add

Centrifuge

1000 g or2300 rpm

STEP 5 MUST BE DONE 3 TIMES

N°3: XM TEST PROCEDURE

1 2

3

3

1,2

ML

0,5

1,2

ML

0,5

1,2

ML

0,5

4 5

lot: 154684769.25.63

Pa

t :

Da

te :

6

lot: 154684769.25.63

Pa

t :

Da

te :

2 to 5min

WAIT UNTIL COMPLETE MIGRATION

7

lot: 154684769.25.63Exp: 2009.06

Pa

t :

Da

te :

!

+

lot: 154684769.25.63Exp: 2009.06 Pat :

Date :

lot: 154684769.25.63Exp: 2009.06 Pat :

Date :

lot: 154684769.25.63Exp: 2009.06 Pat :

Date :

lot: 154684769.25.63Exp: 2009.06 Pat :

Date :-

lot: 154684769.25.63Exp: 2009.06 Pat :

Date :

C = control

: www.alvedia.com - contact@alvedia.com

Contact us to ask for the

lot: 154684769.25.63Exp: 2009.06 Pat :

Date :

Result Interpretation

The XM test line will often be weaker than the control line

Collect entire suspensionwith a large pipette.

Fill the well.

Read immediately after testing.

1,2

ML

0,5

1,2

ML

0,5

1,2

ML

0,5

lot: 154684769.25.63Exp: 2009.06 Pat :

Date :

And mix gently

2

1,2

ML

0,5

1,2

ML

0,5

1,2

ML

0,5

1,2

ML

0,5

1,2

ML

0,5

1,2

ML

0,5

Mix gently

Incompatibility Compatibility