recipe meeting, charquemont october 23, 2003

RECIPE meeting, CharquemontRECIPE meeting, CharquemontOctober 23, 2003October 23, 2003

Andy Siegenthaler

Swiss Federal Research Institute WSL

Antenne romande

CH-1015 Lausanne / Switzerland

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Clo

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La Chaux d’Abel / Le RusseyLa Chaux d’Abel / Le Russey

Le Russey

La Chaux d’Abel

Bog regeneration typology of the Jura - Bog regeneration typology of the Jura - Thesis Thesis from Yvan Matthey, 1993from Yvan Matthey, 1993

• Yvan Matthey’s work concerned the description of 369 situations along the Jura chain. He used factorial analyses using 6 common environmental descriptors.

• He describes three progressive series which differentiate into two independent (aquatic and terrestrial), and two parallel terrestrial progressive (changing) series. The ultimate stages of the 3 series’ evolutions show an obvious physiognomic “uniformisation” of the environments.

Terrestrial:

1) Hummocks’ serie (Eriophorum vagintum)2) Moss-lawns’ serie (Polytrichum strictum and Polytrichum commune)

Aquatic:

1) Trembling marsh (Potentilla palustris …Phagnum magellanicum)

• He also points out the importance of the mosaïc aspect in the early stages.

At la Chaux d’Abel, we have a mixture of the two terrestrial situations that appear in mosaïc plus a gradient in the progressive stages.

Choice of keystone species (WPI & WPII)Choice of keystone species (WPI & WPII)

Question: common plant species in order to compare climatic gradient (at

least Sphagnum sp + 1 specie)?

Baubt: Eriophorum angustifolium, Eriophorum vaginatum ? Triglochin sp?, Molinia caerulea?

Finland: Sphagnum sp, Eriophorum vaginatum, …?

Chaux d’Abel / Russey: Sphagnum sp, Eriophorum vaginatum, E. angustifolium and Polytrichum strictum

Scottland: Sphagnum sp, Eriophorum vaginatum, …?

0

10

20

30

40

50

60

34

5

7

2

134

34

34

7 7 7 7

=

6

6

34

6

6=

Old peat

1

2

3

4

5

6

7

Vascular plants’ litter

Vascular plants’ stems and leaves

Mosses or peat: 0<h≤5 cm below surface

Mosses or peat: 5-10 cm below surface

Peat: 12.5-17.5 cm below the surface

Peat: 22.5-27.5 cm below the surface

Peat: 32.5-37.5 cm below the surface

5

5

5

New peat

Nb of samples:

Sub-sampling strategy I and II in various situations

4(6) 5(7) 5(7) 4(6) 3(5)

2

1

6 Median water table

=

34

7

5

6

8

Peat: 42.5-47.5 cm below the surface8

34

7

5

6

8

34

7

5

6

8

34

7

5

6

8

34

7

5

6

8

4(6) 5(7) 4(6) 4(6) 4(6)

I II

c

I. Semi-systematic II. Systematic Taken for the analyses (i.g.)

c

some analyses

some analyses

The trenches at le RusseyThe trenches at le Russey

H = max. = old surface peat

H = mediane

H(water level) = min. = -33 cm

Filing up 50 cm

Buffer zone of 3.50 cm between trenches

Low water level: upper part of the pot 30 cm above the highest H

Intermediate water level

High water level: upper part of the pot 10 cm above the median H

Scale 100%

Sca

le 2

00%

11 m

Peat in place

Free water

If necessary, put a lateral drain to evacuate the water if the trenches fills up

50 cm85cm cm

Level zero

This part has to be filled up with porous peat This part has to be filled up with porous peat in order to allow for a horizontally mowing in order to allow for a horizontally mowing ground water levelground water level

Assignment of tubesAssignment of tubes

10 cm 10 cm 10 cm

30 cm 30 cm 30 cm 30 cm

5 cm 5 cm

15 cm 15 cm 15 cm 15 cm

L 1 L 2 L 3

Sp 1 Sp 2 Sp 3Sp 0

L 0

Year 1 Year 2

Sp 1 Sp 2 Sp 3

Sp 1 Sp 2 Sp 3Sp 0

Spare species; litter should not be a problem over two yearschamber

tube

Enough space for handling the corer by two persons1.2 m

Slope

Water level I

Water level II

Water level III

Total = 15 pots x 3 water levels x 3 replicates = 135 pots, from which only 72 will be analysed!

We would decide to continue for another year looking at some plant-pots. 3 m

1 out of 3 trenches (= replicates) WP 2: Species

WP3: Litter

The water levels in the trenchesThe water levels in the trenchesFosse A

-100

-80

-60

-40

-20

0

0.00 200.00 400.00 600.00 800.00 1000.00 1200.00niveaux zˇro en cm (sommet des piquets-sol)

MIN 2

MAX 2

MEDIANE 21

fosse B

-140-120-100

-80-60-40-20

0

0.00 200.00 400.00 600.00 800.00 1000.00 1200.00 niveaux zˇro en cm (sommetdes piquets-sol)

MIN 2

MAX 2

MEDIANE 2

fosse C

-200

-150

-100

-50

0

0.00 200.00 400.00 600.00 800.00 1000.00 1200.00 niveaux zˇro en cm (sommet des piquets-sol)

MIN 2

MAX 2

MEDIANE 2

Temperature profiles - situation A Temperature profiles - situation A October ‘03 October ‘03

-120

-100

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-20

0

20

Dep

th /

cm

5.5 6 6.5 7 7.5 8 8.5 9 9.5 10

Temp. /°C

CA-A6

CA-A5

CA-A4

CA-A3

CA-A2

CA-A1

Temperature profiles - situation BTemperature profiles - situation BOctober ‘03October ‘03

-120

-100

-80

-60

-40

-20

0

20D

epth

/cm

5.5 6 6.5 7 7.5 8 8.5 9 9.5

Temp. /°C

CA-B6

CA-B5

CA-B4

CA-B3

CA-B2

CA-B1

Temperature profiles - situation CTemperature profiles - situation COctober ‘03October ‘03

-120

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0

20D

epth

/cm

6 6.5 7 7.5 8 8.5 9 9.5 10

Temp. /°C

CA-C6

CA-C5

CA-C4

CA-C3

CA-C2

CA-C1

Mean temperature profiles - October ‘03Mean temperature profiles - October ‘03

6

6.5

7

7.5

8

8.5

9

9.5

10C

ell

Mea

n f

or T

emp

. /°

C

a b c d e f g h i j k l m

CBA

Depth /cm

Temperature profiles between Mai and Temperature profiles between Mai and June ‘03 at CA-2June ‘03 at CA-2

-160

-140

-120

-100

-80

-60

-40

-20

0

20

Dep

th /

cm

4 6 8 10 12 14 16 18 20 22 24Temp /°C

Group for 5. 6.2003

Group for 16. 5.2003

Temperature profiles between Mai and Temperature profiles between Mai and June ‘03 at CA-6June ‘03 at CA-6

-300

-250

-200

-150

-100

-50

0

50

Dep

th /

cm

2.5 5 7.5 10 12.5 15 17.5 20 22.5 25 27.5Temp /°C

Group for 5. 6.2003

Group for 16. 5.2003

Mean temperatures (ANOVA and Fisher’s Mean temperatures (ANOVA and Fisher’s PLSD) - October ‘03PLSD) - October ‘03

5.5

6

6.5

7

7.5

8

8.5

9

9.5

10

Tem

p.

/°C

A B C

a b a/c

Comparisons of the 3 situations in Comparisons of the 3 situations in October ‘03 (ANOVA and Fisher’s PLSD)October ‘03 (ANOVA and Fisher’s PLSD)

a b a/ca ca/b

-22.5

-20

-17.5

-15

-12.5

-10

-7.5

-5

-2.5

0

2.5

WT

dep

th /

cmA B C

3.5

3.6

3.7

3.8

3.9

4

4.1

4.2

4.3

4.4

pH

A B C

Evolution of the pH with depth and sites’ Evolution of the pH with depth and sites’ comparison - Sept.’03comparison - Sept.’03

-50

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-25

-20

-15

-10

Dep

th/c

m

4.4 4.5 4.6 4.7 4.8 4.9 5 5.1 5.2pH (sept)

CA5

CA2

Measures of CO2 with depth using Measures of CO2 with depth using peepers - Sept ‘03peepers - Sept ‘03

-60

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-25

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-10

Dep

th/c

m

0 5000 10000 15000 20000 25000 30000 35000ppmv CO2

CA5

CA2

Measures of CH4 with depth using Measures of CH4 with depth using peepers - Sept ‘03peepers - Sept ‘03

-60

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-45

-40

-35

-30

-25

-20

-15

-10

Dep

th/c

m

-1000 1000 3000 5000 7000 9000ppmv CH4

CA5

CA2

Parallel evolution of CO2 and CH4Parallel evolution of CO2 and CH4

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-60

-55

-50

-45

-40

-35

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-25

-20

-15

-10

Dep

th/c

m

-5000 5000 15000 25000 35000X Variables

ppmv CH4: CA5

ppmv CH4: CA2

ppmv CO2: CA5

ppmv CO2: CA2QuickTime™ et un décompresseur TIFF (LZW) sont requis pour visionner cette image.

CA-5(zone C) CA-2(zone A)

Legend to Amélie’s figuresLegend to Amélie’s figures

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Total « Respiration » - (CHTotal « Respiration » - (CH44 + CO + CO22) /ppmv

-60

-55

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-10D

epth

/cm

0 10000 20000 30000 40000Resp. totale

CA5

CA2

Peat sampling - use of a cartridgePeat sampling - use of a cartridge

•PET sheet of 50 cm long, hold in place with a stop-piston, capped at the bottom

•minimise the duration of time at which the anoxic peat can be oxidised

•less manipulations on the field

•makes the transportation easier

•enables to store the samples for some time until they can be processed

•Preparing the cartridges doesn’t take long

Anoxic slicing of coresAnoxic slicing of cores

•Soft gas-chamber that allows to sample in a

O2-free environment

•The advantage is that the bag’s air can be

emptied before filling with N2-gas - it takes

less gas to purge•Easy to make, can be done by all the

dispatchers and could even be used in the

field

•There should be a small N2-flux and we

could use PEWA strips to control if there is

no oxygen left.•Question: 1) Where do we cut the corer? 2)

Do we need to separate the oxic part before

introducing the core into the chamber? Who

needs preserved samples?

Wooden-peepers for gas, cations and Wooden-peepers for gas, cations and anions measurementsanions measurements

•These wooden logs would replace Philipp Steinman’s Plexiglas model

•The wooden-peepers would be inserted where we extract the cores - this would ensure a 1 to 1 wedging

•There would be plastic pots (diam. 50 mm) with a polysulfon membrane (0.2 µm) on two or four sides (depending on the needs)

•The pots can be placed at different depths (0-5, 5-10,…cm), at convenience

•More solution can be retrieved (about 100 ml for one pot) than in the Plexiglas model

•Should be easier to handle

•Question: who does the analyses (PhS, AJF, AS)? (see Philipp’s table)

Fast fluorescence measurements - EC-Fast fluorescence measurements - EC-BERI projectBERI project

The fluorescence transientThe fluorescence transient

from Strasser et al. 2000

from Strasser et al. 2000

Example Eriophorum - BERIExample Eriophorum - BERI

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