reference standards dna & ffpe...

Reference Standards

DNA & FFPE Samples

Mutation Cell Lines

CRIPSR-Cas9 for Footprint-Free Genome Editing

Homologous

Directed Repair

Custom Cell Line Engineering

Cell Type Modification Type

Cancel Cell Lines

KO

Point Mutation

Insertion

Human iPSCs

KO

Point Mutation

Insertion

Human ES Insertion

Primate iPSCs Insertion

Human Primary CellsKO

Insertion

Human Fibroblasts Point Mutation

Mouse Fibroblasts Point Mutation

Rat Thycrocytes KO

Selected lines from >100 distinct parental cell lines engineered:

A-549 BEAS-2B BT-474 HaCaT HBE Huh7 MCF-10A OCCM-30 RPE-1

SK-MEL-31 Tert-RPE U-2 OS 786-O CHLA-10 A-375 Gist-T1 DLD-1

HCT-116 HEK293 HEK293T HeLa HepG2 4T1 C2C12 cTEC ES Cells

iPSCs MWCL-1 BCWM-1 H929 Jurkat K562 KHYG-1 LAD2 MM.1s MWCL-1 NCI-H929 T2 cells TF-1 HT1080 HT29 KBM-7 KM12-Luc LnCaP MDA-MB231 NCI-H2228

RKO TC32 SCC35 SH-SY5Y

>400 Gene Edited Cell Lines Generated

Isogenic Cell Line – Engineering Workflow

• Clonally Homogeneous

Isogen

ic Pair

• Genetically Validated

• Homozygous Modification

Bio-Relevant Specimens

FFPE Cell Blocks

Tumor Xenografts

Amplicons

Plasmids

Genomic DNA

Engineering Clinically Relevant Mutations

Example 1: Point Mutation Example 2: Small Insertion Example 3: Deletion

150+ point mutations engineered across 15+ genes

KRAS EGFR BRAF NRAS HRAS PIK3CA IDH2 FGFR3 TP53 ESR1 FLT3 APC CTNNB1 PTEN BRCA1

Focused on MAPK Pathway

Exon 19 Mutation Panel

• 22 Deletion Mutations• In-frame mutations that occur within kinase domain• Target TKI’s therapeutics• 21 of 22 mutations are unique to ASC

114 Off-the-Shelf Mutations

Molecular Reference Standards

Assay Development • Optimize sample processing protocols• Establish limit of detection (LOD)• Identify and validate optimal reagents• Monitor the impact of workflow changes on results

Quality Control and Assay Reproducibility • Accurately evaluate batch-to-batch variability • Easily transfer workflows across labs or institutions

CE-Seq

NGS PCR

FISH

IHC

Reference Standards

Largest Cell Line Panel of EGFR Mutations

Molecular Reference Standards – FFPE Blocks and Slides

Intra-Block Inter-Block

Hematoxylin & Eosin Stained

Hematoxylin Stained

Apps

Molecular Reference Standards – FFPE Scrolls

Apps

• Simulate processing of biological specimens • Perfect for optimizing DNA extraction or

assay development

BRAF V600E TaqMan Assay

FFPE Scrolls

Tailored to your assay development needs

EGFR T790M mutant cell lines fixed and diluted into WT parental cells at targeted allelic frequencies of 50%, 10%, and 1% T790M

Accelerate Discovery Through Innovative Life Science