review 3 c483 spring 2013. net outcomes of oxidative phosphorylation (shuttles) fatty acid...

Review 3

C483 Spring 2013

Net outcomes of

• Oxidative phosphorylation (shuttles)• Fatty acid catabolism• Nitrogen catabolism• Light reactions of photosynthesis• Calvin cycle

Structures to know

• All amino acids• (deoxy)ribonucleosides and

(deoxy)ribonucleotides• Carbamoyl phosphate and urea• Pyruvate, oxaloacetate, a-ketoglutarate• PRPP

Metabolic Pathways/Reactions

• Know these reactions/pathways (not arrow mechanisms)– Beta oxidation– PLP-mediated transamination– Formation of carbamoyl phosphate– Catabolism of amino acids covered in lecture– Basic hydrolysis and RNase-catalyzed hydrolysis of

RNA

Protonmotive Force

• NADH + H+ + ½ O2 NAD+ + H2O + 10 H+ pumped

• succinate + ½ O2 fumarate + H2O + 6 H+ pumped

-O

NH2

O

-O

O

O

-O

O

O

COO-

-O

NH2

O

COO-

NH

O

R O-

NH

H2N

R O-

+

NH

O

R O-

+

-O

O

O

COO-

-O

NH2

O

COO-

-O

NH

O

COO-

NAD(P)+ NAD(P)H

H2O

+ NH3

O

NH2H2N

bicarbonate

ammonia(from glutamate)

aspartate(can be derived from glutamate via transmination)

O

O

O

O

O

O

O

O

O

O

O

O

O

OO

O

OH

P

O

O

O

NH

O

ONO

OHOH

HH

HH

OP-O

O-

O

NH

O

ONO

OHOH

HH

HH

OP-O

O-

O

F

Photosynthesis: Net Reaction

2 H2O + 4 NADP+ + 8 photons O2 + 4 NADPH

Proton Gradient

• For every ONE O2

– 4 e- passed– 8 photons absorb– 12 H+ pumped

• In plants, 12 H+ required to turn ATP synthase– 3 ATP made

• Quantum yield is 3 ATP/8 photons or ATP/2.7 photon