rna$extrac+on:$trizol -...

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RNA  Extrac+on:  Trizol

RNA  Extrac+on:  Trizol

RNA  extrac+on  from  cells,  +ssues,  organs

Mammalian  +ssues  (DEPC  treated)

RNA  Extrac+on

RNA  Analysis

OD260/OD280:  1.9-­‐2.1                  GOOD  <1.8                            Protein  contamina+on  >2.2                            RNA  degreda+on    OD260/OD230:  <2.0                            Trizol  contamina+on

RNA  Electrophoresis

mRNA  prepara+on

Reverse  Transcrip+on  PCR

Hybridiza+on

Northern  Blot

Northern  Blot

RNA  in  situ  hybridiza+on

RNA  in  situ  hybridiza+on

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