sampling during prosope for studying picophytoplankton diversity frédéric partensky, dominique...
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Sampling during PROSOPE for studying
Picophytoplankton Diversity
Frédéric PARTENSKY, Dominique MARIE and Laurence GARCZAREK
“Océanic Phytoplankton” Research TeamStation Biologique de Roscoff
CNRS et Université Paris 6
La Thalassa
Position of Stations during PROSOPE
Long term stations
Connection by Netscape (not Explorer)
http://www.obs-vlfr.fr/jgofs/html/ prosope/home.htm
Ask Hervé Claustre for password
PROSOPE web site
Measurement Directory name
profiles number
sample number
Submitted Validated Data avail. Responsible
Nitrates nut 27 (12/05/2000)
Raimbault
Nitrites nut 27 (12/05/2000)
Raimbault
Phosphates nut 17 (12/05/2000)
Moutin
Silicates nut 24 Quéguiner
Biogenic silica biosi 19 Quéguiner
Iron iron 1 8 Ridame, Guieu
Alkalinity ak 20 Begovic, Copin
Salinity sal 72 Tailliez
O2 oxy 60 Begovic, Copin
DMS dms 7 70 Belviso
DMSP dms 7 138 Belviso
HIAC hiac 108 Sciandra
Pigments pig 70 18 Ras, Claustre, Marty
Flow Cytometry cyto 100 Marie
Phytoplankton phyto 22 Quéguiner
Ciliates cilates 12 5 Dolan
COP, NOP, POP pom 21 Raimbault
TOC toc 35 337 Sempéré
NOD, POD mod 15 337 Raimbault
Particulate matter Absorption
apm 70 Oubelkheir, Claustre
Sampling during PROSOPE
Flow cytometry
Short term stations : 1 profile 0-200 m & 1 deep profile (virus/bact.)
Long term stations (MIO, DYF) : 1 profile 0-200 m every 3h for 4 days
Sampling for Diversity DNA : pre-filtration on 3µm, filtration 2-5L on Supor 0.45 µm, freezing in DNA/RNA bufferDiversity Prochlorococcus : Filtration 2 L, filter cut in 2 and frozenHPLC : filter 3 µm (Stations 1, 3, 5, 9, MIO, DYF)TEM : 5-6 grids/depth (Stations 1, 3, 5, 9, MIO, DYF)Cultures : 4/depth (2 < 0,6 µm in P2+/-NO3; 2 < 3 µm in K/10 or K/100)
Station UPW : 2 depthsStation 1 : 2 depths (3 & 60 m)Station 3 : 6 depths (5, 25, 55, 80, 95, 110 m)Station 5, MIO, 7, 8, 9, DYF : 5 depthsStation DYF : 5 depths + détail Chl maximum (45, 50, 55, 60 et 65 m)
Sampling for nutrient status (D. Scanlan, A. Post)
UPW, 2, 4, 6, MIO, +/- DYF : 3 depths
Automatic sampler for collecting
samples during transit
Sampler
Electronic control
After Jacquet et al., 1998
Concentration of Picophytoplankton along the nutrient gradient from Station 1 to MIO
Variation of integrated concentrations of picoplankton over the nutrient gradient
Station
1 2 3 4 5 6 MIOCon
centr
ati
on c
ellu
lair
e in
tegré
e (C
ell.cm
-2)
107
108
109
1010
1011
ProcSynEucBact
A Preliminary Study of the Diversity of Natural Populations of Prochlorococcus
Vertical profiles
Unpublished data D. Marie, D. Tailliez, P. Raimbault
Genetic Characterization of Prochlorococcus Strains using
PCR-RFLP with pcb as a Marker GeneF
luor
esce
nce
(R
elat
ive
Un
its)
0
1
400 450 500 550
Wavelength (nm)
Ecotypes LL
SS120
NATL2
Ecotypes HL
TAK9803-2 (HLII)
MED4 (HLI)
DV-Chl a DV-Chl bMED4
SS120TAK9803-2
NATL2
after Garczarek et al. (2000) Proceed. Natl. Acad. Sci. USA
M
A Preliminary Study of the Diversity of Natural Populations of Prochlorococcus
PCR-RFLP
M1 5 25 55 80 95 110 M2 5 25 55 65 95 M1 5 30 50 90 150 M2
St. 3 St. 5 St. 6
?
Unpublished dataL. Garczarek, A. Dufresne and F. Partensky 0,1
0,2
0,3
0,4
0,50,60,7
?
5 25 55 80 95 110 5 25 55 65 95 5 30 50 90 150
0,2
0,4
0,6
0,8G3 = HL
Génotypes
G4 = LL (deep)
Kb
St. 3 St. 5 St. 6
G5 = LL ? (mid depth)
G2 = HL
A Preliminary Study of the Diversity of Prochlorococcus Interpretation of PCR-RFLP
G1 = HL
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