searching the ncbi databases
Post on 04-Jan-2016
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Searching the NCBI Databases
Search for CFTR[gene] in the Protein database
What is it?
Limit to genes in human onlyCFTR[gene] AND human[orgn]
How many?
Restrict to RefSeq
How many?
RefSeq records can also be filtered using the Limits tab
Examine the NP_000483 record
How many amino-acids are in the protein?On which chromosome is this protein-coding gene located?
Follow the dbSNP link to examine the SNPs associated with this protein
How many SNPs?
Follow the dbSNP link to examine the SNPs associated with this protein
Describe mutation in first record. Is it synonymous?
Use the Taxonomy Browser to examine fully-sequenced primates genomes
Use the Taxonomy Browser to examine fully-sequenced prototheria genomes
Sequence Retrieval
Search for human BRCA1 in Protein database
Select longest isoform
Display the sequences in FASTA format
Save the sequence into a WORD file
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