spectrophotometry.ppt dianna
Post on 27-Apr-2015
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Spectrophotometry:
Spectroradiometers
Spectroradiometers
• designed to measure the spectral power distributions of illuminants
• they operate almost like spectrophotometers in the visible region in order to evaluate and categorize lighting for sales by the manufacturer, or for the customers to confirm the lamp they decided to purchase is within their specifications
• frequently used to calibrate LCD and CRT displays such as on laptops and HDTVs.
Components
• The light source shines onto or through the sample.
• The sample transmits or reflects light.
• The detector detects how much light was reflected from or transmitted through the sample.
• The detector then converts how much light the sample transmitted or reflected into a number.
Spectroradiometers
Spectrophotometry Applications
in Biology• Growth measurements of a bacterial culture
by spectrophotometry
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