th. elongatum was obtained with a total length of 4.63 gb ...€¦ · 03.07.2020  · integrating...

Integrating Illumina, PacBio, Bionano, and Hi-C technologies, a high-quality chromosome-level genome assembly of was obtained with a total length of 4.63 Gb (Contig N50 size of 2.15 Mb and a scaffold N50 size of 73.24 Mb) (Figure 1A and B). The successful cloning of from the Triticeae E genome and characterization of its molecular mechanism advances the knowledge on the important role of trichothecenes in pathogenesis (Figure 1C and D).

Th. elongatum

Fusarium’sFhb7

Wang H.W, Sun S.L, Ge W.Y Horizontal gene transfer of from fungus underlies Fusarium head blight resistance in wheat. 2020. Doi: 10.1126/science.aba5435.

et al. Fhb7Science

A phylogenetic analysis of the sequence revealed its distribution among Epichloë species, an endophytic fungi of temperate grasses (Figure 2A). Horizontal Gene Transfer showed genomic fragment region of with 97% identity between two genomes (Figure 2B). The DON tolerance experiment of -transgenic wheat seedlings (Figure 2C). Extract-ed ion chromatograms (EICs) (Figure 2D), and Growth inhibition assay results suggested that confers glutathione S-transferase (GST) activity to form a glutathione adduct of DON (DON-GSH) (Figure 2E and F).

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Fhb7

Fhb7

Fhb7

Figure 2A-F. confers FHB resistance by detoxifying DON.