the citric acid cycle mary gearing. problems with this pathway where does the oxaloacetate come...
Post on 02-Jan-2016
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The Citric Acid Cycle
Mary Gearing
Problems with this pathway
• Where does the oxaloacetate come from?
• How do we convert oxaloacetate to malate?
Where in the world is 1.1.1.37?
• BLAST our genome against 1.1.1.37 (H. salinarium)
Mistaken identity???
Different BLAST result
Can our organism convert pyruvate to oxaloacetate? (Enzyme 6.4.1.1)
PyruvatePyruvate
OxaloacetateOxaloacetate
6.4.1.16.4.1.1
BLASTing our genome
Both correspond to EC number 2.3.3.13 (2-isopropylmalate synthase)
So where does the oxaloacetate come from anyway?
• Aspartate metabolism
• Glycoxylate metabolism
L-aspartateL-aspartate OxaloacetateOxaloacetate2.6.1.12.6.1.1
Unanswered Questions
Oxaloacetate/malate conversion
Pyruvate/oxaloacetate conversion
We still don’t know if these processes occur in H. utahensis.
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