the nucleic acids. the history of dna…. friedrich miescher bavarie oswald avery edwin chargaff...

THE NUCLEIC ACIDS

The history of DNA….

Friedrich Miescher Bavarie Oswald Avery Edwin Chargaff Meselsohn and Stahl Hershey and Chase Watson, Crick, Wilkins and Franklin Let’s go to Tubingen and Naples

Friedrich Miescher in 1869

isolated what he called nuclein from the nuclei of pus cells

Nuclein was shown to have acidic properties, hence it became called nucleic acid

Chapter 1 …let’s go to Tubingen…

The circumstantial evidence for DNA… Griffifths Oswald Avery Chapter 3….let’s head to the US….

Transformation:Transformation:Robert Griffith (1928) Robert Griffith (1928)

DNADNA is the is the TransformeTransformer:r:Avery Avery (1944)(1944)

Hershey-Chase and DNA

The Hershey-Chase ExperimentReview of Griffifth and Hershey-Chase

There are two types of nucleic acid Deoxyribonucleic acid

(DNA) Ribonucleic acid (RNA)

DNA is found in the nucleus

with small amounts in mitochondria and chloroplasts

RNA is found throughout the cell

DNA as genetic material: The circumstantial evidence1. Present in all cells and virtually restricted to the nucleus

2. The amount of DNA in somatic cells (body cells) of any given species is constant (like the number of chromosomes)

3. The DNA content of gametes (sex cells) is half that of somatic cells. In cases of polyploidy (multiple sets of chromosomes) the DNA content increases by a proportional factor

4. The mutagenic effect of UV light peaks at 253.7nm. The peak for the absorption of UV light by DNA

7.1.1 Describe the structure of DNA, including the antiparallel strands, 3’–5’ linkages and hydrogen bonding between purines and pyrimidines

NUCLEIC ACID STRUCTURE

Nucleic acids are polynucleotides Their building blocks are nucleotides

NUCLEOTIDE STRUCTURE

PHOSPATE SUGAR

Ribose or Deoxyribose

NUCLEOTIDE

BASEPURINES PYRIMIDINES

Adenine (A)Guanine(G)

Cytocine (C)Thymine (T)Uracil (U)

Ribose is a pentose

C1

C5

C4

C3 C2

O

RIBOSE DEOXYRIBOSE

CH2OH

H

OH

C

C

OH OH

C

O

H HH

C

CH2OH

H

OH

C

C

OH H

C

O

H HH

C

Spot the difference

THE SUGAR-PHOSPHATE BACKBONE The nucleotides are all orientated

in the same direction The phosphate group joins the 3rd

carbon of one sugar to the 5th carbon of the next in line.

P

P

P

P

P

P

ADDING IN THE BASES

The bases are attached to the 1st carbon

Their order is important

It determines the genetic information of the molecule

P

P

P

P

P

P

G

C

C

A

T

T

DNA IS MADE OF TWO STRANDS OF POLYNUCLEOTIDE

P

P

P

P

P

P

C

G

G

T

A

A

P

P

P

P

P

P

G

C

C

A

T

T

Hydrogen bonds

Working out the structure of DNA…

Let’s bring in Edwin Chargaff…

Chargaff's Ratio's

Erwin Chargaff’s Data (1950-51)

Why the double helix?

Pauling's triple helix

DNA IS MADE OF TWO STRANDS OF POLYNUCLEOTIDE

The sister strands of the DNA molecule run in opposite directions (antiparallel)

They are joined by the bases Each base is paired with a specific partner:A is always paired with T G is always paired with CPurine with Pyrimidine Thus the sister strands are complementary but not identical The bases are joined by hydrogen bonds, individually weak

but collectively strong

Purines & Pyrimidines

Adenine

CytosineGuanine

Thymine

James Watson explains base pairing

Watson & Crick Base pairing

The Double Helix (1953)

© Dr Kalju Kahn USBC Chemistry and BiochemistryPublic Domain image

Wilkins & Franklin (1952): X-ray crystallography

© Norman Collection on the History of Molecular Biology in Novato, CA

DNA structure

DNA structure