trobleshooting high performance liqiud chromatography hplc reza daj 6/21/2014

TROBLESHOOTING HIGH PERFORMANCE LIQIUD CHROMATOGRAPHY HPLC

Reza Daj6/21/2014

Today’s Overview •Introduction1•Troubleshooting Approach2•Pressure Problems3•Leaks4•Component Malfunctions5•Baseline Problems6

•Peak Problems7

•Data Problems8

Introduction

TROUBLESHOOTING APPROACH

Do It Yourself

Reference Points

Common Problems

SafetyGetting the

FactsIsolating

the Sourcerepair

Strategy

Preventive Maintenanc

e

Pressure Problems

Pressure Problems

High Pressure

Low Pressure Locating a Blockage

Erratic or Cycling Pressure

Leaks

Leaks

Location the source

Pump LeaksTube fitting Leaks

Detector Leaks

Component Malfunctions

Component

Malfunctions

Electronic Problems

Injector Problems

Column Problems

Pump and Gradient

Detector Problems

BASELINE PROBLEMSNoisy Baselines1. Pump2. Gradient3. Detector4. Recorder5. Data SystemDrifting or Cycling Baseline1. Equilibration2. Late Peaks3. Mixer4. Temperature5. Electrical6. Gradient7. Cell Problem

PEAK PROBLEMS

•No Peak or Small Peaks1•Bad Peak Shape2•Extra Peak3•Negative Peak4•Incorrect Retention Time5•Poor Resolution6

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