utilization of waste bread for lactic acid fermentation · utilization of waste bread for lactic...

Utilization of waste bread for

lactic acid fermentation

Joachim Venus

Leibniz-Institut für Agrartechnik Potsdam-Bornim e.V. Max-Eyth-Allee 100, D-14469 Potsdam Fon: 0331/5699-112, E-mail: jvenus@atb-potsdam.de

BIOREFINERY FOR THE PRODUCTION OF ENERGY AND

BIO-BASED PRODUCTS

Conference Centre Torino Incontra, Torino, Italy

29-30 October, 2013

1927 Experimental farm of the Agricultural University Berlin

1933 Independent research center on agricultural mechanization

1952 Central institute of agricultural engineering of East Germany

1992 Reestablished after the reunification of Germany

Today: Leibniz Institute for Agricultural Engineering Potsdam-Bornim

- member of the Leibniz Association

History

Technology

assessment in

agricultural

systems

Technologies and processes for crop

production and livestock management

Research structure

08.11.2013 4

Biomass conversion into high-value chemical products and fuels

Biorefineries in theory would use multiple forms of biomass to produce a flexible mix of

products, including fuels, power, heat, chemicals and materials. In a biorefinery, biomass would be

converted into high-value chemical products and fuels (both gas and liquid). Byproducts and

residues, as well as some portion of the fuels produced, would be used to fuel on-site power

generation or cogeneration facilities producing heat and power.

Biotechnological

Venus, J.: Feedstocks and (Bio)Technologies for Biorefineries. – In: G.E. Zaikov, F. Pudel, G. Spychalski (Eds.),

Renewable Resources and Biotechnology for Material Applications (pp. 299-309), Nova Science Publishers, 2011

(ISBN: 978-1-61209-521-9)

08.11.2013 5

Starchy materials (cereals, industrial grade corn/potatoe starch, tapioca)

Green biomass (alfalfa, grass juice, lupine, sweet sorghum, forage rye, silage, coco juice)

Lignocellulosics (wood/straw hydrolysates, 2ndG sugars)

Residues & By-products (bagasse, oilseed cake/meal, thick juice, molasses, whey, coffee residues, waste bread,

waffle residues, algae biomass, fruit residues, meat & bone meal…)

tapioca

bagasse

waste bread

pine

coco juice

2G sugars 2G sugars

1G/2G sugars

green biomass

several residues…

lupine

cereals,

straw

sorghum

Fermentation feedstocks already tested:

silage

algae

biomass

Coffee

residues

08.11.2013 6

Table 1: Overview of chemicals that are currently

produced, or could be produced, from biomass

together with their respective market type, size of

the market, and potential biomass feedstock.

Major players involved are also given.

M.A. Abdel-

Rahman et al.

Journal of

Biotechnology

156 (2011) 286–

301

08.11.2013 7 http://www.bread4pla-life.eu

BREAD4PLA is a LIFE demonstrative European project.

It will demonstrate to the European countries the feasibility of an innovative, user

friendly and sustainable environmental solution which promotes the waste recovery in

the specific agro-food sector of the bakery industry

08.11.2013 8

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Example food waste: Bakery industry

1.Bakery waste raw materials

Collection & Characterization

3.Obtaining Polylactic Acid (PLA)

4.Material Development:

Compounding, Film

extrusion (blown film/cast)

5.Characterization of

the material developed

6. Industrial Validation:

Bakery packaging

7: INTERNATIONAL COMMUNICATION AND DISSEMINATION & EXPLOITATION MANAGEMENT

8: PROJECT MANAGEMENT.

2.Obtaining Lactic Acid (LA)

2.Obtaining Lactic Acid (LA)

9

Steps of the demonstration process

BIOSTAT®Bplus (Sartorius BBI Systems GmbH, Germany) equipped with a digital control unit DCU

This European Project is funded by LIFE+ Programme, under grant agreement LIFE 10 ENV ES 479. This ppt reflects the consortium´s opinion and the European Community is not liable for the use of the information contained herein.

ATB´s Activities in the project ACTION 2: OPTIMIZATION OF LACTIC ACID PRODUCTION FROM SELECTED WASTE BY FERMENTATION PROCESS AT PILOT PLANT LEVEL. [Demonstration action]

2.1. Up-stream processing (pre-treatment & hydrolysis of biogenic raw material/waste) 2.2. Lactic acid fermentation

2.3. Down-stream processing (separation & purification of products)

10

0

10

20

30

40

50

60

70

80

90

100

PCP-01 PBV-02 PCT-01 PCT-01plus

SF 1569 SF 1575 SF 1652 SF 1653

[g/L]

Lactate 24hLactate 48hStarch 48hZucker 48h

ACTION 2.2. Comparison of waste bread types

ACTION 2.2. Progress (in bench- and pilot-scale fermentation)

Project Meeting

Bangor (UK) 2013-07-03 11

Fermentation equipment for the scale-up of the process up to 55 (left) and 600 litres (right) of broth liquid, respectively

ACTION 2.2. Progress (in bench-scale fermentation)

12

PCB-01 (crust), enzymatic pre-hydrolysis

0

10

20

30

40

50

60

70

80

90

100

110

0 10 20 30 40 50 60 70

Lacta

te [

g/L]

time [hours]

1 L

55 L

0

5

10

15

20

25

30

0 10 20 30 40 50 60 70Sugars

[g/L]

time [hours]

1 L

55 L

This European Project is funded by LIFE+ Programme, under grant agreement LIFE 10 ENV ES 479. This ppt reflects the consortium´s opinion and the European Community is not liable for the use of the information contained herein.

ACTION 2.2. Progress (in lab-scale fermentation)

13

BIOSTAT® Bplus (Sartorius BBI Systems GmbH, Germany) equipped with a digital control unit DCU

0

20

40

60

80

100

120

0 10 20 30 40 50 60

con

cen

trat

ion

[g/

L]

time [hours]

without nutrients 15 g/L yeast extract

300 mL alfalfa juice 600 mL alfalfa juice

Bread PCP-01: Influence of (green) alfalfa juice

ACTION 2.2. Progress (in pilot-scale fermentation)

14

Comparison of Sugar Bread (PBV-02, 280312) in 600-L- and 1-L-Bioreactor

00

20

40

60

80

100

120

600-L-scale 1-L-scale

SFP 33 SF 1678

con

cen

trat

ion

[g/

L]

Lactate 24h Lactate 48h Lactate End

Starch End Sugars End

This European Project is funded by LIFE+ Programme, under grant agreement LIFE 10 ENV ES 479. This ppt reflects the consortium´s opinion and the European Community is not liable for the use of the information contained herein.

15

Fermentation

Biomass separation

Raw material storage Hydrolysis,

Pre-treatment Pre-, Microfiltration;

Sterilization of nutrient

broth & additives

Softening

Monopolar/Bipolar

Electrodialysis

Ion exchange

Decolorization

Evaporation

Pro

ce

ss

ste

ps

fo

r th

e m

an

ufa

ctu

re o

f la

cti

c a

cid

0

100

200

300

400

500

600

700

800

900

1000

0

500

1.000

1.500

2.000

2.500

End_Ferm MF NF Soft Ed_m Ed_b IC/K IC/A Decol NF_2 IC/K_2 Evap

lacta

te [

g/L]

ion

s [

mg

/L]

SFP 33, without sodium

ACTION 2.3. Progress (in DSP)

16

DSP (small scale) of Sugar Bread (PBV-02, 280312) Lactate from SFP 33

LA: Lactic Acid / (chiral) HPLC

MF/UF/NF: Micro-/Ultra-/Nanofiltration

Soft: Softening

ED: Electrodialysis

(m_monopolar; b_bipolar)

IC/K: Ion exchange/cations

IC/A: Ion exchange/anions

Decol: Decolorization

Evap: Evaporation

08.11.2013 17

Starbucks food waste transformed into bioplastics By Joe Whitworth, 23-Aug-2012

http://www.foodproductiondaily.com/Supply-Chain/Starbucks-food-waste-transformed-into-bioplastics

Starbucks’ food waste sent to a ‘food biorefinery’ has been turned into bioplastics that can

be used in packaging thanks to Hong Kong researchers.

The biorefinery changed food waste such as spent coffee grounds and stale bakery goods from

the retail chain in Hong Kong into succinic acid for making plastics. Carol S. K. Lin led the

research team who developed successful laboratory testing of a biorefinery intended to change

food waste into plastics and other everyday products. Their report on the project came at the

244th National Meeting & Exposition of the American Chemical Society.

Photo credit:

flickr/manuel I MC

Thank you very much for your attention!

With the support of:

Leibniz-Institute for Agricultural Engineering Potsdam-Bornim e.V.

Max-Eyth-Allee 100, D-14469 Potsdam, GERMANY

Fon: +49(331)5699-112

email: jvenus@atb-potsdam.de

http://de.linkedin.com/pub/joachim-venus/15/276/3b2/