yeast cellular respiration lab report (karen krmoyan) (1)
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Cellular respiration in yeast cells
Káren KrmoyanMrs. Mariam Ohanyan
IB Biology SL27 May 2016
Background: Cellular Respiration “Cellular respiration refers to the breakdown of glucose
and other respiratory substrates to make energy carrying molecules called ATP” ("Cellular Respiration").
“The role of the mitochondria in making stored chemical-bond energy available to cells by completing the breakdown of glucose to carbon dioxide” is important to understand the overall process of cell respiration (Schuster).
“Because oxygen is always in short supply anaerobic respiration dominates the metabolic activities” (“Yeast Propagation”).
Source: http://hyperphysics.phy-astr.gsu.edu/hbase/biology/imgbio/aeresp.gif
Hypothesis: cellular respiration in yeasts
If the concentration of sucrose in the solution rises, the CO2 level in yeasts will
also rise.
Variables
Controlled variables: Sucrose Room Temperature Temperature of the solution
Independent variables: Concentration of sucrose
Dependent variables: Amount of CO2 produced
Materials
Yeast (10.0 ± 0.5 g) Boiled water (2000.0 ml) Sucrose (33.0 ± 0.5 g) Vernier Oxygen Detector Vernier Carbon Dioxide Detector 2 x beakers (500.0 ml), 2 x syringes, 2 x test tubes (500.0 ml).
Procedure
1. Pour 1000.0 ml of water in each of the beakers,
2. Add 3.0 g and 30.0 g of sucrose to each beaker and solve,
3. Add 5.0 g yeast to each of the beakers and solve,
4. Using a syringe, put 5 ml of each of the solutions to different test tubes
Procedure
5. Insert the oxygen and CO2 detectors to the test tube containing 0.3% solution and collect the results using Vernier lab quest
6. Insert the oxygen and CO2 detectors to the test tube containing 3.0% solution and collect the results using Vernier lab quest
Data Processing(0.3% solution)
0 100 200 300 400 500 600 7000
2000
4000
6000
8000
10000
12000Cellular respiration in yeasts- [CO2]
per second
time/s
CO2/ppm
0 100 200 300 400 500 600 70014.814.9
1515.115.215.315.415.515.615.7
Cellular respiration in yeasts- [O2] per second
time/s
O2/ppm
Data Processing(3.0% solution)
0 100 200 300 400 500 6000
20000
40000
60000
80000
100000
120000Cellular respiration in yeasts- [CO2] per second
time/s
CO2/ppm
0 100 200 300 400 500 60015.5
15.55
15.6
15.65
15.7
15.75
15.8Cellular respiration in yeasts- [O2] per
second
time/s
O2/ppm
Evaluation & Conclusion
Overall, oxygen levels dropped, meaning that cellular respiration took place
Carbon dioxide levels, at first, rose showing that respiration took place
HOWEVER, oxygen levels fluctuated and carbon dioxide levels were unstable ~ PROBABLY due to the simultaneous photosynthesis going on [LIMITATION]
Improvements: ensure that photosynthesis does not occur
Works Cited
"Cellular Respiration." BBC Bitesize. BBC, n.d. Web. 8 May 2016. <http://www.bbc.co.uk/education/guides/z2vbb9q/revision>.
Schuster, Tom. "Yeast Respiration." Yeast Respiration. CSUN, n.d. Web. 27 May
2016. <http://www.csun.edu/scied/2-longitudinal/schuster/>."Yeast Propogation with Aerobic Respiration." Woodland Brew. Blogger, 13 Mar.
2013. Web. 27 May 2016. <http://www.woodlandbrew.com/2013/03/yeast-
propogation-with-aerobic.html>.
THANK YOU!
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