an experimental model of human absorptive enterocytes (caco-2) to assess the bioavailability of...
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An Experimental Model of Human Absorptive Enterocytes (Caco-2) to Assess
the Bioavailability of Various MetalsJonas Tallkvist
Department of Biomedical Sciences and Veterinary Public Health, Division of Pathology, Pharmacology and Toxicology, Swedish
University of Agricultural Sciences, Uppsala, Sweden
MITF 071018
Bioavailability
”The fraction of ingested metal that reaches the systemic circulation”
Factors Influencing Bioavailability
• Dose• Age• pH• Gastrointestinal contents• Nutritional status• Disease
Metals Investigated in Caco-2 Cells
V
Co
Fe
Mn
Ca
Cu
Cd
Zn
Ni
Pb
Cr
Mg
Routes of Metal Absorption
Basement membrane
Blood vessel
Mucosal/apical side
Blood/basolateral side
Enterocytes
Active Passive
The Caco-2 Cell Model
Apical chamber
Basolateral chamber Permeable filter support
Caco-2 cells
Cadmium Absorption
Zalups and Ahmad (2003) Toxcol Appl Pharm, 186, 3
Tallkvist et al., (2001) Tox Lett, 122, 171
Correlation Between DMT1 Expression and Cd Transport in Caco-2 Cells
Summary
The Caco-2 cell model provides a useful tool to assess the bioavailability of metals at various defined experimental conditions.
The Caco-2 cell model offers the possibility to gain insight into molecular mechanisms implicated in the bioavailability of metals.
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