Anatomy & Neurobiology ANA 812 Lab Session 11 Peripheral Blood Bryan Guillory Lashaundra Thedford Philip Overall John Watson Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Normal Blood (100X) Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Platelets (Thrombocytes) (1000x) Platelets Small (2-4 m) Granular Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Erythrocytes (RBCs) Normal Blood (400X) Erythrocytes -Biconcave Discs -Central Pallor Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Normal Blood Erythrocytes (1000X) Biconcave Discs Central Pallor 7-8 um in diameter Anatomy & Neurobiology Slide #43: Abnormal Human Blood (Wright-Giemsa) Anemic Blood (100X) Smaller RBCs Larger Central Pallors Variable Size and Shape Anatomy & Neurobiology Slide #43: Abnormal Human Blood (Wright-Giemsa) Anemic Blood (400X) Smaller RBCs Larger Central Pallors Variable Size and Shape Anatomy & Neurobiology Slide #43: Abnormal Human Blood (Wright-Giemsa) Anemic Blood (1000X) Various Sized RBCs Larger Central Pallor Variable Size and Shape Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Granulocytes: Neutrophils (400X) Neutrophils Granular Appearance Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Banded Neutrophils (400X) Stab Forms Nucleus Elongated Less Prominent than Segmented Forms Anatomy & Neurobiology Slide #43: Abnormal Human Blood (Wright-Giemsa) Anemic Blood (1000X) Segmented Neutrophil PMNs Lobed Nucleus w/a Fine Filament Between Most common Neutrophil Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Agranulocytes: Lymphocytes (1000x) Lymphocyte Round Nucleus Cytoplasm (blue halo) Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Agranulocytes: Monocytes (1000x) Monocyte Horseshoe, various shaped nucleus Large (14-20 m) Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Granulocytes: Eosinophil (1000x) Eosinophil Bi-lobed nucleus Granules Anatomy & Neurobiology Electron Micrograph Granulocytes: Eosinophil Lysosomal crystals Granule Nucleus Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Granulocytes: Basophil (1000x) Basophil baso because of blue-black granules when stained large granules that overlie nucleus (histamine) nucleus has smudged, indistinct chromatin pattern Anatomy & Neurobiology Slide #41: Peripheral Blood Film Human (Wright- Giemsa) 400x Monocyte