anderlei t, bürgin t, richter a, kühner ag, switzerland ...€¦ · anderlei t, bürgin t,...
TRANSCRIPT
0
10
20
30
40
50
60
100 200 250
mixing
time
[s]
shaking frequency [1/min]
mixing time 600mL TubeSpin
60 mL
120 mL
240 mL
Orbital shaken bioreactors in the field of cell cultivation
Anderlei T, Bürgin T, Richter A, Kühner AG, SwitzerlandTissot S, Monteil D, Wurm FM, EPFL, SwitzerlandBürki C, ExcellGene SA,Switzerland
Adolf Kühner AGDinkelbergstr. 1, CH-4127 Birsfelden (Basel)Switzerlandfon: +41 61 319 9393, www.kuhner.com
Kuhner Shaker Inc.295 Old County Raod, unit 11San Carlos, CA 94070, USAfon: +1 650 595 1997, [email protected]
partner of
Orbital shaking technology has been widely used in biotechnology because it offers ease-of-use, increased flexibility and reduced costs. Recently, orbital shaken bioreactors of capacities from 1mL - 2500L have been employed for cultivation of mammalian cells and are expected to become attractive alternatives to conventional stirred-tank bioreactors. Efficient mixing in shaken bioreactors is essential in order to avoid concentration gradients which can be harmful for mammalian cells. Therefore the mixing time in the different scales should be in the same range.
50-2500L scale
200-2000mL scale
10-100mL scale
shaker
orbitalshaking
20mL
100mL
10L
1mL
200L
2500L
0
20
40
60
80
100
120
140
160
180
200
30 35 40 45 50 55 60
mixing
time
[sec]
shaking frequency [1/min]
mixing time in the SB2500-X (ORBShake 2500)
2500L
2000L
1500L
1000L
500L
mix
ing
tim
e [
s]
0
20
40
60
80
100
120
140
160
180
200
40 45 50 55 60 65 70 75
mixing
time
[sec]
shaking frequency [1/min]
mixing time in the SB200-X (ORBShake 200)
200L
150L
125L
100L
mix
ing
tim
e [
s]
mix
ing
tim
e [
s]
0
10
20
30
40
50
60
100 200 250
mixing
time
[s]
shaking frequency [1/min]
mixing time 50mL TubeSpin
5 mL
10 mL
20 mL
mix
ing
tim
e [
s]
shaking diameter 50mm shaking diameter 50mm
shaking diameter 100mm
shaking diameter 50mm
shaking diameter 50mm
shaking diameter 50mm
0
10
20
30
40
50
60
100 200 250
mixing
time
[s]
shaking frequency [1/min]
mixing time 2000mL roller bottle and 5000mL Thomson flask
200 mL
400 mL
800 mL
400 mL
800 mL
1600 mL
Roller Bottle
Thomson Flask
mix
ing
tim
e [
s]
Þ shaken bioreactors have a very consistent mixing time across all
scales (less than 60 sec.)
no m
ixin
g
no m
ixin
g
no m
ixin
g
0
10
20
100 200
m
shaking frequency [1/min]
mixing time 250mL Erlenmeyer flasks with and without baffles
25 mL without baffles 25 mL with baffles
50 mL without baffles 50 mL with baffles
100 mL without baffles 100 mL with baffles
30
40
50
60
ixing
time
[s]
mix
ing
tim
e [
s]
facto
r 50'0
00