Animal Biotechnology for Improving Animal Biotechnology for Improving Productivity in Domestic Animals of Productivity in Domestic Animals of

NepalNepal

S P S P NeopaneNeopane, D , D PariyarPariyar and N A and N A GorkhaliGorkhaliNepal Agricultural Research Council (NARC)Nepal Agricultural Research Council (NARC)

OverviewOverview

BackgroundBackgroundBiotechnology Tools/Technology for Enhancing Biotechnology Tools/Technology for Enhancing Productivity of AnimalsProductivity of AnimalsBiotechnology Tools/Technology for Biotechnology Tools/Technology for Conservation and Utilization of Animals Conservation and Utilization of Animals Missing Gaps and ConstraintsMissing Gaps and ConstraintsWay ForwardWay Forward

BackgroundBackground

Importance of Biotech for Animal Importance of Biotech for Animal Productivity Productivity Used tools/tech for enhancing Used tools/tech for enhancing productivity productivity

Biotechnology Biotechnology Tools/Techniques Tools/Techniques

Biotechnology Tools for Biotechnology Tools for Improving Productivity Improving Productivity

Artificial Insemination (AI)Artificial Insemination (AI)Embryo Transfer (ET)Embryo Transfer (ET)

Artificial Insemination (AI) Artificial Insemination (AI) in Cattlein Cattle

Semen prod tech Semen prod tech established in cattleestablished in cattleAI is successful in AI is successful in cattlecattleIncreased milk prod up Increased milk prod up to 300 to 400% in cattleto 300 to 400% in cattleAv. national CR is Av. national CR is >50% (Ranges from 40 >50% (Ranges from 40 to 70%) to 70%)

Artificial Insemination (AI) Artificial Insemination (AI) in Buffaloin Buffalo

Semen prod tech Semen prod tech established in buffalo established in buffalo AI is successful in AI is successful in buffalo buffalo Increased milk prod up Increased milk prod up to 50 to 100 % in buffaloto 50 to 100 % in buffaloCR is not as high as in CR is not as high as in cattlecattle

Artificial Insemination (AI) Artificial Insemination (AI) in other speciesin other species

Semen prod tech not yet established for Semen prod tech not yet established for goat, sheep and pigsgoat, sheep and pigsAI is successful in goats and pigs and AI is successful in goats and pigs and used in a limited scaleused in a limited scaleCR is not as high as in cattleCR is not as high as in cattle

Embryo Transfer (ET)Embryo Transfer (ET)

ET established ET established successfully in cattle successfully in cattle

Embryo Transfer (ET)Embryo Transfer (ET)

Although CR is low Although CR is low Good genotypes Good genotypes borne as a result of borne as a result of ET and bulls are used ET and bulls are used for semen production for semen production

Biotechnology Tools for Biotechnology Tools for Conservation and Utilization Conservation and Utilization of Biodiversity of Biodiversity

CryoCryo--preservation preservation of genetic materials of genetic materials in different forms in different forms (semen, embryo (semen, embryo etc) etc)

Biotechnology Tools for Biotechnology Tools for Conservation and Utilization Conservation and Utilization of Biodiversity of Biodiversity

KaryotypingKaryotyping for for characteriztationcharacteriztation at at biochemical levelbiochemical level2 in cattle; 3 in buff; 2 in cattle; 3 in buff; 2 in goat; 3 in 2 in goat; 3 in sheep; 2 in pigs sheep; 2 in pigs among among indind breeds breeds karyotypedkaryotyped

Biotechnology Tools for Biotechnology Tools for Conservation and Utilization Conservation and Utilization of Biodiversity of Biodiversity

IsoIso--enzyme: In enzyme: In KhariKhari goats, protein goats, protein polymorphism madepolymorphism madeGenetic distance in Genetic distance in KhariKhari goats made and goats made and 3 diff strain identified 3 diff strain identified

Missing Gaps and Missing Gaps and ConstraintsConstraints

Missing GapsMissing Gaps

Characterization at DNA levelCharacterization at DNA levelMarker Assisted Selection Marker Assisted Selection DNA/Embryo Culture DNA/Embryo Culture Resistance genes identification for Resistance genes identification for disease and parasites disease and parasites Identification of important genes Identification of important genes

Constraints Constraints

Inadequate information on indigenous Inadequate information on indigenous breeds (for res and dev)breeds (for res and dev)--complete complete characterizationcharacterizationWeak laboratory facilitiesWeak laboratory facilitiesInadequate human resourcesInadequate human resources

Way ForwardWay Forward

Characterization of breeds at DNA level Characterization of breeds at DNA level Better understanding of Better understanding of indind breeds breeds Embryo production Embryo production Semen production in other speciesSemen production in other speciesValue addition (taste): Value addition (taste): IdenIden of genes res for of genes res for imp traits such as taste, imp traits such as taste, flavourflavour etc)etc)Genetic distance between and within Genetic distance between and within breedsbreeds

ConclusionsConclusions

AI & ET as biotech tools have increased AI & ET as biotech tools have increased prodprodKaryotypingKaryotyping as a means of characterization as a means of characterization DNA DNA characcharac limited scalelimited scale

Thank You For Your Attention

Indigenous Cattle BreedsIndigenous Cattle Breeds

Indigenous Buffalo Indigenous Buffalo BreedsBreeds

Indigenous Goat BreedsIndigenous Goat Breeds

Indigenous Sheep BreedsIndigenous Sheep Breeds

Indigenous Pig BreedsIndigenous Pig Breeds

Indigenous Poultry BreedsIndigenous Poultry Breeds

Thank You Thank You

IntroducionIntroducion

NeenaNeena AmatyaAmatya GorkhaliGorkhaliVeterinarian, IAAS RampurVeterinarian, IAAS RampurMasters in Genetics from University of Masters in Genetics from University of British Columbia, CanadaBritish Columbia, CanadaWorking in NARC since last 10 yearsWorking in NARC since last 10 years