Animal Care & Use TrainingPresented by:

Austin Fritsch

Alecia Treml

10/23/2014

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Who are your presenters? Austin Fritsch

• Austin Fritsch• Compliance Officer – IACUC & IBC

• Rules and regulations governing the use of animals in research• USDA, AWA, AALAS, AAALAC, PHS Policy, AVMA, and others

• Director – Animal Resource Center• Direct oversight of facilities and operation of both the

Schroeder and Wehr Life Science animal vivarium's10/23/2014

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Who are your presenters? Alecia Treml

• Alecia Treml• Animal Care and Facility Supervisor

• Day-to-day operation of the animal resource centers (Schroeder and Wehr)

• Coordination of animal care and use equipment and student staff

• Coordination of all ARC policies and procedures

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ACU Training – Vocabulary

• OHS – Occupational Health and Safety

• EHS – Environmental Health and Safety

• ORC – Office of Research Compliance

• ARC – Animal Resource Center

• PPE – Personal Protective Equipment

• LAA – Laboratory Animal Allergies

• SOP – Standard Operating Procedure

• IACUC – Institutional Animal Care and Use Committee

• IO – Institutional Official

• OLAW – Office of Laboratory Animal Welfare

• AWA – Animal Welfare Act

• AAALAC – Association of Assessment and Accreditation of Laboratory animal Care

• AALAS – American Association of Laboratory Animal Science 10/23/2014

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ACU Training

• Welcome to the Animal Care and Use Training Presentation• All should be here because you are working with animals

• Todays training will include• The risks working with animals

• The animal care and use program

• The animal resource center facility

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Risks working with animals - OHS training

• Occupational Health and Safety (OHS) training• Designed to protect both the institution’s

personnel and the laboratory animals

• Designed for those that have lab animal contact

• Based off of the requirements set by the Guide for the Care and Use of Laboratory Animals and Occupational Health and Safety in the Care and Use of Research Animals

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OHS Training

• Who participates?• Anyone working with or around the animals

or the ARC• Department heads

• Facility staff

• Research assistants

• Graduate staff

• Postdoctoral associates

• Investigators

• ARC staff

• Undergrad student staff 10/23/2014

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OHS Training

• What is included in the OHS training?• Tetanus vaccine should be up-to-date

• Completion of the Health Questionnaire/Risk Assessment

• If needed - a meeting with Mr. James Schabla OHS coordinator

• Each year completion of the OHS Annual Follow Up

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OHS Training – Risk Assessment

• Health Questionnaire/Risk Assessment• Copy in each folder• Will be sent in an e-mail to each participant• Questions on:

• What animals you will be working with

• Medical history

• Allergy history

• Please complete, print, sign, and bring to the ARC office (SC-561)• Please complete all information to the best of your ability

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OHS training – Laboratory Hazards

• Laboratory Hazards• It will be the responsibility of your laboratory

staff to make sure you are trained in all hazards associated with working in the lab• Sharps

• Injections

• Restraint

• Bio-hazard material

• Chemical hazards

• And others…

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OHS Training – Animal Facility Hazards

• Laboratory Animal Allergies (LAA)

• Animal bites and scratches

• Needle sticks and pokes

• Zoonosis

• Chemicals

• Hazardous agents10/23/2014

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OHS – Laboratory Animal Allergies LAAs

• Read the LAA Statement that is located in the packets

• Prolonged exposure may lead to LAAs

• 1/3 of workers have LAAs

• Inhalation is the most common route• Including eyes, skin, and respiratory system

• Sensitization usually occurs within the first 3 years10/23/2014

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OHS – Laboratory Animal Allergies LAAs

• Symptoms of allergic reaction:• Itchy, watery eyes

• Itchy, runny nose

• Chest tightness

• Wheezing

• Shortness of breath

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OHS – Laboratory Animal Allergies LAAs

• Sources of allergens from animals:• Fur

• Dander

• Feces and urine

• Saliva

• Serum

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OHS – Laboratory Animal Allergies LAAs

• Preventing LAAs• Reduce skin contact – use PPE

• gloves, lab coats, eye protection, masks, and other PPE

• Washing your hands

• No eating or drinking in animal rooms

• Wearing a respirator if needed

• Participate in training on LAAs

• Contact OHS and EHS if you need more information 10/23/2014

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OHS – Animal Bites and Scratches

• Animal bites and scratches SOP• Located by the first aid boxes SC-537 WLS-510

• Please read the SOP• Bite and scratch prevention

• Bite and scratch treatment

• No matter how small the bite or scratch please come and see the ARC staff ASAP

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OHS/EHS – Respiratory Protection Program

• Protection of Employees by• Establishing accepted practices for respirator use

• Providing guidelines for training and respirator selection

• Explaining proper storage, care, and use of respirators

• Please read through the handout in folder

• Respirator use for LAAs• FIT test required - EHS

• Please see ARC or James Schabla if a use of a respirator is needed

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OHS/EHS – Respiratory Protection Program

• Air-purifying respirators, which remove contaminants from the air

• Half mask / Dust mask

• Disposable N95 mask• Most common • Filters at least 95% of airborne

particles but is not resistant to oil.

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OHS/EHS – Respiratory Protection Program

• Air-purifying respirators, which remove contaminants from the air

• Half mask• Clean particles out of the air as you

breathe

• has disposable filters

• Filters must be replaced when they become damaged or clogged10/23/2014

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OHS/EHS – Respiratory Protection Program

• Power Air-Purifying Respirator• Uses a fan to draw air through the

filter to the user

• Easier to breathe though, but need a fully charged battery to work properly

• Use the same type of filters/cartridges as other air-purifying respirators

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OHS – LAAs

• One last note on LAAs and glove use• After handling an animal or running experiments please remove your

gloves

• LAAs can be spread through direct and indirect contact• Door handles

• Surgical equipment

• Other objects that are touched routinely

• Wash your hands after each session with an animal or group of animals

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Glove Removal and Disposal Technique

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ACU Training – Reverse Light Cycle Rooms

• Any room that is dark during normal working hours• SC – 542, 501, 511, 516B, 508

• When entering a room that is dark…• Locate a red light flashlight and enter room

• Locate the nearest red light flood light and turn it on (this will provide illumination for the entire room.

• When doing animal health checks• Use the red light flashlight to monitor animals after surgery and/or

when needing to get a closer look

• Animals can be moved into hallway if needed due to health concerns

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ACU Training – Reverse Light Cycle Rooms

• Animal Transport• Animal facility lights (outside of room and hallway) have

been lowered enough to be under the 30 foot-candle requirement• Light intensity in any procedural room/space should be

maintained at or below the 30 foot-candle recommendation

• Exception – surgery and procedures where light intensity is an experimental factor

• Surgery recovery – every attempt should be made to have the light intensity at or below 30 foot-candles. If not, drapes, towels, lab coats, covers are recommended over the animals cage while in recovery.

• Cart covers should be used when available for animal transport

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ACU Training – Whistleblower Protection

• MU Whistleblower Protection Statement• What is a whistleblower?

• Employee of Marquette University who discloses, or threatens to disclose, information to a governmental agency of the University activity that is in violation of law, rule or regulation.

• How do I report what I see?• If an employee has knowledge of information that is in vilation of any law, rule, or

regulation, the employee is encouraged to..

• Contact his/her immediate supervisor

• Visit the MU EthicsPoint website

• Or contact individuals in the protection statement10/23/2014

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ACU Training – Public Notice

• Marquette University is committed to make sure the animals well-being is #1

• No consequences will be placed onto anyone that reports a concern

• Marquette University is committed to the integrity of the research and the researcher

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ACU Training – Animal Care and Use Manual

• This manual will be e-mailed to each training participant

• Manual goes over the entire animal care and use program

• 15 chapters and includes topics such as• Principles for animal use

• Laws and Regulations

• Org. structure

• Protocol review

• Problem resolution

• Purchasing animals

• Animal health management

• And more 10/23/2014

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ACU Training – Animal Care and Use Manual

• There will be an exam – attached to your e-mail

• Open book, true false, multiple guess

• Please complete and return to the ARC office

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ACU Training - Conclusion

• Attached to an e-mail from austin.fritsch@marquette.edu• Risk assessment – complete and sign – return to Austin

• Animal Care and Use Manual

• Animal Care and Use Manual Exam – complete and return to Austin

• Please turn back to Austin completed (Risk Assessment and Exam)

• Presenting the Lab animal DVD

• Let’s go see the facility and the animals!!!!

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