annex 4 : standard requirements for the submission of ... · 2.1.3.1 short description of...

Member States seeking a financial contribution from the European Union for national programmes of eradication, control and surveillance shall submit online this application completely filled out. In case of difficulty, please contact [email protected], describe the issue and mention the version of this document:

Submission Number

1452505207258-7169

Submission Date

Monday, January 11, 2016 10:40:09

2015 1.03

Your current version of Acrobat is: 11.015

Instructions to complete the form:

1) You need to have at least the Adobe Reader version 8.1.3 or higher to fill and submit this form.

2) To verify your data entry while filling your form, you can use the “verify form” button at the top of each page.

3) When you have finished filling the form, verify that your internet connection is active, save a copy on your computer and then click on the “submit notification” button below. If the form is properly filled, the notification will be submitted to the EU server and a submission number will appear in the corresponding field. If you don't succeed to submit your programme following this procedure, check with your IT service that the security settings of your computer are compatible with this online submission procedure.

4) All programmes submitted online are kept in a central database. However only the information in the last submission is used when processing the data.

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7) For simplification purposes you are invited to submit multi-annual programmes. 8) As mentioned during the Plenary Task Force of 28/2/2014, you are invited to submit your

programmes in English.

Annex IV : Programme for the surveillance of Avian Influenza in poultry and wild birds submitted for obtaining EU cofinancing

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ANNEX 4 : Standard requirements for the submission of surveillance programmes for avian influenza in poultry and wild birds

1. Identification of the programme

Member state : SVERIGE

Disease avian influenza in poultry and wild birds

This program is multi annual : no

Request of Union co-financing from beginning of :

2016

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1.1 Contact

Name : Karolina Wall

Phone : +46 36 15 50 00

Your job type within the CA : administrator, veterinarian

Email : [email protected]

2. Description and implementation of the surveillance programme in poultry

2.1.1 Designation of the central authority in charge of supervising and coordinating the departments responsible for implementing the programme

(max. 32000 chars) :

The Swedish Board of Agriculture (SBA) is the authority under the Ministry of Enterprise and Innovation with mandate to decide about sampling, disease surveillance, eradication of epizootic diseases etc. SBA also compensates the farmer for economic losses due to decisions taken in accordance with the act on epizootic diseases. The National Veterinary Institute (SVA) is also an authority under the Ministry of Enterprise and Innovation and the National Reference Laboratory for Avian Influenza. SVA has been appointed by SBA to organize and perform the surveillance programme for avian influenza in poultry since 2002. The Department of Animal Health and Antimicrobial Strategies is responsible for the programme at SVA and handles the planning of the programme, administration, giving instructions to veterinarians involved in the sampling, sending out sampling material etc. The Department of Virology, Immunobiology and Parasitology performs the analyses. The National Food Administration (SLV, an authority under the Ministry of Enterprise and Innovation) has

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official veterinarians employed regionally. These veterinarians are responsible for the sampling at the slaughterhouses.

2.1.2 System in place for the registration of holdings


The different Swedish poultry registers are briefly presented in the table below. All registers are administered by SBA. Register Population covered EU-legislation National legislation ____________________________________________________________________________ The Swedish All commercial poultry Council Dir. Statens jordbruksverks poultry register holdings 2005/94/EC föreskrifter (2006:11) om Council Dir. registrering av anläggningar 92/66/EEC med fjäderfän The Swedish All holdings with a Council Dir. Statens jordbruksverks register capacity of 350 laying 1999/74/EC föreskrifter (2003:20) om of laying hens hen and sell eggs for Commission Dir. registrering av anläggningar consumption 2002/4/EC med värphöns Establishments All holdings with Council Dir. Statens jordbruksverks for poultry breeders for broiler 2009/158/EC föreskrifter (2010:58) om laying hen and turkey obligatorisk hälsoövervakning av fjäderfä OMNIS All poultry holdings NA Statens jordbruksverks approved within the föreskrifter (2007:78) om voluntary and frivillig och förebyggande preventive salmonella kontroll avseende salmonella control programme hos fjäderfä

2.1.3 Design (risk based surveillance or surveillance based on representative sampling taking into account criteria in Annex I of Commission Decision 2010/367/EC)


For the year 2016 Sweden has designed a surveillance programme based on the representative samling method in Annex I to Decision 2010/367/EU. Blood samples will be taken according to point 5,

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Tables 1 and 2, of this annex. For detailed information please see point 3, Tables 5.1 and 5.2. Samples will always be accompanied by referrals specially designed for the surveillance programme for avian influenza 2016. It is mandatory for the person taking the samples to fill in information on name and address of the animal owner and the farm, species sampled, identity of the flock, date of sampling, place of sampling and name of the person taking the samples. This information will be filed at SVA. All positive results will be followed up by further investigation at the holding and the Commission and the EURL will be informed of all results in accordance with Decision 2010/367/EU.

2.1.3.1 Short description of predominant poultry population and types of poultry production


The poultry production in Sweden is mainly concentrated to the south parts of the country. Laying hens The number of laying hen holdings is approximately 360. Of these, 121 have a free range holding system. 13 holdings have both indoor and free range systems. (Of the 360, these 13 holdings are "counted twice", since they have two different systems). In 2014, 3 558 326 laying hens were slaughtered in Sweden. Chicken breeders In 2015, there are 32 holdings of parent flocks of laying hens and broilers registered in the Poultry Health Control Programme. All of these are regularly controlled for different diseases by blood sampling. Turkey breeders In 2015, 3 holdings of parent flocks of turkeys are registered in the Poultry Health Control Programme. All of these are regularly controlled for different diseases by blood sampling. Fattening turkeys The number of holdings of fattening turkeys in Sweden is estimated to be around 20. In 2014, 420 280 turkeys were slaughtered in Sweden. Fattening geese and fattening ducks In Sweden, ducks and geese are mainly bred and slaughtered in the most southern parts of the country. The majority of these birds are slaughtered during October and early November. In 2014, 8 924 ducks and 16 459 geese were slaughtered in Sweden. Farmed game birds (gallinaceous) Pheasants are the most common farmed gallinaceous game bird in Sweden. All holdings with pheasant breeders should be sampled yearly. In 2014, there were 12 holdings with breeders of pheasants sampled

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by a veterinarian at the holding. Partridges have been excluded from the sampling programme since they are a small population bred under conditions with low risk for an introduction of AIV. Farmed game birds (waterfowl) All holdings with mallard breeders should be sampled yearly. In 2014, there were 5 holdings with mallard breeders sampled by a veterinarian at the holding. Ratites In 2014, 214 ratites were slaughtered in Sweden. The number om ratites in Sweden has strongly decreased during the past years. Broilers Birds from all holdings with small-scale or organic broiler production sending their birds to small-scale slaughterhouses or to the slaughterhouses for laying hens will be included in the sampling programme. These broiler holdings often have less developed biosecurity than large commercial broiler holdings. In 2014, 89 680 967 broilers were slaughtered in Sweden.

2.1.3.2 Criteria and risk factors for risk based surveillance(1)


N.A.

(1) Including maps showing target sampling sites identified as being particularly at risk for the introduction of avian influenza virus, taking into account criteria set out in point 4 of Annex I to Commission Decision 2010/367/EC.

2.2 Target populations (2)


Laying hens The number of laying hen holdings including free-range is, based on the official farm-ID numbers (PPN) of the holdings in the Swedish Poultry Register, 359 (April 2015). Of these 359, 121 have a free range holding system. 13 of the 359 holdings have both in- and outdoor holding systems. These 13 holdings are counted twice among the 359, since they have two different systems. Swedish laying hens are

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slaughtered mainly at two slaughterhouses. In 2014, 3 558 326 laying hens (from indoor and free range systems) were slaughtered in Sweden. In 2016, 60 of the indoor holdings shall be sampled at slaughter. Free range laying hens The number of free range laying hen holdings is, according to the Swedish Poultry Register, 121 (April 2015). 13 holdings have both indoor and free range holding systems. In 2016, if possible 53 of these holdings will be sampled at slaughter. Chicken breeders Samples taken in parent flocks of laying hens and broilers within the Poultry Health Control Programme will be used for the surveillance programme . Ten samples from one flock per holding (n=32 in 2015) taken from the last sampling occasion before slaughter within the programme will be analyzed. Turkey breeders Samples taken in parent flocks of turkeys within the Poultry Health Control Programme (n=3, April 2015) will be used for the surveillance. Ten samples from one flock per holding taken from the last sampling occasion before slaughter within the programme will be analyzed. Fattening turkeys All holdings that send their birds for slaughter will be sampled at the slaughterhouse. This should result in approximately 20 sampled holdings. Blood samples will be collected from ten birds per holding. Fattening ducks In 2014, 8 924 ducks were slaughtered in Sweden. All holdings that send their birds for slaughter shall be sampled at the slaughterhouse. Twenty samples per holding will be taken. If less than 20 birds are slaughtered all ducks will be sampled. Fattening geese In 2014, 16 459 geese were slaughtered in Sweden. Geese are mainly bred and slaughtered in the most southern parts of the country. The majority are slaughtered during October and early November. All Swedish holdings that send geese for slaughter shall be sampled. Twenty samples per holding will be taken at slaughter, when less than 20 birds are slaughtered all birds will be sampled. Farmed game birds (gallinaceous) Pheasants are the most common farmed gallinaceous game bird in Sweden. Partridges have been excluded from the sampling programme since they are a small population bred under conditions with low risk for an introduction of AIV. All holdings with breeders of pheasants (in 2014, 12 holdings) will be sampled by a veterinarian at the holding. Ten blood samples per holding will be taken. Farmed game birds (waterfowl) All holdings with breeders of mallards (in 2014, 5 holdings) will be sampled by a veterinarian at the holding. Twenty blood samples per holding will be taken. Ratites In 2014, 214 ostriches were slaughtered in Sweden. All holdings that send birds to slaughter should be sampled. Ten blood samples will be collected from each holding. When less than 10 birds are

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slaughtered all birds will be sampled. Broilers Birds from all holdings with small-scale or organic broiler production sending their birds to small-scale slaughterhouses or to the slaughterhouses for laying hens will be included in the sampling programme. These broiler holdings often have less developed biosecurity than large commercial broiler holdings. Blood samples will be taken from ten birds from each holding at slaughter. The Commission will be informed of any major changes in the Swedish poultry population or production that will lead to consequences for the programme. Sweden will also provide additional information on request from the Commission.

(2) including MS specific exceptional circumstances as described in Annex I point 3 of Commission Decision 2010/367/EU)

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Tota

l num

ber o

f hol

ding

s to

be

sam

pled

Num

ber o

f sam

ples

per

ho

ldin

gTo

tal n

umbe

r of t

ests

Met

hod

of la

bora

tory

ana

lysis

SE11

00

00

NA

x

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

SE12

11

1010

ELIS

A te

stx

SE21

00

00

NA

xSE

221

110

10EL

ISA

test

xSE

230

00

0N

Ax

SE31

11

1010

ELIS

A te

stx

SE32

11

1010

ELIS

A te

stx

SE33

00

00

NA

xSE

11-

33 (w

hole

of S

wed

en)

44

1040

Sam

plin

gx

Tota

l80

Add

a n

ew ro

w(a

) H

oldi

ngs o

r her

ds o

r flo

cks o

r est

ablis

hmen

ts a

s app

ropr

iate

. (b

) Re

fers

to th

e lo

catio

n of

the

hold

ing

of o

rigin

. In

case

NU

TS (N

omen

clat

ure

of T

errit

oria

l Uni

ts fo

r Sta

tistic

s) c

an n

ot b

e us

ed, r

egio

n as

def

ined

in th

e pr

ogra

mm

e by

the

Mem

ber S

tate

s is r

eque

sted

(c

) To

tal n

umbe

r of h

oldi

ngs o

f one

cat

egor

y of

pou

ltry

in c

once

rned

NU

TS 2

regi

on.

Cate

gory

: fa

rmed

gam

e bi

rds

(gal

linac

eous

)de

lete

thi

s ca

tego

ry

NU

TS (2

) (b)

Tota

l num

ber o

f hol

ding

s(c)

Tota

l num

ber o

f hol

ding

s to

be

sam

pled

Num

ber o

f sam

ples

per

ho

ldin

gTo

tal n

umbe

r of t

ests

Met

hod

of la

bora

tory

ana

lysis

SE11

11

1010

ELIS

A te

stx

SE12

44

1040

ELIS

A te

stx

SE21

00

00

NA

x

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

SE22

66

1060

ELIS

A te

stx

SE23

22

1020

ELIS

A te

stx

SE31

00

00

NA

xSE

320

00

0N

Ax

SE33

00

00

NA

xSE

11-

33 (w

hole

of S

wed

en)

1313

1013

0Sa

mpl

ing

xTo

tal

260

Add

a n

ew ro

w(a

) H

oldi

ngs o

r her

ds o

r flo

cks o

r est

ablis

hmen

ts a

s app

ropr

iate

. (b

) Re

fers

to th

e lo

catio

n of

the

hold

ing

of o

rigin

. In

case

NU

TS (N

omen

clat

ure

of T

errit

oria

l Uni

ts fo

r Sta

tistic

s) c

an n

ot b

e us

ed, r

egio

n as

def

ined

in th

e pr

ogra

mm

e by

the

Mem

ber S

tate

s is r

eque

sted

(c

) To

tal n

umbe

r of h

oldi

ngs o

f one

cat

egor

y of

pou

ltry

in c

once

rned

NU

TS 2

regi

on.

Add

a c

ateg

ory

Tota

lsTo

tal n

umbe

r of t

ests

Tota

l pou

ltry

2016

4 08

0

2.2.

2

DU

CKS

,GEE

SE A

ND

FAR

MED

GAM

E BI

RDS

(WAT

ERFO

WL

e.g.

MAL

LARD

) HO

LDIN

GS

(a) t

o be

sam

pled

.

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

Sero

logi

cal i

nves

tigat

ion

acco

rdin

g to

Ann

ex I t

o Co

mm

issi

on D

ecis

ion

2010

/367

/EU

2016

Ta

rget

s for

yea

r

Cate

gory

: fa

tteni

ng d

ucks

dele

te t

his

cate

gory

NU

TS (2

) (b)

Tota

l num

ber o

f duc

k an

d

gees

e ho

ldin

gs

Tota

l num

ber o

f duc

k an

d ge

ese

hold

ings

to b

e sa

mpl

ed

Num

ber o

f sam

ples

per

ho

ldin

gTo

tal n

umbe

r of t

ests

Met

hod

of la

bora

tory

ana

lysis

SE11

00

00

NA

xSE

120

00

0N

Ax

SE21

00

00

NA

xSE

223

320

60EL

ISA

test

xSE

230

00

0N

Ax

SE31

00

00

NA

xSE

320

00

0N

Ax

SE33

00

00

NA

xSE

11-

33 (w

hole

of S

wed

en)

33

2060

Sam

plin

gx

Tota

l12

0

Add

a n

ew ro

w(a

) H

oldi

ngs o

r her

ds o

r flo

cks o

r est

ablis

hmen

ts a

s app

ropr

iate

. (b

) Re

fers

to th

e lo

catio

n of

the

hold

ing

of o

rigin

. In

case

NU

TS (2

) cod

e ca

n no

t be

used

, reg

ion

as d

efin

ed in

the

prog

ram

me

by th

e M

embe

r Sta

te is

requ

este

d

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

Cate

gory

: fa

tteni

ng g

eese

dele

te t

his

cate

gory

NU

TS (2

) (b)

Tota

l num

ber o

f duc

k an

d

gees

e ho

ldin

gs

Tota

l num

ber o

f duc

k an

d ge

ese

hold

ings

to b

e sa

mpl

ed

Num

ber o

f sam

ples

per

ho

ldin

gTo

tal n

umbe

r of t

ests

Met

hod

of la

bora

tory

ana

lysis

SE11

00

00

NA

xSE

120

00

0N

Ax

SE21

00

00

NA

xSE

2212

1220

240

ELIS

A te

stx

SE23

11

2020

ELIS

A te

stx

SE31

00

00

NA

xSE

320

00

0N

Ax

SE33

00

00

NA

xSE

11-

33 (w

hole

of S

wed

en)

1313

2026

0Sa

mpl

ing

xTo

tal

520

Add

a n

ew ro

w(a

) H

oldi

ngs o

r her

ds o

r flo

cks o

r est

ablis

hmen

ts a

s app

ropr

iate

. (b

) Re

fers

to th

e lo

catio

n of

the

hold

ing

of o

rigin

. In

case

NU

TS (2

) cod

e ca

n no

t be

used

, reg

ion

as d

efin

ed in

the

prog

ram

me

by th

e M

embe

r Sta

te is

requ

este

d

Cate

gory

: fa

rmed

gam

e (w

ater

fow

l e.g

. mal

lard

s)de

lete

thi

s ca

tego

ry

NU

TS (2

) (b)

Tota

l num

ber o

f duc

k an

d

gees

e ho

ldin

gs

Tota

l num

ber o

f duc

k an

d ge

ese

hold

ings

to b

e sa

mpl

ed

Num

ber o

f sam

ples

per

ho

ldin

gTo

tal n

umbe

r of t

ests

Met

hod

of la

bora

tory

ana

lysis

SE11

00

00

NA

xSE

121

120

20EL

ISA

test

x

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

SE21

11

2020

ELIS

A te

stx

SE22

55

2010

0EL

ISA

test

xSE

230

00

0N

Ax

SE31

00

00

NA

xSE

320

00

0N

Ax

SE33

00

00

NA

xSE

11-

33 (W

hole

Sw

eden

)0

00

340

PCR

test

xSE

11-

33 (W

hole

Sw

eden

)0

00

10Vi

rus

isola

tion

test

xSE

11-

33 (W

hole

Sw

eden

)0

00

10ge

ne s

eque

nsin

gx

SE 1

1-33

(who

le o

f Sw

eden

)7

720

140

Sam

plin

gx

Tota

l64

0

Add

a n

ew ro

w(a

) H

oldi

ngs o

r her

ds o

r flo

cks o

r est

ablis

hmen

ts a

s app

ropr

iate

. (b

) Re

fers

to th

e lo

catio

n of

the

hold

ing

of o

rigin

. In

case

NU

TS (2

) cod

e ca

n no

t be

used

, reg

ion

as d

efin

ed in

the

prog

ram

me

by th

e M

embe

r Sta

te is

requ

este

d

Add

a c

ateg

ory

NU

TS (2

) (b)

Tota

l num

ber o

f tes

ts

Tota

l duc

ks a

nd g

eese

and

fa

rmed

gam

e bi

rds

2016

1 28

0

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

TOTA

LS fo

r Pou

ltry

(2.2

.1) +

Duc

ks a

nd G

eese

(2.2

.2)

and

farm

ed g

ame

bird

s for

yea

r :20

16

Poul

try +

D

ucks

/Gee

se /f

arm

ed g

ame

bird

s To

tal n

umbe

r of

test

sG

rand

Tot

al

5 36

0

Gra

nd T

otal

ELI

SA

2 50

0

Gra

nd T

otal

aga

r

0

Gra

nd T

otal

HI t

ests

(H5)

0

Gra

nd T

otal

HI t

ests

(H7)

0

Gra

nd T

otal

Viru

s Is

olat

ion

test

10

Gra

nd T

otal

PCR

test

34

0

Gra

nd T

otal

Oth

er te

st

10

Gra

nd T

otal

Sam

plin

gs

2 50

0

2.3

Sa

mpl

ing

proc

edur

es, s

ampl

ing

perio

ds a

nd fr

eque

ncy

of te

stin

g ta

king

into

acc

ount

crit

eria

set o

ut in

Ann

ex I o

f Co

mm

issi

on D

ecis

ion

2010

/367

/EC

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

(max

. 320

00 c

hars

) :

The

surv

eilla

nce

prog

ram

me

for a

vian

influ

enza

will

run

from

1 Ja

nuar

y to

31

Dec

embe

r 201

6. H

oldi

ngs

will

be

sam

pled

onc

e an

nual

ly a

fter

take

n in

to

cons

ider

atio

n po

ssib

le s

easo

nal f

acto

rs in

the

prod

uctio

n. L

ayin

g he

ns, b

roile

rs, f

atte

ning

turk

eys

and

ratit

es a

re s

laug

hter

ed a

nd th

eref

ore

sam

pled

rath

er

even

ly th

roug

hout

the

year

. Gee

se a

nd d

ucks

are

mai

nly

slau

ghte

red

in O

ctob

er-N

ovem

ber a

nd w

ill b

e th

eref

ore

be s

ampl

ed d

urin

g th

e au

tum

n. F

or

pare

nt fl

ocks

of l

ayin

g he

ns, b

roile

rs a

nd tu

rkey

s, s

ampl

es ta

ken

with

in th

e Po

ultr

y H

ealth

Con

trol

Pro

gram

me

will

be

used

for t

he s

urve

illan

ce

prog

ram

me.

The

se s

ampl

es a

re ta

ken

thro

ugh

out t

he y

ear.

Farm

ed fe

athe

red

gam

e is

sam

pled

at t

he h

oldi

ng, m

ainl

y du

ring

the

first

hal

f of t

he y

ear.

2.4.

La

bora

tory

test

ing

: des

crip

tion

of th

e la

bora

tory

test

s use

d an

d fo

llow

up

inve

stig

atio

ns ta

king

into

acc

ount

crit

eria

se

t out

in th

e D

iagn

ostic

Man

ual f

or a

vian

influ

enza

(Com

mon

Dec

isio

n 20

06/4

37/E

C)

Des

crip

tion

of th

e us

ed se

rolo

gica

l tes

ts :

(max

320

00 c

hars

)

In p

oultr

y flo

cks,

the

initi

al te

sts

with

in th

e sc

reen

ing

prog

ram

me

are

bloo

d sa

mpl

es, w

hich

are

ser

olog

ical

ly a

naly

sed

by a

n EL

ISA

-tes

t (ID

EXX

Influ

enza

A

Ab

test

). If

a se

rolo

gica

l tes

t is

posi

tive,

PCR

test

s ar

e pe

rfor

med

as

a fo

llow

up.

Clo

acal

and

oro

phar

ynge

al s

wab

s fr

om 6

0 bi

rds

(or a

ll bi

rds

if le

ss th

an 6

0)

in th

e po

sitiv

e ho

ldin

g ar

e th

en ta

ken.

The

two

swab

s (c

loac

al a

nd o

roph

aryn

geal

) fro

m e

ach

bird

are

poo

led

into

one

sin

gle

sam

ple,

whi

ch g

ener

ates

60

sam

ples

in to

tal.

Thes

e 60

sam

ples

are

ana

lyse

d fo

r the

det

ectio

n of

avi

an in

fluen

za v

irus

geno

me

by u

sing

an

M-g

ene

real

time

PCR

(Spa

ckm

an e

t al).

Po

sitiv

e sa

mpl

es a

re fu

rthe

r ana

lyse

d fo

r det

ectio

n an

d id

entif

icat

ion

of H

5 an

d H

7 vi

ruse

s, in

clud

ing

viru

s pa

thot

ypin

g by

am

plic

on s

eque

ncin

g (S

lom

ka

et a

l, 20

07) (

Avi

an D

isea

ses:

Vol

. 51,

No.

s1,

pp.

227

-234

). A

ll la

bora

tory

inve

stig

atio

ns w

ill b

e ca

rrie

d ou

t in

acco

rdan

ce w

ith th

e av

ian

influ

enza

dia

gnos

tic m

anua

l (Co

mm

issi

on D

ecis

ion

2006

/437

/EC)

and

poi

nt 9

of

Ann

ex I

to D

ecis

ion

2010

/367

/EU

. An

ELIS

A w

ill b

e us

ed fo

r the

ser

olog

ical

ana

lysi

s (ID

EXX

Influ

enza

A A

b te

st).

Posi

tive

resu

lts w

ill b

e co

nfirm

ed w

ith

haem

aggl

utin

atio

n in

hibi

tion

test

s (fo

r sub

type

s H

5 an

d H

7) in

acc

orda

nce

with

the

guid

elin

es.

All

labo

rato

ry te

stin

g w

ill b

e pe

rfor

med

at t

he N

atio

nal V

eter

inar

y In

stitu

te (S

VA),

Upp

sala

, Sw

eden

. All

viru

s is

olat

es w

ill b

e se

nt to

the

EU R

efer

ence

La

bora

tory

(EU

RL).

The

Swed

ish

Boar

d of

Agr

icul

ture

(SBA

) will

repo

rt to

the

Com

mis

sion

in a

ccor

danc

e w

ith A

rt 4

. of D

ecis

ion

2010

/367

/EU

.

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

3.

Des

crip

tion

and

impl

emen

tatio

n of

the

surv

eilla

nce

prog

ram

me

in w

ild b

irds

3.1.

1 D

esig

natio

n of

the

cent

ral a

utho

rity

in c

harg

e of

supe

rvis

ing

and

coor

dina

ting

the

depa

rtm

ents

resp

onsi

ble

for i

mpl

emen

ting

the

prog

ram

me

and

rele

vant

col

labo

ratin

g pa

rtne

rs (e

.g. e

pide

mio

logi

sts,

orni

thol

ogis

ts, n

atur

e bi

rd o

bser

vatio

n an

d hu

nter

or

gani

satio

ns).

(max

. 320

00 c

hars

) :

The

prog

ram

will

be

supe

rvis

ed o

f the

Sw

edis

h Bo

ard

of A

gric

ultu

re in

clo

se c

oope

ratio

n w

ith a

wor

king

gro

up o

n w

ild b

irds

and

the

Nat

iona

l Vet

erin

ary

Inst

itute

. The

wor

king

gro

up o

n w

ild b

irds

cons

ists

of o

rnith

olog

ists

as

wel

l as

epid

emio

logi

sts.

3.1.

2 D

escr

iptio

n an

d de

limita

tion

of th

e ge

ogra

phic

al a

nd a

dmin

istr

ativ

e ar

eas i

n w

hich

the

prog

ram

me

is to

be

appl

ied

max

. 320

00 c

hars

) :

The

pass

ive

surv

eilla

nce

will

be

carr

ied

out i

n th

e w

hole

are

a of

Sw

eden

.

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

3.1.

3 Es

timat

ion

of th

e lo

cal a

nd/o

r mig

rato

ry w

ildlif

e po

pula

tion

(max

. 320

00 c

hars

) :

Sw

eden

ther

e ar

e in

tota

l 490

diff

eren

t bird

spe

cies

iden

tifie

d. A

roun

d 20

0 ar

e st

ayin

g th

e w

hole

yea

r and

the

rest

are

mig

rato

ry b

irds

in m

ore

or le

ss

exte

nt. T

he m

ajor

ity o

f the

bird

s ar

e st

ayin

g in

sou

th a

nd m

iddl

e Sw

eden

and

a s

mal

ler p

art i

n th

e no

rthe

rn p

art.

In s

umm

er th

ere

are

arou

nd 5

50 m

ilion

bird

indi

vids

and

450

mill

ions

leav

e Sw

eden

in th

e en

d of

the

sum

mer

or i

n au

tum

n.

The

maj

ority

of t

he m

igra

tory

bird

s ar

rive

to S

wed

en in

Mar

s to

June

, dep

endi

ng o

n cl

imat

ic c

ondi

tions

, fro

m s

outh

and

eas

t and

a v

ery

limite

d nu

mbe

r fr

om n

orth

. Th

e Sw

edis

h or

nith

olog

ists

wor

k w

ith a

repo

rt s

yste

m c

alle

d Sv

alan

wer

e th

e m

ovem

ents

of m

igra

tory

bird

s ca

be

follo

wed

eac

h ye

ar. T

he S

wed

ish

Boar

d of

Agr

icul

ture

and

the

Nat

iona

l Vet

erin

ary

Inst

itute

are

usi

ng th

is re

port

ing

syst

em to

con

tinuo

usly

upd

ate

the

wild

bird

s si

tuat

ion.

ht

tp://

ww

w.a

rtpo

rtal

en.s

e/bi

rds/

defa

ult.a

sp

3.2

Des

ign,

crit

eria

, ris

k fa

ctor

s and

targ

et p

opul

atio

n(3)

(max

. 320

00 c

hars

) :

The

surv

eilla

nce

prog

ram

of w

ild b

irds

in S

wed

en is

des

igne

d as

a p

assi

ve p

rogr

amm

e, w

hich

incl

udes

all

grou

ps o

f wild

bird

s.

The

prog

ram

targ

ets

all w

ild b

irds

foun

d de

ad o

r mor

ibun

d. T

he b

irds

are

colle

cted

from

the

who

le a

rea

of S

wed

en a

nd s

ent t

o th

e N

atio

nal V

eter

inar

y In

stitu

te in

Upp

sala

for s

ampl

ing.

Sin

ce th

ere

have

bee

n ve

ry fe

w in

cide

nts

of in

fluen

za in

the

coun

try,

no

spec

ial r

isk

fact

ors

have

bee

n de

fined

; all

foun

d bi

rds

are

incl

uded

in th

e pr

ogra

mm

e.

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

(3)

Are

as a

t ri

sk (

wet

land

s in

par

ticu

lar

whe

re l

inks

wit

h hi

gh d

ensi

ty p

oult

ry p

opul

atio

ns),

pre

viou

s po

siti

ve f

indi

ngs

as r

efer

red

to i

n po

int

2 of

Par

t 1

of A

nnex

II t

o Co

mm

issi

on D

ecis

ion

2010

/367

/EC

shou

ld b

e ta

ken

into

acc

ount

and

if

poss

ible

com

plem

ente

d by

a m

ap.

3.2.

1 W

ILD

BIR

DS

focu

ssed

on

targ

et sp

ecie

s

Inve

stig

atio

ns a

ccor

ding

to th

e su

rvei

llanc

e pr

ogra

mm

e se

t out

in c

onfo

rmity

with

Par

t 2 o

f Ann

ex II

to D

ecis

ion

2010

/367

/EC

2016

Ta

rget

s for

yea

r

NU

TS (2

) cod

e/re

gion

(a)

Tota

l num

ber o

f bird

s to

be

sam

pled

Estim

ated

tota

l num

ber o

f sa

mpl

es to

be

take

n fo

r pa

ssive

sur

veilla

nce

Type

of t

est

Num

ber o

f tes

ts

SE 1

1-33

(who

le te

rrito

ry o

f Sw

eden

)50

060

0PC

R te

st60

0x

SE 1

1-33

(who

le te

rrito

ry o

f Sw

eden

)0

0Vi

rus

isola

tion

test

50x

SE 1

1-33

(who

le te

rrito

ry o

f Sw

eden

)0

0G

ene

sequ

ensin

g50

xTo

tal

Add

a n

ew ro

w

(a)

Refe

rs to

the

plac

e of

col

lect

ion

of b

irds/

sam

ples

. In

case

NU

TS 2

(Nom

encl

atur

e of

Ter

ritor

ial U

nits

for S

tatis

tics)

can

not

be

used

, reg

ion

as d

efin

ed in

the

prog

ram

me

by

the

Mem

ber S

tate

is re

ques

ted.

Ple

ase

fill-i

n th

ese

valu

es d

irect

ly in

the

field

.

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

Tota

l num

ber o

f tes

ts

Tota

l num

ber o

f tes

ts

700

Tota

l Viru

s is

olat

ion

test

s50

Tota

l PCR

test

s60

0

Tota

l Oth

er te

sts

50

3.3

Sam

plin

g pr

oced

ures

and

sam

plin

g pe

riods

max

320

00 c

hars

:

Wild

bird

s fo

und

dead

or m

orib

und

are

send

by

post

by

the

finde

r in

exis

ting

rout

ines

to th

e N

atio

nal

Vete

rinar

y In

stitu

te in

Upp

sala

for s

ampl

ing

and

anal

ysis

. In

case

of e

nhan

ced

inve

stig

atio

ns, i

n fo

reha

nd c

ontr

acte

d or

gani

satio

ns w

ill d

o lo

cal e

xam

inat

ions

for d

ead

or m

orib

und

wild

bird

s fo

r fur

ther

ex

amin

atio

n an

d sa

mpl

ing

at N

RL.

3.4

Labo

rato

ry te

stin

g : d

escr

iptio

n of

the

labo

rato

ry te

sts u

sed

taki

ng in

to a

ccou

nt c

riter

ia se

t out

in th

e D

iagn

ostic

M

anua

l for

avi

an in

fluen

za (C

omm

issi

on D

ecis

ion

2006

/437

/EC)

max

320

00 c

hars

:

From

dea

d bi

rds,

two

swab

sam

ples

(clo

acal

and

trac

heal

) are

col

lect

ed. T

he s

ampl

es a

re a

naly

sed

for t

he d

etec

tion

of a

vian

inlu

enza

viru

s ge

nom

e by

us

ing

an M

-gen

e re

altim

e PC

R (S

pack

man

et a

l). P

ositi

ve s

ampl

es a

re fu

rthe

r ana

lyse

d fo

r det

ectio

n a

nd id

entif

icat

ion

of H

5 an

d H

7 vi

ruse

s, in

clud

ing

viru

s pa

thot

ypin

g by

am

plic

on s

eque

ncin

g (S

lom

ka e

t al).

If v

irus

geno

me

is d

etec

ted

viru

s is

olat

ion

and

furt

her s

ubty

ping

are

per

form

ed. T

he v

iruse

s is

olat

ed

are

sent

to C

RL.

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

4.

Des

crip

tion

of th

e ep

idem

iolo

gica

l situ

atio

n of

the

dise

ase

in p

oultr

y du

ring

the

last

five

yea

rsm

ax 3

2000

cha

rs :

With

in th

e EU

sur

veill

ance

pro

gram

me

for A

IV, t

he fo

llow

ing

num

bers

of h

oldi

ngs

have

bee

n te

sted

dur

ing

the

year

s 20

07-2

014:

2

007

2

008

2

009

2

010

2011

2012

2013

2014

La

ying

hen

s

60

6

5

61

6

2

61

52

44

5

8 Fr

ee ra

nge

layi

ng h

ens

n/a

n/

a

n

/a

n/a

30

27

1

6

23

Turk

eys

23

23

1

7

21

22

1

9

26

16

Duc

ks

3

8

3

4

6

3

1

3

Gee

se

16

3

0

13

1

1

2

0

20

13

9

Br

oile

rs¹

1

7

28

2

7

24

39

3

4

2

6

12

Ratit

es

1

0

10

6

4

5

3

2

3

Bree

ding

hen

s (p

aren

ts)

4

0

42

3

3

34

36

36

36

3

2 Br

eedi

ng tu

rkey

s (p

aren

ts)

4

2

4

3

3

3

3

2

Gam

e bi

rds

(mal

lard

s)

7

6

6

7

9

7

7

5

Gam

e bi

rds

(phe

asan

ts)

2

3

23

2

0

17

15

16

1

6

12

Back

yard

floc

ks

0

0

6

0

0

0

0

0 (¹

Smal

l-sca

le p

rodu

ctio

n)

Befo

re 2

006

AIV

had

nev

er b

een

dete

cted

in S

wed

ish

poul

try.

In M

arch

200

6 hi

ghly

pat

hoge

nic

avia

n in

fluen

za (H

PAIV

) viru

s su

btyp

e H

5 w

as is

olat

ed in

sa

mpl

es, t

aken

due

to fi

ndin

gs in

the

wild

bird

pop

ulat

ion,

from

a m

alla

rd o

n a

Swed

ish

gam

e fa

rm. T

here

wer

e no

clin

ical

sig

ns in

the

flock

. Sin

ce 2

006

no

findi

ngs

of lo

w p

atho

geni

c H

5 or

H7

or h

igh

path

ogen

ic s

trai

ns o

f AIV

hav

e be

en d

one

in S

wed

ish

poul

try

flock

s. S

ever

al in

vest

igat

ions

hav

e be

en

perf

orm

ed, t

houg

h, d

ue to

pos

itive

ser

olog

ical

find

ings

with

in th

e su

rvei

llanc

e pr

ogra

mm

e.

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

In 2

010,

ant

ibod

ies

agai

nst A

IV s

ubty

pe H

5 w

ere

foun

d in

four

hol

ding

s w

ith m

alla

rd b

reed

ers.

The

hol

ding

s w

ere

furt

her i

nves

tigat

ed b

y cl

oaca

l and

or

opha

ryng

eal s

wab

sam

plin

g. N

o A

IV c

ould

be

dete

cted

by

PCR-

anal

ysis

of s

wab

sam

ples

from

two

of th

e ho

ldin

gs. I

n sa

mpl

es fr

om t

he o

ther

two

hold

ings

influ

enza

A v

irus

geno

me

was

det

ecte

d in

the

M-g

ene

PCR.

Fur

ther

ana

lyse

s of

thes

e sa

mpl

es in

clud

ing

PCR

for H

5 an

d H

7 an

d vi

rusi

sola

tion

atte

mpt

s w

ere

nega

tive.

In

201

1, a

ntib

odie

s ag

ains

t AIV

sub

type

H5

wer

e fo

und

in tw

o ho

ldin

gs w

ith m

alla

rd b

reed

ers.

The

hol

ding

s w

ere

furt

her i

nves

tigat

ed b

y cl

oaca

l and

or

opha

ryng

eal s

wab

sam

plin

g. N

o A

IV c

ould

be

dete

cted

by

PCR-

anal

ysis

of s

wab

sam

ples

from

the

two

hold

ings

In

201

2, n

o an

tibod

ies

agai

nst A

IV s

ubty

pe H

5 or

H7

wer

e fo

und.

In

201

3, a

ntib

odie

s ag

ains

t AIV

sub

type

H5

wer

e fo

und

in o

ne h

oldi

ng w

ith m

alla

rd b

reed

ers.

The

hol

ding

was

furt

her i

nves

tigat

ed b

y cl

oaca

l and

or

opha

ryng

eal s

wab

sam

plin

g. N

o A

IV H

5 or

H7

coul

d be

det

ecte

d by

PCR

-ana

lysi

s of

sw

ab s

ampl

es fr

om th

e ho

ldin

g.

In 2

014,

no

antib

odie

s ag

ains

t AIV

sub

type

H5

or H

7 w

ere

foun

d.

5.

Des

crip

tion

of th

e ep

idem

iolo

gica

l situ

atio

n of

the

dise

ase

in w

ild b

irds d

urin

g th

e la

st fi

ve y

ears

(max

. 320

00 c

hars

) :

Unt

il sp

ring

2006

hig

hly

pato

geni

c av

ian

influ

enza

viru

s (H

PAI)

has

neve

r bee

n de

tect

ed in

wild

bird

s in

Sw

eden

. Pre

viou

s su

rvei

llanc

e si

nce

autu

mn

2002

at

the

bird

con

serv

atio

n st

atio

n at

Ott

enby

, Öla

nd, h

as d

emon

stra

ted

that

HPA

I was

not

pre

sent

dur

ing

that

per

iod.

Mal

lard

s ar

e th

e sp

ecie

s th

at m

ost

sam

ples

com

e fr

om.

At t

he N

atio

nal V

eter

inar

y In

stitu

te s

urve

illan

ce in

all

dise

ased

or d

ead

bird

spe

cies

is o

ngoi

ng s

ince

a lo

ng p

erio

d (e

xcee

ding

the

last

five

yea

rs).

In b

irds

foun

d de

ad o

r dis

ease

d in

the

wild

nec

rops

y is

per

form

ed a

nd if

cha

nges

are

obs

erve

d th

at le

ad to

a s

uspi

cion

con

cern

ing

AI o

r ND

furt

her t

ests

are

pe

rfor

med

. At t

he m

omen

t all

bird

s fo

und

dead

are

test

ed fo

r AI.

HPA

I has

bee

n de

tect

ed in

65

wild

bird

s du

ring

sprin

g 20

06. L

ow p

atho

geni

c av

ian

influ

enza

viru

s (L

PAI)

has

been

foun

d in

Mal

lard

s w

ith a

qui

te h

igh

prev

alen

ce b

ut h

as a

lso

been

foun

d in

sev

eral

oth

er s

peci

es li

ke fo

r exa

mpl

e Bl

ack

head

ed g

ull,

Mew

gul

l, be

an g

oose

and

Tea

l. D

urin

g 20

09 a

nd 2

010

no

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

case

of H

PAI h

as b

een

dete

cted

in S

wed

en in

wild

bird

s. L

PAI h

as b

een

foun

d in

69

of th

e 3

863

sam

pled

bird

s, m

ostly

in M

alla

rds

and

in a

few

Eur

asia

n w

igeo

ns in

the

activ

e su

rvei

llanc

e du

ring

the

autu

mn

2009

. Dur

ing

2011

and

201

2 ne

ither

HPA

I nor

LPA

I is

dete

cted

. Dur

ing

2013

thre

e ca

ses

of L

PAI h

as

been

foun

d on

mal

lard

s at

the

sam

e pl

ace

and

time.

Dur

ing

2014

no

case

of H

PAI h

as b

een

dete

cted

in w

ild b

irds.

LPA

I ???

???

6.

Mea

sure

s in

plac

e as

rega

rds t

he n

otifi

catio

n of

the

dise

ase

(max

. 320

00 c

hars

) :

Avi

an in

fluen

za is

incl

uded

in th

e Sw

edis

h ac

t on

epiz

ootic

dis

ease

s (E

pizo

otila

gen,

SFS

199

9:65

7). A

ccor

ding

to th

is a

ct b

oth

anim

al o

wne

rs a

nd

vete

rinar

ians

are

obl

iged

to re

port

sus

pect

cas

es o

f avi

an in

fluen

za. T

his

appl

ies

to b

oth

clin

ical

and

labo

rato

ry s

uspi

cion

s. If

AI i

s su

spec

ted

or c

onfir

med

on

a fa

rm, m

easu

res

will

be

take

n ac

cord

ing

to C

ounc

il D

irect

ive

2005

/94/

EC a

nd C

omm

issi

on D

ecis

ion

2010

/367

/EU

7.

Cost

s

7.1

Det

aile

d an

alys

is o

f the

cos

ts

7.1.

1 P

oultr

y in

clud

ing

duck

s, ge

ese

and

farm

ed g

ame

bird

s

(max

. 320

00 c

hars

) :

Adm

inis

trat

ion

(pla

nnin

g, a

dmin

istr

atio

n of

the

prog

ram

me,

info

rmat

ion,

repo

rtin

g, fo

llow

up

etc)

(190

tim

mar

x 1

130

SEK=

214

700

SEK)

2

15 0

00 S

EK

Sam

plin

g (c

olle

ctin

g sa

mpl

es a

t sla

ught

erho

uses

): Be

räkn

ad to

tal å

tgån

g ca

.90

timm

ar: 0

,5 ti

mm

e fö

r pro

vtag

ning

10

prov

er (h

önsf

ågla

r, st

ruts

), 0,

75

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

timm

e fö

r pro

vtag

ning

20

prov

er (g

ås, a

nka)

. Byg

ger p

å an

taga

ndet

att

113

(60

inne

- och

53

uteg

åend

e) v

ärph

önsf

lock

ar p

rovt

as, s

amtli

ga p

å sl

akte

ri.

Mes

t tro

ligt k

omm

er v

i doc

k in

te k

omm

a up

p i d

etta

ant

al a

vsee

nde

uteg

åend

e. S

anno

likt a

rvod

e 20

16, e

nlig

t SLV

: 716

SEK

/tim

me.

71

6 SE

K x

90 t

= 64

440

SEK

. Avr

undn

ing

någo

t upp

åt:

64

500

SEK

Sam

plin

g (c

olle

ctin

g sa

mpl

es a

t hol

ding

s):

Prov

tagn

ing

vilth

ägn:

I an

söka

n fö

r 201

5: b

eräk

nat 2

625

SEK

/häg

n. F

ör 2

016:

upp

skat

tnin

g: ti

mko

stna

d up

präk

nad

3%, g

er

2 70

4 SE

K/hä

gn. B

eräk

nat p

å 13

häg

n fa

san

+ 7

hägn

grä

sand

= 2

0 vi

ltfåg

elhä

gn. P

rovt

as à

2 7

04 S

EK/h

ägn

= 54

080

SEK

. Avr

unda

s:

5

4 10

0 SE

K

Sam

plin

g eq

uipm

ent (

tube

s, p

last

ic b

ags,

env

elop

es e

tc) a

nd tr

ansp

ort c

osts

1

6 00

0 SE

K

Sero

logy

: To

tal n

umbe

r of t

ests

:

2

500

EL

ISA

cos

t per

sam

ple

83

SEK

(the

cos

t will

als

o in

clud

e ne

cess

ary

conf

irmat

ion

with

HI-t

est)

__

____

____

____

____

____

____

____

____

____

____

____

____

____

____

____

__

Tota

l ser

olog

y:

20

7 50

0

SE

K

PCR

and

Viru

siso

latio

n:

Estim

ated

no

of s

erop

ositi

ve h

oldi

ngs

= 3

H

oldi

ng n

o. 1

with

two

poul

try

cate

gorie

s

Hol

ding

s no

s 2

and

3 w

ith o

ne p

oultr

y ca

tego

ry o

f whi

ch h

oldi

ng n

o 3

anal

ysed

out

of n

orm

al w

orki

ng h

ours

(+ 5

0% c

ost/

test

)

N

o of

sam

ples

N

o of

hol

ding

s

N

o of

test

s

Cos

t/te

st

La

bora

tory

cos

ts

M-g

ene

PCR

hold

ing

no. 1

2

40

1

1

20

54

0

64

800

SEK

M-g

ene

PCR

hold

ing

no. 2

1

20

1

60

5

40

3

2 40

0 SE

K

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

M-g

ene

PCR

hold

ing

no. 3

1

20

1

60

8

10

4

8 60

0 SE

K H

5-PC

R

2

5

2

5

0

360

18

000

SEK

H7-

PCR

25

2

50

3

60

1

8 00

0 SE

K Se

quen

cing

5

2

10

13

80

1

3 80

0 SE

K Vi

rusi

sola

tion

5

2

10

1

000

1

0 00

0 SE

K __

____

____

____

____

____

____

____

____

____

____

____

____

____

____

____

____

____

____

____

____

____

____

____

____

____

____

___

Tota

l PCR

and

viru

siso

latio

n:

20

5 60

0 SE

K 7.

1.2

W

ild b

irds

(max

. 320

00 c

hars

) :

Del

iver

y (c

olle

ctio

n, tr

ansp

ort a

nd s

ampl

ing)

of w

ild b

irds

500

500

6

05 S

EK

302

500

M

-gen

e re

altim

e PC

R (p

oole

d)

5

00

5

00

540

SEK

27

0 00

0 H

5/H

7-PC

R (c

onfir

mat

ory

test

)

10

0

1

00

360

SEK

36 0

00

Gen

e se

quen

sing

5

0

50

1 3

80 S

EK

69

000

Viru

s is

olat

ion

5

0

50

1

000

SEK

5

0 00

0

--

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

----

--

Tota

l sam

plin

g, P

CR a

nd v

irusi

sola

tion

727

500

SE

K

This

app

licat

ion

does

not

cov

er th

e co

sts

follo

win

g an

eve

ntua

l and

ext

ensi

ve A

I out

brea

k a

mon

g w

ild b

irds

that

may

requ

ire e

xpan

ded

activ

e sa

mpl

ing

and

anal

ysis

of d

ead

and

sick

bird

s in

spe

cific

are

as in

acc

orda

nce

with

Com

mis

sion

Dec

isio

n 20

10/3

67/E

U.

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

7.2

Sum

mar

y of

the

annu

al c

osts

:

7.2.

1

Poul

try

surv

eilla

nce

incl

udin

g du

cks,

gees

e an

d fa

rmed

gam

e bi

rds

Det

aile

d an

alys

is o

f the

cos

t of t

he p

rogr

amm

e - p

oultr

y

2016

Ta

rget

s for

yea

r

Labo

rato

ry te

stin

g Met

hods

of l

abor

ator

y an

alys

isN

umbe

r of t

ests

Indi

cativ

e un

itary

test

cos

t (pe

r m

etho

d) in

€ (*

)To

tal c

ost (

€)

ELIS

A te

st25

003.

2681

50

agar

gel

imm

une

diffu

sion

test

01.

80

HI-T

est f

or H

5 (s

pecif

y nu

mbe

r of t

ests

for H

5)0

9.64

0

HI-T

est f

or H

7 (s

pecif

y nu

mbe

r of t

ests

for H

7)0

9.64

0

Viru

s iso

latio

n te

st10

37.8

837

8.8

PCR

test

340

19.7

467

11.6

Gen

e se

quen

sing

1015

015

00

Sam

plin

g

Num

ber o

f sam

ples

Uni

tary

cos

t in

€ (*

)To

tal c

ost (

€)

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

Sam

ples

2 50

06.

0915

225

Oth

er m

easu

res

Num

ber o

f sam

ples

Uni

tary

cos

t in

€To

tal c

ost (

€)

N.A

.0

00

xA

dd a

new

row

Tota

l pou

ltry,

duc

k, g

eese

, far

med

gam

e bi

rds

Te

stin

g +

Sam

plin

g +

Oth

er m

easu

res

31 9

65,4

0 €

(*)

as p

er c

ofin

anci

ng d

ecis

ion

for 2

014

prog

ram

mes

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

7.2.

2

Wild

bird

surv

eilla

nce

Det

ail a

naly

sis o

f the

cos

t of t

he p

rogr

amm

e - w

ild b

irds

2016

Ta

rget

s for

yea

r

Labo

rato

ry te

stin

g Met

hods

of l

abor

ator

y an

alys

isN

umbe

r of t

ests

Uni

tary

test

cos

t (pe

r met

hod)

in €

(*)

Tota

l cos

t (€)

Viru

s iso

latio

n te

st50

37.8

818

94

PCR

test

600

19.7

411

844

Oth

er c

ost

5015

075

00

Del

iver

y of

wild

ani

mal

s

No

of w

ild b

irds

Elig

ible

cos

t in

€ (*

)To

tal c

ost (

€)

Del

ivery

of w

ild a

nim

als

500

1050

00

Oth

er m

easu

res

Num

ber

Uni

tary

cos

t in

€To

tal c

ost (

€)

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

N.A

.0

00

xA

dd a

new

row

Tota

l wild

bird

s T

estin

g +

Deliv

ery

+ O

ther

mea

sure

s26

238

,00

€

(*)

as p

er c

ofin

anci

ng d

ecis

ion

for 2

014

prog

ram

mes

TOTA

LS fo

r Pou

ltry,

duc

k, g

eese

, far

med

gam

e bi

rds (

7.2.

1) +

WIL

D B

IRD

S (7

.2.2

) fo

r yea

r :20

16

Tota

l Cos

t

Gra

nd T

otal

Pou

ltry,

Duc

ks/G

eese

/Far

med

gam

e bi

rds

+ W

ILD

BIRD

S 58

203,

4

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

C. F

inan

cial

info

rmat

ion

1. I

dent

ifica

tion

of t

he im

plem

entin

g en

titie

s -

fin

anci

al c

ircu

its/f

low

s Id

entif

y an

d de

scribe

th

e en

titie

s w

hich

w

ill be

in

ch

arge

of

im

plem

entin

g th

e el

igib

le m

easu

res

plan

ned

in th

is

prog

ram

me

whi

ch c

osts

will

con

stitu

te t

he r

eim

burs

men

t/pa

ymen

t cl

aim

to

the

EU.

Des

crib

e th

e fin

anci

al f

low

s/ci

rcui

ts

follo

wed

. Ea

ch o

f th

e fo

llow

ing

para

grap

hs (

from

a t

o e)

sha

ll be

fill

ed o

ut if

EU

cof

inan

cing

is r

eque

sted

for

the

rel

ated

mea

sure

.

a) I

mpl

emen

ting

entit

ies

- s

amp

ling:

who

per

form

the

off

icia

l sam

plin

g? W

ho p

ays?

(e

.g.

auth

oris

ed p

riva

te v

ets

perf

orm

the

sam

plin

g an

d ar

e pa

id b

y th

e re

gion

al v

eter

inar

y se

rvic

es (

stat

e bu

dget

);

sam

plin

g eq

uipm

ent

is p

rovi

ded

by t

he p

riva

te l

abor

ator

y te

stin

g th

e sa

mpl

es w

hich

inc

lude

s th

e pr

ice

in t

he i

nvoi

ce

whi

ch is

pai

d by

the

loca

l sta

te v

eter

inar

y se

rvic

es (

stat

e bu

dget

))

(max

. 320

00 c

hars

) :

The

offic

ial s

ampl

ing

at h

oldi

ngs

rega

rdin

g po

ultr

y is

per

form

ed b

y au

thor

ised

priv

ate

vets

and

offi

cial

vet

s em

ploy

ed b

y SB

A. T

he s

ampl

ing

is p

aid

by S

BA

(sta

te b

udge

t) a

nd th

e sa

mpl

ing

equi

pmen

t is

prov

ided

by

the

Nat

iona

l Vet

erin

ary

Inst

itute

. Sam

plin

g at

the

slau

ghte

rhou

ses

are

perf

orm

ed b

y of

ficia

l ve

ts e

mpl

oyed

by

NFA

. The

sam

plin

g co

st is

pai

d by

SBA

(sta

te b

udge

t) a

nd th

e sa

mpl

ing

equi

pmen

t is

prov

ided

by

the

Nat

iona

l Vet

erin

ary

Inst

itute

. The

sa

mpl

ing

of w

ild b

irds

is p

erfo

rmed

by

vets

at t

he N

atio

nal V

eter

inar

y In

stitu

te, a

fter

the

bird

s ha

s be

en d

eliv

ered

ther

e. T

his

sam

plin

g is

pai

d by

sta

te

budg

et.

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

b) I

mpl

emen

ting

entit

ies

- t

esti

ng:

who

per

form

s th

e te

stin

g of

the

off

icia

l sam

ples

? W

ho p

ays?

(e

.g.

regi

onal

pub

lic la

bora

tories

per

form

the

tes

ting

of o

ffic

ial s

ampl

es a

nd c

osts

rel

ated

to

this

tes

ting

are

entir

ely

paid

by

the

sta

te b

udge

t)

(max

. 320

00 c

hars

) :

The

test

ing

is p

erfo

rmed

by

the

Nat

iona

l Vet

erin

ary

Inst

itute

and

is p

aid

for b

y SB

A.

c) I

mpl

emen

ting

entit

ies

- c

omp

ensa

tion

: w

ho p

erfo

rms

the

com

pens

atio

n? W

ho p

ays?

(e

.g.

com

pens

atio

n is

pai

d by

the

cen

tral

leve

l of

the

stat

e ve

terina

ry s

ervi

ces,

or

com

pens

atio

n is

pai

d by

an

insu

ranc

e fu

nd f

ed b

y co

mpu

lsor

y fa

rmer

s co

ntribu

tion)

(max

. 320

00 c

hars

) :

No

com

pens

atio

n fo

r sam

plin

g is

pai

d to

the

farm

ers.

The

test

ing

is c

ompu

lsor

y fo

r tho

se c

hose

n fo

r sam

plin

g.

d) I

mpl

emen

ting

entit

ies

- v

acci

nat

ion

: w

ho p

rovi

des

the

vacc

ine

and

who

per

form

s th

e va

ccin

atio

n? W

ho p

ays

the

vacc

ine?

Who

pay

s th

e va

ccin

ator

? (e

.g.

farm

ers

buy

thei

r va

ccin

e to

the

priva

te v

ets,

sen

d th

e pa

id i

nvoi

ces

to t

he l

ocal

sta

te v

eter

inar

y se

rvic

es w

hich

re

imbu

rse

the

farm

ers

of t

he f

ull a

mou

nt a

nd t

he v

acci

nato

r is

pai

d by

the

reg

iona

l sta

te v

eter

inar

y se

rvic

es)

(max

. 320

00 c

hars

) :

No

vacc

inat

ion

is p

erm

itted

i Sw

eden

.

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

e) I

mpl

emen

ting

entit

ies

- o

ther

ess

enti

al m

easu

res:

who

im

plem

ent

this

mea

sure

? W

ho p

rovi

de t

he e

quip

men

t/se

rvic

e? W

ho p

ays?

(max

. 320

00 c

hars

) :

N.A

2 Co-

finan

cing

rat

e (

see

prov

isio

ns o

f ap

plic

able

Wor

k Pr

ogra

mm

e)

The

max

imum

co-

fina

ncin

g ra

te i

s in

gen

eral

fix

ed a

t 50

%. H

owev

er b

ased

on

prov

isio

ns o

f A

rtic

le 5

.2 a

nd 5

.3 o

f th

e Re

gula

tion

(EU

) N

o 65

2/20

14,

we

requ

est

that

the

co-

fina

ncin

g ra

te f

or t

he r

eim

burs

emen

t of

the

eli

gibl

e co

sts

wou

ld b

e in

crea

sed:

Up

to 7

5% fo

r the

mea

sure

s det

aile

d be

low

Up

to 1

00%

for t

he m

easu

res d

etai

led

belo

w

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

3. S

ourc

e of

fun

ding

of

elig

ible

mea

sure

s

All

elig

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mea

sure

s fo

r w

hich

cof

inan

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is r

eque

sted

and

rei

mbu

rsm

ent

will

be

clai

med

are

fin

ance

d by

pub

lic f

unds

.

yes

no

of 3

9

AN

NEX

4 :

Sta

ndar

d re

quirem

ents

for

the

sub

mis

sion

of

surv

eilla

nce

prog

ram

mes

for

avi

an

influ

enza

in p

oultr

y an

d w

ild b

irds

Atta

chm

ents

IMP

OR

TAN

T :

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form

at li

sted

her

e : j

pg, j

peg,

tiff,

tif,

xls,

xls

x, d

oc, d

ocx,

ppt

, ppt

x, b

mp,

pna

, pdf

.

3) T

he to

tal f

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a-z

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