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Member States seeking a financial contribution from the European Union for national programmes of eradication, control and surveillance shall submit online this application completely filled out. In case of difficulty, please contact [email protected], describe the issue and mention the version of this document:
Submission Number
1441354646112-6665
Submission Date
Friday, September 04, 2015 11:17:23
2015 1.03
Your current version of Acrobat is: 11.015
Instructions to complete the form:
1) You need to have at least the Adobe Reader version 8.1.3 or higher to fill and submit this form.
2) To verify your data entry while filling your form, you can use the “verify form” button at the top of each page.
3) When you have finished filling the form, verify that your internet connection is active, save a copy on your computer and then click on the “submit notification” button below. If the form is properly filled, the notification will be submitted to the EU server and a submission number will appear in the corresponding field. If you don't succeed to submit your programme following this procedure, check with your IT service that the security settings of your computer are compatible with this online submission procedure.
4) All programmes submitted online are kept in a central database. However only the information in the last submission is used when processing the data.
5) IMPORTANT: Once you have received the submission number, save the form on your computer for your records.
6) If the form is not properly filled in, an alert box will appear indicating the number of incorrect fields. Please check your form again, complete it and re-submit it according to steps 3). Should you still have difficulties, please contact [email protected].
7) For simplification purposes you are invited to submit multi-annual programmes. 8) As mentioned during the Plenary Task Force of 28/2/2014, you are invited to submit your
programmes in English.
Annex IV : Programme for the surveillance of Avian Influenza in poultry and wild birds submitted for obtaining EU cofinancing
Page 2 of 32
ANNEX 4 : Standard requirements for the submission of surveillance programmes for avian influenza in poultry and wild birds
1. Identification of the programme
Member state : KYPROS
Disease avian influenza in poultry and wild birds
This program is multi annual : no
Request of Union co-financing from beginning of :
2016
Page 3 of 32
ANNEX 4 : Standard requirements for the submission of surveillance programmes for avian influenza in poultry and wild birds
1.1 Contact
Name : Georgios Krasias
Phone : +35722805180
Your job type within the CA : Veterinary Officer Animal Health and Welfare Division (AHWD) Vete
Email : [email protected]
2. Description and implementation of the surveillance programme in poultry
2.1.1 Designation of the central authority in charge of supervising and coordinating the departments responsible for implementing the programme
(max. 32000 chars) :
The Veterinary Services of the Ministry of Agriculture, Rural Development and Environment are responsible for the implementation of the control programme of Avian Influenza . Within the Veterinary Services, the Animal Health and Welfare Division is responsible for the coordination and supervision of the programme. The five District Veterinary Offices with the five Rural Veterinary Stations are responsible for the collection and transport of samples to the Laboratories of the Veterinary Services.
2.1.2 System in place for the registration of holdings
Page 4 of 32
ANNEX 4 : Standard requirements for the submission of surveillance programmes for avian influenza in poultry and wild birds
(max. 32000 chars) :
BREEDING HOLDINGS The central authority has registered officially all holdings with breeding flocks according to Κ.Δ.Π. 317/2002, which transfers Directive 90/539/EΕC into Cyprus national law. LAYING FARMS The central authority has registered officially all holdings with layer flocks according to Κ.Δ.Π. 761/2003, which transfers Directives 1999/74/EC and 2002/4/EC into Cyprus national law. According to this all laying hen holdings of more than 350 laying hens should be registered with the Veterinary Services. Packing centres are registered according to Cyprus national law N. 276(I)2004. BROILER FARMS There is no legal basis for the registration of broiler farms. However the Veterinary Services keep a database with the broiler farms. TURKEYS There are no turkey breeding flocks in Cyprus. Day-old chicks are imported from Member States to broiler farms for fattening and slaughter for the period of Christmas.
2.1.3 Design (risk based surveillance or surveillance based on representative sampling taking into account criteria in Annex I of Commission Decision 2010/367/EC)
(max. 32000 chars) :
The programme will be implemented from 1st January 2016 until 31 December 2016. All samples are to be tested in the NRL of Cyprus and in case of positive results, samples will be sent to the CRL for confirmation. Surveillance is based on representative sampling. Based on the different types of poultry holdings operating in Cyprus, samples are to be taken from chickens (breeding hens, laying hens, free range laying hens and free range broilers), fattening turkeys, quails and partridges according to Commission Decision 2010/367/EU. Moreover, samples will be collected from backyard poultry in areas defined as high risk. SAMPLING Blood sampling for serological examination will be collected from 10 birds per holding or in cases of holdings consisting of more than one shed from 5 birds per shed. All holdings of each species will be included in the sampling frame for serological examination. Sampling scheme in breeding flocks All parent stock is imported to Cyprus as day-old chicks. There are 4 breeding companies, who own in total 9 approved holdings housing breeding flocks, with a total of 26 sheds. Day-old chicks are sold to
Page 5 of 32
ANNEX 4 : Standard requirements for the submission of surveillance programmes for avian influenza in poultry and wild birds
laying hen or broiler farms within Cyprus. It is estimated that 135 blood samples will be collected and tested from breeding flocks (25sheds * 5 birds sampled and 1shed * 10 birds sampled) Sampling scheme in broilers There are 3 free range broiler farms, which will be included in the sampling frame for the surveillance of Avian Influenza, with a total of 7 sheds. It is estimated that 45 blood samples will be collected and tested from free range broiler flocks (1st farm: 5 sheds*5 birds sampled per shed and each 2nd farm and 3rd farm:1 shed*10 birds sampled). Sampling scheme in layers There are 27 conventional laying hen holdings with 59 sheds. 10 blood samples will be collected from holdings having 1 shed and 5 blood samples per shed in holding with more than 1 shed. It is estimated that 340 blood samples will be collected and tested from conventional laying flocks. There are 12 free range and organic laying holdings, with a total of 29 sheds. It is estimated that 150 blood samples will be collected and tested from free range and organic laying flocks. Sampling scheme in turkey holdings There are no breeding farms for turkeys in Cyprus. Based on the number of flocks for 2014 ,it is estimated that 5 holdings will place turkeys for fattening, with a total of 8 sheds. It is estimated that 65 blood samples will be collected and tested from turkeys flocks (5 holdings with 1 shed: 5 sheds*10 birds sampled=50 samples and 1 holding with 3 sheds: 3 sheds*5 birds sampled per shed=15 samples). Sampling scheme in partridges There are 3 holdings for partridges with an average of 2 sheds per holding. It is estimated that 30 blood samples will be collected and tested from partridge flocks (6 sheds*5 birds sampled per shed). Sampling scheme for quails: There are 3 quail holdings. It is estimated that 30 blood samples will be collected and tested from quail flocks (3 sheds*10 birds sampled per shed). Sampling scheme in duck, goose and mallard holdings There are no duck, goose or mallard holdings in Cyprus. In case such holdings are operating in 2016, 20 blood samples will be collected and tested per holding. Sampling scheme in backyard poultry Blood samples from backyard poultry will be collected from high risk areas in Cyprus. According to the 2006 census there were 1247 backyard holdings in these high risk areas. It is estimated that 600 blood samples will be collected and tested from 60 backyard holdings (60 sheds * 10 birds sampled per shed).
2.1.3.1 Short description of predominant poultry population and types of poultry production
Page 6 of 32
ANNEX 4 : Standard requirements for the submission of surveillance programmes for avian influenza in poultry and wild birds
(max. 32000 chars) :
BREEDING FLOCKS GALLUS GALLUS In Cyprus there are only 4 breeding companies which have in total 9 breeding holdings and 3 hatcheries. There are in total 25 sheds housing parent stock for broilers and 1 shed housing parent stock for layers. LAYING FLOCKS GALLUS GALLUS There are 27 conventional laying hen holdings with 59 sheds and12 free range and organic laying holdings with 29 sheds. BROILER FLOCKS GALLUS GALLUS There are 97 broiler farms operating in Cyprus, out of which only 3 are free range broiler farms.
2.1.3.2 Criteria and risk factors for risk based surveillance(1)
(max. 32000 chars) :
The surveillance programme is based on Representative Sampling, according to Annex I to Commission Decision 2010/367/EU. Due to the small number of holdings in each poultry category, all poultry holdings will be tested in the framework of the surveillance programme.
(1) Including maps showing target sampling sites identified as being particularly at risk for the introduction of avian influenza virus, taking into account criteria set out in point 4 of Annex I to Commission Decision 2010/367/EC.
2.2 Target populations (2)
(max. 32000 chars) :
Samples are to be taken from chickens (breeding hens, laying hens, free range laying hens and free range broilers), fattening turkeys, quails, partridges and backyard poultry.
(2) including MS specific exceptional circumstances as described in Annex I point 3 of Commission Decision 2010/367/EU)
Page
7 o
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AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
2.2.
1 P
OU
LTRY
HO
LDIN
GS (a
) (exc
ept d
ucks
, gee
se a
nd fa
rmed
gam
e bi
rds (
wat
erfo
wl e
.g. m
alla
rds)
to b
e sa
mpl
ed
Se
rolo
gica
l inv
estig
atio
n ac
cord
ing
to A
nnex
I to
Com
mis
sion
Dec
isio
n 20
10/3
67/E
U
2016
Ta
rget
s for
yea
r
Cate
gory
: ch
icke
n br
eede
rsde
lete
thi
s ca
tego
ry
NU
TS (2
) (b)
Tota
l num
ber o
f hol
ding
s(c)
Tota
l num
ber o
f hol
ding
s to
be
sam
pled
Num
ber o
f sam
ples
per
ho
ldin
gTo
tal n
umbe
r of t
ests
Met
hod
of la
bora
tory
ana
lysis
CY0
05
510
50H
I-tes
t (H
5)x
CY0
01
130
30H
I-tes
t (H
5)x
CY0
02
220
40H
I-tes
t (H
5)x
CY0
01
115
15H
I-tes
t (H
5)x
CY0
05
510
50H
I-tes
t (H
7)x
CY0
01
130
30H
I-tes
t (H
7)x
CY0
02
220
40H
I-tes
t (H
7)x
CY0
01
115
15H
I-tes
t (H
7)x
Page
8 o
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AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
Tota
l27
0
Add
a n
ew ro
w(a
) H
oldi
ngs o
r her
ds o
r flo
cks o
r est
ablis
hmen
ts a
s app
ropr
iate
. (b
) Re
fers
to th
e lo
catio
n of
the
hold
ing
of o
rigin
. In
case
NU
TS (N
omen
clat
ure
of T
errit
oria
l Uni
ts fo
r Sta
tistic
s) c
an n
ot b
e us
ed, r
egio
n as
def
ined
in th
e pr
ogra
mm
e by
the
Mem
ber S
tate
s is r
eque
sted
(c
) To
tal n
umbe
r of h
oldi
ngs o
f one
cat
egor
y of
pou
ltry
in c
once
rned
NU
TS 2
regi
on.
Cate
gory
: la
ying
hen
sde
lete
thi
s ca
tego
ry
NU
TS (2
) (b)
Tota
l num
ber o
f hol
ding
s(c)
Tota
l num
ber o
f hol
ding
s to
be
sam
pled
Num
ber o
f sam
ples
per
ho
ldin
gTo
tal n
umbe
r of t
ests
Met
hod
of la
bora
tory
ana
lysis
CY0
020
2010
200
HI-t
est (
H5)
xC
Y00
33
1545
HI-t
est (
H5)
xC
Y00
22
2040
HI-t
est (
H5)
xC
Y00
11
2525
HI-t
est (
H5)
xC
Y00
11
3030
HI-t
est (
H5)
xC
Y00
2020
1020
0H
I-tes
t (H
7)x
CY0
03
315
45H
I-tes
t (H
7)x
CY0
02
220
40H
I-tes
t (H
7)x
CY0
01
125
25H
I-tes
t (H
7)x
CY0
01
130
30H
I-tes
t (H
7)x
Tota
l68
0
Page
9 o
f 32
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
Add
a n
ew ro
w(a
) H
oldi
ngs o
r her
ds o
r flo
cks o
r est
ablis
hmen
ts a
s app
ropr
iate
. (b
) Re
fers
to th
e lo
catio
n of
the
hold
ing
of o
rigin
. In
case
NU
TS (N
omen
clat
ure
of T
errit
oria
l Uni
ts fo
r Sta
tistic
s) c
an n
ot b
e us
ed, r
egio
n as
def
ined
in th
e pr
ogra
mm
e by
the
Mem
ber S
tate
s is r
eque
sted
(c
) To
tal n
umbe
r of h
oldi
ngs o
f one
cat
egor
y of
pou
ltry
in c
once
rned
NU
TS 2
regi
on.
Cate
gory
: fre
e ra
nge
layi
ng h
ens
dele
te t
his
cate
gory
NU
TS (2
) (b)
Tota
l num
ber o
f hol
ding
s(c)
Tota
l num
ber o
f hol
ding
s to
be
sam
pled
Num
ber o
f sam
ples
per
ho
ldin
gTo
tal n
umbe
r of t
ests
Met
hod
of la
bora
tory
ana
lysis
CY0
08
810
80H
I-tes
t (H
5)x
CY0
03
315
45H
I-tes
t (H
5)x
CY0
01
125
25H
I-tes
t (H
5)x
CY0
08
810
80H
I-tes
t (H
7)x
CY0
03
315
45H
I-tes
t (H
7)x
CY0
01
125
25H
I-tes
t (H
7)x
Tota
l30
0
Add
a n
ew ro
w(a
) H
oldi
ngs o
r her
ds o
r flo
cks o
r est
ablis
hmen
ts a
s app
ropr
iate
. (b
) Re
fers
to th
e lo
catio
n of
the
hold
ing
of o
rigin
. In
case
NU
TS (N
omen
clat
ure
of T
errit
oria
l Uni
ts fo
r Sta
tistic
s) c
an n
ot b
e us
ed, r
egio
n as
def
ined
in th
e pr
ogra
mm
e by
the
Mem
ber S
tate
s is r
eque
sted
(c
) To
tal n
umbe
r of h
oldi
ngs o
f one
cat
egor
y of
pou
ltry
in c
once
rned
NU
TS 2
regi
on.
Page
10
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
Cate
gory
: br
oile
rs (o
nly
whe
n at
risk
)de
lete
thi
s ca
tego
ry
NU
TS (2
) (b)
Tota
l num
ber o
f hol
ding
s(c)
Tota
l num
ber o
f hol
ding
s to
be
sam
pled
Num
ber o
f sam
ples
per
ho
ldin
gTo
tal n
umbe
r of t
ests
Met
hod
of la
bora
tory
ana
lysis
CY0
01
125
25H
I-tes
t (H
5)x
CY0
02
210
20H
I-tes
t (H
5)x
CY0
01
125
25H
I-tes
t (H
7)x
CY0
02
210
20H
I-tes
t (H
7)x
Tota
l90
Add
a n
ew ro
w(a
) H
oldi
ngs o
r her
ds o
r flo
cks o
r est
ablis
hmen
ts a
s app
ropr
iate
. (b
) Re
fers
to th
e lo
catio
n of
the
hold
ing
of o
rigin
. In
case
NU
TS (N
omen
clat
ure
of T
errit
oria
l Uni
ts fo
r Sta
tistic
s) c
an n
ot b
e us
ed, r
egio
n as
def
ined
in th
e pr
ogra
mm
e by
the
Mem
ber S
tate
s is r
eque
sted
(c
) To
tal n
umbe
r of h
oldi
ngs o
f one
cat
egor
y of
pou
ltry
in c
once
rned
NU
TS 2
regi
on.
Cate
gory
: fa
tteni
ng tu
rkey
sde
lete
thi
s ca
tego
ry
NU
TS (2
) (b)
Tota
l num
ber o
f hol
ding
s(c)
Tota
l num
ber o
f hol
ding
s to
be
sam
pled
Num
ber o
f sam
ples
per
ho
ldin
gTo
tal n
umbe
r of t
ests
Met
hod
of la
bora
tory
ana
lysis
CY0
05
510
50H
I-tes
t (H
5)x
CY0
01
115
15H
I-tes
t (H
5)x
CY0
05
510
50H
I-tes
t (H
7)x
Page
11
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
CY0
01
115
15H
I-tes
t (H
7)x
Tota
l13
0
Add
a n
ew ro
w(a
) H
oldi
ngs o
r her
ds o
r flo
cks o
r est
ablis
hmen
ts a
s app
ropr
iate
. (b
) Re
fers
to th
e lo
catio
n of
the
hold
ing
of o
rigin
. In
case
NU
TS (N
omen
clat
ure
of T
errit
oria
l Uni
ts fo
r Sta
tistic
s) c
an n
ot b
e us
ed, r
egio
n as
def
ined
in th
e pr
ogra
mm
e by
the
Mem
ber S
tate
s is r
eque
sted
(c
) To
tal n
umbe
r of h
oldi
ngs o
f one
cat
egor
y of
pou
ltry
in c
once
rned
NU
TS 2
regi
on.
Cate
gory
: fa
rmed
gam
e bi
rds
(gal
linac
eous
)de
lete
thi
s ca
tego
ry
NU
TS (2
) (b)
Tota
l num
ber o
f hol
ding
s(c)
Tota
l num
ber o
f hol
ding
s to
be
sam
pled
Num
ber o
f sam
ples
per
ho
ldin
gTo
tal n
umbe
r of t
ests
Met
hod
of la
bora
tory
ana
lysis
CY0
06
610
60H
I-tes
t (H
5)x
CY0
06
610
60H
I-tes
t (H
7)x
Tota
l12
0
Add
a n
ew ro
w(a
) H
oldi
ngs o
r her
ds o
r flo
cks o
r est
ablis
hmen
ts a
s app
ropr
iate
. (b
) Re
fers
to th
e lo
catio
n of
the
hold
ing
of o
rigin
. In
case
NU
TS (N
omen
clat
ure
of T
errit
oria
l Uni
ts fo
r Sta
tistic
s) c
an n
ot b
e us
ed, r
egio
n as
def
ined
in th
e pr
ogra
mm
e by
the
Mem
ber S
tate
s is r
eque
sted
(c
) To
tal n
umbe
r of h
oldi
ngs o
f one
cat
egor
y of
pou
ltry
in c
once
rned
NU
TS 2
regi
on.
Cate
gory
: ba
ckya
rd fl
ocks
dele
te t
his
cate
gory
Page
12
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
NU
TS (2
) (b)
Tota
l num
ber o
f hol
ding
s(c)
Tota
l num
ber o
f hol
ding
s to
be
sam
pled
Num
ber o
f sam
ples
per
ho
ldin
gTo
tal n
umbe
r of t
ests
Met
hod
of la
bora
tory
ana
lysis
CY0
01
247
6010
600
HI-t
est (
H5)
xC
Y00
1 24
760
1060
0H
I-tes
t (H
7)x
Tota
l1
200
Add
a n
ew ro
w(a
) H
oldi
ngs o
r her
ds o
r flo
cks o
r est
ablis
hmen
ts a
s app
ropr
iate
. (b
) Re
fers
to th
e lo
catio
n of
the
hold
ing
of o
rigin
. In
case
NU
TS (N
omen
clat
ure
of T
errit
oria
l Uni
ts fo
r Sta
tistic
s) c
an n
ot b
e us
ed, r
egio
n as
def
ined
in th
e pr
ogra
mm
e by
the
Mem
ber S
tate
s is r
eque
sted
(c
) To
tal n
umbe
r of h
oldi
ngs o
f one
cat
egor
y of
pou
ltry
in c
once
rned
NU
TS 2
regi
on.
Add
a c
ateg
ory
Tota
lsTo
tal n
umbe
r of t
ests
Tota
l pou
ltry
2016
2 79
0
2.2.
2
DU
CKS
,GEE
SE A
ND
FAR
MED
GAM
E BI
RDS
(WAT
ERFO
WL
e.g.
MAL
LARD
) HO
LDIN
GS
(a) t
o be
sam
pled
.
Sero
logi
cal i
nves
tigat
ion
acco
rdin
g to
Ann
ex I t
o Co
mm
issi
on D
ecis
ion
2010
/367
/EU
Page
13
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
2016
Ta
rget
s for
yea
r
Cate
gory
: N
/Ade
lete
thi
s ca
tego
ry
NU
TS (2
) (b)
Tota
l num
ber o
f duc
k an
d
gees
e ho
ldin
gs
Tota
l num
ber o
f duc
k an
d ge
ese
hold
ings
to b
e sa
mpl
ed
Num
ber o
f sam
ples
per
ho
ldin
gTo
tal n
umbe
r of t
ests
Met
hod
of la
bora
tory
ana
lysis
CY0
00
00
0Vi
rus
isola
tion
test
xTo
tal
0
Add
a n
ew ro
w(a
) H
oldi
ngs o
r her
ds o
r flo
cks o
r est
ablis
hmen
ts a
s app
ropr
iate
. (b
) Re
fers
to th
e lo
catio
n of
the
hold
ing
of o
rigin
. In
case
NU
TS (2
) cod
e ca
n no
t be
used
, reg
ion
as d
efin
ed in
the
prog
ram
me
by th
e M
embe
r Sta
te is
requ
este
d
Add
a c
ateg
ory
NU
TS (2
) (b)
Tota
l num
ber o
f tes
ts
Tota
l duc
ks a
nd g
eese
and
fa
rmed
gam
e bi
rds
2016
0
TOTA
LS fo
r Pou
ltry
(2.2
.1) +
Duc
ks a
nd G
eese
(2.2
.2)
and
farm
ed g
ame
bird
s for
yea
r :20
16
Page
14
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
Poul
try +
D
ucks
/Gee
se /f
arm
ed g
ame
bird
s To
tal n
umbe
r of
test
sG
rand
Tot
al
2 79
0
Gra
nd T
otal
ELI
SA
0
Gra
nd T
otal
aga
r
0
Gra
nd T
otal
HI t
ests
(H5)
1
395
Gra
nd T
otal
HI t
ests
(H7)
1
395
Gra
nd T
otal
Viru
s Is
olat
ion
test
0
Gra
nd T
otal
PCR
test
0
Gra
nd T
otal
Oth
er te
st
0
Gra
nd T
otal
Sam
plin
gs
0
2.3
Sa
mpl
ing
proc
edur
es, s
ampl
ing
perio
ds a
nd fr
eque
ncy
of te
stin
g ta
king
into
acc
ount
crit
eria
set o
ut in
Ann
ex I o
f Co
mm
issi
on D
ecis
ion
2010
/367
/EC
(max
. 320
00 c
hars
) :
Sam
plin
g fr
om a
ll ca
tego
ries
of p
oultr
y ho
ldin
gs (b
reed
ing,
con
vent
iona
l lay
ing
hen,
free
rang
e la
ying
hen
, fre
e ra
nge
broi
ler,
fatt
enin
g tu
rkey
, par
trid
ge,
quai
l and
bac
kyar
d) w
ill b
e di
strib
uted
thro
ugho
ut th
e ye
ar.
Page
15
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
2.4.
La
bora
tory
test
ing
: des
crip
tion
of th
e la
bora
tory
test
s use
d an
d fo
llow
up
inve
stig
atio
ns ta
king
into
acc
ount
crit
eria
se
t out
in th
e D
iagn
ostic
Man
ual f
or a
vian
influ
enza
(Com
mon
Dec
isio
n 20
06/4
37/E
C)
Des
crip
tion
of th
e us
ed se
rolo
gica
l tes
ts :
(max
320
00 c
hars
)
Acc
ordi
ng to
Cha
pter
VIII
of t
he A
nnex
to C
omm
issi
on D
ecis
ion
2006
/437
/EC.
H
I ser
olog
ical
test
ing
is u
sed
for t
he p
rese
nt o
f ant
ibod
ies
H5
and
H7
subt
ypes
Th
e re
agen
ts re
quire
d fo
r the
se te
sts
are
isot
onic
PBS
(0.0
1 M
), pH
7.0
–7.2
, red
blo
od c
ells
(RBC
s) ta
ken
from
a m
inim
um o
f thr
ee S
PF c
hick
ens
and
pool
ed
into
an
equa
l vol
ume
of A
lsev
er´s
sol
utio
n an
d H
5/ H
7 an
tigen
s. C
ells
are
was
hed
thre
e tim
es in
PBS
bef
ore
use
as a
1%
(pac
ked
cell
v/v)
sus
pens
ion.
Po
sitiv
e an
d ne
gativ
e co
ntro
l ant
igen
s an
d an
tiser
a ar
e ru
n w
ith e
ach
test
. Pr
oced
ure:
i)
D
ispe
nse
0.02
5 m
l of P
BS in
to e
ach
wel
l of a
pla
stic
V-b
otto
med
mic
rotit
re p
late
. ii)
Pl
ace
0.02
5 m
l of s
erum
into
the
first
wel
l of t
he p
late
. iii
)
Mak
e tw
ofol
d di
lutio
ns o
f 0.0
25 m
l vol
umes
of t
he s
erum
acr
oss
the
plat
e.
iv)
A
dd 4
HA
U o
f ant
igen
H5/
H7
in 0
.025
ml t
o ea
ch w
ell a
nd le
ave
for a
min
imum
of 3
0 m
inut
es a
t roo
m te
mpe
ratu
re (i
.e. a
bout
20°
C) o
r 60
min
utes
at
4°C.
v)
A
dd 0
.025
ml o
f 1%
(v/v
) chi
cken
RBC
s to
eac
h w
ell a
nd m
ix g
ently
, allo
w th
e RB
Cs to
set
tle fo
r abo
ut 4
0 m
inut
es a
t roo
m te
mpe
ratu
re, i
.e. a
bout
20°
C, o
r for
60
min
utes
at 4
°C if
am
bien
t tem
pera
ture
s ar
e hi
gh, b
y w
hich
tim
e co
ntro
l RBC
s sh
ould
hav
e fo
rmed
a d
istin
ct b
utto
n.
vi)
T
he H
I titr
e is
the
high
est d
ilutio
n of
ser
um c
ausi
ng c
ompl
ete
inhi
bitio
n of
4 H
AU
of a
ntig
en. T
he a
gglu
tinat
ion
is a
sses
sed
by ti
lting
the
plat
es. O
nly
thos
e w
ells
in w
hich
the
RBCs
str
eam
at t
he s
ame
rate
as
the
cont
rol w
ells
(con
tain
ing
0.02
5 m
l RBC
s an
d 0.
05 m
l PBS
onl
y) a
re c
onsi
dere
d to
sho
w
inhi
bitio
n.
vii)
T
he v
alid
ity o
f res
ults
are
ass
esse
d ag
ains
t a n
egat
ive
cont
rol s
erum
, whi
ch s
houl
d no
t giv
e a
titre
>1/
4 (>
22 o
r >lo
g2 2
whe
n ex
pres
sed
as th
e re
cipr
ocal
), an
d a
posi
tive
cont
rol s
erum
for w
hich
the
titre
sho
uld
be w
ithin
one
dilu
tion
of th
e kn
own
titre
.
Page
16
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
3.
Des
crip
tion
and
impl
emen
tatio
n of
the
surv
eilla
nce
prog
ram
me
in w
ild b
irds
3.1.
1 D
esig
natio
n of
the
cent
ral a
utho
rity
in c
harg
e of
supe
rvis
ing
and
coor
dina
ting
the
depa
rtm
ents
resp
onsi
ble
for i
mpl
emen
ting
the
prog
ram
me
and
rele
vant
col
labo
ratin
g pa
rtne
rs (e
.g. e
pide
mio
logi
sts,
orni
thol
ogis
ts, n
atur
e bi
rd o
bser
vatio
n an
d hu
nter
or
gani
satio
ns).
(max
. 320
00 c
hars
) :
The
Vete
rinar
y Se
rvic
es o
f the
Min
istr
y of
Agr
icul
ture
, Rur
al D
evel
opm
ent a
nd E
nviro
nmen
t are
resp
onsi
ble
for t
he im
plem
enta
tion
of th
e co
ntro
l pr
ogra
mm
e of
Avi
an In
fluen
za .
With
in th
e Ve
terin
ary
Serv
ices
, the
Ani
mal
Hea
lth a
nd W
elfa
re D
ivis
ion
is re
spon
sibl
e fo
r the
coo
rdin
atio
n an
d su
perv
isio
n of
the
prog
ram
me.
The
five
D
istr
ict V
eter
inar
y O
ffice
s w
ith th
e fiv
e Ru
ral V
eter
inar
y St
atio
ns a
re re
spon
sibl
e fo
r the
col
lect
ion
and
tran
spor
t of s
ampl
es to
the
Labo
rato
ries
of th
e Ve
terin
ary
Serv
ices
.
3.1.
2 D
escr
iptio
n an
d de
limita
tion
of th
e ge
ogra
phic
al a
nd a
dmin
istr
ativ
e ar
eas i
n w
hich
the
prog
ram
me
is to
be
appl
ied
max
. 320
00 c
hars
) :
Sam
ples
for P
CR w
ill b
e co
llect
ed fr
om w
ild b
irds
in h
igh
risk
area
s.
Page
17
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
Colle
ctio
n of
dea
d or
mor
ibun
d w
ild b
irds
for s
ampl
ing
in th
e fr
ame
of th
e A
.I. s
urve
illan
ce p
rogr
amm
e is
per
form
ed in
col
labo
ratio
n w
ith th
e G
ame
fund
, a
gove
rnm
enta
l ser
vice
. It m
ust b
e no
ted
that
the
pres
ence
in C
ypru
s of
bird
s lis
ted
in p
art 2
of A
nnex
II o
f Com
mis
sion
Dec
isio
n 20
10/3
67/E
C de
pend
s m
ainl
y on
the
wea
ther
con
ditio
ns in
the
isla
nd d
urin
g th
e w
inte
r and
that
acc
ordi
ng to
the
Gam
e Fu
nd’s
dat
a th
e Be
an G
oose
(Ans
er fa
balis
) doe
s no
t pas
s fr
om C
ypru
s.
Pass
ive
surv
eilla
nce
Bird
s fo
und
dead
or m
orib
und
in h
igh
risk
area
s w
ill b
e co
llect
ed a
nd e
xam
ined
for A
I viru
s.
3.1.
3 Es
timat
ion
of th
e lo
cal a
nd/o
r mig
rato
ry w
ildlif
e po
pula
tion
(max
. 320
00 c
hars
) :
Mon
itorin
g of
loca
l and
mig
rato
ry w
ildlif
e po
pula
tion
is p
erfo
rmed
by
the
Gam
e Fu
nd. A
ccor
ding
to it
s re
port
s th
ere
was
an
incr
ease
in th
e nu
mbe
r of
mig
rato
ry b
irds,
incl
udin
g w
ater
fow
l, un
til th
e 1s
t sem
este
r of 2
013,
due
to th
e hi
gher
leve
r of r
ainf
all.
How
ever
, dur
ing
the
2nd
sem
este
r of 2
013
and
the
begi
nnin
g of
201
4, d
roug
ht c
ondi
tions
wer
e ob
serv
ed in
the
regi
on. I
n ge
nera
l the
num
ber o
f mig
rato
ry w
ild b
irds
depe
nds
on th
e w
eath
er c
ondi
tions
(r
ainf
all a
nd te
mpe
ratu
re) e
ach
year
.
3.2
Des
ign,
crit
eria
, ris
k fa
ctor
s and
targ
et p
opul
atio
n(3)
(max
. 320
00 c
hars
) :
The
surv
eilla
nce
prog
ram
me
for A
vian
Influ
enza
in w
ild b
irds
aim
s at
the
early
det
ectio
n of
HPA
I viru
ses
of th
e su
btyp
e H
5N1
in w
ild b
irds
in o
rder
to
prev
ent t
he in
trod
uctio
n of
the
infe
ctio
n in
pou
ltry.
Sa
mpl
es fo
r PCR
will
be
colle
cted
from
wild
bird
s in
hig
h ris
k ar
eas.
Page
18
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
Gam
e fu
nd is
a g
over
nmen
tal s
ervi
ce th
at c
oope
rate
s w
ith v
eter
inar
y se
rvic
es a
nd p
rovi
des
dead
or m
orib
und
wild
bird
s th
at c
an b
e sa
mpl
ed fo
r the
A.I.
su
rvei
llanc
e pr
ogra
m. T
he s
ampl
es w
ill b
e ei
ther
a) o
roph
aryn
geal
and
/or c
loac
al s
wab
s fr
om m
orib
und,
or b
) fae
ces,
clo
acal
sw
abs,
trac
heal
/or
opha
ryng
eal s
wab
s or
tiss
ues
(bra
in, h
eart
, lun
g, k
idne
y an
d in
test
ines
) fro
m d
ead
bird
s.
It m
ust b
e no
ted
that
the
pres
ence
in C
ypru
s of
bird
s lis
ted
in p
art 2
of A
nnex
II o
f Com
mis
sion
Dec
isio
n 20
10/3
67/E
U d
epen
ds m
ainl
y on
the
wea
ther
co
nditi
ons
in th
e is
land
dur
ing
the
win
ter.
It ca
n no
t be
ensu
red
that
sam
ples
from
all
the
spec
ies
of th
ese
wild
bird
s w
ill b
e co
llect
ed. I
t mus
t als
o be
not
ed
that
acc
ordi
ng to
the
Gam
e Fu
nd’s
dat
a th
e Be
an G
oose
(Ans
er fa
balis
) doe
s no
t pas
s fr
om C
ypru
s.
Pass
ive
surv
eilla
nce
Bird
s fo
und
dead
or m
orib
und
in h
igh
risk
area
s w
ill b
e co
llect
ed a
nd e
xam
ined
for A
I viru
s. B
ased
on
the
expe
rienc
e of
pre
viou
s ye
ars,
it is
exp
ecte
d to
ex
amin
e ar
ound
180
sam
ples
from
app
roxi
mat
ely
180
bird
s.
(3)
Are
as a
t ri
sk (
wet
land
s in
par
ticu
lar
whe
re l
inks
wit
h hi
gh d
ensi
ty p
oult
ry p
opul
atio
ns),
pre
viou
s po
siti
ve f
indi
ngs
as r
efer
red
to i
n po
int
2 of
Par
t 1
of A
nnex
II t
o Co
mm
issi
on D
ecis
ion
2010
/367
/EC
shou
ld b
e ta
ken
into
acc
ount
and
if
poss
ible
com
plem
ente
d by
a m
ap.
3.2.
1 W
ILD
BIR
DS
focu
ssed
on
targ
et sp
ecie
s
Inve
stig
atio
ns a
ccor
ding
to th
e su
rvei
llanc
e pr
ogra
mm
e se
t out
in c
onfo
rmity
with
Par
t 2 o
f Ann
ex II
to D
ecis
ion
2010
/367
/EC
2016
Ta
rget
s for
yea
r
Page
19
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
NU
TS (2
) cod
e/re
gion
(a)
Tota
l num
ber o
f bird
s to
be
sam
pled
Estim
ated
tota
l num
ber o
f sa
mpl
es to
be
take
n fo
r pa
ssive
sur
veilla
nce
Type
of t
est
Num
ber o
f tes
ts
CY0
018
018
0PC
R te
st18
0x
Tota
l
Add
a n
ew ro
w
(a)
Refe
rs to
the
plac
e of
col
lect
ion
of b
irds/
sam
ples
. In
case
NU
TS 2
(Nom
encl
atur
e of
Ter
ritor
ial U
nits
for S
tatis
tics)
can
not
be
used
, reg
ion
as d
efin
ed in
the
prog
ram
me
by
the
Mem
ber S
tate
is re
ques
ted.
Ple
ase
fill-i
n th
ese
valu
es d
irect
ly in
the
field
.
Tota
l num
ber o
f tes
ts
Tota
l num
ber o
f tes
ts
180
Tota
l Viru
s is
olat
ion
test
s0
Tota
l PCR
test
s18
0
Tota
l Oth
er te
sts
0
3.3
Sam
plin
g pr
oced
ures
and
sam
plin
g pe
riods
max
320
00 c
hars
:
Sam
plin
g fr
om w
ild b
irds
foun
d de
ad o
r mor
ibun
d w
ill p
erfo
rmed
thro
ugho
ut th
e ye
ar.
All
sam
ples
will
be
subm
itted
to th
e La
bora
torie
s of
the
Vete
rinar
y Se
rvic
es.
Page
20
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
3.4
Labo
rato
ry te
stin
g : d
escr
iptio
n of
the
labo
rato
ry te
sts u
sed
taki
ng in
to a
ccou
nt c
riter
ia se
t out
in th
e D
iagn
ostic
M
anua
l for
avi
an in
fluen
za (C
omm
issi
on D
ecis
ion
2006
/437
/EC)
max
320
00 c
hars
:
Acc
ordi
ng to
Cha
pter
V a
nd C
hapt
er V
I of t
he A
nnex
to C
omm
issi
on D
ecis
ion
2006
/437
/EC.
Re
alTi
me-
RT-P
CR
1)
E
xtra
ct R
NA
usi
ng M
agN
A P
ure
Com
pact
Nuc
leic
Aci
d Is
olat
ion
Kit a
nd M
agN
A p
ure
com
pact
inst
rum
ent.
2)
D
etec
tion
of in
fluen
za A
mat
rix g
ene
by re
al ti
me
Taqm
an®
RT-P
CR
The
reag
ents
requ
ired
for t
hese
test
s ar
e:
a) P
rimer
s an
d pr
obe
Sep1
AG
A T
GA
GTC
TTC
TA
A C
CG A
GG
TCG
Se
p2 T
GC
AA
A A
AC
ATC
TTC
AA
G T
CT C
TG
SePR
O F
AM
-TCA
GG
C CC
C CT
C A
AA
GCC
GA
-TA
MRA
b)
Rea
l tim
e PC
R m
aste
r mix
whi
ch u
tilis
es th
e Q
iage
n O
nest
ep R
T-PC
R ki
t (Ca
t No.
210
212)
. Vo
lum
es fo
r the
abo
ve m
aste
r mix
are
indi
cate
d be
low
: D
EPC
trea
ted
wat
er 1
3.75
μl
(x5)
Qia
gen
1 st
ep R
T PC
R bu
ffer 5
μl
Rox
ref d
ye(p
re d
ilute
d 1:
500)
in D
EPC
wat
er 0
.375
μl
Qia
gen
dNTP
mix
1μl
Se
p1(5
0μM
) 0,2
μl
Sep2
(50μ
M) 0
,2μl
Se
PRO
(30μ
M) 0
.25μ
l 25
mM
mag
nesi
um c
hlor
ide
1.25
μl
RNA
sin
(40U
/μl)
0.1μ
l Q
iage
n 1
step
RT P
CR e
nzym
e m
ix 1
μl
Aliq
uot 2
3μl o
f mas
ter m
ix p
er w
ell o
f the
real
tim
e pl
ate/
strip
(s) a
nd a
dd 2
μl o
f the
sam
ple
RNA
. Onc
e RN
A is
add
ed, p
late
/str
ip(s
) are
load
ed o
nto
a
Page
21
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
Ligh
tcyc
ler 9
6 re
al ti
me
Mac
hine
. Th
e re
actio
ns a
re in
cuba
ted
with
the
follo
win
g th
erm
ocyc
ling
prof
ile:
RT s
tep:
50°
C fo
r 30
min
s,
95°C
for 1
5 m
ins
PCR
step
(x 4
0 cy
cles
): 95
°C fo
r 10
secs
60
°C fo
r 20
secs
Th
e co
llect
ion
of fl
uore
scen
ce d
ata
is d
one
durin
g th
e 60
°C a
nnea
ling
step
usi
ng th
e RO
X an
d FA
M fi
lters
. Cr
iteria
for a
sses
sing
whe
ther
a s
ampl
e is
pos
itive
or n
egat
ive
are
as fo
llow
s:
Ct v
alue
<30
- sa
mpl
es a
re M
gen
e po
sitiv
e Ct
val
ue 3
0-35
with
sig
moi
dal/l
ogar
ithm
ic a
ppea
ranc
e –
sam
ples
are
w
eak
posi
tive.
Ct
val
ue >
35 s
ampl
es a
re n
egat
ive
if th
e am
plifi
catio
n pl
ot h
as a
line
ar s
hape
. The
se p
lots
may
repr
esen
t spu
rious
pro
be d
egra
datio
n or
non
spec
ific
fluor
esce
nce.
4.
Des
crip
tion
of th
e ep
idem
iolo
gica
l situ
atio
n of
the
dise
ase
in p
oultr
y du
ring
the
last
five
yea
rsm
ax 3
2000
cha
rs :
No
avia
n in
fluen
za v
irus
was
det
ecte
d in
sam
ples
col
lect
ed fr
om p
oultr
y fa
rms
in th
e fr
amew
ork
of th
e su
rvei
llanc
e pr
ogra
mm
es c
arrie
d ou
t by
the
Vete
rinar
y Se
rvic
es d
urin
g th
e la
st y
ears
.
Page
22
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
5.
Des
crip
tion
of th
e ep
idem
iolo
gica
l situ
atio
n of
the
dise
ase
in w
ild b
irds d
urin
g th
e la
st fi
ve y
ears
(max
. 320
00 c
hars
) :
No
HPA
I viru
s w
as d
etec
ted
in w
ild b
irds
sam
pled
in th
e fr
amew
ork
of th
e su
rvei
llanc
e pr
ogra
mm
es. H
owev
er H
1N1
was
det
ecte
d in
wild
bird
s in
Aug
ust
2006
.
6.
Mea
sure
s in
plac
e as
rega
rds t
he n
otifi
catio
n of
the
dise
ase
(max
. 320
00 c
hars
) :
Acc
ordi
ng to
Ani
mal
Hea
lth L
aws
of 2
001
until
201
4, a
ny c
ase
or s
uspi
cion
of A
vian
influ
enza
sho
uld
be n
otifi
ed to
the
Vete
rinar
y Se
rvic
es.
Conf
irmed
cas
es w
ill b
e no
tifie
d to
the
EU th
roug
h A
DN
S an
d O
IE a
ccor
ding
to th
e re
leva
nt le
gisl
atio
n pr
ovis
ions
.
7.
Cost
s
7.1
Det
aile
d an
alys
is o
f the
cos
ts
Page
23
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
7.1.
1 P
oultr
y in
clud
ing
duck
s, ge
ese
and
farm
ed g
ame
bird
s
(max
. 320
00 c
hars
) :
The
cost
of t
he s
urve
illan
ce p
rogr
amm
e fo
r pou
ltry
cons
ists
of t
he c
ost f
or th
e sa
mpl
ing
of th
e bi
rds
and
the
cost
of t
he te
sts
perf
orm
ed o
n sa
mpl
es
colle
cted
.
7.1.
2
Wild
bird
s
(max
. 320
00 c
hars
) :
The
cost
of t
he s
urve
illan
ce p
rogr
amm
e fo
r pou
ltry
cons
ists
of t
he c
ost f
or th
e sa
mpl
ing
of th
e bi
rds
and
the
cost
of t
he te
sts
perf
orm
ed o
n sa
mpl
es
colle
cted
.
Page
24
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
7.2
Sum
mar
y of
the
annu
al c
osts
:
7.2.
1
Poul
try
surv
eilla
nce
incl
udin
g du
cks,
gees
e an
d fa
rmed
gam
e bi
rds
Det
aile
d an
alys
is o
f the
cos
t of t
he p
rogr
amm
e - p
oultr
y
2016
Ta
rget
s for
yea
r
Labo
rato
ry te
stin
g Met
hods
of l
abor
ator
y an
alys
isN
umbe
r of t
ests
Indi
cativ
e un
itary
test
cos
t (pe
r m
etho
d) in
€ (*
)To
tal c
ost (
€)
ELIS
A te
st0
3.26
0
agar
gel
imm
une
diffu
sion
test
01.
80
HI-T
est f
or H
5 (s
pecif
y nu
mbe
r of t
ests
for H
5)13
959.
6413
447.
8
HI-T
est f
or H
7 (s
pecif
y nu
mbe
r of t
ests
for H
7)13
959.
6413
447.
8
Viru
s iso
latio
n te
st0
37.8
80
PCR
test
019
.74
0
Oth
er te
st0
00
Sam
plin
g
Num
ber o
f sam
ples
Uni
tary
cos
t in
€ (*
)To
tal c
ost (
€)
Page
25
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
Sam
ples
02.
810
Oth
er m
easu
res
Num
ber o
f sam
ples
Uni
tary
cos
t in
€To
tal c
ost (
€)
Sam
ples
1395
2.81
3919
,95
xA
dd a
new
row
Tota
l pou
ltry,
duc
k, g
eese
, far
med
gam
e bi
rds
Te
stin
g +
Sam
plin
g +
Oth
er m
easu
res
30 8
15,5
5 €
(*)
as p
er c
ofin
anci
ng d
ecis
ion
for 2
014
prog
ram
mes
Page
26
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
7.2.
2
Wild
bird
surv
eilla
nce
Det
ail a
naly
sis o
f the
cos
t of t
he p
rogr
amm
e - w
ild b
irds
2016
Ta
rget
s for
yea
r
Labo
rato
ry te
stin
g Met
hods
of l
abor
ator
y an
alys
isN
umbe
r of t
ests
Uni
tary
test
cos
t (pe
r met
hod)
in €
(*)
Tota
l cos
t (€)
Viru
s iso
latio
n te
st0
37.8
80
PCR
test
180
19.7
435
53.2
Oth
er c
ost
00
0
Del
iver
y of
wild
ani
mal
s
No
of w
ild b
irds
Elig
ible
cos
t in
€ (*
)To
tal c
ost (
€)
Del
ivery
of w
ild a
nim
als
00
0
Oth
er m
easu
res
Num
ber
Uni
tary
cos
t in
€To
tal c
ost (
€)
Page
27
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
-0
00
xA
dd a
new
row
Tota
l wild
bird
s T
estin
g +
Deliv
ery
+ O
ther
mea
sure
s3
553,
20 €
(*)
as p
er c
ofin
anci
ng d
ecis
ion
for 2
014
prog
ram
mes
TOTA
LS fo
r Pou
ltry,
duc
k, g
eese
, far
med
gam
e bi
rds (
7.2.
1) +
WIL
D B
IRD
S (7
.2.2
) fo
r yea
r :20
16
Tota
l Cos
t
Gra
nd T
otal
Pou
ltry,
Duc
ks/G
eese
/Far
med
gam
e bi
rds
+ W
ILD
BIRD
S 34
368,
75
Page
28
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
C. F
inan
cial
info
rmat
ion
1. I
dent
ifica
tion
of t
he im
plem
entin
g en
titie
s -
fin
anci
al c
ircu
its/f
low
s Id
entif
y an
d de
scribe
th
e en
titie
s w
hich
w
ill be
in
ch
arge
of
im
plem
entin
g th
e el
igib
le m
easu
res
plan
ned
in th
is
prog
ram
me
whi
ch c
osts
will
con
stitu
te t
he r
eim
burs
men
t/pa
ymen
t cl
aim
to
the
EU.
Des
crib
e th
e fin
anci
al f
low
s/ci
rcui
ts
follo
wed
. Ea
ch o
f th
e fo
llow
ing
para
grap
hs (
from
a t
o e)
sha
ll be
fill
ed o
ut if
EU
cof
inan
cing
is r
eque
sted
for
the
rel
ated
mea
sure
.
a) I
mpl
emen
ting
entit
ies
- s
amp
ling:
who
per
form
the
off
icia
l sam
plin
g? W
ho p
ays?
(e
.g.
auth
oris
ed p
riva
te v
ets
perf
orm
the
sam
plin
g an
d ar
e pa
id b
y th
e re
gion
al v
eter
inar
y se
rvic
es (
stat
e bu
dget
);
sam
plin
g eq
uipm
ent
is p
rovi
ded
by t
he p
riva
te l
abor
ator
y te
stin
g th
e sa
mpl
es w
hich
inc
lude
s th
e pr
ice
in t
he i
nvoi
ce
whi
ch is
pai
d by
the
loca
l sta
te v
eter
inar
y se
rvic
es (
stat
e bu
dget
))
(max
. 320
00 c
hars
) :
The
sam
plin
gs fo
r the
sur
veill
ance
pro
gram
me
for p
oultr
y is
per
form
ed b
y Ve
terin
ary
Serv
ices
’ per
sonn
el.
Conc
erni
ng th
e su
rvei
llanc
e pr
ogra
mm
e fo
r wild
bird
s ,G
ame
fund
is a
gov
ernm
enta
l ser
vice
that
coo
pera
tes
with
the
vete
rinar
y se
rvic
es a
nd p
rovi
des
dead
or m
orib
und
wild
bird
s th
at c
an b
e sa
mpl
ed fo
r the
A.I.
sur
veill
ance
pro
gram
.
b) I
mpl
emen
ting
entit
ies
- t
esti
ng:
who
per
form
s th
e te
stin
g of
the
off
icia
l sam
ples
? W
ho p
ays?
(e
.g.
regi
onal
pub
lic la
bora
tories
per
form
the
tes
ting
of o
ffic
ial s
ampl
es a
nd c
osts
rel
ated
to
this
tes
ting
are
entir
ely
paid
by
the
sta
te b
udge
t)
Page
29
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
(max
. 320
00 c
hars
) :
The
Labo
rato
ries
of th
e Ve
terin
ary
Serv
ices
will
per
form
all
the
test
ing
of o
ffici
al s
ampl
es.
c) I
mpl
emen
ting
entit
ies
- c
omp
ensa
tion
: w
ho p
erfo
rms
the
com
pens
atio
n? W
ho p
ays?
(e
.g.
com
pens
atio
n is
pai
d by
the
cen
tral
leve
l of
the
stat
e ve
terina
ry s
ervi
ces,
or
com
pens
atio
n is
pai
d by
an
insu
ranc
e fu
nd f
ed b
y co
mpu
lsor
y fa
rmer
s co
ntribu
tion)
(max
. 320
00 c
hars
) :
N/A
d) I
mpl
emen
ting
entit
ies
- v
acci
nat
ion
: w
ho p
rovi
des
the
vacc
ine
and
who
per
form
s th
e va
ccin
atio
n? W
ho p
ays
the
vacc
ine?
Who
pay
s th
e va
ccin
ator
? (e
.g.
farm
ers
buy
thei
r va
ccin
e to
the
priva
te v
ets,
sen
d th
e pa
id i
nvoi
ces
to t
he l
ocal
sta
te v
eter
inar
y se
rvic
es w
hich
re
imbu
rse
the
farm
ers
of t
he f
ull a
mou
nt a
nd t
he v
acci
nato
r is
pai
d by
the
reg
iona
l sta
te v
eter
inar
y se
rvic
es)
(max
. 320
00 c
hars
) :
Page
30
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
e) I
mpl
emen
ting
entit
ies
- o
ther
ess
enti
al m
easu
res:
who
im
plem
ent
this
mea
sure
? W
ho p
rovi
de t
he e
quip
men
t/se
rvic
e? W
ho p
ays?
(max
. 320
00 c
hars
) :
N/A
2 Co-
finan
cing
rat
e (
see
prov
isio
ns o
f ap
plic
able
Wor
k Pr
ogra
mm
e)
The
max
imum
co-
fina
ncin
g ra
te i
s in
gen
eral
fix
ed a
t 50
%. H
owev
er b
ased
on
prov
isio
ns o
f A
rtic
le 5
.2 a
nd 5
.3 o
f th
e Re
gula
tion
(EU
) N
o 65
2/20
14,
we
requ
est
that
the
co-
fina
ncin
g ra
te f
or t
he r
eim
burs
emen
t of
the
eli
gibl
e co
sts
wou
ld b
e in
crea
sed:
Up
to 7
5% fo
r the
mea
sure
s det
aile
d be
low
Up
to 1
00%
for t
he m
easu
res d
etai
led
belo
w
Page
31
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
3. S
ourc
e of
fun
ding
of
elig
ible
mea
sure
s
All
elig
ible
mea
sure
s fo
r w
hich
cof
inan
cing
is r
eque
sted
and
rei
mbu
rsm
ent
will
be
clai
med
are
fin
ance
d by
pub
lic f
unds
.
yes
no
Page
32
of 3
2
AN
NEX
4 :
Sta
ndar
d re
quirem
ents
for
the
sub
mis
sion
of
surv
eilla
nce
prog
ram
mes
for
avi
an
influ
enza
in p
oultr
y an
d w
ild b
irds
Atta
chm
ents
IMP
OR
TAN
T :
1) T
he m
ore
files
you
atta
ch, t
he lo
nger
it ta
kes
to u
ploa
d th
em .
2)
Thi
s at
tach
men
t file
s sh
ould
hav
e on
e of
the
form
at li
sted
her
e : j
pg, j
peg,
tiff,
tif,
xls,
xls
x, d
oc, d
ocx,
ppt
, ppt
x, b
mp,
pna
.
3) T
he to
tal f
ile s
ize
of th
e at
tach
ed fi
les
shou
ld n
ot e
xcee
d 2
500K
b (+
- 2.5
Mb)
. You
will
rece
ive
a m
essa
ge w
hile
atta
chin
g w
hen
you
try to
load
too
muc
h.
4) IT
CA
N T
AK
E S
EVER
AL
MIN
UTE
S TO
UPL
OA
D A
LL T
HE
ATT
AC
HE
D F
ILE
S. D
on't
inte
rrup
t the
upl
oadi
ng b
y cl
osin
g th
e pd
f and
wai
t unt
il yo
u ha
ve re
ceiv
ed a
S
ubm
issi
on N
umbe
r! 5)
Onl
y us
e le
tters
from
a-z
and
num
bers
from
1-1
0 in
the
atta
chm
ent n
ames
, oth
erw
ise
the
subm
issi
on o
f the
dat
a w
ill n
ot w
ork.
List
of a
ll at
tach
men
ts
Atta
chm
ent n
ame
File
will
be s
aved
as
(onl
y a-
z an
d 0-
9 an
d -_
) :Fi
le s
ize
6665
_417
5.oc
x66
65_4
175.
ocx
19 k
b
6665
_417
6.jp
g66
65_4
176.
jpg
377
kb
Tota
l size
of a
ttach
men
ts :
397
kb
Atta
chm
ents
in R
ED h
ave
an a
ttach
men
t ext
ensi
on th
at is
not
allo
wed
. Ple
ase
rem
ove
the
file,
oth
erw
ise
subm
issi
on w
ill n
ot w
ork.