anther culture - dr amit kr dutta
TRANSCRIPT
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Anther
CultureDr. Amit Kumar Dutta,
Ph.D
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At a precise & critical stage, it is possible to establish the anther culture that will give rise to haploid plantlets by careful selection of developing anthers.
Anther
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Haploid plants contain a single set of chromosomes.
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The use of Anther culture offers an important tool to the conventional plant breeder as a way of obtaining haploid plants for selection of characters and inclusion in the breeding programme.
Anther…..!
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W Tulecke 1953
First observed mature pollen grains can be induced to proliferate in culture to form
haploid callus.
S Guha & Maheswari 1964
Direct development of embryos from microspore by the culture of anther.
Look Back Before……!
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J P Bourgin & J P Nitsch 1967
Haploid Plantlets from Anther Culture
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Practical As well as Fundamental Point of View, Anther Culture open up a new avenue
in the field of Plant Science.
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Anther Culture
It is a technique by which the developing anthers at a precise and critical stage are excised aseptically from unopened flower bud and are cultured on a nutrient medium.
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Cultured Anther develop into callus tissue or embryoids that give rise to haploid plantlets either through organogenesis or embryogenesis.
Anther Culture
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Principle of Anther Culture
Production of Haploid Plants exploiting the totipotency of microspore and the occurrence of single set of chromosome(n) in microspore.
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Protocol
Collect the flower bud at the onset of flowering.
Transfer the selected flower buds to the LAF.
Split the side of the bud to remove the anthers.
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Anthers are placed on Agar Solidified basal MS Medium.
Kept for pollen embryogenesis formation in dark places.
Protocol
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Cultures are incubated at 24 – 280C in a 14hrs daylight.
After 1wk, transfer to larger pots.
Plants will mature and finally flower.
Protocol
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Utility of Anther Culture for Basic Research
Haploids derived from anther culture are useful in cytogenetic studies.
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By comparing the heterozygous diploid with haploid or homozygous diploid population, recessive phenotypic characters can be identified very easily.
Utility ………
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Critical genetic analysis of haploid population derived from individual microspore.
Utility ………
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Series of cell division and mode of differentiation starting from single cell and ending in whole organism can be studied under microspore..
Utility ………
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Culture of isolated pollen provides a novel experimental system for the study of factor controlling pollen embryogenesis of higher plants.
Utility ………
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Genetic analysis could be performed on haploid population to establish inheritance patterns.
Utility ………
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Thank You…..!
Any Difficulties Contact to Respective Teacher