Ž .Fitoterapia 72 2001 409�411

Short report

Antibacterial activity of luteoforol fromBridelia crenulata

N. Ramesha, M.B. Viswanathana,�, A. Saraswathy b,P. Brindhab, K. Balakrishnac,P. Lakshmanaperumalsamy d

aSri Paramakalyani Centre for En�ironmental Sciences, Manonmaniam Sundaranar Uni�ersity,Alwarkurichi 627 412, Tamil Nadu, India

bCaptain Srini�asa Murti Drug Research Institute for Ayur�eda, Chennai 600 106, Tamil Nadu,India

cCentral Research Institute for Siddha, Chennai 600 106, Tamil Nadu, IndiadDepartment of En�ironmental Sciences, Bharathiar Uni�ersity, Coimbatore 641 046,

Tamil Nadu, India

Received 11 September 2000; accepted in revised form 14 October 2000

Abstract

Ž .The methanol extract of Bridelia crenulata stem bark 50�1.5625 mg�ml and its isolatedŽ .luteoforol 2�0.25 mg�ml showed concentration-dependent inhibiting activity against all 10

tested bacteria. � 2001 Elsevier Science B.V. All rights reserved.

Keywords: Bridelia crenulata; Antibacterial activity; Flavonoids; Luteoforol

Ž . � �Plant. Bridelia crenulata Roxb. Euphorbiaceae 1,2 stem bark was collected fromAlagarkovil, Dindugal district, Tamil Nadu, India, during May 1999. A voucher

Ž .specimen M.B. Viswanathan 3127 has been deposited in the herbarium of SriParamakalyani Centre for Environmental Sciences, Alwarkurichi, Tamil Nadu,India.

� Corresponding author.

0367-326X�01�$ - see front matter � 2001 Elsevier Science B.V. All rights reserved.Ž .PII: S 0 3 6 7 - 3 2 6 X 0 0 0 0 3 1 7 - 8

()

N.R

amesh

etal.�F

itoterapia72

2001409

�411410

Table 1Antibacterial activity of methanol extract and luteoforol from Bridelia crenulata stem bark

Ž .Bacteria Diameter of zone of inhibition mmaŽ . Ž .Methanol extract mg�ml Luteoforol mg�ml Standard

50 25 12.5 6.25 3.125 1.5625 2 1 0.5 0.25

Ž .Aeromonas hydrophila 28 26 24 20 17 16 22 20 16 14 38 CeŽ .Chromobacterium �iolaceum 24 22 20 18 17 17 35 29 26 22 24 CeŽ .Escherichia coli 26 25 23 20 18 18 32 27 25 22 28 ChŽ .Klebsiella pneumoniae 33 29 28 25 24 20 30 28 24 20 32 NvŽ .Pseudomonas aeruginosa 30 28 25 23 20 20 25 24 23 18 26 CeŽ .Salmonella typhi 29 27 27 23 20 18 22 18 18 16 23 CeŽ .Vibrio cholerae 28 22 22 20 20 18 24 22 20 15 36 TrŽ .Vibrio parahaemoliticus 26 24 20 18 18 16 20 18 18 14 24 ErŽ .Bacillus subtilis 30 25 25 22 20 18 35 28 24 20 33 NvŽ .Staphylococcus aureus 27 23 20 18 17 17 28 24 22 20 31 Nv

a Ž . Ž . Ž . Ž . ŽCe, ceftriaxone 30 mcg�disc ; Ch, chloramphenicol 30 mcg�disc ; Er, erythromycin 15 mcg�disc ; Nv, novobiocin 30 mcg�disc ; Tr, trimethoprim 5.mcg�disc .

( )N. Ramesh et al. � Fitoterapia 72 2001 409�411 411

� �Uses in traditional medicine. As an antifertility agent 3 and in stopping menor-� �rhagia 4 .

Previously isolated constituents. No report.

Ž . � � ŽNew-isolated constituent. Luteoforol 3�,4�,4,5,7-pentahydroxyflavan 5�8 yield:.0.03% on dry weight .

Ž .Tested material. MeOH extract 17% and luteoforol.

� �Tested activity. Antibacterial activity by the agar-well diffusion method 9 .

Used microorganisms. Listed in Table 1.

Results. Reported in Table 1.

Conclusions. Both the methanol extract of B. crenulata stem bark and luteoforolshowed activity against all tested bacteria, even at the lowest concentrations testedŽ .approx. 1.6 and 0.25 mg�ml, respectively .

Acknowledgements

The authors sincerely thank the Director, Central Council for Research inAyurveda and Siddha, New Delhi, for providing experimental facilities.

References

� �1 Hooker JD. Flora of British India, Vol. 5. London: L. Reeve & Co, 1887:268.� �2 Gamble JS, Fischer CEC. Flora of the Presidency of Madras. London: Adlard & Son Ltd,

1925:1280.� �3 Mudgal V, Pal DC. Bull. Bot. Surv. India 1980;22:59.� �4 Viswanathan MB. Industry Meet cum Seminar on Biodiversity and Information on Medicinal and

Ž .Aromatic Plants. New Delhi: NISCOM, 1995:60 Abstract .� �5 Bate-Smith EC. Phytochemistry 1969;8:1803.� �6 Bate-Smith EC, Creassy LL. Phytochemistry 1969;8:1811.� �7 Styles ED, Ceska O. Phytochemistry 1975;14:413.� �8 Stich K, Forkmann G. Z. Natureforch. C. 1988;43:311.� �9 Perez C, Paul M, Bazerque P. Acta Biologiae et Medicine Experimentalist 1990;15:113.