antinociceptive and anti-inflammatory effects of the roots ethanolic
TRANSCRIPT
CADI AYYAD UNIVERSITYFACULTY OF SCIENCES SEMLALIA MARRAKECH
U.F.R: BIOLOGY AND HEALTHLABORATORY OF PHARMACOLOGY, NEUROBIOLOGY
AND BEHAVIOR
Antinociceptive and anti-inflammatory effects of the roots ethanolic extract of
Rubia peregrina
21st IBRO-ISN School of Neuroscience in Africa, Fayoum, Egypt
Supervisor:
Pr. A. CHAIT
Realised by:
Samira CHAIB
• 75% of african population still rely on traditional healing practices and medicinal plants for their daily healthcare needs.
•Morocco have an ancient and rich tradition in the field of herbal medicine, which makes the use of medicinal plants in treating diseases, an integrated part of Moroccan culture.
•One of such frequently Moroccan medicinal plants used is Rubia peregrina known as « FOUA ».
1.1 Introduction
1. Introduction 2. Materials and methode 3. Results and discussion 4. Conclusion
1. Introduction 2. Materials and methodes 3. Results and discussion 4. Conclusion and perspectives
1.2 systematic
Phylum: Angiosperms
Subphylum: Spermatophytes
Classe: Broadleaf
Order: Rubiales
Family: Rubiaceae
Gender: Rubia
1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion
Rubia peregrina
Liver disease Hypotension
Anemia Inflammation
1.3 Traditional use
1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion
1. Introduction 2. Materials and methodes 3. Results and discussion 4. Conclusion and perspectives
1.4 aims of study
Evaluation of the anti-nociceptive and anti-inflammatory effects of the root ethanolic extract of Rubia peregrina
1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion
1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion
Entrée d’eau
Sortie d’eauRéfrigérant
Matière à extraire
2.1 extraction methode
extraction dry continuous soxhlet
weight : 25-35g
LD photoperiodWater and food is provided Ad libitum
mals and femelles swiss albinos mice
1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion
2.2 biological materials
1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion
2.4 analgesic test (writhing test)
Evaluation of the sensitivity to nociceptive stimuli against chemical nociception
Writhing test
Fontenele, J.B., Viana, G.S., Xavier-Filho, J., de-Alencar, J.W., 1996. Anti-inflammatory and analgesic activity of a water-soluble fraction from shark cartilage. Brazilian Journal of Medical and Biological Research 29, 643–646
The nociceptive response is represented by the number of writhing performed by the animal tested
1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion
2.5 inflammatory test:
Chen, Q., 1993. Pharmacological Research Methods in Traditional Chinese Medicine. People’s Medical Publishing House, Beijing, p. 305.
treatment of mice with distilled water,
Diclofinac, or extract
applying a drop of xylene on both surfaces of the right ear
the animals were killed by cervical dislocation
both ears cut off, sized and weighed
Two hours
One hour
1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion
3.2 analgesic test (writhing test)
Controle
-
ASA25
0 mg/kg
500 m
g/kg
750 m
g/kg
Nb of writhing
Controle
-
Diclofin
ac
250 m
g/kg
500 mg/kg
750 mg/kg
1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion
3.3 inflammatory test:
1. Introduction 2. Material and methodes 3. Results and discussion 4. Conclusion
The results of the study demostrate that the administration of the ethanolic extract from R.P roots exhibits anti inflammatory properties. There was a significant and dose depondant inhibition of the acuteoedema, And a significant inhibition of pain in the writhing test.