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AO–{dH šQ¨ –g Ó~œYZ g›mMmaAbiotic Stress Management News

In this issueResearch Highlights• Quantifying CO2 and heat fluxes using Eddy Covariance• Gene expression and HSP polymorphism in poultry under

heat stress• Synthesis of nanostructured materials using fisheries waste• Aluminosilicates-based nanocomposite for alleviation of

multiple stressors• Development of polymeric product for gel and biofilm

formation and metals binding• Influence of chemicals on onion recovery under waterlogging• Improving NUE in sugarcane through fertilizer application

methods• Prototype of multipurpose machine for sugarcane ratoon crop• Bioregulator for recovery of hail damaged crops• Phenomics platform established• Low cost indigenous phenotyping tool• Breeding and seed production of Singhi• Impact of rearing temperature on tilapia• Plant bioregulators enhances water productivity for sorghum• Influence of deficit irrigation on growth, yield and fruit quality

of tomato• Genes and traits associated with drought tolerance in wheat• Virus-Induced Gene Silencing (VIGS) in soybean

New Initiatives• RNAi and VIGS in soybean• Screening of soybean for waterlogging tolerance• On-farm demonstration of improved rice production

technology• Protocol for assessing multiple heavy metals in aquatic water

bodies• Standardization of extraction protocol for crude biomolecules

from bacterial isolate• Standardization of quantitative detection of IAA and IBA• Integrated Agri-aquaculture• Nutriremediation for abiotic stress in fishes

Major Events• International Yoga Day• Distribution of HYV seeds of jowar to farmers under MGMG• Interaction Meeting with Scientists of ICAR-CCARI, Goa• Independence Day• Celebration of Ganesh Festival• Participation in Sports Meet• TSP Farmer’s Training Programme• {hÐXr MoVZm ›mg9 {hÐXr {Xdg #!"'

• Gandhi Jayanti and Swachh Bharat Oath• ITMU Cell activities

Personalia• Award / Recognition• Promotions• Joining• Superannuation

Editorial committeeDr. D. P. PatelDr. M. P. BrahmaneDr. Ajay Kumar SinghDr. Sachinkumar S. PawarDr. Neeraj KumarMr. B. GopalakrishnanMr. A. Balusamy

Compilation & Technical AssistanceMr. Praveen MoreMr. Madhukar Gubbala

Vol. 01 No. 01 An ISO 9001:2008 Certified Institute April to September, 2016

From the DirectorÊs Desk.....Greetings from ICAR-NIASM.

Food security of the ever increasingpopulation is being challenged by climatechange. Country expects that the agriculturalscientists should find out innovative solutions toabiotic stresses that are likely to occur morefrequently and with higher magnitude.Considering these challenges, ICAR-NIASM hasstarted basic and strategic research withinterdisciplinary approaches that integrates andcoordinates research on biotechnology, genetics, physiology, breeding, agronomy,cropping systems, horticulture, livestock and fisheries.

As Director of this new and unique Institute, there are many challenges andresponsibilities to create the best working atmosphere and research facilities so thatworking potential of existing young staff can be fully utilised. Institute witnessedmany important and significant events during the last six months. I am thankful toDr. Jagdish Rane, Head, SDSM, the Acting Director (till 11th September 2016), whofurther lead the development activities of the institute.

Institute Technology Management Unit initiated registration process for institutelogo as Trademark for product and services of the institute. This will help inmaintaining the quality of product and increased visibility of the institute technologyamong various stake holders in addition to Intellectual Property Right (IPR).

In addition to involvement in mandated research and institute developmentactivities, the staff of institute actively participated in many events such asInternational Yoga Day on 21st June 2016, Hindi Chetana Mas during 01-30th

September 2016, Hindi Diwas on 14th September 2016 and Swachhta Abhiyan onevery Wednesday during entire period.

Mera Gaon Mera Gaurav (MGMG) programme is gaining momentum and ourscientists are providing the requisite advice to the farmers of the adopted villages.

Highly favourable monsoon allowed timely planting of all the field experiments.As the monsoon was as per prediction to be normal, I hope and wish that most of thefarmers harvest good crop this year.

I thank the editorial team lead by Dr. D.P. Patel who has made tremendousefforts to include key highlights of the institute in this issue of the Newsletter. I alsoplace on record my thanks to all the staff members who have contributed to thisnewsletter. I extend my sincere thanks to Dr. T. Mohapatra, Secretary, DARE andDG, ICAR, and DDG (NRM), ICAR for their continued support to ICAR-NIASM. Iam very much confident that the first issue of Newsletter would be received wellby readers across different domains.

(Narendra Pratap Singh)

ICAR- National Institute of Abiotic Stress Management(Deemed to be University)

Malegaon Kh., Baramati - 413 115, Pune, Maharashtra, India

Research Highlights

Quantifying CO2 and heat fluxes from cropecosystems using Eddy Covariance method

One of the main objective of the institute is tocarry out basic and strategic research on climatechange. To achieve higher accuracy in climateprediction, modelers require reliable informationon land surface parameters such as sensible heatflux (energy that is emitted from soil and heats upthe overlying atmosphere), latent heat flux (energyconsumed in the process of evapo-transpiration)and greenhouse gas (GHG) fluxes over differentcrop surfaces across climatic zones.

Flux data processing protocols have beenstandardized and information generated onvarious flux dynamics. In chickpea, mean diurnalsensible heat flux rates during the initial, cropdevelopment, mid-season and late season stageswere 33.4, 23.0, 23.2 and 52.3 Wm-2, respectively.Variability in sensible heat fluxes could beexplained to the tune of 60-65% using either

remotely derived Enhanced Vegetation Index orCanopy Air Temperature Difference.

In case of CO2, it has been found that fordhaincha (green manure), wheat and soybeancrops, the seasonal mean of day time net flux ratesvaried between -5.2 to -7.6 µmoles m-2s-1 (negativesign indicating net uptake by crop ecosystem) andthat of night time rates ranged between 3.1 to 4.6µmoles m-2s-1 (positive sign indicating netemission).

Gene expression and HSP polymorphism inpoultry under heat stress

Heat stress is considered to be one of themajor concerns in poultry industry.Understanding the genetic basis of thermo-tolerance is crucial for successful poultryproduction and welfare. In the present study, abatch of commercial layer birds was reared undersummer heat stress for a period of three weeks.The birds were under moderate to severe heatstress during the entire experimental period. Theamplification of HSP gene polymorphic regionsi.e., HSP70 promoter region (364 bp) and 3’Untranslated region (434 bp) was achieved. Also,gene expression analysis was performed forHSP70 gene in the poultry birds under heat stress.

Monitoring of fluxes and biophysical parameters of cropecosystems

Experimental birds reared under summer heat stress

HSP70 expression in heat stressed poultry birds

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3Abiotic Stress Management News April to September, 2016

Research Highlights

Synthesis of nanostructured materials usingfisheries waste

Synthesis of nanoparticles is an importantcomponent of the rapidly growing research effortsin nanoscience and nanoengineering. One stepprocess has been developed for the synthesis ofsilver, zinc and selenium nanoparticles usingfisheries wastes and a patent application has beenfiled. The nanostructured materials have beencharacterized by SEM-EDAX, TEM and XRD. Thisinvention further relates to application of suchsynthesized bactericidal nanoparticles forcontrolling fish pathogens such as Aeromonashydrophila in aquaculture.

Characterization of nanostructured materials

Ag NPs in feed as wound healing agent in fish

Aluminosilicates-based nanocomposite foralleviation of multiple stressors

Novel nanoparticles and its role inenvironmental remediation is the subject ofextensive research. Nanomaterials haveincreasingly been used in water treatment becauseof economical and environmental viability andwider availability. Abundantly available zeolitesin quarries of Maharashtra and modifications ofthe surface and pores of zeolites make them

attractive candidates for various applications.Thirteen different forms of zeolites such asMesolite, Thaumarite, Okenite, Mordanite,Prehnite, Thomsonite, Gyrolite, Scolerite, Stilbite,Heulandite, Stellerite, Apophyllite and Ferreritewere subjected to trapping of silver nanoparticlesand native zeolites and zeolites trapped with silvernano particles were characterized by ICP-MS,which indicated the trapping of silvernanoparticles in zeolites. This has potentialapplication in alleviation of multiple stressors inaquaculture system viz., ammonia removal andbactericidal activity.

Bactericidal activity

Nanostructured materials

Different forms of zeolites


Research Highlights

Microbially derived polymeric product forgel formation and metals binding developed

The present invention relates to thedevelopment of low cost microbially derivedpolymeric product. The said product hasconsiderably high binding capacity withmonovalent and bivalent cations due to thepresence of multiple functional groups. Theproduct serves as an immobilizing matrix throughthe formation of biofilm and nurturing rootmicrobial colonization as it has high surface areaand longer retention time and protection frominhibitory compounds for maintenance of highmicrobial cell density and optimization ofmicrobial growth. The product acts as a goodgelling agent due to its water holding capacity. Ithas an application in gel formation for slowdelivery, microbial colonization through biofilmformation and the binding of toxic heavy metalsand other metallic cations including alkali andalkaline earth metals. A patent application hasbeen filed for this investigation.

Microbially derived polymeric product

Terrestrial weed endophytic halotolerant bacteria

Influence of application of chemicals ononion recovery under varying duration ofwaterlogging

An experiment was conducted to study theimpact of duration of waterlogging onphysiological manifestation and yield of late kharifgrowing onion var. Bhima Shakti. Six differentflooding duration viz., 0, 2, 4, and 6 days wereimposed at 15 days after transplanting of onion.Soil and foliar application of chemicals were tried.The results indicated that under normal conditionadditional nutrient application has significanteffect in increasing bulb weight and over all yieldsirrespective of mode of application over control.Waterlogging up to 2 days did not have muchinfluence on decreasing the yield of the plant. Theplants recovered well with the applied nutrients.However, the effect was predominant with foliarapplication of KNO3 and spermidine.

Waterlogging beyond four days drasticallyreduced the yield. The plant recovery after sixdays of pounding seems minimal. Nonetheless,with the application of chemicals, the yieldimproved at least 10-15%. Thick neck bulboccurrence was more as the days of waterpounding increased. This was significantlyreduced with foliar and soil application ofchemicals. The improvement was much betterwith foliar application of KNO3, spermidine andthiourea whereas sulphur and K application alongwith humic acid through soil performed betterthan Nitrogen (N) application. Soil application ofN negatively influenced some of the quality andbulb parameters. Sporadic flowering and thickneck bulb percentage was increased. TSS contentdecreased with the increase in waterlogging. Thisultimately decreased the pungency of the bulbwhich was reflected through decrease in pyruvicacid content. However, this could be negated withthe foliar application of KNO3.Bacterially derived polymer


Research Highlights

Improving nitrogen-use efficiency insugarcane through fertilizer applicationmethods under surface retention of trash

The colossal amount of trash (10-20 t/ha)generated after sugarcane harvest hampersparticularly fertilizer placement, therefore open-burning is a common practice in ratoon sugarcane.To address these issues for low yields of ratoonsugarcane, a stubble shaver, off bar cum fertilizerapplicator developed by IISR, Lucknow wasfurther upgraded at NIASM with inclusion oflarger capacity fertilizer box, robust powertransmission and root pruning mechanisms.Performance of this multi-purpose machinepopularly known as “SORF” (stubble shaver, off-bar, Root pruner cum fertilizer drill) machine wasdemonstrated and evaluated with ten on-farmtrials.

Surface retention of chopped trash and bandplacement of either recommended or double thedose of N as basal rather than recommended twosplits as basal and at earthing-up improved thecane yields and nitrogen uptake efficiency on anaverage by 16-22% and 9-11%, respectively overfarmer’s practices of trash burning andbroadcasting of fertilizers which led to increase innet profit of farmers by Rs. 27-50 thousand/ha.

Prototype of multi-purpose machine forsugarcane ratoon crop

Efforts have been made to develop a multi-purpose machine for trash chopping, off barring,root pruning, application of fertilizers and sowingof intercrops in sugarcane ratoon crop in a singlerun. The fine tuning of this prototype will offer aenvironmental friendly and practical solution totrash burning problem in sugarcane cultivation.

Demonstration of SORF machine

Multi-purpose machine


Research Highlights

Bio-regulator as an option for recovery ofhail damaged crops

Spraying of Bavistin + Copper oxychloridemixture @ 2 g/litre on sugarcane crop resulted ingood recovery of crop from the injury caused bythe hailstorm incidence happened at 60 days afterplanting. Moreover, application of additional 50kg N/ha just after the incidence along with eitherspraying of KNO3 (1.5%) or drenching of N (1.0%)and P2O5 + K2O (2.0% each) further boostedcrop growth and increased the cane yieldconsiderably.

Sugarcane crop on the day of hailstorm event

Sugarcane crop 180 days after the hailstorm event

ICAR-NIASM Phenomics facility

Phenomics platform established

A high-throughput plant phenomics facilityhas been established at the institute under NICRA.The facility will enable screening of large numbers

of plants from mapping, germplasm collectionbreeding and mutant populations for differentstresses such as drought, salinity and hightemperature. It will allow the application ofexperimental treatments such as water andnutrient stresses, and provide comprehensiveassessment of the responses of individualgenotypes through NIR, IR and visible imagebased procedures. Phenotyping protocols havebeen optimized for image acquisition and imageanalysis for crops such as soybean and mungbean.Phenomics platform was employed to identifymungbean genotype producing more biomasswith less water relative to Vaibhav (local check).

Images Utility Image aquisition

Visible Growth, senescence, shootarchitecture

225 pots

Infrared Canopy temperature, Indirectindicator of roots

Image aquisition-in 3

NR Plant water relations Processing- 1 hourOutput : 225 pots x7x600 column

Image Acquisition Image Processing Image Analysis


Research Highlights

Low cost indigenous phenotyping tool

Prototype for low cost indigenousphenotyping tool was developed to screen largenumber of genotypes in the field condition formeasurement of canopy temperature, groundcover and leaf senescence. This can be used astools for robust and rapid image based dataanalysis.

Image acquisition and analysis tool for field phenotyping

Hormonal injection in Singhi, fertilized eggs and larvae

Breeding and seed production ofHeteropneustes fossilis (Singhi) in WesternMaharashtra

Stinging catfish is a freshwater fish speciesnative to India. A protocol was standardized forlow cost breeding and seed production technology

Impact of temperature on Tilapia(Oreochromis mossambicus)

Tilapia is a fast growing fish which is able to

withstand varying environmental condition.

Juvenile tilapia was exposed to 25, 30 and 34°C

constant temperatures to understand the impact of

stress caused by high habitat temperature on fish

growth, white muscle cellularity and expression of

muscle genes, MyoD and Myostatin. At 60 day, fish

juveniles reared at 30°C grew significantly higher

than the 25°C and 34°C reared fish. The white

muscle histology revealed significantly higher

frequency of muscle diameter in the range of 25-

50 µm in fishes reared at 30°C. Expression of MyoD

was observed to be significantly higher at 34°C

whereas Myostatin gene expression did not vary

with change in rearing temperatures. The study

suggests that environmental temperature affect

the fish growth, muscle recruitment and

expression of genes responsible for muscle

formation.

in drought prone area of Maharashtra withoutsacrificing male brood of Singhi.


Research Highlights

Transverse section of tilapia (Oreochromis mossambicus) mosaicpattern of small and large white muscle fiber (100µm bar)

qRT-PCR quantification of MyoD and Myostatin mRNAexpression in white muscle of tilapia Oreochromis mossambicusreared at 25°C, 30°C and 34°C

Intensity of water stress on sorghum as affected by PBR’s atvarious quantities of applied water at 75 DAS

Plant bioregulators enhance waterproductivity of sorghum (Sorghum bicolor(L.) Moench)

Perceptive of the water stress on yieldformation is essential for planning of irrigation,natural resources and other mitigation strategies.Therefore, a field experiment (2014-16) wasconducted to evaluate the interactive effects ofexogenous application of plant bioregulators(PBR) and supplemental irrigation on yields andwater productivity (WP) of rabi sorghum (Cv.Phule Suchitra). Exogenous spraying of PBR’sincluded: 10 µM salicylic acid (SA), 100 mg L−1

sodium benzoate (SB), 500 ppm thiourea (TU),1.5% potassium nitrate (KNO3) applied at seedling

elongation (20 DAS), reproductive (50 DAS) andpanicle emergence (75 DAS) stages and control (nospray of PBR). Seven levels of irrigation water (IW)equaling to 0.95, 0.80, 0.65, 0.50, 0.35, 0.20 and 0.05times of the CPE (cumulative open panevaporation) were maintained using line sourcesprinkler system (LSS). It has been found thatapplication of PBR’s enhanced the grain yield,straw yield and water productivity of sorghum by6.8-18.5%, 5.7-14.7% and 0.39-1.41 kg m-3,respectively. Application of salicylic acid (10 µM)at higher irrigation levels and sodium benzoate(100 mg/L) and thiourea (500 ppm) at waterdeficit regimes was found best to alleviate theimpact of water stress. Overall PBR’s helped toreduce water use by 25-49%. The integrated use ofbio-regulators with deficit irrigation cansubstantially enhance the productivity vis-a-visprofitability from cultivation of rabi sorghumunder water scarce conditions.


Research Highlights

Influence of deficit irrigation strategies atdifferent growth stages on growth, fruityield and physio-chemical quality of tomato(Lycopersicon esculentum Mill.)

The regulated deficit irrigation (RDI0.8 i.e0.8xETc) did not affect the marketable fruit yield(MFY) as compared with the full irrigation (FI 78.0Mg ha-1). The water productivity of 19.2 kg m-3

was the maximum under RDI0.8. The MFY wasimproved by 4% with DI0.6 at vegetative stagewhile DI0.6 at flowering stage showed little effectand a decline of 7% was monitored with DI0.6 atfruiting stage. The DI0.6 applied at either of twostages vegetative & flowering, flowering &fruiting and vegetative & fruiting resulted in 14-18% decline in MFY. The crop was able to tolerateinterruptions of irrigation for 15 days at growthstages and the decline in yield was only 3-7%, thehighest being at fruiting stages.

Fruit quality and functional food propertiesimproved with DI. Colour (a/b* ratio) and theconcentrations of vitamin C increased by 82 and70% with RDI0.6 compared to FI. With-holdingirrigation during fruit development inducedcolour development (52%), vitamin C content(16%) and recorded better lycopene content (10.2mg/100 g). The total phenolics and flavonoidsranged between 16 and 42 mg gallic acidequivalent/100 g and 4–10 mg catechinequivalent/100 g respectively with higher valuesrecorded under DI at fruiting stage. Antioxidantactivity (AOA) tested with different in vitro assaysrevealed the positivity of DI on improvement ofbioactive compounds. Superoxide dismutaseactivity was significantly higher in DI treatmentsat fruiting stage compared to catalase activity.

Genes and traits associated with droughttolerance in wheat

To assess genetic variation in droughttolerance, 60 wheat genotypes collected from

ICAR-National Bureau of Plant Genetic Resources,New Delhi, were screened under field conditions.Traits such as stay green feature measured bychlorophyll index using SPAD meter, shootbiomass, chlorophyll fluorescence and yieldrelated attributes were evaluated. Geneticvariation was also measured in Relative WaterContent (RWC), CO2 assimilation rate andtranspiration rate under well-watered and waterstressed condition. Based on these parameters IC-549394 was identified as a promising genotypewith high adaptability to soil moisture deficit. Thisgenotype had deeper root system, more roots andprofuse root branching. The superiority of thisgenotype over local check could be attributed tohigher expression of CBF4 and CDPK19 genesboth under well-watered and water stressedconditions.

Comparison of root traits measured in vitro in promisingwheat genotype and local variety HD-2189

Grain weight measured in promising wheat genotype relativeto local variety HD-2189


Research Highlights

HD-2189

Root system architecture of HD-2189 and IC-549394

qRT-PCR analysis of CBF4 gene in HD-2189 and IC-549394

IC-549394

qRT-PCR analysis of CDPK gene in HD-2189 and IC-549394

Virus induced gene silencing in soybean

Virus induced gene silencing (VIGS)technique was successfully used to investigate therole of candidate genes associated with droughttolerance in soybean. Selected soybean genotypesincluding locally well adapted cultivar JS-335, and

NRC-37 were used in the experiment to silenceEthylene-In-sensitive2 (EIN2) gene and to validateits role in drought tolerance. EIN2-silencedsoybean plants exhibited a drought-tolerantphenotype characterized by low levels oftranspiration and enhanced expression ofdrought-responsive genes. In addition, EIN2silenced soybean plants exhibited increasedexpression of SA responsive PR1 gene, reducedexpression of JA responsive PR3 gene but had highlevel of ADP Riboxylation factor and WRKY-39gene indicating significance of these genes inimparting drought tolerance.

Silencing of EIN gene and expression profiling of genesassociated with water stress tolerance in soybean

Mock Vector alone EIN-silenced

Phenotype of mock, vector alone and EIN gene silencedsoybean plants

n n n

M V sEIN2 M V sEIN2

M V sEIN2

GmWRKY-39GmARFβ-tubulin

GmPR1GmPR3β-tubulin

β-tubulin


New Initiatives

RNAi and Virus-Induced Gene Silencing(VIGS) in soybean

Experiments were initiated to enhancedrought and heat stress tolerance in soybean andidentification and functional characterization ofmaster genes associated with drought and heatstress tolerance mechanisms in soybeanemploying RNAi and VIGS approaches.

Screening of soybean for waterloggingtolerance

A set of more than 100 germplasm lines ofsoybean including important high yieldingvarieties were collected from IISR, Indore forevaluating waterlogging tolerance. A fieldexperiment has been conducted to screen thetolerant varieties/lines where waterloggingtreatment was imposed at vegetative andreproductive growth stages. Responses of plantsto waterlogging was evaluated based on variousparameters such as depletion of chlorophyllcontent, plant biomass, yield and yield attributesand changes in root anatomy.

Waterlogging treatment at vegetative stage

On-farm demonstration of “Improved riceproduction technology” under TSP

More than 100 tribal farmers of variousvillages from Navapur Taluka of Nandurbar (MS)

were selected under TSP for on-farmdemonstration of improved rice productiontechnology suitable for the area. HYV seeds of ricevar. Indrayani and Phule Samruddhi weredistributed to the farmers. Fertilizer briquetteswere supplied to all the beneficiaries. On- farmtraining was also given to the farmers for properadoption of the technology.

Distribution of fertilizer briquettes to farmers

Protocol for assessment of heavy metals inaquatic bodies

ICP-MS based protocol has been standardized

for assessment of multiple metals such as Li, Be,

Na, Mg, Al, K, Ca, Ti, V, Cr, Mn, Fe, Co, Ni, Cu,

Zn, Ga, As, Se, Rb, Sr, Cd, Cs, Sn. Sb, Hg, Pb and

Bi in the surface water, soil sediments and fish

tissue samples viz. gill, liver, kidney, muscle and

gonad. In addition, enzyme based protocols have

been standardized as biomarkers for monitoring

heavy metals contamination in aquatic water

bodies.

Standardization of extraction protocol forcrude biomolecules from bacterial isolateusing XAD-16

XAD-16 based method has been standardizedfor extraction of microbially derived biomoleculesusing UHPLC.


New Initiatives

XAD16 based method for extraction of microbial metabolite

Standardization of rapid method forquantitative detection of IAA and IBA

Bacterial isolates obtained from differentsaline soil samples were evaluated for their plantgrowth promotional traits in terms of IAA,siderophores under simulated drought andsalinity stress. UHPLC based methods for rapiddetermination of bacterially elaborated biomole-cules were developed, mainly for plant-growthhormones. The methods for rapid detection andquantification of IAA, IBA, GA3, Zeatin and ABAwere standardized. Using these methods, presenceof all the above hormones was successfullydetermined in the organic extracts of the isolates.The average frequency of plant hormones in theorganic extracts of the isolates was IAA > GA3 >IBA > ABA; while the abundance of the hormonesfound to be IAA > IBA > GA3 > ABA in the testedisolates. Further, the method for metaboliteprofiling has also been developed to resolve the

Integrated agri-aquaculture

ICAR-National Institute of Abiotic StressManagement, Baramati has successfullyimplemented improved technology interventionsin field and horticulture crops, dairy, poultry and

UHPLC chromatogram for biomolecules

fine aspects of bacterial metabolic diversity underdifferent stress situation. This method successfullyresolved the differences within the organic extractsof single isolate cultivated under varying stresses.

Demonstration of integrated agri-aquaculture

HPLC profile for standards of plant growth hormones (IBA,IAA, Zeatin and ABA)


New Initiatives

fish farming, and integrated farming in variousvillages of Navapur tehsil in Nandurbar district ofMaharashtra for improving the livelihood ofresource poor tribal farmers under Tribal Sub-Plan(TSP).

Development of zinc nanoparticles formitigation of abiotic stress in fishes

Zinc nanoparticles (Zn-NP) have beendeveloped from fisheries waste and it wasincorporated as feed supplements for novel feed

formulation to mitigate abiotic stress. A 75 daysexperiment was conducted to evaluate themitigating role of Zn-NP against abiotic stresssuch as heavy metal and high temperature. It wasobserved that fish growth was noticeably (p<0.01)higher in Zn-NP fed group and also cellularmetabolic stress has been reduced. Theimmunological status of the fish was alsoimproved significantly after bacterial challenge.Overall results indicated that dietary Zn-NP canconfer protection to fish against abiotic stress.

Major events

International Yoga Day

International Yoga Day was celebrated onJune 21, 2016 by following the Common YogaProtocol from Ministry of AYUSH, Government ofIndia. About 40 staff members participated in theYoga Day celebration at 6:00 AM. The programmewas started with the display of speech byHonorable Prime Minister Shri Narendra Modidelivered at the 69th session of United NationsGeneral Assembly on September 27, 2014. TheYogic Practice was started with a prayer toenhance the benefits of practice. Dr. PravinTaware, Technical Officer instructed the sessionthat included Yogasanas (Tadasana, Vrksasana,

Pada-hastasana, Ardha-chakrasana, Trikonasana,Bhadrasana, Ardha-ustrasana, Bhujangasana,Shalabhasana, Pavanmuktasana and Savasana),Pranayamas (Kapalbhati, Anulom-vilom andBhramari) and Dhyana. The session concludedwith Sankalpa for healthy, peaceful and joyful self.

Distribution of HYV seeds of jowar tofarmers under MGMG

A HYV seed of jowar Cv. Phule Suchitragrown in experimental plots of the institute wasdistributed to the selected farmers of the adoptedvillage Nimbodi and Lakadi of Indapur Taluka,Pune under MGMG on 30th July 2016. Complete


Major events

package of practices for cultivation of jowar wasalso imparted to the farmers. A large number offarmers showed keen interest in cultivation ofjowar. Most of the farmers in these villages arecultivating traditional variety of jowar i.e,Maldandi.

Interaction Meeting with Scientists of ICAR-CCARI, Goa

An interaction meet between scientists ofICAR-NIASM, Baramati and ICAR-CCARI(Central Coastal Agriculture Research Institute),Goa was organized on August 1-2, 2016. It was afollow up action to the proceedings of NRMdivision`s Director`s Meet held at ICAR-CCARI,Goa during 11-12 April, 2016. In the meeting,Director, ICAR- NIASM presented an appraisalreport on research facilities and ongoing researchwork of ICAR-NIASM to be a potential partner forresearch collaboration with ICAR-CCARI, Goa.Director, ICAR-CCARI, Goa in his presentationhighlighted potential area where ICAR-CCARIneed to be collaborating with ICAR-NIASM forutilization of available facilities at both theinstitutes to address abiotic stresses in agriculturefor the benefit of poor stake holder i.e. farmers.Thereafter scientists of ICAR-CCARI, Goadelivered talk in their respective area of researchand also insight into most suitable themes wherethey wish to collaborate with ICAR-NIASM. Athrough discussion was held to conclude thesession and the point for potential collaborationwere suggested. All the scientists of ICAR-NIASM,Baramati participated in the meeting. Dr.Narendra Pratap Singh in his concluding remarksappreciated the way by which the meetingorganized and stressed upon the frequent visit ofscientist from ICAR- NIASM to ICAR-CCARI, Goaand vice- versa to exchange the ideas andutilization of the facilities available at both theinstitute.

Independence Day Celebration

69th Independence day was observed on 15th

August 2016 in the institute and National Flag washoisted by the Director. All the staff membersalong with their family participated in the event. Anumber of games were also organised on the day.

Ganesh Festival Celebration

Keeping in tune with the great tradition ofMaharashtra, the institute staff celebrated GeneshaChaturthy during 5th to 9th September 2016.


Major events

Participation in ICAR Zonal Tournament2016 - Western Zone

A fourteen member squad namely- Dr.Yogeshwar Singh, Dr. K.K. Meena, Dr. D.D.Nangare, Dr. G.C. Wakchaure, Dr. R.L.Choudhary, Dr. Neeraj Kumar, Dr.Prashantkumar S. Hanjagi, Mr. Paritosh Kumar,Mr. P.R. Chahande, Mr. R.S. Ghopane, Mr. SunilPotekar, Mr. Lalit, Mr. Pravin More and Mr.Aniket More from ICAR-NIASM participated inthe ICAR Zonal Tournament 2016 - Western Zoneheld at ICAR-NRCC, Bikaner during September24-27th, 2016. All fourteen contingents participatedin various games namely Volleyball, Kabaddi,Basketball, Football, Table tennis, Badminton,Chess, Shotput, Discuss throw, Carom and 200,400, 800 and 1500 m Race in the sports meet. Dr.Prashantkumar S. Hanjagi received three medalsviz., Gold, Silver and Bronze in 200, 400 and 1500m race, respectively. Kabaddi and football teamreached up to semi-final.

TSP Farmer’s Training Programme

ICAR-National Institute of Abiotic StressManagement (NIASM), Baramati is undertakingactivities related to improved technologyinterventions in field crop, horticulture, livestock,poultry, fisheries and integrated farming invarious villages of Navapur Tehsil in Nandurbardistrict. In this regard, recently Dr.NarendraPratap Singh (Director, ICAR-NIASM), Dr. K.K.Krishnani (Chairman-TSP), TSP-members Dr. N.P.Kurade, Dr. D.P. Patel, Dr. A.V. Nirmale and Dr.Neeraj Kumar visited Navapur for reviewing theon-going TSP activities. Training programme on“Nutritional management in livestock andfisheries for livelihood improvement of tribalfarmers” was conducted at Visarwadi andNavapur on 17-18th September 2016. About 400farmers from various villages of Navapur Tehsilparticipated and benefitted.

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Major events

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Gandhi Jayanti and Swachh Bharat Oath

Gandhi Jayanti was observed on 2nd October2016 and an awareness programme on SwachhBharat Abhiyan was organized. The staff of theinstitute along with villagers and members ofGramsabha, Malegaon Khurd took SwachhataShapath.


Major events

ITMU Cell activities

Recruitment of SRF

• One SRF was recruited under ITMU Cell in themonth of September 2016.

Registration of Trademark

• Initiative was taken and necessary formalitieshave been completed for registration ofinstitute logo as Trademark under two classesi.e, 31 & 44 which covers product and services.

Workshops/Seminar/Symposia/Trainingattended

• Dr. K.K.Krishnani, Head (SESM) participatedin 3rd International IUPAC Conference on“Agrochemicals Protecting Crops, Health andNatural Environment” at IARI, New Delhiduring 6-9th April, 2016 and Felicitated to chairtechnical session of invited talks and alsodelivered resource lecture on Biotechnologicaltools for enhancing biodegradation ofpersistent organic compounds.

• Dr. Neeraj Kumar, Scientist (Fish Nutrition &Biochemistry) attended 3rd International IUPACConference on “Agrochemicals protectingCrops, Health, & Natural Environment” atIARI, New Delhi during 6-9th April, 2016.

• Dr. S.K. Bal, Principal Scientist (Agrometeoro-

logy) & I/C Head (SASM), participated anddelivered an ignited presentation on “FutureThrust and Strategies of AgrometeorologicalResearch in India” in a brainstorming sessionorganized at ICAR-CRIDA, Hyderabad on 29th

April, 2016.

• Dr. Jagadish Rane, Head (SDSM), participatedin Interactive Meet of Directors & FinanceOfficers of ICAR Institutes (West Zone) atICAR-CIFE, Mumbai on 6th May, 2016.

• Drs. S.K. Bal, Jagadish Rane, K.K. Krishnaniattended brainstorming session on 'AbioticStress Management with Focus on Drought,Flood and Hailstorm at NASC, New Delhiduring 21-24th May, 2016.

• Dr. Jagadish Rane, Head (SDSM), attended 86th

Board of Studies meeting in Agril. Botany,MPKV, Rahuri on 25th May, 2016.

• Dr. K.K. Krishnani, Head (SESM) deliveredresource lecture on “Bioremediation ofchemical and microbial contaminants inaquaculture – Application of molecular andnanotechnological tools at Central Institute ofFisheries Education, Mumbai on 25th May 2016.

• Dr. Jagadish Rane, Head (SDSM), signed aMoU with IGKV, Raipur for collaborativeresearch including students at Raipur during30-31st May, 2016.

• Dr. K.K.Krishnani, Head (SESM) participatedin Pre-RMP Management DevelopmentProgramme on Leadership development atNational Academy of Agricultural ResearchManagement, Hyderabad, during 7-18th June2016.

• Dr. Jagadish Rane, Head (SDSM), attendedRAC meeting in NIBSM, Raipur during 11-13th

July, 2016.


Major events

• Mr. Rajkumar, Scientist (AgriculturalEntomology) attended three monthsprofessional attachment training at ICAR- IIHR,Bangalore during 7th June to 6th September 2016.

• Dr. Jagadish Rane, Head (SDSM), chaired theplenary session of Annual Meeting ofMaharashtra Grape Growers Association atPune during 26-28th August, 2016.

• Mr. Paritosh Kumar, Scientist (EnvironmentalSciences) attended professional attachmenttraining at ICAR- IARI, New Delhi during 22nd

May, 2016 to 6th September, 2016.

• Mrs. Sravanti Bandela, Scientist (Spices,Plantation & Medicinal & Aromatic Plants)attended three months professional attachmenttraining at Dr. Jayashankar, Telangana StateAgricultural University, Hyderabad during30th May, 2016 to 29th August, 2016.

• Dr. S. K. Bal, Principal Scientist (Agrometeoro-logy) & I/C Head (SASM), attended Ist PIs’meeting of DST sponsored BDA-HSRS project(Theme: Agriculture) organized by ICAR-NBSSLUP, Nagpur on 21st June, 2016.

• Mr. Pravin More, Technical Assistant(Computer) attended training on“Implementation of NIC's e-procurementsolution through CPP portal” at ICAR-NDRIKarnal during 21-22nd July, 2016.

• Mr. Pardeep Kumar, Assistant attendedtraining on “Implementation of NIC's e-procurement solution through CPP portal” atICAR-NDRI Karnal during 21-22nd July, 2016.

• Dr.K. K. Krishnani, Head (SESM) participatedin Workshop at Cochin University of Scienceand Technology, Kochi, India during 23-24th

July, 2016.

• Dr. K. K. Krishnani, Head (SESM) deliveredinvited / resource lecture on “Recent advancesand molecular perspectives of microbialbioremediation in aquatic environment” atCentral Institute of Freshwater Aquaculture,Bhubaneshwar, India on 30th July, 2016.

• Mr. Sunil Potekar, Technical Assistant(Agrometeorology) attended training on “Agrometeorological Data Collection, Analysis andManagement at ICAR-CRIDA, Hyderabadduring 25th July 2016 to 6th August, 2016.

• Dr. Mahesh Kumar, Scientist (Plant Physiology)attended National conference on “Bringing SelfSufficiency for Pulses in Eastern India” at BiharAgricultural University, Sabour during 05-06th

August, 2016.

• Dr. K.K.Krishnani, Head (SESM) deliveredinvited/resource lecture on “Integratedapproach for assessment and remediation ofcontaminants in soil, water and related aquaticenvironment” in DST sponsored InspireInternship Camp at Pt Ravishankar ShuklaUniversity, Raipur during 12-14th August, 2016.

• Mr. V. Rajagopal, Scientist (Soil Chemistry,Fertility & Microbiology) attendedInternational conference on “Agriculture, FoodScience, Natural Resource Management andEnvironmental Dynamics: The Technology,People and Sustainable Development” atBCKV, Kalyani, West Bengal during 13-14th

August, 2016.

• Dr D.D. Nangare, Scientist SS (Soil & WaterConservation Engineering) participated intraining programme on “Analysis ofexperimental data” at NAARM, Hyderbadduring 18-23rd August, 2016.


Major events

• Dr. Jagadish Rane, Head (SDSM), attended 24th

Meeting of Regional Committee No. VIIorganized at Goa during 8-9th September, 2016.

• Mr. V. Rajagopal, Scientist (Soil Chemistry,Fertility & Microbiology) attended State levelseminar on “Development in Soil Science:Climate change and its influence in naturalresource management” organized at BSKKV,Dapoli during 22-23rd September, 2016.

• Mr. Pravin More, Technical Assistant(Computer) attended training on Cybersecurity at ICAR- IASRI, New Delhi during 28th

September to 5th October, 2016.

• Mr. Santosh Pawar, Technical Assistant(Electrical) attended training on “ProjectManagement” organized by National Councilfor Training and Social Research (NCTSR),New Delhi at Goa during 21-23rd September,2016.

Personalia

Award/ Recognition

• Dr. K.K. Krishanani, Head (SESM) was electedat NAAS Fellow at the National Academy ofAgricultural Sciences, New Delhi on 4-5th June2016, for his outstanding contribution on“Bioremediation of aquatic contaminants usingmolecular tools”.

• Dr. Neeraj Kumar, Scientist (Fish Nutrition &Biochemistry) received best publication of theYear 2015 Award on June 11, 2016 from ICAR-CIFE, Mumbai.

Promotions

• Smt. Purnima Shashikant Ghadge promoted toAssistant Administrative Officer on 17-06-2016.

• Dr Pravin Taware promoted to Sr. TechnicalOfficer (T6).

Joining

• Mrs. Bandela Saravanti joined as Scientist(Spices, Plantation & Medicinal & AromaticPlants) on 7th April 2016.

• Mr. Rajkumar joined as Scientist (AgriculturalEntomology) on 11th April 2016.

• Mr. Paritosh Kumar joined as Scientist(Environmental Sciences) on 11th April 2016.

• Dr. Narendra Pratap Singh joined as Director ofthe ICAR-NIASM on 12th September 2016.

Superannuation

• Dr. P. S. Minhas, Director, ICAR- NIASMsuperannuated on 30th April, 2016.

• Mr. Milind. S. Bhatkar, Administrative Officeropted for VRS and superannuated on 21st

September 2016.

n n n

Published byDr Narendra Pratap Singh, Director, ICAR-NIASM, Malegaon, Baramati- 413 115, Pune (MS), India.

Phones : (02112) 254057, 254058 • Fax : (02112) 254056 • Email: [email protected]: www.niam.res.in

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