apaf-1 caspase-9 cyt c control sulindac control e ly294002 control trail thapsigargin...
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Apaf-1
Caspase-9
Cyt c
control
control
sulindac
control
E LY294002
control
control
TRAILthapsigargin
HT-29 Chondrocyte
control
SIN-1
RAW 264.7
IP: AChE
C
Supplementary Fig. S1
A B
IP : Apaf-1WB: AChE
IP: Caspase-3WB: AChE
IP: Caspase-9WB: AChE
IP: c-IAP-1WB: AChE
IP: c-IAP-2WB: AChE
IP: Cyt cWB: AChE
E40 - + E40 - +tre
ated
Thapsigargin2.5 M, 24 h
HT-29
Sulindac1 mM, 48 h
E4040 h
LY294002100 M, 24 h
TE671
BMMC
U373MG
E5024 h
E4024 h
SK-MEL5
E1048 h
RAW 264.7
SIN-11 mM, 24 h
IL-3 deprivation
Malme-3M
E5024 h
TRAIL100 MOI, 72 h
Chondrocyte
SNP1.5 mM, 24 h
-actin
-actin
-actin
-actin
-actin
-actin
-actin
-actin
-actin
-actin
-actin-actin
cont
rol
cont
rol
treat
ed
E40 si Cyt c
Cyt
cH
oech
st
--
+-
-+
++
A
Supplementary Fig. S2
Bsi Cyt c
0 8 16 24 48 h
ChondrocyteTRAIL 100 MOI
HT-29Thapsigargin 2.5 M
Apaf-1
Apaf-1
IP: AChE
IgG
IgG
105
50
105
50
IP: Apaf-1WB: Apaf-1
IP: Apaf-1WB: Apaf-1
IgG
IgG
PC
IP: AChE
SK-MEL-5E10
RAW 264.7SIN-1 1 mM
0 8 16 24 48 h
105
50
105
50
Apaf-1
Apaf-1
IgG
IgG
IgG
IgG
si Cyt c
IP: Apaf-1WB: Apaf-1
IP: Apaf-1WB: Apaf-1
PC
Supplementary Fig. S3
IP : Cav-1
E40 0 8 16 24 48 72 h
AChE
IgG50
80
A B
si Cyt c
E40 0 8 16 24 48 72 h
IP: Cav-1
IgG
AChE
50
80
C
D
IP: Apaf-1WB: AChE
E50 (LNCaP) E10 (PC-3)0 24 48 h 0 24 48 h
IP: Cav-1WB: AChE
IP: Cyt cWB: AChE
IP: Cyt cWB: Apaf-1
IP: Apaf-1WB: AChE
IP: Cav-1WB: AChE
IP: Cyt cWB: AChE
IP: Cyt cWB: Apaf-1
IP: AChEWB: AChE
IP: AChEWB: AChE
IP: AChEWB: AChE
IP: Apaf-1WB: Apaf-1
IP: AChEWB: AChE
IP: AChEWB: AChE
IP: AChEWB: AChE
IP: Apaf-1WB: Apaf-1
PC PC
IgG IgG
IgG IgG
IgG IgG
IgG IgG
IgG IgG
IgG IgG
IgG IgG
IgG IgG
E40 0 8 16 24 48 h
105
50
50
15
IgG
IgG
Apaf-1
Cyt c
IP: Cav-1
IP: Apaf-1WB: Apaf-1
IP: Cyt cWB: Cyt c
IgG
IgG
PC
E
IP: Cav-1
MCDE40
-+
1/12+
1+
2+
24h+
--
AChE
IP: Apaf-1
AChE
Cyt c
IgG
IgG
IgG
50
25
IP: Cyt c
50
80
50
80