appendices - springer978-3-662-04129-1/1.pdf · also used for pumping dye lasers with 514 nm light....

Appendices

Appendices

BIRGIT MECHTOLD AND ANDREAS RADBRUCH

Glossary

Absorption

Acquisition Alignment microscope

The taking up of light, sometimes only of certain wavelengths (absorption wavelength) and transforming it either to other forms of energy or to light of other wavelenghts (fluorescence). The recording of data in flow cytometry. Serves to monitor the nozzle, flow stream, and breakoff point, as illuminated by a stroboscope in drop drive frequency, to measure distances (=time) between laser interception and breakoff point, and to calculate the drop delay.

Anticoincidence If cells pass the point of analysis at too short a distance, both cells can circuit give an artificial signal, or the second cell not be measured correctly

because the system is not yet ready (analysis abort). For cell sorting, cells following each other too closely to be packed in to independent droplets, or differentially sorted droplets, may be included for the sake of recovery, or not, for optimal purity (sort abort).

Allophycocyanin A fluorescent phycobiliprotein isolated from the cyanobacterium (APC) Anabena variabilis. The peak of fluorescence emission is 660 nm; the

Argon laser

Auto-fluorescence Bandpass filter (BP) Barrier filter Channel

Cluster designation (CD)

peak of absorption is close to 650 nm. For flow cytometry it can be excited by dye lasers (ca. 560 nm) or helium/neon lasers (632 nm). The most frequently used illuminating light source in flow cytometry. Useful for UV excitation of Hoechst dyes (DNA of live cells, bro-modeoxyuridine/Hoechst quenching) and indo-l measurements: useful for excitation of most immunofluorescence dyes, propidium iodide (DNA), and Fluo-3 (Ca flux) with 488 nm blue light: sometimes also used for pumping dye lasers with 514 nm light. Endogeneous fluorescence, a problem with fibroblasts and fixed cells, can be compensated by measuring it as an independent parameter. An optical filter transmitting a small spectral range of light.

An optical filter blocking a small spectral range. The numeric value of a parameter, i.e. the digitalized signal intensity. The range is 256 - 1024 channels. The nomenclature for leukocyte surface antigens. Listed in : Leucocyte typing IV by W. Knapp, Oxford University Press, 1989 and a poster by Janssen Biochimica, Belgium.

330 BIRGIT MECHTOLD AND ANDREAS RADBRUCH

Compensation Contour plot

Coefficient of variation (CV) Dichroic mirror (DC) Dot plot

Drop delay

FACS Fluorescence

Forward scatter (FSC) Gate

HeCd laser Live gate Longpass (LP) MACS Mean

Median Orthogonal light scatter Peltierelement

Photomultiplier tube (PMT) Propidium iodide (PI) Piezoelectric

Shortpass (SP)

Sides catter (SSC) Threshold trigger

The subtraction of cross-talk between photodetectors. A plot showing two parameters coordinated, Contour lines circumventing areas containing equal densities of events in linear, log, or probability fashion. The standard deviation (channel number) divided by the mean fluorescence of the fluorescence distribution, x 100. A mirror which transmits and reflects light according to wavelength.

A plot showing two parameters coordinated, with each event plotted as a dot according to the intensity of the parameters. Distance (time) required by a cell to fly from the point of analysis to the breakoff point, i.e. from analysis to sorting, in flow-in-air sorters. Fluorescence-activated cell sorting The property of molecules to absorb light of a particular wavelength and emit light of a longer wavelength. Light scattered by a cell in the direction of the excitation light beam as the cell passes the beam. Intensity thresholds defining a region of intensity of a particular flow cytometric parameter, used for further analysis or sorting. Helium-cadmium ion laser. A gate set for recording of cells. An optical fIlter blocking all light shorter than a certain wavelength. High-gradient magnetic cell sorting The sum (arithmetic, linear amplification» or product (geometric, logarithmic amplification) of all signals, divided by the number of signals. The channel at which 50% of cells lie below and 50% above. Light scattered to the side (see forward scatter).

An electronic element generating a temperature gradient from current. Useful for controlling temperature in flow, e.g. to cool cells. A photodetector, with photons generating a cascade of electrons to

generate a measurable current. A red-fluorescent dye staining DNA; a marker for dead cells because it is excluded quantitatively by live cells. An electronic element converting electronic frequencies into mechanical vibrations of the same frequency. Used to generate a stable breakoff point and a defined number of droplets in flow-in-air systems. An optical fIlter that blocks all light of wavelengths longer than a particular wavelength. Light scattered to the side as the cell passes through the laser beam.

The minimum signal intensity to trigger data processing.

Suppliers

Suppliers

Aber Instruments Ltd. Science Park Aberystwyth SY23 3AH, United Kingdom Phone: +44.1970.636300 Fax: +49.1970.615455 E-mail: [email protected]

Products

Flow Cytometer systems:Microcyte

Internet: http://www.aber-instruments.co.uk/index.htm

Aldrich Chemie GmbH see SIGMA-Aldrich Chemie GmbH

American Type Culture Selection (ATCC) 12301 Parklawn Drive Rockville, MS 20582 - 1776, U.S.A. Internet: http://www.atcc.org

Bachem Biochemica GmbH Haberstr. 6 D-69126 Heidelberg Phone: +49.6221.3305-0 Fax: E-mail: Internet:

+49. 6221. 3305-99 [email protected] http://www.bachem.com

Bachem Bioscience INC. 3700 Horizon Drive King of Prussia, P A 19406, U.S.A. Phone: +1. 610. 239-0300

l. 800. 6343183 Fax: +1.610.239-0800 Internet: http://www.bachem.com

Bachem, Feinchemikalien AG Hauptstr. 144 CH-4416 Bubendorf, Switzerland Phone: +4l. 6l. 9312-333 Fax. : +4l. 6l. 9312-333 Internet: http://www.bachem.com

Culture cells

Biochemicals

Biochemicals

Biochemicals

Appendices 331


Balzers Process Systems GmbH P.O. Box 1000 FL- 9496 Balzers, Liechtenstein Phone: +41. 75. 388-4111 Fax: +41. 75. 388-5426 Internet: http://www.balzers.com

Becton Dickinson GmbH Tullastr. 8-12 D-69126 Heidelberg Phone: +49. 6221. 305-0 Fax: +49. 6221. 305-216 Internet: http://www.bdfacs.com

Becton Dickinson Immunocytometry Systems (BDIS) 2350 Qume Dr. San Jose, CA 95l31-1807, U.S.A. Phone: + 1. 408. 432-9475

1. 800. 223-8226 Fax: + 1. 408. 954-2347 (BDIS) Internet: http://www.bdfacs.com

Bio-Rad Laboratories GmbH Heidemannstr. 164 D-80939 Miinchen Phone: +49.89.31884-0 Fax: +49. 89. 31884-100 Internet: http://www.biorad.com

Boehringer Ingelheim GmbH Bioproducts D-55216 Ingelheim Phone: +49.6132.773582 Fax: +49. 6l32. 776601 Internet: http://www.bi-bioproducts.de

Biickmann GmbH Technische Gewebe und Maschinen KonstantinstraBe 46 D-41238 Monchengladbach Phone: +49.2166.80023 Fax: +49. 2166. 80023

Interference Filter

Flow Cytometer systems: F ACSAnalyzer F ACSCalibur FACScan FACSort FACStar FACStrack FACSVantage and Antibodies

Flow Cytometer systems: F ACSAnalyzer F ACSCalibur FACScan FACSort FACStar FACStrack FACSVantage and Antibodies

Life Science research products Flow Cytometer systems: Bryte-HS

Biochemicals and Antibodies

Gauze

Calbiochem Novabiochem GmbH Postfach 1167, Hoechst Lisztweg 1 D-65796 Bad Soden Phone: +49. 6196. 63955

0800.693-1000 Fax: +49. 6196. 62361

Antibodies, Biochemicals and dyes

E-mail Internet:

calbiochem [email protected] http://www.calbiochem.com

Calbiochem Novabiochem Corporation Antibodies, Biochemicals and dyes P. O. Box 12087 La Jolla, CA 92039-2087, U.S.A. Phone: + 1. 619.450-9600

1. 800. 854-3417 (Calbiochem) 1. 800. 228. 9622 (Novabiochem)

Fax: + 1. 619. 453-3552 1. 800. 776. 0999

Internet: http://www.calbiochem.com

Caltag Laboratories 1849 Bayshore Boulevard 200 Burlingame, CA 94010, U.S.A. Phone: +1.650.652-0468

1. 800.874-4007 Fax: +1.650.652-9030 E-Mail: [email protected] Internet: http://www.caltag.com

Coherent (Deutschland) GmbH Dieselstr. 5b D-64807 Dieburg Fax: +49. 6071. 968-499

Coherent, Inc. Laser Products Div. 3210 Porter Dr. Palo Alto, CA 94304, U.S.A. Phone: +1. 415. 493-2111

Coulter Corporation P.O. Box 169015 Miami, FL 33116-9015, U.S.A Phone: + 1. 305. 380-3800 Fax: + 1. 305. 380-8312 E-mail: [email protected] Internet: http://www.coulter.com

Antibodies

Laser Manufactures, optical filters

Laser Manufactures, optical filters

Flow Cytometer systems: EPICS-maschines PROFILE optical filters

Appendices 333


Coulter Electronics GmbH Europark Fichtenhain B13 D-47807 Krefeld Phone: Fax:

+49. 2151. 333-5 +49. 2151. 333-633

Internet: http://www.coulter.com

Coulter -Immunotech Diagnostics Raboisen 5 D-20095 Hamburg Phone: +49.40.32-2180 Fax: +49. 40. 32-3969 Internet: http://www.coulter.com

Cyanotech Corporation 73-4460 Queen Kaahamann Hwy. Suite 102 Kailua-Kona, HI 96740, U.S.A. Phone: + 1. 808. 329-4677

1. 800. 453-1187 Fax: + 1. 808. 329-4533 E-mail: [email protected] Internet: http://www.cyanotech.com

Cytec Development 46560 Fremont Boulevard, Suite 116 Fremont, CA 94538, U.S.A. Phone: +1. 510. 657-0102 Fax: + 1. 510. 657-0151

Cytomation Bioinstruments GmbH Engesserstr. 4 D-79108 Freiburg Phone: +49.761.5597-370 Fax: +49. 761.5597-375 E-mail: [email protected] Internet: http://www.cytomation.com

Dianova GmbH Klein Fontenay 1 D-20354 Hamburg Phone: +49.40.4506-70 Fax: +49. 40. 4506-7390 E-mail: [email protected] Internet: http://www.dianova.de

Flow Cytometer systems: EPICS-mas chines PROFILE optical filters

Antibodies

Antibodies, Phycobiliprotein

Tissue Culture, Tubes and others

Flow Cytometer systems: MoFlo-maschines

Antibodies

Dianova-Immunotech Raboisen 5 D-20095 Hamburg Phone: +49.40.32-2180 Fax: +49. 40. 32-3969

Distrilab BV P.O. Box 130 NL-3830 AC Leusden, The Netherlands Phone: +31.33.494-7834 Fax: +31.33.494-8975

Dow Elanco Derquinter 92 B-2018 Antwerpen, Belgium Phone: 03.247-4225 Internet: http://www.elanco.com

Duke Scientific Corporation P.O. Box 5005 Palo Alto, CA 94303, U.S.A. Phone: +1. 415. 424-177 Fax: + 1. 415. 424-1158

Duke Scientific Corporation in Europe see Distrilab BV

DUNN Labortechnik GmbH Thelenberg 6 D-53567 Asbach Phone: +49. 2683. 43094 Fax: +49. 2683. 42776

Elanco Products Company see Eli Lilly Internet: http://www.elanco.com

Eli Lilly and Company see Dow Elanco

Eppendorf - Netheler - Hinz GmbH Barkhausenweg 1 D-22339 Hamburg Phone: +49.40.53801-0 Fax: +49. 40. 53801-593 E-mail: [email protected] Internet: http://www.eppendorf.com

Appendices 335

Antibodies

Fluorescent micro spheres

Antimitotic agents

Fluorescent micro spheres

Dyes and Antibodies

Labor utilities


EXCITON, Inc. P. O. Box 31126 Overlook Station, Dayton Ohio 45431, U.S.A. Phone: +1.513.252-2989 Fax: +1.513.258-3937

F. Hoffmann-La Roche AG CH-4070 Basel Phone: +41.61.6881111 Fax: +41. 61. 6919391 Internet: http://www.roche.com Switzerland

Falcon see Becton Dickinson

Faust Lustheide 58A D-51427 Bergisch Gladbach Phone: +49. 2204. 60362

Fisher Scientific Company Pittsburgh - Pennsylvania, U.S.A. Phone: +1. 412. 963-3300

Flow see Serva

Flow Cytometry Standards Corporation P. O. Box 12621 Research Triangle Park, NC 27709, U.S.A. Phone: +1.919.967-9345 Fax: +1.919.967-7325

Fluka Chemical Corporation 980 So. Second St. Ronkonkoma NY 11779-7238, U.S.A. Phone: +1.516.467-0980 Fax: +1.516.467-0663

Fluka Feinchemikalien GmbH see Sigma-Aldrich-Chemie GmbH

Fuji Photo Film USA, Inc. 400 Commerce Blvd. Carlstadt, NJ 07072, U.S.A. Attn. Consumer Information Services Phone: + 1. 800. 659-3554, Press 34 Internet: http://www.fujifilm.com

Antibodies

Biochemicals

Biochemicals

Chemicals

Calibration Particles


Photo equipment

Gibco BRL AG see Life Technologies GmbH

Gibco BRL Life Technologies INC. 3175 Staley Rd. Grand Island, NY 14072, U.S.A. Phone: + 1. 716. 773-0700 Fax: 1.800.331-2286

Gibco Life Technologies P.O. Box 6009 Gaithersburg, MD 20884-9980, U.S.A. Phone: + 1. 301. 840-8000 Fax: + 1. 301. 258-8238 Internet: http://www.lifetech.com

Glen Spectra Ltd. 2-4 Wigton Gardens Stanmore, Middlesex HA7 1BG, United Kingdom Phone: +44.181.2049517 Fax: E-mail: Internet:

+44. 181. 2045189 [email protected] http://www.isa-gs.demon.co.uk

Biochemicals

Biochemicals

Interference filter

~rr ~9 BDH Laboratory Supplies Broom Road Poole, BH12 4 NN, United Kingdom Phone: +44. 202. 745520 Fax: +44. 202. 745520

Hamamatsu Photonics Deutschland GmbH Photodiodes Arzbergerstr. 10 D-82211 Herrsching am Ammersee Phone: +49. 8152. 375-0 Fax: E-mail: Internet:

+49. 8152. 2658 [email protected] http://www.hpkco.jp/hpke.htm

Heidland Technische Gewebe Postfach 1921 D-33249 Giitersloh Phone: +49. 5241. 20094 Fax: +49. 5241. 26805

Gauze

Appendices 337


Heraeus Feinchemikalien u. Forschungsbedarf GmbH Heraeusstr. 12-14 D-63450 Hanau Phone: +49. 6181. 35-5215 Fax: +49. 6181. 35-612 E-mail: [email protected] Internet: http://www.heraeus.com

IBL Research Products INC. 67 Rogers St. Cambridge, MA 02142, U.S.A. Phone: 01. 617. 868-0077 Fax: 01. 617. 661-6341

ICN Biomedicals GmbH Thtiringer Str. 15 D-37269 Eschwege Phone: +49. 180. 2306160 Fax 0130. 868366 E-mail [email protected] Internet: http://www.icnpharm.com

Immunotech S.A. Luminy case 915 F-13009 Marseille cedex 9, France Phone: +33. 91758200 Fax: +33. 91412794

KMF - Laborchemie Handels GmbH Zum Siegblick 37 - 39 D-53757 St. Augustin-Buisdorf Phone: +49. 2241. 9685-0

0130. 852029 Fax: +49. 2241. 9685-35 E-mail: [email protected]

Labor maschines

Antibodies

Biochemicals

Antibodies

Antibodies

Kodak AG Photo equipment Gutenbergstr. 7 D-88046 Friedrichshafen Phone: +49. 7541. 51815 Fax: +49. 7541. 52218 Internet: http://www.kodak.com

Labokron (Kronlab) Postfach 1751 Dorntelsberg Sa D-74877 Sinsheim Phone: +49. 7261. 64993 Fax: +49. 7261. 3387

Tubes

Life Technologies GmbH Technologiepark Karlsruhe Emmy-Noether-Str. 10 D-76131 Karlsruhe Phone: +49. 721. 6189-0

0130. 8309-02 Fax: +49. 721. 6189-500

0130. 8334-35 E-mail: [email protected] Internet: http://www.lifetech.com

L.O.T. - Oriel GmbH 1m Tiefen See 58 D-64293 Darmstadt Phone: +49. 6151. 8806-0 Fax: +49. 6151. 84173

Merck, E. (see also Faust) Postfach 4119 Frankfurter Str. 250 D-46293 Darmstadt Phone: +49. 6151. 72-0 Fax: +49. 6151. 72-7521

Millipore GmbH Hauptstr. 87 D-65760 Eschborn Phone: +49. 6196. 494-161 Fax: +49. 6196. 43901

Biochemicals

Optical supply houses: Arc lamp systems and power supplies

Biochemicals

Filtrations systems

Internet: http://www.millispider.millipore.com

Miltenyi Biotec GmbH Friedrich Ebert -Str. 68 D-51429 Bergisch Gladbach Phone: +49. 2204. 83060 Fax: +49. 2204. 85197 Internet: http://www.miltenyibiotec.com

Miltenyi Biotec Inc. Suite 208 251 Auburn Ravine Road Auburn, CA 95603, U.S.A. Phone: 1. 800. 367-6227 Fax: +1.916.888-8925

Molecular Probes Inc. 4849 Pitchford Avenue Eugene, OR 97402-9165, U.S.A. Phone: +1.541.456-8300 Fax: + 1. 54l. 344-6504

MACS-Cell Separators and Reagents

MACS-Cell Separators and Reagents MiniMACS MidiMACS SuperMACS VarioMACS


Appendices 339


Newport Corporation 18235 Mt. Baldy Cir. P. O. Box 8020 Fountain Valley, CA 92728-8020, U.S.A. Phone: + 1. 714. 963-9811

Omega Optical INC. 3 Grove St. P. O. Box 573 Brattleboro, VT 05302, U.S.A. Phone: + 1. 802. 254-2690 Fax: +1.802.254-3946 Internet: http://www.omegaftlters.com

Omega Optical Inc. Distributor in Germany see PhotoMed

Omega Optical Inc. Distributor in UK see Glen Spectra Ltd.

Optical Instrument Laboratory Houston, Texas

Oriel GmbH see L.O.T. - Oriel GmbH

Ortho Diagnostic Systems GmbH Postfach 1320 Karl-Landsteiner Str. 1 D-69151 Neckargemiind Phone: +49.6223.77-216 Fax: +49. 6223. 77-278

Ortho-Mune Monoclonal Antibody Div. Ortho Diagnostic Systems Inc. Route 202 Raritan NJ 08869, U.S.A. Phone: +1.201.218-1300 Fax: +1.201.218-8582

Pall Deutschland GmbH Postfach 102120 Philipp-Reis-Str. 6 D-63303 Dreieich Phone: +49.6103.307-0 Fax: +49. 6103. 34037

Optical supply houses: optical tables, breadboards and mounts

Interference Filter

Optical instruments

Flow Cytometer systems: CYTORON

Dyes, Biochemicals and Antibodies

Filtrations Systems

Partee GmbH Otto-Hahn-Str. 32 D-48161 Munster Phone: +49. 2534. 8008-0 Fax: E-mail: Internet:

+49. 2534. 8008-90 [email protected] http://www.partec.de

Pharmacia Biotech GmbH Munzinger Str. 9 D-79111 Freiburg Phone: +49. 761. 4903-0 Fax: +49. 761. 4903-247

Pharmacia LKB Biotechnology AB 751 82 Uppsala, Sweden Phone: 46. 181. 63000 Fax: 46. 181. 43820

Pharmingen - Deutschland GmbH Haus A Flughafenstr. 54 D-22335 Hamburg Phone: +49. 40. 532-84480 Fax: +49. 40. 531-5892

PharMingen Attn: Customer Service Department 10975 Torreyana Road San Diego CA 92121-1111, U.S.A. Phone: +1.619.812-8800

1. 800. 848 - MABS(6227) Fax: + 1. 619. 812-8888 Internet: http://www.pharmingen.com

Phoenix Flow Systems 11575 Sorrento Valley Road Suite 208 San Diego, CA 92121, U.S.A. Phone: +1. 619. 453-5095 Fax: +1.619.259-527668

Flow Cytometer Systems: P AS-mas chines Cell Analyzer CA Biochemicals

Labor utilities

Biochemicals

Antibodies

Antibodies

Software products for the PC: MultiCycle-DNA analysis software MultiReport -Clinical Flow Cytometry Report generator software Multi2D-Bivariate data analysis software MultiTime-Kinetic vs Time data analysis software ListView-list mode analysis software Lab Reporter-Clinical Report Generator software ACQCYTE-Data acquisition software for flow cytometry

Appendices 341


PhotoMed Buro Sud Aalstr. 11 D-82266 Bachern a.W. Phone: +49.8143.94-303 Fax: +49. 8143. 94-304 Internet: http://www.omegafilters.com

Pierce Europe B.V. (see KMF) P. O. Box 1512 3260 BA oud Beijerland, The Netherlands Phone: +31. 1860. 19277 Fax: +31. 1860. 19179

Pierce P. O. Box 117 Rockford, IL 61105, U.S.A. Phone: + 1. 815. 968-0747 Fax: +1.815.968-8148

PolyLabo F-67000 Strasbourg

Polysciences, INC. 400 Valley Roadenzyme Warrington, PA 18976-2590, U.S.A. Phone: + 1. 215. 343-6484

1. 800. 523-2575 Fax: + 1. 215. 343-0214

Polyscience Europe GmbH Handelsstr. 3 D-69214 Eppelheim Phone: +49. 6221. 76-5767 Fax: +49. 6221. 76-4620

Photometries GmbH SolIner Str. 61 D-81479 Munchen Phone: +49. 89. 799-580 Fax: +49. 89. 799-715 E-mail: [email protected] Internet: http://www.photomet.com

Interference Filter

Antibodies

Antibodies

Gauze

Dyes, calibration particles and substrates

Dyes, calibration particles and substrates

CCD-Camera

Photometries Ltd. 3440 East Britannia Drive Tucson, Arizona 85706, U.S.A. Phone: + 1. 520. 889-9933 Fax: +1.520.573-1944 E-mail: [email protected] Internet: http://www.photomet.com

Promega Ltd: Delta House Enterprise Road Chilworth Research Centre Southampton,SOI 7NS, United Kingdom Phone: +44. 703. 760225 Fax: E-mail: Internet:

+44. 703. 7607014 [email protected] http://www.promega.com

Promega - Deutschland GmbH High-Tech-Park Schildkrotstr. 15 D-68199 Mannheim Phone: 0130. 9140-67 Fax: E-mail: Internet:

0130. 9140-68 [email protected] http://www.promega.com

Reichelt Chemietechnik GmbH & Co. Engelstra6e 18 D-69126 Heidelberg Phone: +49.6221.31250 Fax: +49. 6221. 301227

Roche Diagnostics GmbH Biochemica Sandhofer Str. 116 D-68305 Mannheim Phone: +49. 621. 759-8545

0800.759-4152

CCD-Camera

Enzyme and Biochemicals


Labor utilities


Fax: E-mail: Internet:

+49. 621. 759-8509 [email protected] http://www.boehringer-mannheim.com

Appendices 343


Roche Diagnostics Corporation Biochemical Products 9115 Hague Road P. O. Box 50414 Indiapolis, IN 46250-0414, U.S.A. Phone: +1. 317. 849-9350

1. 800. 428. 5433 Fax: + 1. 317. 576-4065

1. 800. 428. 2883 Internet: http://www.roche.com/diagnostics

SCHOTT Glaswerke Postfach 2480 D-55014 Mainz Phone: +49.6131. 66-0 Fax: +49. 6131. 662003 Internet: http://www.schott.de

Sefar AG Moosstr.2 Ch-8803 Riischlikon, Switzerland Phone: 01. 724-6511 Fax: 01. 724-1525

SERV A Feinbiochemica GmbH & Co. KG see Boehringer Ingelheim GmbH

SIGMA -Aldrich Chemie GmbH Diagnostik Griinwalder Weg 30 D-82041 Deisenhofen Phone: +49.89.6513-1251

0130. 5155 Fax: +49.89.6513-1161

0130.6490

Geschaftsbereich Fluka Phone: +49.89.6513-0

0130.2341 Fax: +49.89.6513-1165

0130. 847766 E-mail: [email protected]


Color Glass Filter

Gauze


Fine Chemicals

SIGMA CHEMICAL COMPANY P. O. Box 14508 St. Louis MO 63178-9916, U.S.A. Phone: +l. 314. 771-5750

l. 800. 521-8956 Fax: +l. 314. 771-5757

l. 800. 325-5052

Southern Biotechnology Associates INC. (SBA) P. O. Box 26221 Birmingham, AL 35226, U.S.A. Phone: + l. 205. 945-1774 Fax: + l. 205. 945-8768

Southern Biotechnology Associates INC. Germany see DUNN Labortechnik GmbH

Spectra-Physics 1269 W. Promona No. 112 Corona, CA 91720, U.S.A. Phone: + l. 909. 278-0563 Fax: E-mail: Internet:

+ l. 909. 278-4887 [email protected] http://www.spectraphysics.com

Spectra-Physics GmbH Siemensstr. 20 D-64289 Darmstadt Phone: +49. 6151. 708256 Fax: +49. 6151. 710795 Internet: http://www.spectraphysics.com

Spindler & Hoyer GmbH & Co. Postfach 3353 Konigsallee 23 D-37081 Gottingen Phone: +49. 55l. 6935-0 Fax: +49. 551. 6935-166 E-mail: [email protected] Internet: http://www.spindlerhoyer.de

Fine Chemicals

Dyes and Antibodies

Laser Manufacturers: Argon and Krypton laser CW dye lasers He-Ne laser

Laser Manufacturers: Argon and Krypton laser CW dye lasers He-Ne laser

Optical supply houses: mini-bench series of small components and mounts Laser Manufacturers: Yellow,orange,red He-N e laser

Appendices 345


SPSS Federal Systems (U.S.) Courthouse Place 2000 North 14th, Suite 320 Arlington, VA 22201, U.S.A. Phone: +1. 703. 527-6777 Fax: +1.703.527-6866 E-mail: [email protected]

VERITY Software House, Inc. P. O. Box 247 45A Augusta Road Topsham - Maine, 04086, U.S.A. Phone: + 1. 207. 729-6767 Fax: +1.207.729-5443 E-mail: [email protected] Internet: http://www.vsh.com

Whatman International Ltd. Whatman House St. Leonard's Road 20120 Maidstone Kent, ME16 OLS, United Kingdom Phone: +44. 1622.626262 Fax: +44. 1622.677011 Internet: http://www.whatmann.com

Wild Leitz-Vertrieb Deutschland GmbH Mainzer Landstr. 625 D-65933 Frankfurt Fax: +49. 69. 39002-188

World Precision Instruments International Trade Center 175 Sarasota Center Boulevard Sarasota, Fl34240-9258, U.S.A. Phone: + 1. 941. 371-1003 Fax: +1. 941. 377-5428 E-mail: [email protected]

W ourld Precision Instruments Liegnitzer Str. 15 D-10999 Berlin Phone: +49.30.6188-845 Fax: +49.30.6188-670 E-mail: [email protected] Internet: http://wpi.sireco.de

Sigma Plot spreadsheet and graphics programs for the PC

Software products for the PC and Macintosh: IsoContour, 2-parameter Isometric, Contour Generator, ModFit, DNA Histogram Modeling Program, ReticFit

Optical filter paper

Microscopes

Labor utilities

Labor utilities

Yakult Honsha Co. Ltd. 1-1-19 Higashi Shinbashi Minato-ku Tokyo, 105, Japan

Zeiss, Carl ZW. Ndrl. Miinchen Perchtinger Str. 8 D-81379 Miinchen Phone: +49. 89. 74840-0 Fax: +49. 89. 74840-149 Internet: http://www.zeiss.de

Zynaxis Cell Science INC. 371 Phoenixville Pike Malvern, PA 19355, U.S.A. Phone: +1.215.889-2200 Fax: +1.215.889-2222

Cytometry on the Internet

Interesting Links on the Internet:

Cytometry on the Internet


Optical glases

Chemicals

- http://www.biochem.mpg.de/research-groups/valet/ cytorel.html

Flow Cytometry on the Weg - http://carmen.salk.edu/fcm/sites.html

Compendium of Cytometry Internet Sites - http://nucleus.immunol.washington.edul/ISAC.html

Flow Cytometry Laboratory - http://www.genetik.uni-koeln.de/flow_cytometry/ (more interesting Links for topics related to "Cytometry") - http://www.cyto.purdue.edu - http://ubedu.uibe.ch/insel! cycle.html

Flow Cytometry Consortium WebPage - http://www.cber.nih.gov/wekome.html

Magnetic Cell sorting - http://www.miltenyibiotec.com

Appendices 347

Subject Index

A 7-AAD 117 -118 Acid elution 207 acidic pH optimum 179 acidification kinetics 187 acquisition software 19 adhesion molecules (homophilic inter-

action) 87 affinity of a ligand 82 aggregates in the sample 22 alfa-bromonaphthalene 281 alkalinization of intracellular pH 159 amiloride-sensitive 168 amiprophos-methyl 281 amplification 296 amplitude 249 analog signal pulse - digital signal 10 Annexin V -MicroBeads 64 anti BrdU staining 109 Anti IgG2a(a) 106 Anti-BrdU antibodies 106 anti-cytokine antibodies 66 Anti-IgG1(a) 106 antibody-conjugates 15 antibody-secreting hybridoma cells 71 APC fluorescence 9 aphidicolin 281 argon laser 132 arithmetic mean 48 aspartic proteinases inhibitable 177 autofluorescence from chlorophyll 309 auto fluorescent 305

B B-cells 204 band-pass (BP) 308

bandpass filter 167 bandpass- (BP) - bandfilters 23 p-Galactosidase 183 bicyclic GSH protein adducts 176 biochemical processes 159 bioclean microbeads 88 biotin-streptavidin 40 biotinylated antibody 52 Biotinylation of the antibody 29 blocking of the free binding sites 39 blood dendritic cells 238 BrdU-Hoechst 118 BrdU-Hoechst quenching 112 BrdU-staining 64 Bromodesoxyuridine (BrdU) 105

C Ca2+ efflux 135 Ca2+ influx 135 calcium-sensitive dye indo-1 140, 144 CALEB beads 94 Calibration 45 calibration of compensation 10 calibration routines 4 Calibration standards 13 Capping 42 carboxy-seminaphthordhodafluor-1

(SNARF-l) 159 catching-antibody 71 cathepsin G 177 cell clumping 107 cell cycle analysis 10 cell debris 12 cell surface affinity matrix 71 cell's physiology 4 cellular control 39

350 Subject Index

central wavelength 24 chemoattractants 86 chemorepellants 86 Chinese hamster ovary (CHO) cells 214 Chlorix 321 Chromomycin A3 268, 282 chromomycinlHoechst 33342 290 Chromosome sorting 266 class-specific inhibitors 177 Clinical-grade magnetic cell sorting 228 clogging of the nozzle 262 co-transfected 2ll coaxial laminar stream 294 coefficient of variation 46 colchicine 281, 290 compensation 45 Complement Lysis 205 complement-mediated lysis 197 conjugated 186 Contamination 233 contour plots 46 Cotton Wool Columns 198 Coulter Elite 295 count mode 262 covalently (covaspheres) 88 cross linking 2ll cross-reactions 40 cross-reactive antibodies 52 CV 46 Cy5 186 Cychrome stained cells 17 cylindrical circumference 296 cysteine proteinases inhibitable 177 cytokine 2 66 cytokines 66 Cytophilic Antibodies and Antibodies

206 cytosolic free Ca2+ concentration 164 cytosolic free calcium concentration 159 cytotoxic T lymphocyte line (CTLL) 113

D DAPI 281 DCH 169 dead cells 46, 201 dead-time control 307 debris 46, 308 deflection plates 250 Dendritic cells 58

density of target antigen 48 depletion 222 depletion of erythrocytes 39 de stain 206 Determine the coupling ratio 32 4',6-diamidino-2-phenylindole (DAP!)

lOl dichroic 305 dichroic filter 144 Dichroic mirrors (DM) 308 Dichroic mirros (DC) 302 Dicroic beamsplitters 24 2,3-dicyanohydroquinone (DCH) 159 different molecules (heterophilic inter-

action) 87 dig-anti-dig 40 Dig-labelled antibody 80 digoxigenin (dig) 40 dihydrorhodamine 123 168 diisopropylphosphofluoridate (DFP)

177 dim fluorescence 9 Direct staining 39 discrimination of rare cells 259 disturbance 296 DMSO 131,281 DNA amplification 278 DNA dyes 57 DNA polymerase activity 169 DNA-specific fluorochromes ll3 dot - plots 16 drop drive 249 drop drive amplitude (DDA) 3ll drop drive frequency 249 drop drive frequency (DDF) 3ll droplet formation 294 dual-laser analysis 177 dye laser 18 Dynabeads 42

E E-64 177 EB ll8 efflux 135 EGTA 131 electro neutral Na+lH+ antiport 168 electronic noise 12 electronic signal detection 9

electroporation 212 emission wavelength of fluorochromes 7 endocytic compartments 186 endocytosis 186 enrich mode 262 enzymatic activities 4 EPICS 302 epitope of the secreted molecule 74 Erythrocytes 200 ethidium bromide 282 ethidium bromide (EB) 113 excitation wavelength of fluorochromes

7 expression of cytokines 59 expression of secreted products 71 expression of two parameters per cell 47

F FACStar 307 FACStar system 307 Fc receptors 207 ferromagnetic matrix 220 ferromagnetic structures 220 Ficoll gradients 197 fIxation 43 fIxation of stained cells 38 fIxed cells 15 fIxed-time flow cytometry 141 FLl-O/OFL2 compensation 17 FL2-0/0FLl compensation 17 FL2-0/0FL3 compensation 17 FL3-0/0FL2 compensation 17 flow cuvette 4 flow stream 296 flow tip 298, 303 flow-in-air systems 4, 32l fluidics control 14 fluo-3 128, 159, 166 Fluorescein-p-di-galactoside (FDG) 183 fluorescence emission of PerCP, CY7 9 Fluorescence Intensity (IF) 284 Fluorescence Intensity (PF) 284 fluorescence multicolor compensation

60 fluorescence quenching 91 fluorogenic indicators 159 FLUOS 30 fMLP 169 formaldehyde-fIxed lymphocytes 15

Subject Index 351

Forward scatter 7 Forward-Angle Light Scatter (FALS) 284 forward-angle scatter (FSC) 308 fura-2 128

G Go/G j + S + GzM 118 Go/G j cells 112 Gj 118 GO/G 1 phase 278 G2/M phase cells 10 gain (G) 9 yGlu-CysH-Gly 175 gating 45 geometric mean 48 glutaraldehyde fIxed chicken erythrocytes

(CRBC) 20 glycoproteins 86 granulocytes 201 GSH 175 GSH S-transferase 176 GSH S-transferase isozymes 176 GSH-OPT adducts 176 GSH-S transferase 175

H hapten 73 hapten-conjugated 234 haptens 29 HeCd laser l32 Helium/Neon laser 18 hematopoietic stem cells 77 heterogeneous population 141 heterophilic aggregates 94 high frequency 251 high gradient magnetic cell sorting

(MACS) 211 high gradient magnetic fIelds 218 high numerical aperture 7 high-gradient magnetic cell sorting 228 high-gradient magnetic field column 78 histogram plots 16 HL60 113 HLA-DR 58 Hoechst 282 Hoechst 33258 268 Hoechst 33342 284 Hoechst, FITC, PE 9 human blood dendritic cells 238

352 Subject Index

human hematopoietic stem 228 Hydrodynamic focusing 294 hydrodynamic focussing 4 hydrodynamically focussed 19 hydroxyethylpiperazine ethanesulfonic

acid (HEPES) 161 hydroxyurea 281 hypotonic shock 200

I IFN -y 66, 68 IgGI secreting cells 74 IL-4 68 IL-5 68 ILIa and ~ 66 Illumination optics 6 illumination point 324 immunofluorescence 53 Incidin 321 indo-l 128, 159 indo-l/Ca2+ complexes 166 Influx of Ca2+ 127 Internalization kinetics 191 intra- and extracellular Ca2+ influx 129 intracellular Ca2+ 127, 135 intracellular cytokines 58 Intracellular GSH levels 175 intracellular IL-2 68 intracellular immunofluorescence 40 Intracellular pH 168 intracellular staining 58 intracellular staining of fIxed, dead cells

71 intracytoplasmic 201 ion fluxes 4 ionomycin 142, 167 iron-dextran particles 220 isolated metaphase chromosomes 266 isotype control 39

J jet-in-air systems 294 Jurkat 113

K K+ concentration 169 kinetic analysis program MTIME 133

L L-Leucine methoyl ester 203 lacZ 183 laser beam 298 laser focus 10 laser intersection point 20 laser safety 20 latex beads 13 LDS 730 54 LDS 751 43,54, 118 - uv -capable ion argon lasers 117 LDS 798 54 LDS-751 64 LDS730 57 LDS751 57 LDS798 57 ligand 186 ligand acidifIcation 186 ligand binding 82 Ligand conjugates 82 linear and logarithmic amplifIers 12 lipofection 212 liposomes 42 lissamine rhodamine sulfonyl chloride

(LRSC) 82, 186 live gate 12 live gating 45 logarithmic amplifIcation 45 logarithmic scale 12 long-pass (LP) 308 longpass (LP) fIlters 302 longpass fIlter 167 longpass- (LP) - bandfIlters 23 low numerical aperture 7 Lysis 200

M macrophages 173 MACRO sort 307 MACS 74,218 magnetic beads 73 magnetic cell sorting 256 Magnetic depletion 236 magnetic labeling 232 magnetic MicroBeads 211 magnetic particles 218 Magnetofluorescent liposomes 77 mapping genes 273

maximum transmission points 24 mean fluorescence 188 mercury orange 177 metalloproteinases inhibitable 177 Microbeads 54, 87 mithramycin 281 Modfit 51 molar ratio of fluorescein to protein 32 molar ratio of tetramethylrhodamine to

protein 32 MOLT3 113 monoclonal antibodies 4, 78 monoclonal antibodies (mAbs) 129 monoclonal antibodies to BrdU 105 monocytes 173, 203 MPCll plasmacytoma 113 Multicycle 51 multisort bead-release technology 242 multisort microbe ads 224

N (NADPH) oxidase 172 90° -angle side scatter 308 neutrophils 173 Ng-CAM coated beads 94 nigericin 142, 169 NIH3T3 113 nitro-iodophenylacetyl (NP) 40 Non specific-binding 33 non-magnetic fraction pre-enriched 240 non-mitotic cells 279 nonseparate populations 51 nonspecific fluorescence 84 nonsymmetrical histograms 51 nozzle position 18 nozzles 4 NP-anti-NP 40 N rCAM beads 94

o optical fllters 13, 23 oryzalin 281 overlapping populations 51

P P19 cells 141, 152 panning 197,204 paraformaldehyde 108 PAS 321

PCR 278 PCR amplification 267 Peak 284 pepstatin 177 PerCP 54,60 peripheral blood 55 peripheral blood mononuclear cells 201 permanent cell lines 113 permeabilize cells 64 PH calibration 189 pH sensitive fluorescent probe 186 pH-insensitive 190 pH-regulatory mechanism 168 pH-sensitive 190 phagocytic cells 173-174 phase 249 phenanthroline 177 Phoenix Flow Systems 116 phosphoric amide herbicides 281 Photobleaching 61 photomultiplier tubes (PMT) 9 phycobiliproteins 29 - 30, 54, 186 physiological fluid 12 piezoelectric crystal 296, 311 piezoelectric transducer 249, 293 plasma membrane potential 159 pluronic F-127 168 PMT voltage 9 polyamine buffer 268 population of lymphocytes 16 positive selection 222 Pre-enrichment 198 pre-enrichment methods 57 Preenrichment 38 preselected electronically (gated) 45 preselection 256 pressure-sensitive 149 pressure-sensitive cells 141 primary blocking antibodies 80 progenitor cells 228 proliferating cell nuclear antigen

(PCNA) 112 proliferative history of a cell 4 propidium iodide (PI) 113 protein-producing cells 63 Pulse area 10 Pulse processing 10 pulse width 10

Subject Index 353

354 Subject Index

Q quantify DNA content 4 quantitative fluorescence measurements

54 quenching effect 122 quin-2 128

R R-phycoerythrin 174 R-phycoerythrin-Cy5 tandem 174, 179 rare cells 38, 43 rare event analysis 58 ratio 188 receptors for cytokines 77 recovery of rare cells 261 Reduced Glutathione 175 rejection bandfilters (RB) 23 rhodamine 110 (R110) derivatives 160 rhodamine 123 172 rhodamine conjugates 53

S Sand G2M 118 S phase 169 S- and M-phase 289 SA -CyChrome 66 SaDig-fluorescein 80 SaDig-liposomes 80 sample fluid 4 sample stream diameter 82 sample tube 4 Satellite droplets 314 Scatchard analysis 83 second fluorochrome 29 secreted products 71 sedimentation 263 self-quenching 59 semiconductor photodiode is 9 sensitivity of fluorescence 4 sequential points 294 serine proteinases inhibitable 177 sheath fluid 12 Sheath pressure 5, 18, 298, 303 sheep anti-digoxigenin Fab-fragments

78 shifted populations 51 short- (SP) - pass ftlters 302 short-pass (SP) 308

shortpass- (SP) - bandftlters 23 single cell 38 small fluorochromes 29 SNARF-l 169 sodium hypo chloride 321 sorting of rare cells 198 specific binding 84 specific Na+/H+ antiport inhibitor

amiloride 172 specifity of the fluorescent conjugates 83 spectra of the dye 9 spectral overlap 303 Staining of cytoplasmic 42 Standard fixation 44 sterile sorting 258 stimulated cells 140 stream-in-air flow 83 stroboscopic illumination. 294 super-paramagnetic particles 218 surface markers 210 surface receptors 84 surface-immunophenotype 63

T T-cells 204 T-junction 145 tandem conjugates of Cy7 9 Texas red 30 Texas red conjugates 53 Texas red to protein 32 three color - RED670 15 Three-dimensional plots 48 threshold gating 51 thrombocytes 201 thymidine 105 time as a parameter 188 titration 42 transfectants 143 transferred - cells 210 transferrin (Tf) 82, 186 transient transfection 210 trigger 45 trigger level 16 trigger threshold 12 Triggering on DNA fluorescence 12 tripeptide glutathione 175

tumor cell lines 112 Tumor necrosis factor-a 169 tumor promoter phorbol12-myristate 13-

acetate (PMA) 173 Tween 20 101,282 TX - fluorescence 9

u unconjugated catch-antibody 74 unconjugated catching-antibody 76 unstained polystyrene beads 13 UV excitation 89 UV laser 18

V variation 128 variation of measurement 48 voltage (V) 9 volume flow rates 83

Subject Index 355

appendices - springer978-3-662-04129-1/1.pdf · also used for pumping dye lasers with 514 nm light....

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