applicability of qpcr to ensure the quality of milk and ... · applicability of qpcr to ensure the...
TRANSCRIPT
Applicability of qPCR to ensure the qualityof milk and dairy products
Bibliography(1) Fusco V., et al., 2010 International Journal of Food Microbiology (144)(2) Ducauze C.J., 2003 Fraudes alimentaires, Approcheréglementaire et méthodologie analytique(3) Di Pinto A., et al., 2006 Food Control (17)(4) Riyaz-Ul-Hassan S., et al., 2009 The Journal of dairyresearch (762)(5) Lees M., 2003 Food authenticity and traceability(6) Scarpellini M., et al., 2004 FEMS Microbiology Letters (236)(7) M. Jodra M., Li E., 1993 International Journal of Food Microbiology (18)
IntroductionIn food processing industry,
quality is an unavoidableelement, so much for consumers’health as for organoleptic qualityof the products. Milk processingis highly involved because milk issubjected to variouscontamination sources, beforemilking (soil, food, water orexcrement), during milking(milking people) and after milking(storage, transport or processing).As qPCR is booming, wewondered if it was applicable toensure the quality of milk anddairy products.
ConclusionIt has been proven that qPCR is areliable method, that can be usedon various stages of milkprocessing (just after milking,before and after transport, afterindustrial processing…). qPCR canalso be used in other foodprocessing, nevertheless liquidsare easier to analyze than solidsdue to representativeness of thesample (meat, eggs…).
Organism: StaphylococcusaureusSource: milking people (dirtyhands)Effect: food poisoningDetection kit: mericon S.aureus KitTargeted gene: Egc(enterotoxin gene cluster)1
Organism: MycobacteriumtuberculosisSource: infected cowEffect: tuberculosisDetection kit: QuantiFastProbe PCR KitsTargeted gene: mpb70 gene3
Organism: Escherichia coliSource: cow defecationsEffect: food poisoningDetection kit : mericon PCRDetection kitsTargeted gene: gene uidA 4
Organism: GMOSource: food (silage, cornand soy)Effect: ?Detection kit: mericon GMOdetection assaysTargeted gene: severalsequences are specific toGMO, like the couplesCry03/04, PEP-C2/Btk1,P35S-f2/pétu-r1, IVS2-2/PatB. 2
Organism: PseudomonasfluorescensSource: water, soil…population increases whenstorage is too long at 5°CEffect: spoiling (lipolysis,proteolysis)Detection kit: QuantiFastProbe PCR KitsTargeted gene: gene ITS16
Organism: AdulterationSource: deliberate, duringindustrial processing e.g.cow’s milk in other milks(goat, ewe, buffalo…)Effect: allergy, lack of qualityDetection kit: QuantiFastProbe PCR KitsTargeted gene: cytochrome-b gene (specific to cows) todetect cow’s in goat’s milk.5
Organism: Aspergillus flavusSource: bottling, food?Effect: secretion of AflatoxinB1 (carcinogen)Detection kit: QuantiFastProbe PCR KitsTargeted gene: multicopyITS2 rDNA 7
C. LEMAITRE, E. LABBE, T. RIBEIRO, A. CHANGO, A. M. ABDEL NOURInstitut Polytechnique LaSalle BeauvaisSpécialité Alimentation Santé, promo 010,
19 rue Pierre Waguet, 60026 Beauvais, FranceE-mail: [email protected]
Equipment needed methods common to all organisms as we detect them in milk. For body fluids : QIAamp DNA Blood Mini Kit Rotor-gene Q (Qiagen)
qPCR instrument