/-;-.•.---------------~~=~.-_._----

N,O R T H M A H ~ R ASH T R A U N I V E R SIT Y.J A L GAO N _ 425 001.

..Ja~Lu..BUS2.Q!L!'Ij..!2S_~.2..~~(P~T__:g:~(From June,1993)

J

"

11. BOT

12. DOT13. BOT

Plant Biotechnology.

3.2 Gymnosperms and Palaeobotany.3.31 Algae special paper _ I

I

BOT 3.32 Mycology and Plant pathologySpecial Paper_I

BOT 3.33 Ang{osperms special paper- IBOT 3.34 Plant Physiology special paper-I

14. BOT

15. BOT

SEMESTER IV

16. BOT17. DOT

Practical on BOT 3.1 and BOT 3.2

Practica! on special paper I(BOT 3.31 DOT 3.34)

4.1 Developmental Botany.4.21 Algae special paper II

,

BOT 4.22 Mycology and Plant pathology special paper-II

BOT 4;23 Angiosperms special Paper-II-'- BOT

18. DOT4.24 Plant Physiology special Paper-II.

4.31 Algae special Paper-III.

BOT 4.32 Mycology and Plant pathology specialPaper-Ill:

DOT 4.33 Angiosperms speciul paper III.,BOT 4,34 Plant Physiology Special Paper-III.

19. BOT

20. DOT

4.4 Practical on DOT 4.1.

Practicul on special papers II and III

{BOT 4.21-4.25 and DOT 4.3l-4.35},,

,,NOTE : All Course are University Courses.

for all University Courses will beExamination.

The examinationUniversity:

-,

•

"

,

M.Sc. Part_II

••

BOTANY (From JUne.1993)

SEMESTERIn DOT : 3d..£!.~&~chnology (48 Lect.ure),1. ~damental~__~~~otechn~~: Definition,Introduction.

Import~nt milestones and application. j

2. Fe:r:.n:!.e_nta.-!'~ig..~.ec~E9.!..99.Y..:anarobic fermentations,

.il Defination.' ii) Aerobic and

iii) Design of fermentors. iv) Paramenters of fermentations,v) Schematic representation of fermentation process,

primary and secondary metabolites, Alcohol, Organic acids,Vitamins and Antibiotics. vi} Biochemical Engineeri,ng.

3. ~m",s5 technol0.9Y...f':'FJood•. feeE._und£ertiJ •.i_Z~.E.~..•:Single cell proteins its: need and applications, Massculture for proteins, mushroom cultivation, Cultivationof algae on waste as feed. Eiofertilizers types, massproduction and its advaotag~s over chemical fertilizers •

•4. Enzyme technology: (a) Technology of Enzyme production'(sources, media, extraction aod pUrification), its industrialapplications. (b) Immobilized enzymes and whOle cells idefinition, methods of immobilization and their uses in watertreatment, uses in synthesis of medical products.

5. ~_l.?gical Fue:.!.techl'l'?l-2.ill'_:Biomass as potential energysource, Energy plantation, utilizatio~ of Biomass forethanol and methane. Animal and industrial organic wastesas potential source for Fuel, Biological Fuel cell.

6) Genet~~~in~ering: Definition cutting o~ DNA joining ofDNA,restriction endonucleases, laggation, transformation,amplification, application in agriculture & medicine.

7. ll~0l2.'?1~n Agri,.,'2?-lture.£!l<!J'2res,t!1:: Plant cellculture, principles and ~pplications, clonal propagationof economically important plants, production of plants of.various ploidy levels and fusion, somatic nybridization,Suspension culture for production of piant metabilitessecondary plQnt products.

1. htkinson2. Baker, E.W. and

Venkataraman C"V.'3. Bhojwani, 5.5. and

Razdan, M.1<:.

Cultivation of edible mushrooms.: h menuQl on the cultivation andproceeding of algac as a source Of' Scpo

: Dev810pment in crop science (5)plant tissve culture theory and.practice.

4. Biotechnology Volumes.o:>nt •• 2

---

ccont •• 4

Contribut~ons of Indian Mycologists. InternntionalMycologic~l Institute (IMI). its services & Publications.

III) Modern trends in taxonomy of fungi; Ultrastructure,cell-wall composition, Sevta. Concept of hyphae,Mycelium, rhizomorphs and fruictifications.

IV) 11comprehensive OlCCOuntof structure, development.

reproduction, life cycles, classification. Phylogenyinterrelationshirs and evolutionary aspects of!'lyxomycota. Mastigornycotina. Zygomyc"tina.

Ascomycotina. Basidiomycotina. Deteromycotina and Lichens.-X-X-X-x-x-x_

BOT I 3.33

Angiosperms Speaial Paper_I (60 Lectures)r) Study of Cronquist's system of classification with respe.t

toaj Salient Features of the system.oj Characterization ~nd critical tendencies of subclasses.c) Discussion cn or,derS, Merits ~nd demerits of the system.

II) Mod~rn trends in taxanomy ,1. Ultrastructure. .

2. Serology.3. Wood anatomy.4. Cytochemistry.s. Diosynthetic pathway.6. Numeric<l.ltuxonomy.

III) Biosystematics_

Concept. Aims and objectives of biosystematics cat€gories andmethods in biosystematics. Comparison of classical t~xo-namy and Bicsystamatics.,-

IV) Floristics :Principles distinction between Flora and Vegetation. Floralcomposition and elements in the flora of Maharashtra andIndia.

..1l.c.!!._:-1_~";L4__PE..nt Physiology Spe~l Paper .I(48 Lectures)

.ICell_organiz?J:..i.£!!...:~ thods of studying cells structure,(gross & Ultra). functions of membrane.cell wall. plasmodesmata. plasma membrane,Mitrochondria. plastids, endosplasmic reti-culum. Lysosomes ribosomes.origin and

cont ••5

-~~----------------~-~--( .

cont •• S

evolution of Eukaryotic CGlls.

II). Genome. orgonisation. 5t~ucture of DN!>, RNA, DNh sequencing,RepliC<ltion of DNA,Transcription, RNAtype, RW,proce_ssing. genetics cQde. protein synthesis.

In) Chemistry of organic acids,phenols, alkaloids, tannins, 1igoios.pigments, terpanes, fl~vonoids. plont

•growth substances.

tv)B~~!-r£c~iCs : Definition, Bioenergetics ofphotosynthesis. Lumonescence. chlorophyllflourcscence.

V) ~tE ..~:rv:,nt~~i"!:1) Phase contrast microscope.2) Chromatogr<lphy (Different types)3) Electrophoresis.4) Colorimetry.5) PH. meter.

6) Spectrophotometer.C"; 7) Conductivity Bridge.

-x-x- x-x-x -x-X-X-'(- x-

cont, .6

.'

"""

,~..

-'"" ~

cont ••6

SEMESTER IV

80T t 4.1 Developmen~l BotAny (60 Lecturea)

h. lUlatomy I (24 Lectures)

1. Organization of plant body,2. Apical meristems & differentiation.3. Leaf formation and expansion stomata _ development and

classification.4. Vascular cambium.5. Differentiation of Xylem elements and their phylogenetic

specialization.6. Development of sieve tube elements and their ultra

structure.7. Structure of dicot & Gymnosperm Wood.8. Reaction wood, cause of reaction wood, formation.9. Development of stems in arborescent monocotyledons.

10. Floral Anatomy - Transition to flowering and developmentof lateral organs.

11. Structure of fruit and seed.

B. Embryology (24 Lectures)

B.l Microsporanoium - Development, structure and function ofmicro sporangium, micro sporogenesis, male gametophyte.

B.2 Meg~r~n~um - Development, structure and function ofmegasporangium, megasporogenesis, female gametophytedifferent types, nutrition of embryosac.

8.3 endOSperm I Development structure and function.B.4 ~_~ogenes:lO!. - Development of embryo (Monocot ahd dicot)

nutri lion embryo, structure of mature embryo Apomixis.8.5 Experimental <'Indapplied embryology pollen, Auther, pollen,

OVule & embryo culture.

C. Palynology (12 Lectures)

C.l Introduct!££ - concept, historical account, scope.C.2 Pollens I Development of pollen grains _ Meiotic and

Post-meiotic proces5~e,differentiation of wall layersExine str~tification, polarity, symmetry, NPC, pollenUltrastructure.

C.3 Spore/Pollen development in plants _ Algae to AngiospermsWE)ll comoosit.ionw.r.t. structure,lo~n~mentatron. aperture wall composition,

Algae to Angiosperms.

C.4 Applied palynology I - Pharmacopalynology Geopalyology,~erobiology, Pollen nllergy, Palynotaxonomy.

cant ••7

~,nolj

r

.• 4_~T I 4.21

cQnt •• 7

1I19aeSpecial Paper II

HYDROBIOLOGY II

1. Introduction, Review of Hydrobiological work in India., ., (~~2.Lentic enxironment- I General considerations, Physioo_

.e',,','{:t '\',-::;;-' T chemici'llfactors and their influencQ. Phytoplankton _~ ,20, quantity and qualitity, periodicity & succession,t o-::tlJp.t1 ,n vertical distribution, ad'lptationsof p1anktons. Biolo_ID <:Q,,?j) LnG gica1 prOductivity of Inland waters & factors influencing.I,tinE ','mo,cfiflon it.

1101jlaoqm(.3. Lotic environment I G€nerQ1 considerations, Physioco_:IO nol:t£,~

chemic''Ilfactors. Phytoplankton, quality. quantity,periodicity & succession, vartical distribution.e •• :t[lo," _Productivity & factors influencing on it.

4~ Marine environment I General considerations, Physico_chemic~l factors and their influence. Marine phytoplankton_Density, Diversity, Periodicity & succession, Verticaldistribution, Productivity & factors influencing on it.5. Eutrophication I

•

Indices.,

for eutrophication •

I Ecological classification of algae IBenthos 3~ Halophytes5) Cryophytes 6) Soil algae8) Epiphytes & endophytes,

10) Parasitic algae.

". '

1. Definition, factors responsible2. Biotic relationships.3. Effects of eutrophication on water blooms. '

.~~~4. Contr~l measures of cutrophicQtion & water bloom.5. Palmer's Index of Pollution and Nygaard's Tropic

Ecology of Algae1l Planktons 2)

" 4) Thermophytesn~"j'tOo. ""1-:.

~_~nal;,~7) Lithophytes.9) Symbiotic algae.

•

czonathion patt~rn, lifemarine algae of east &

7••~Marine benthic Algae ~General principles, Shore types,forms, geographic~l distribution,west coast of India•

.8. Sewage disp'?.~ Ifi it' composition of sewage,1. Introduction, de n lon,

necessity of sewage disposal.i 1 & biologicalNature of sewage _ Physical, chernca,2.

considerations of s~wagc treatment. '

, 3. Disposal of solids processimhoff tClnks.

4. hctivated sludge process &

& digestion, septic &

digestion.• cont••8

,,•

-cont •• S

5. Algal sttibili~ation ponds, flor~, succesion & periodic! tyof algae in sewage.

6. B2omonitoring of water quality.

BOT I 4.22 l1Y.Cology&J'..!.cm~ P@ology

SPECI1\L_Rl\..R.Jt~ _I_~

(48 Lactures)

Industrial Mycology I

Presercations and main~nce of cultures, methods ofsterilization, continuous, synchronous and phasecultures,Aerobic and anaerobic Fermentations, Parartleters of fennentors,Primary metabolites, Alcohol & AlCOholic beveranges, organicacids, secondary metabOlites-antibiotics, Qrowth regulators,vatamins, enzymes, AssaY-Physical chemical & blologlc~.

CuItrivation of edible fungi & S.C.P., ferrntnt<J.tion pf Tea,Coffee, Cocoa & rottingof fibres.

Fungal genetics_,

Incompatibility systems, 'Tetrad analYSis, Parasexualcycle, genetics ot industrial organisms.

I. Soil Microbiology ,

Structure and types of soirs, Biological population,Predation, parasitism, antagonism, mycorrhizal, associa_tion Rhyzoplane. Rhizosphera, Non-rhizosphere & Phylloplane,Decomposition of organic matt~r and nitrogen Fixation.

H.

tu.

IV. Funqal eCOlogy I

Heterotrophy and its consequence, Distribution &Dynamics, of fungi in enVironment. Practical exploita.tionOf saprotrophy.

-x-x-x-x-x_x_x_x_

EOT. I 4.23---Angiosea.!!!!. Spe~-i?-LpaPQr-:..XI (48 Lectures)

1. WoodAnato..'!!YI Vasoular Cambium,Hard und Soft WOods,

elelllents of Wood, their structure and distribution, figure

in WOod.grain and texture, Of wood, properties and us.es ofWOodin reltl.tion to structure and composition, Anatomyanaiaentificstion of important timbers, Chemical COmpositionof secondary plant cell Walls, methods Of Preparation ofWOodfor micrOSCOPicobservation.

ConT. __ Q

cont ••9

2.

IfIE~_ryO~0JaZ I Artifici~l Pollination, Fertilization, Sexual

incompatibility, Endosperm. ultrustructure ~nd histochemi_stry, Polyembryony, Embryo as reaction syst~m.HomOlogies,Experomentcl embryogenesis, different schools of Embryologyand thGir contributions. .,11

3. Palynology I Pollen units, pollen biochemistry and physiologyiIPollenkitt, Pollen Wall proteins, Pollen germination in Viva"

and in Vitro, Pollen culture, Pollen storage Dnd viability,Pollen sterility, Pollen morphism, Palynology aS~basis of angio~_,

sperm, Phylogeny, methods of Pollen preparation, Melito_palynology. Jt

4. ~hology I Theories concerning the naturc"of carpel andsternan in angiospems. H

NOTE I Special Paper _ II is of 48 i.e. 4+1 periods are,.

required (4 lectures + 1 tutorial each lecture is of60 minutes) • "~

"I,"BOT. 4~24 Pl~nt P~ysiology Speci~aper_II

,

(60 Lectures) il

c

1. Water relation of plants : Leaching of substances fromplants, Exudates, stomatal physiOlogy, transfer and tr~nsport

"systems in plants, salt stress physiology causes, Physiologi_,cal processess and phases as affected by salts stress.,Parameters for salts resistance screening techniques.

~2. Grop Production Physiology I Folier nutrition, fertilizers

their uses and limititions. JlMetabolism _ Recent information on Photo- synthesis and'.chemosyathosis, Respiration, nitrogen metabo~ism, lipid metabo_lism, secondary plant products, sulphur met~~lism, Interac~

~'

tion between photochemistry and activity of enzyme.Photoassimilation of organic compounds.. ;r

NOTE 1_ This course is of 60 lectures.Periods (60 minutes each) arc

Hence sixi •requ red.

(5+1)

-x-x-x-x-x-x-

cul ture media.

Cul turing of !\lg~ :

"

I.

,IDOT. : 4.31 hlga~e~Aal_~~p_~r::. _III (48 ~c~ ..lres)

'i(12 Lectures}"1. Defination, General requirements for culturing of algae,

I2. Preparatory culture ~d Isolation of algae 1_ Direct,

lII

cont ••10

Isolation, streak culturo, Nutritive solutions, Dilutioncultre technique, enrichmGnt culture, Biphasic culture,(Soil & water c~lture), solidculture.

3. Axenic culture, (Batteria fn:.e culture).4. Continuous culture.5. Synchronous culture.6. Mass culture: 3ubbling culture, strirring culture, shaking

culture, closed circulating system, open bubbling system,Different methods for cultivation of nitrogen fixing bluegreen algae.

7. Cultivation of algae in waste water.II. Algal Utilization 1 (10 Lectures)

1. lndustrial u~ 1- The kelp Industry, Agar agar, hlgina_tes, carrageenan & orhte products of marine algae.

2. Agriculturel uses 1_ Blue green algae as a bioferlitizer,organic manure, as a fodder for cattle, polutry feed,sea-weed liquid fertilizer, Biogas from Sea weeds.

3. As a human food 1- Fresh water algae as a food, marinealgae as a food.

4. Role of algae in Medicine.III. hlgal Phy~iology 1_ (14 Lectures)

1. Nutrition :- Nutritional rouqiremonts, macro andmicronutrients.

2. Nutritional types 1- Phototrophic, chemotrophic, Auto_trophic, Heterotrophic.

3. Photos~~esis 1- Definition, Energy levels in Chlorophyllmolecules, photochemical changes during absorption oflight, light and dark veaction, Glycolate opathway forcO2 fixation.

4. Respiration l- Defination types pathway of repiration.Kreh's cycle, Glyoxylate pathw,,-yscylce.

5. Nitrogen fixation 1 Defination, mechanism biochemistryof nitrogen fixation, factors affecting.

6. Nitrogen Metabolism 1_7. Movements and rhythams in algae.8. Calcification & silicification.e. Polarity & morphogenesis,

10. Bioelectric Phenomenon I-BiOlogical fuel cell11. Eioluminescence.

Cont ••l1

cont••1l

IV. Cytology and -9:.t-ochamistryof <11g"e l ,(12 Lectures)U Cytology l-

charophyta,2) Genetics of

& Blue-Grecn

1) Nuclear cytology of Chlorophyta,Bacillariophyta, Pheophyta, Rhodophyta.Green algae, Brown algae, Red algae,algae, plastid _ DNA, Algal virus mutation

in algae.3) Cytochemistry :_ Cell wall, mucilage, Fats,

Steroids~ pigments, Storage products, Extracellularproducts of Algae.

-x-x_x_x_x_x_x_x_

BOT. 4.32 Mycology & Pl?nt Patholo~Special Paper III.

"r. Phys~~logic<ll Plant Pathology(48 Lectures).

-L

Concept of plant disease, expression, Inoculum poten_tial, production, & districution, Mechnnism of infection.Effect of environment on multiplication 'of pathogens and

? outbreak of disease.Defence mechanism, disease resistance, Physiology ofdiseased plants.plant Diseases I

Important fungal, bacterial, viral, nematode & MLOmajor plant diseasos enc~untered in M3harashtra, Physical,Chemical, & biological control measures.

III. Medical mycology I

MyClCltic infections, dermatophytoses, & deep mycoses .•

IV. Seed Pathology, Ectophytis & exdophytic microbial associa~tions of seeds, Role of micro_org~nisms in storage ofseeds.

V. Market Pathology I

Pre & Post harvest micro-orgAnization.VI. Forest PAthol~ :

Forest diseases, managment and wood decay.VI!. Nutrition & Physiology of fungi :

" ,DispGrs~l & gormination of spores, growth, protoplast

isolation, morphogenesis, & fusion, Nutrition & metabolismof carbon, nitrogen, minerals, vitamins & growth regulators,fungAl dimorphism, Dual cultures.

cont••12

cont ••12

BOT I

(4-BLectures)

1. Ethnobotany I Scope and interdisc~plinary approaches.Abstruct and concrete Relntionships, methods, of ethno_botanical study. Ethnobotany in the c0ntext of developmentand conservation of resources, forest management andnational priorities, Ethnobotany of sacred plants of Indiasuch as Acgle, Mi'lrmo.los,species of Ficus, species of I'ci_mum, s~~talum Oalburn, cynodon dnctylon, etc. Plants inmagico-religious beliefs and sanskrit literatUre, Ethno_medicinal Plants in India, Tree <lmdPlorn, Mo-ti£.:l

Arboriculture t- Objectives in tree planting, selection oftree types on the basis of crown, shape, btanching habit,growth rate etc. trees suitable for roadside avenues,After care, need for manuring, maintaining shape and cavityrepair,

3. Floriculture Md land scape Gardening I seasonal <>nd perenni_al types of flowering plants, selection in colour Sa.emee, some

plants v~rieties fnr 10cCll cuitivation, cultivation of Roseand Jnsmine. Informal and founal gardens characteristicfeatures and symbolism ~f i) Japanese ii) Moghel and iii)English gi1rdens.

4. Herbaria an<!.~ta~~l...2!!:!:.der>~.: Herbari.iland Dotenicel gardenas multipurpose resource institutes. kinds of major her_bari.a of the World, Botenical gardens of the world.

•

NOTE. :- Special Paper - III is of 48 lectures i.e. 4+1 periods(4 lectures + 1 tutorial each lecture is of 60.minutes) -iare required,

-x-x-x-x-x-x-x_

Bar. : 4.3.i.}'lant..l'hysio.1oSU':.Special Paper-.E~I_(48 Lectures)_

1. Plant growth regulation concept of growth regulations.2. a) Phytochromones ,- Distribution, Niosysthesi.s properties,

metabolism, trilns location. mechanism of acti.on (at subce-llular and molecular lavel) Chemical and immuns assays.b) Naturally occuring growth regulaters (other than. Phyto-chromes) such as triacontarol Brassinolides and £usicoccia.c) Synthetic gro~~h substances.

1. Growth promoters.cont. ,13

.,•cont •• 13"....;, -, - " - ...•.. '.' .--~-------------nt

2. MorIJh"ctins.,~ 3. Growthinh",hitcrs.

d) Apl,lications c>f 1'1clllt growth regul2!tors in Agriculture,~~rticulture aod tissue culture.

e) Growth <:loddevelopment,

1. Concept.2. Hormonal requlntion. of growth.

3. Assessment of growth.4. Polarity.5. Uneven growth.6. Photomorphogenesis.7. Physiological & genetic b"sis of inproving photo~ynthetic

productivity.

3. UV.B. (280-320 nm) radiation effect on growth of plants andyield of crop plants. Response of plants to Uv.D. Radiation.

c

4. lli:;;y.e__",-uJ..!:..~ :"InVi\;r6" conditions" -li:Jbor<:ltory organistion. Aseptic

manipulations. nutrient media. Totipotency of'plant cells, ~ytypes of culture. ~rotopla5t culture isolution and purifica_t;.ioo__growth pattern in tisSUQ cultur",. or\!an?~e,:",sis.embryogenesis, Cytodifferentiation, Application of tissue cul_, . . .' .ture .i,n );;lasic.,andaI-'l;l~ed.aSpects of pl<lot physiology andplant propagation.

-x-x-x-x-x-x-x-x-x-x-,

c .." ,

•cont •• 14 . . ,

,"

i:•

" ~ •

•

,

,

,

cont •• 14•

DOT. 3._~.....£.l~$lcrl; ..ec!J.ric\1-0gy •: ..• - .~..2'9..~..h_l.?.~p.c.ti,c.<;lj..j..,.

,. ..•. \ . '...pr.ac:ticals)

,c~l1

( 2and assay.on domcost*c

. .waste water.,. .\

green algae (Qiofertilizers).., ,'"

C~tr~c ac~d fermcntat~onAlg<ll ~iomas~ ~roductiDnMass cult~vat~cn ,-,f Dlue:fz;*'act:icals) •

;';"1:1_'; •.. ':E~f.action an~ estimation o~ singl~i.o~r;'s.'mathod-. . .' '

'.f;oJ ,

Research',:'" .. .BiotechnologicalVisit to anyone of the

Mushroom Cultiv'l-tion ( 2 Practicalf? )'1"J '"e.''; .'En~yme, prqduqtion ane).asss-r. " .. . .a,UicroFrofB9a}iun,u~ ~conumi9,-,lfY ~~p~~t~~~ :~1t~~~i~~.In yi1;.r0lc\I.!tureof embryo to 7a.i;,.e..••v:.irRJ,e.~hyprir~'. r~F~oductio~ uf secondary metabolities in Vitro (2 Practicals) •

• ,' R' • ' ... ' ;~' ..•. :•• P.;.4'"rJ .'.'

NOTE ;-

Laboratories is essential.

,..•.A) C.F.T.R.I. - central food and Technological

Institute, Mysore.Research

B) N.C.L. _ National Chemical L"boratory, Pune.

•

C) Department of Botany and Biochemistry of Puna Un~versitYI

D) H.A. _ Hindustan AntibiotiCS, p.impr~, puna.

E) N.I.V. National Institute of Virology, pune.

F) LT.R.C. _ l.ndustr~al Toxicology Re'search Cent;:-e,Lucknow.

G) T.E.R.I.- Tata Energy Research Institute, New Delhi.

J) C.D.T. _ Centre fo+ B~otechnology, ~U, New Delhi.Madur«j Kamraj Uni., G.B. Pant AgricultJ,ure Un!. , --i

pantnager, Hvxayana, Agriculture Uni, .Hissar. M.Phu1eAgriculture Un1., poona (BGh, Mushroom projects) etC.

K} IhRI _ Indian Agriculture Research Institute,. New Delhi.

L) D.h.I.F. _ nhartiya hgro Industrial Foundation. pune.

M) I.M.T. _ Institute of Microbial Technology. Chandigarh.N) C.I.M.A.P. _ Centre for Ind~an Medicinal and l~omatic Plants,

Luc/Cnow.

•

1'1) N.D.R.•I.

I) C.O.R.I.

National Botanical Res~arch Institute, Lucknow.. .Central Drug and Research Inst~tute, Lucknow.

( Like this any more kncwn institutes which concerns Diotechno-log~cal work may alsO be visited )

cont ••15

•

•

BOT : 3.4 Pract~cal-r~ '. n'_ .• < _

1•.Cy~ad~~.~. .'a) External morphology of vegetatiTe- parts •.

I, Entire plant,o£.cycas__~~~~ip?_~, £. ,£~~ol~~-,,"l._g~As'l2..12.a~Foliage-c. ~jr~inalio.

or

'b) Megasporangiate -s~robili & Mega sporophylls ••

01)~oluta. c. cirq12~.' c. rumphj.i. Ce~t;..oz;'lmia!..~~mia&~~ephalartos.

-' , .-,

-'.

.-Gametophyt"'!O - enQ;l.r.QX9.ll!illY1, Microcy~ - free nucl"'"r-..-r:togc.

-:i. Zan'tia Ar•.hegonia & Proembryo. _.,_

oj .~erosporr:..!.1R.~_S_t:-.!'£llii.....&_.M.icrosporophytes' : c. ,.9.E::=cinalis, Zam_iafloridLina, Ceratoz"mia, Encephal~.!'~ ',

.ell,'-'

, ."',,

--'. ...",- .~,'.~,.

•

-." -.'.2,' £Y..~, ';-,Embryo.

-,e) ,Anatomy _'

1. ,T.S", Rachis & Pinnae of S:L?",~B &. .~a~;;;

-'2~ OJni:fer<'iles-& Tax1'-~'

-._,~US, C,,:~US.cupre~sus •..1~E.1!-~E-Eia_.'Ag~.h:!P_J;g.'i!o~"E!e''._Taxodi~.cQphalo~a~~'&Taxu~.a) -DoUblestilined p~rrnanent preparation~ a£,wood of any

."-"'- '-'two of the genera •... ".V

...-

- -:.'

b)- T.S. of stern one genus from:~..n.::P'alilIlly•.

6) T~S •• T.L.S. &'RLS, -0£ \,;Qod & preparation 'of artificialkeys ,~t least one gcnu~- from each family;'

d) Study of male cono.c:o -&-mi:crosporophylls ...&'":~croePOre:'l _ at--'-'..J.east-. One genus froll'l--eal::h frunilY-1'~

e) Female-cone-c -" -ovu-l::tferous .scales of',-~-Pi~.-.E..~,?_ucosug<)

Cupres~~, Th~ Ar<)cilria; CryPtomeria,Ta1S£..~_•.....!'2.d.2.~a_lJ:'.E~":'..r._

-f) -G,ulle-tophytcs& 'embryogeny ofPinue ;;.Archegonia, Proembryo_& suspensor.

I,

.,... - Qpet"les : :E=.E.he~..:;:&G-'not~nl:(ill H"bit & extorn~l morphology.b) Morphology of reproductive p~trr.

1. Mule Strobilus - Microsporophytes;- Pelle gr('[ins~2,' Fernnle strobilus.- .

•••.). Gametophytes & embryogeny Fem"l",' gi'lllletbpnyte of <';ne,turn.'d) Antltomy 1. T.S~ Stern•. ,

2. T.S•• T~L.S. & R.L.S. of wood~;

Pl,LhCODOTANY_ ...•_ ..... _ ..__. c(.Jnt•• 16

.L_,,-- !'Ei'.2.t.i.c.fl..l.sj_Morpholog1cQl & 2ni'.tomic~1 study ,f the following.

1. PsiloI)sida.

2. '!:y"c.op.sid<J.Stem-gener"

a) !:.&~:.oP.~'Ltf',-1}!,_r.i.n.c.~s.b) ~bYE~~(stem T.S,)

~ . .,,) , LCp'i_dodendE.9.!!_y.a~c~l<J.re.

b) L•.!"5'}e~oti~~!.

•

. ..' Leaf cushions - a) LepiC!.o~.fl2Eo_r:!._.b) ~P~~9~h}oi~Jslide~, m~terial & peels)

Roots - ~...wn5'.!"i"..£icuides (T,5. Peel)

!£'.S:~;Lh..,.."tio!! 0.I'J.~d.,!strobus-.!,k"..Ei;.Cl.~~"rpon~.

3. p.£.~P2~da.Stem genera - a) ~h8nop~1~

b) Arthr0Htys.

c) ~<',moden~S'..p'.•..

Annular.:.a.-- --~J.-z<)n$:ur~;,.•..Phyl..~ot~~-:!.'"..

Foliage _ a} ~}le.EOp!.'yll.'.~bl0101

•

-Fructific<ltion a) ~~~ostachys bJopp~yana.

b) C. americ<'.na.---_ ....._--- •

a) notr~op~cri$ tr~cnta. (stem & petiole)b) Q£.~ryopt;e.!"_~_.$1.1.$05". (fructHicaticm)c-t~X£'_l2.''iW1_S_i~.!.!.~~:5is.(stem T.S.)

d) ~iJ.2-1E.ojJ.t"eE.i;.?,~9.':l!.~islan<'!:i'?_,,_~_(mega.sporangium)

5. ~.g.lli};lles & fi.lic2.k~a) PS<lrpnius (T.S. stem'------b) ~o_~ecopter~ (fructification)

c) Marattiopsi!L~M<lcr<,2.<;;.a.n:::;:.

•

6. ~ ida sP5'.fJ!!.s.. .•_." __""""'ge - a) !,~eccp'~~.iJ!....

c) .!'!..~lll:'tlp~..eris~b} .:?I!.he11..oPte;:"i!l-d) g}~.OS~?l't":Els• .:,..•

Stem ganera -<l) ~ino~teris Oldh~a.. - --~---,,-b) !!!,_dullus~..!tJ-ompSoni•.

c) Y~~5~rR~~~_ (compression specimen)

7. CordQit~les. Coniferales, Ginkgoales, Pentoxylales &Denriettitnlcs.

Foliage _ 0101el

~CordaAt.os.~~r_oJ-'.h"y}_l~

Q;0.k5Lo~..dj.5!i.t..s...t" 1"-. ••

b} ~c.hY.l'.hJ':.t.1YI1l.d) r..~C!..e.!!..i.:..c:..0J~s.:ts •

,,=cont ••17

,

i;tem "-) £'?E.q..2.~_~.'?~'c) Fen~?z:.Y..:L~E~

b) C<l.rdiocnrJ:us.---_.- ---- -- I.8, A!!SL~o_sperom.

Monocot Cl)0)

PaJ,.~_~!'?E.z-b) S.~i.:.~p"\ll"!loxyl2..~Cycli~.J:!_9hC?j.~E.d.r_on.,d) Tr.1,.'=.o.92.,i tes.

Picot eJbJ0)

d)eJ

Dicct stem.Dicot leaf impressions.~1l.i,.9!1!9_~.!'.Q?.E.~S;:;hni",nthus.---------_.

, ~,

•

1, Andrews, B.N.2, Danks. B.P.

- 3. Dierhorst, D.W.~ 4, Dower, F.O.

5. C~pbell, D.H.6. Coulter, J.M. &

Chemberlain, C.J.7, OiJrrah, W,C.8. oe'le'tbryas, T.

9. Eames, A.J.

10, Fosters A.S. 8Gifford, E.M.~

~ 11. Goebel, K.12, Jenny, ;'.0.

13. M."heshwari, P, 8l3iswiJ.s,C.

14. Maheshwari, P••Konar, R.N.

15. Ogura, K.

15. Pant 0.0.&Mehril 0.

17. Parihilr. N,S.

- Studies in palaeobotany.

Evolution and Plants of the Past.

Morphology of VasculiJ.rPlilnts.Primitive land plants.

Evolution of land Plants.,..Morphology of Gymnosperms.

Principles of p"laeob0tany.

Morphology and evolution of fossilPlants.

Morphology of Vascular Plants.(Lower groups)

Compurative Morphology ofVascular Plants.

Organogrnphy of Plnnts.

Phylogeny and clnssificiltion of ferns.Cedrus.

- Pinus.

Comp<1r<,tive cantomy of Vegetativeorgans of Pteridophytes.

- Cycas.

Diology and Morphology of pteridophy-tes.

18. Parihar, N.S. An Introduction to Embroyphyta Vo1.II

cant •• 18

cant •• 1819. Pe<7lrson,H.H.W.20. Rashid, ".21. Seward, h.c.22. Smith G.M.23• Spore; K.R.24. Spore K.R.

25. Stewart W.N.

26. Taylor, T.N.

23. Walton, J.

Gneti:l1es.

An Introduction to Pterdophytes.

Plant life through Ages.

CrYr'togamic Boto:tnyVol.II

The Morphol;;>9Y of pteridOphytes.

The Morphology of Gymnosperms.

Palacobo any. and the evolutionof Pl<::nts.

Pal~cobotdny, an introduction tofossil Plant biology.Introduction to study offossil Plants.

-X-X-X-x-x-x-x_x_x_

SEMBS_TE._R_.,.'.'_

BOT.

Chlorophyta:

~..!..~_Practical 9..!!...!illecialp,!per A}:5l..<;l.£.!.(24 Practicals)

Volvocales :- Chlamydomonos, chlorogonium, Phacotus, GoniumPandorina, Eudorirm, Volvox.

Chlor£9~~~~ :- ChlorocDccum. Tetraspora, Chlorella, TrebauxiaTrebauxi~, Tetraedron. Chraeium, Chi:lrasiosiphon,Ankistrodcsmus. Selenestrum, Ooeystis, Dotry_ococeus, Coelastrum, Scenedesmus~ Pedi8sturm,Hydrodictyon, Proto siphon, Crucigenia.

Ulothrix, Uronema, Microspor~. Sphaeroplea,Cylindrocapsum.

~al~ :- Ulva, Entoromorpha, Sehizomoris. Monostrome.Ch8etophorales ; St-igeoclonium, Chaetophora, Drap~naldia,

Drapnn81diopsis, Fristchella. ColeochnetaeTrentepholia, Cephaleuros.

~?~~E~les :- Cl~dopho~a, Rhizoclonium, Pithuphora,Chaatomorpha, spongomorpha.

~dogon~~le~ :- Oedogunium, Dulbochactae, Oedoeladiurn!

~n!"matillea :- Sr-,irogyra,Zygncm"-, CyLlndrocystis,M9ugeotia, Sirogonium, Sirocladium, Cosmarium,Eucstrum. PleurotaOnium, Closterium.

Sip"honnles + Caulerp"-, Dryopsis, Dichotomosiphon, Coeium,Halimeda, Doergcscnia, Valonia, Neomeris,Accta-bularia, Chamaedoris.

cont ••19

"-'-cont••l9

Cl--/AROPHYTl,_._. NITELLl,.--~._-EUglenophyta :- Euglen"" Phacus, Lepocinelis, Tr~chelomonas.xan~ho~E~:- Vaucheria, DotryCium.£hr~~ophytu l_ Dinobryon, Synura.~cilluri~t~ :- Coscinodiscus, Melosira, Chaetoeoros,

Didulphia, Nistschia, Syncdra, Planktoniclla.Q!rof1aqcJlates :-Ceratium,Phaeophyt<l :_

Rhedophyta .:_

Ectoeurpus. Sphacelaria, Dictycta, Padin~,Stocchospermum, Sp"thoglosum, Dictyopteris,Colpomenia, Iycngaria, Surgassum. Turbinaria.Zoncria, Rhos~nvingic. L~minaria, Fueus,Cystosier~. Chnoospora.Porphyra. compsopogon, E~traehospermurn,Liager"',Seinia, Gelidium, Gelidi~lla,Gratcloupia. Graeillaria, Jani'", Hypnea,Rhodymcnia, Champi",,Ceramium, Caloglossa,l'eanthophor", Chondrus, Laurentia, Polysipho_nia, Aspargopsis, Halymnia, Botrycladia.. , .chroococeus. GleocapsiJ.,Gleothcce. Merismopedia,

•Aphanothece, Microcystis, Oscillatoria,Phormidium, Lyngbya, ~~throspirn, S~irulina,hUlosirn, Scytonema, Tolypotrix, Calothrix,.Rivuluria, Gleotrichin. Cyindrosperm~, Nostoc,Nostocopsis, Stigonema, Hapalosipho~AniJ.baena.

-x-x-X-X-X-x-X_X_X_

DOT '_~ ..2~.ctic<::~-:!!(on DOT. 3.32'Myeolgy and Plant Pathology Special

Papor-I)

Ceratiomyxa, Licea, Lycogal",,Trichia. Hemitrichia,Stemonitis, L~proderma, Physarum, Arcyria, Ful~g0,Cratcrium, Diderma Didymium.

Plasm diophora, Spongospora.

cont ••20

cant •• 20

Synchytrium. rhys~derma. SuprelegniG Achlyu,Dlpidium. },lbuge, Pythiwn, Phytopthori'!. Flasmopar<:t,perono-spera. Dremiu, $cler"spura,

Rhizopus, Mucor, Zygorhynchus, PilobolusEntomophther~, Choanephora.

Protomyces, Taphr.tna, Eurotiurn, Chaetomium, Erysiphe,Uncinula, Phyllactinia, Sph<:terothcca, Podosphaera, Micr~sphaera, Meliola, Phyllachora, Daldinia, Xylaria, Hyphoxylon,Claviceps, Balansia, Peziza, Pseudopcziza, MorchellaRhytisma. Uanca, Parmelia, Pyxine, Parodiella, Elsinoe,Dagnisiella. Hysterium, Pleespera. Leptosphaeria, Didymo-spharria.

Melampsora. Dasturella, Puccinia, Ravenoli<:t,Uromyces,Monosporidium, Cystepsora, Cerotelium, Olivera, Chaconia,Phragmidium, Hemileia, Catenulopsora, Endophyllum,Trichodium, Masseeela, G~leola, Hapalephragmicpsis,Graphiola, Ustilago, Spacelotheca, TolypospQrium,DOessansiu, Georgetischeria, Enthloma. Tilletia, }'uricularia,Calocera, Tremella, Polyporus, Perin Fornes. Schizophyllum,Lenzites. Ganoderma. Clavaria, Daedalia, Hymenochaete. Hexa-gonia, Sternum. Irpex. Hydnum. Ag~ricus. Cop- ainus. Lepiota,Pleurotus, Phallus, Dictyophora, Itajahya, Lycoperdon,Geaster, Cyathus, Nidularia.

Fusarium, Curvularia, Alternaria. Cla?esporium,Cercoapora, Dcltrania, Penicillium, Aspcrgious, Fhoma.Phylosticta, Pestalotia, Colletotr{chum', Ciliochorella.

NOTE: 1) Short local tours.2) One long tour.

-x-x-x-x-x-x-~-...Q..~_:_3.!_5 _

1I~_'?_s.J'.:'~..r.e9.i_a_l_.pra£tJ-S.3lS_-'-2.!..yra~t.i2~~sJ_1. Botanical description uf l'lants.2. Indentification of plant specimens upto species level (with

the help of Flora/Floras).

Frep<rratiun ofi) Idented Key

artificial dichotcmous keys ofii} Dracketed Key tYr~s based on di_fferent

cont ••21

.~-~~---------------------------------~---~---------••~-cont •• 21.

morphologic~lchar~cters.4. Study of J,ngicspermc filIllilies loco!.11yas well as

availdble in the region cover sing all the orders/seriesaccording to Bentham and Hooker's system.

5. Chemical testing of floral pigments in caryophyllales.curvembryeae and fecoidales.

6. Study of Vessel elements by maceration method (differenttypes of vossel elements).

7. Study of epidermal appendages and prepare a dichotmus keyto differentiate,

Students should undertake one short and one longBotanical excursion, submission of forty herberiumspec~cns alongwith tour report is compulsory.

-x-x-x-x_x_x_

by two"

acids

~~~~~. Practical II (on DOT 3.31)9E....E.l2£..ci~Pap~.F=-I~

(24 Practicnls)

Extraction and separation of organicdimensiolk"1l p"per chromatogr2.phy.Extraction and separation of sugars by two dimensional,2•

'.paper chromatography.

Extrnction and separation ofpaper chromatography.

,amonoacids by two dimensional

'j,.5.

-\... 6.7.

8.9.10.11 &

,13.

14.15 &

Est~ation of nscorbic acid.

Extraction and separaticn of alkaloids by two dimensionalpaper chromutography.

Estimation of oxalic acid.

Extraction and separation of liquids by thin layerchromatography.Estimation of Lignins.Estimation Polyphenols.Determine the activity of enzyme ployphenol oxidase.12. Colorimetric estimCltion of DNA & RN1:.Isolation of DNA from cauliflower.Isollltion of RNJ, from yeast.16 Isozymepilttern of peroxidase enzyme under underdifferent conditions using gel electrophoresis.

17 & 18. Isolation of Mitochondria fromplant mnterial &demonstration of succinic delydrogenaSC'uceivity •

• ';lOt •• 22

---- ,

cont ••22

19. Isolation of chloroplasts und demonstrnticn of Hill reaction20.& 21. Sepnr<J.tionof proteins on the basis of mol"'.'.llar

'Iwight using SDS - PhGE_ electrophoresis.

22. ColGrimetric estimation of nitrogen from plant materi<J.l

23. Estimation of aminu nitrugen frum plant material.

24. Extraction ond estimation of total alkaloids from plnntffi<Jterial.

-x-x-x-x-x_x_x_

DOT I _';I~!..!, Pra...ctic1l_1_.,?_~p_~.<:I.1 J_~V:~_l.9pmental..ll.~'2I}¥).( 24 practicals )

A. ~atomy ( 8 Prncticals )1, study of stomatal types from fresh preparation by

students.

2. Isolation and study of wood - elements by acid macerationmethod. preparation of pCrIni10ent slides by s't,udents. q

3. Diagnostic study of gymnosperm woods at least two conifer ~fer types by study uf Transverse section, Radi~l longitu-dinal section. Tangential section DoUble _ stninpreparation by students.

4. Diagnostic study Jf dicotyledon woods, at least 4 typeby T.S. and T.L.S. Double stain preparation by studgnts.

5. Study of Xylem parenchyma types permanent. preparationby students.

6. Study of abnormal secondary growth. 3 dicot types ; & imonocot type.

NOTE I Submission of permanet slide preparation at leastof 2 woods and 2 whole mount of wood maceratain is

B. Ernbryolgy (8 Pra..£!:!..cals,)

~necess~'

1. Study of the development of rnicrospor<lngiurnmicrospores,

male gdJlletophyteof angioperms. with the help of perma-nent slides.

2. i) Study of development stages of magasporangiurn (from 'l""!permanent slides) ii) Study of types of ovules(vertical sections from microslides) 3) Study ofmegasporogenesis. and female gemetophytess of angio-sperms, at least types. (from permanet slides).

4. Study of endosperm types and 2 midifications.

cont •• 23

~ )~5. study of embryogenesis. (from permDnent slides.6. Dissection and mounting of {al Globular embryo with hau_

storium in Cu~j._'L. seeds. (to hEldone by studlilnts).

b) Multiple embryos in Ci~~ seeds.

(8 Practicals) ,"

Bryophyte type.pteridOphyte type."

"

1, Study of pollen development, Tetrad typos, pollenunits (monad. diad, tetrad. polyad. pollinia)

2. Pollen spore preparation by acetolysis technique.,3 Slides angiosperm types.1 Slides gymnosperm tYi:Jo.11

3. Study of pollen morphology pot~rity. symmetry shapeside sporoderm stratification; aperture. N.P.C.

4. Pollon analysis from honey by acetoplysis.

5. Demonstration - Aorobiological study.

_N_O_TE:- Submission of five palynological slides is compulsory.

Plant embryology.The embryology of angiosperms.

Plant An<l.tomyVol. I and II.Anatomy of Seed plants.Plant Anatomy (2nd cd.).

Formation of wood in Forest Troes.Structure of Wood.Am introduction to embryology of Angio-sperms.

: Recent Advances in the Embryology of

Expcriment111 embryology of VascularPl;:mts.

RECOMMENDED Domes ,--_. -_.

'. Cutter. E.G. ,2. Esau. K. ,3. Fahn. A ,4. Zirnrnermamn. M.H. ,5. Jane, F.W. ,6. Maheshwari. P.,7. Maheshwari. P. and

~L Johri, n.M.

8. Johansen, 0.1,. I

9. Dhojwani, 5.5. , ,Bhatnllgar. S.P.

lO. Johri, D.M. ,Embryology of hngiosprms.Hand book of palynology pollan morpho-logy and plant taxonomy.Pollen-Development 1100 Physiology.'

Recent advances in Pollen and paioResearch Vol I. II and III.

, .Morpho1ogic1l1 encyclopedia of Palynology.Geophytology 7 (i) 1-23 and Prijanto.D.

cant •• 24

11. Johri. D.M. ,12. Eroltman, G. ,

13. Heslop-H<l.rrisonJ.

14. Nair, P.ll::.K.,15. !<remp. G.O ••••.!. ,16. Rowlry J.R. (1977 ) ,

---

cont •• 2.4

17. Rowley, J.R. and (1983) :J.S. RawleyPublishing Co.

Biology and ImplicGtion for ~breeding Elsvier Science

18. Rowley, J.R. (1981)

19. Rowley J.R. and :M,Jar<:li_Komlodi

: Norw Jour Dot 1 : 357-380

ObscrV<:ltion of one pollen g~nin by SEM.TEM & Lighy microscopy, ActR, ~ot.Acad.Seien. Hung, Torm.ts-22 (3,4) 449-461.

DOT :- ..._-

-X-X-X'.X-xx_X_X_)(_

4.5 (Practicals on DOT 4.21 & DOT.4.31)-- _-- -.---_.- ----- .._.._._-_._.~==---Algae Special (24 Practica!s)

1. Isolation and establishment of laboratory culture of Algae.2. Algal Diomass (Total chlorophyll, packed Cell volume,

fresh wt. and dry wt. J3. Algae of unsunl habits,

Epiphytes, epizoic.Epiphloephytes. Endophytes, algalaeroflora.

4•5.6•7.

soil and paddy field algae.Algae of polluted wat~r.Algae of puddles, ponds, lakes, streums, rivers.a) Algae of west coast and east coast of India.

(Marine plankton and seaweeds).b) Extraction of agar-agar and alginic acid. (Demonstration

practical) •

8. ~~r_a.22-a'!'y'si_:!.:P.H. Temper21ture, TUrbidity, D.O. CO2 BOD,COD, Nitrate, Phosphate, Alkalinity, Chlorides, TotalHardness. Silicates (any ten).

for alg0l cells.

of aminoacid of algae by paper

of carbohydrates of algae by

10.

ll.

12.13.

Quantitative studies of phytoplankton usingviz : (Simple drop method or HcamocytometerExtraction and separationchromatogr<lphy.Extraction and seperationpaper chromatography.Immobilization TechniqueCytological studies of :

standard methodsmethod (anyone).

J

£h.~r~, £iadophor"", !l-ll.i.~ocl..2.-nium.!,.J".1;..l}£ph~AOe0flP.Qnium andSpir..?.9XEaHy~pdic1<Y.on

N.B. :- Students should Visit atleast any onc of the followingResearch Institutes and submit the report.

1. C.F.T.R.L. - Central food end Technological researchInstitute, Mysore.

cant •• 25

cont •• 25

,~ 2. I.A.R.I. - Indian AgricUltural Research Institute,New Delhi.(NatiQn~1 Facility for Blue Gre~n Algae).

3. C.S.M.C.R.I. Central Salt and Marine Cultivation Research,Institute. Bhavnagar & Dwarka.

4•..CMFRS - Central !'\LJrineFisheries Research station, Mandaparn.5. MCRC

6. KIA

Murugappa Chettier Researoh Institute,Tharamani, Madras (~i3u~i~ culture)

Krishnamoorthy Institute of hlgology, Madras.7. NFMEGl,-National Facility for Marine Blue Green Algae.8. NIO - National Institute of Oceanography, GOa.9. RRI, - Rice Research Institute, Cuttack, Orissa.

"

Lucknow '(U.P.)10. cnv,p - Central Institute of Medicinel & Aromatic Plants

••

National Botanical Research Institute, Lucknow.

Nadu hgaricultural University. Co~atore.National Environmental EngineeringResearch Institut~, Hyderabad. Nagpur.

- Contral Drug Research Institute, Lucknow. (U.P.).,- National Research laboratory for conserv~tion of

cultural property, Lucknow (U.P.).

11. Tomil12. NEERI

,."- 13. NDRI

14. CDRI15. NRLC

(nOT.3.31 • DOT 4.21 and DOT 4.31)

Algae Structure and reproduction.A. Biology of marine Algae.Aquatic ECOlO>!y•.;Phytoplankton.Seaweeds & their.uses.The l~lgae.

Diology of Dlue-green Algae.M"rine Dot,any.Marine Botany. I,Internationa,l.sympsiurnonTnxonomy of J,lgac.•

- Diolo'JY of the Rhodophytn.The Structur~. and production of~lgae Vo~. I & II.Chromosomes of 1.1gae.

cont ••26

1. Bold, H. & Wynne If.J.2• Doney A.D.

~ 3. Brain Moss•~ 4. Doney A.D.5. Chapmi'nV.J.&. Chapm~n V.J.

C& Chapman D.J.

7• Carrs and Whitton8. Dawes C.J.'. Dawson E.Y.

10. Desikachary T.V.

11. Dixon P.S.12. Fritsch F.E.

13. GOdward M.D.E.

-~-c,nt •• 215.

Stephanson & Stephenson - Life betw~en tide marks.

14. Irrine D.E.G. &

43. Welch P.S.44. Wetzel R.G.

45. Wetzel R.G.

Systematics of the.green algae.

Syrnpsium on Algology.Symposium and plants.Genetics of algae.Algal Physiology & Biochemistry.The ecology of.rocky shores.

~ Phycology.An Introduction to the Alg~e.

- Seaweed Utilization,Alg<l.e.

The Algal culture.Methane Genaration From waste.Sewage treatment in hot climate.Sanieativn in Deloping Countries.Rural technology.Ecolo~ of Inland Waters & estua_ries.

- Selected papers in Vol. I & II

Current trends in limnologyVol. I & II,

Limnologyof thermal; springs.- Siamass Energy.- Fresh water Algae of United statesAdvances in Limnology.

- Manual of phycology.

Algal Fhysiology & Biochemistry.- Algae & their life relations,

fundamental of Phyology.Ecology of algae.Cultivation of Algae.Limnologicnl Methods.Limno loo;-y •Limnological analysis. (1991)Limnology ea. (1983)

SteWllrt W.D.P.

Tilden J .E.

Saha S.K.Smith V.Smith G.M.Singh H.R.Smith G.M.

Round F.E.Venkatarnman G.S.Welch P.S.

H.John. D.M.

15~ Kacharoo P.

16, Krishnamurthy R.17. ~win18. Lewin19. Lewis ..T.R,20. Lee R.E.21. Morris I.

22, Newton23. Prescott G.W.24, Pringshim25. NAC

26. D.•Mara27. O<,cey A.28. ReddyM.

29. Reid and Wood

30. Rosowski J.R.Saha & Porker D.

31. Shastree N.R.

32.33.34.35.36.37.38.39.

1. Desikachary. T.V.2. Gon'ilalves. E.3. Iyengar, M.O,P.4. Misra J.N,

5. Philiposo M.T.

- Cyanophyta.•.•Oedogoniales.

& LEsikarchay T.V. _ Volvocalcs.PhaGophycea",.Chlorococculos.

6, Ramanathan. K,R.7. Balukrishnan. M.S.

Ulotrich<llGs.Rhodophyt<l.'

cont. ,27

cdnt,,27

8. Randhawa, M.S. - Zygncm~taccac,9. Pal, D.P. 1100

Sundarlingum etal _ Char~ceae,10. Venkataram~n, G.S,_ Vauchcriaccac.

"

<

1. Phykdlll2. Journal of Phycology.3. Phycologia.4. Cryptogamie hlQologie.5. Japanese Journal of Phycology.6. Dritish Phycologica Journal.7. Dotanical MGrina.8. Hydrobiologia,9. Archive Hydrobiologia.

,

-X-X-X-x-x-x_x_x

(24 Practicals)Isolation and reinocullltion,of fungal pathogens.Isolation and reinoeuliltion of Bilcterialpathogens.Isolation of Nitrogen fixers and in vitro growth of nodules.& 5. Evalution of minimcm inhibitilrycinccntration (MIC)

of fungicides (two practicals).Pathology of seeds.Pathology of fruits.Isolation of soil microorganisms.

9.& 10. SCreening of ~croorganisms for primary & secondary meta_bolites.

11. & 12 Citric acid fermentation & assily.13. Penicillin bio-assay (organism _ Bacillus),14 & 15 Alcohol fermentation &" distillation.16. Spawn preparation & mushroom cultivation.17. Study of diseases caused.by Buctcrial viruscs MLO's &

nematode (one form each group),'18. Study of discuses caused by Mastigomycotina & Plasmodiophoralcs

(lInythree).19. Study of discuses caused by Ascomycotina (Any three].2J. Study o£ diseases caused b,'Dasidiomycotinu ( ~,ny three).21. Study of disease caused by Deuteromycotina (hny three].22. Diochemica1 studies of diseased p1unts chromatography

(Sugars & attlinoaciGls),2~. Diochem~ca1 studies of diseased plants poly phonols!

enzymes! RNi,.Dty"

,..'--

1.2.3 •4.

'.7.8.

23. Biochemical studies of diseased Plant cont.,28Proteins.

Alexopoulos C.J. 1962.

c"nt •• 28.

NOTE IVisit to a fermentation industry, Research institute,Agricultural university etc. Long & Short tours forCollection of Phytopathogenic organisms are essential.

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\li<tlc,M~E:.} ~1Jg.67;~-[l'.~j:rnL~y" .,-)f;H:rl:chl;n£ '.jjf1'i% if).)!;" 'ifd;hVdlf;;~'l-{61d:c.iUT.>.J<):t' •.lp.U • :, ~j,.'to~n',yl"::, ••'ldl"a

Hanlj,I),',FJ • .:{.- l'99D.,. _'IlTlustr<lted g~~~~1:of J\s60J;yl~te:s;'l'PS~f.-'J!,ress•. ,;) '-Hat'iEly '~J••r;;' ~l9'l¥,::b:£;~"iri3'i6fuj,1J6~t,d~~~rh1~~:1~r~,~)p~~.~e~~~l;d

.r, ,hG.'c1, .,,~.:;:r:l'l, .1nU 8'iii"l' ~,I.-'r>nd.cE'.i~Jjt,._ r "'! '-'.' ':'''''"''''-', -'J" ,]-',"T' "_••. 1. .lKl on ..- ~_'~ .~.- ••• ,- ~ ,..•..... . . ~- -., . .'. ,'. ) .' -'- .... '.'"• - '. -" .00C' .y,c>l ..~},':Mr.iJ\T'';>'':u',;,LdCJ:::>cI "'1," .3: T~LILfHHurley & Sl'nith' t98;3.' _MY~Qrrhi,z91',SyU.p.fQ~_.:i.s. '. .

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.n"i)nc,l bJ.l .:0", f"lGlc.t.N ,';::,~r. .,H •.• .tr:r..~tl:-- .1'0 d::;_~n" d!';ln~:.. cq7,t!,!-, ~-. t .tfMS•••~Wl,Lm1don;::-::':t=~nl.l

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cont ••36

- "~""""" ." - -

cunt •• 36

13. Dio 10gic,,1 Abstr<'.cts. U.S .l,.14. Sydowia.

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22. C.M.I. Description of Pathogenic Fungi and Dacteria.

-x-x-x-x-x_x_

cant ••3?

dbS./-

cont•.37\?:.aT 1;' S-

Angiosperm spe~al practicals (24 practic~~

1-4) Anatomical characterization of wood (T.S••T.L.S.,R.L.S.and muceration) - qualitative and quantitative (ony four)

5) Preparation Of actificial keybasis of improtant anatomicalcharacters.

,to distinguish woods on,and physico - Chemical

the

6) Anatomical analysis of reaction wood knots,7) Anatomical examination ••f secondary thickening in

Monocotyledons. (anyone)8) Histochemical examination of dicotyledons woods (any three)9) ~catiny the polysacchides

endosperm (any two).and proteins in dissected

- . :10) Dissection and mounting of multiple embryos.

~ 11) Dissection and exposure of peculiar embryologicalcharacters Bicelled,pollen,Hypostase,free nuclearendosperm. suspesor haustoria. I

12) To ~~udy Nodal Anatomy (different types of nodes)..

. .13) To study of Pollen units in mimosae.1Q) T~ s~dy of Pollen units in hsclepidaceae and orchidaceae.15) ~ study of Pollen units in Cyperaceae.16) -'!'otest the Pollen 'fertility.IT)) To study of Pollen Morphism.IO} Pollen analysis of honey's locally available (unifloral

'\1" multifloral) ',-Biochemical analysis of pollen grains.P.lynotaxonomy of a selected taxa of LeyuminosaelAcanthaceae of any other suitable taxa.To study the development of male Yametophyte.~ stwdy the course of Pollen tube through,stigma styleand ovary to ovale in naturally pollinated plants.Pollen germination in Vitro.P.llen preparation techniques.

i,,)20)~

21)22)

23)24)

Notel. Eaeh practical 1s of 4 clock hours duration.

~2!~~E~~-~E~~t~!_~~~~E~~~~_~~£t=_~~9_E~E!£~!~~1~.•( Bo. 3.33, 4.25, 4.33 )

1.2.3.

Arber !I..Arb",_ A.Bailey I.W.

Sixe and formation in plants.MOnocotyledons.Contribution to plant anatomy.

contd.3P

-

cont •. 38

4. Benson L.

5. Benson L.

Plant classification.

Plant taxonomy.

15. Deb1QrQn R.M.T.and CliffordH.T. (1982)

Sy~temat1c embry~logr of ~ngiospcrm.Principles of Angiosperms Taxonomy.

Families of the monocatyledons and others.The monocotyledons- A comparative study.

The embroyology of Anagiospermstreatise.

Ecological strategies of Xylem ecolution.Ghosh - Indian Hoods Vol.I

plant TiiXonomy.

The evolution and classification offlowering plants.Plant Taxonomy Vol. I and II.

Bhojwani S.S.andBhatnager S.P.Carlquiest s.

Choudhury and

Core E.L.Cronquist l\..

7.

6.

8.9.10.

CUtter E.G.

Davis G.!>.Davis P.R. andHeywood V.H.

14. Dehlgren R.M.T.

11.12.

13.

./

,

Erdtman G.Eames A.J. Morphology of the angiosperms.

Handbook of palynology, pollen morphology,and plant taxonomy.

Fahn-A. Plant Taxonorr.y.Frogin I. Textbook of pallen analysis.Hall M.A. Plant structurefunctionandHeywood V.H.(Ed.) d i(1968) Me ern methods n planttaxonomy,

~cademic press, London.

16.

17.

18.

19.20.

21.

22. Hooker J.D. h Sketch- of the flore of British India.23. Hatchimson J. (1969)- The evolution" classification of r

flowering plants.

I•Pollen development physiology.

-----

Families of flowering plants vol.I & II.Evolution and phylogency of floweringplants. ~Glimpses of Indian Ethnobotany oxfordand ISH publising Co. New Delhi. •Methods of Approaches in Ethonob9,tanySociety of Ethnobotanists, Lucknow •.

24. Haller J.M.

25. Heslop HarrisonJ. (ed)

26. Hutchinson J.27. HUtchinson J.

28. Jain S.R. (198l)

29. Jain S.K. (1989)

30. Jain S.K. (1987)

". Jain S.K. (1991)

A manual of Ethonobotany Scientificpublishers, maan Shavan, R~tanadu road,Jodhpur-.34200LDictionary of Indian folk medicine andEthnobotany Deep publications,A-3/27 A, DDA plants, puschim Vihar,NEW DELHI-11J 063, (India).

32. Jain S.K. (1991) Contribution to Indian Echnobotany.Scientific publishers, 5_1> New pali road,P.B.91, JODHPUR-342 001. (India).

Contd.... 39

------------ --------••,.~-----,,----'I

Contd ••• 39

33. Jain S.K. ,Sinha B .K.&

Gupta R.C. (1992)

". Jones.35. Jain a.K. and Roo R.R.

36. J,.me F .E.

37. Johri B .M. (ed. )

38. Jodhri B.M. (ed.)

39. Keneth E.F.40: Kunit,.,kiK. (1977)

Notable plants in EthnomedicineIndia Deep publication Delhi.

pL;mt Biosystemi'ltics•.H~ndbook of field and Herbariummethods. . jE

3tructure of Hood.Experimental erobryology ofvascular pl",nts.Embryology of angiosperms~Ecology and Resources management.plant systemic and evolution.

41. Kuijt J. The biologyplan~s.

of parasitic flowering,42. LAwrence G.B.H.

(, 45. Malik C.P. (cd),

46. Malcruny D.L. and47• Hetc<llfe C.R.cnd

'". Metcalfe C.R.

49. Mitra J.N.

50. Nair P.K.K.

51. Nair P.K.K.

43. Maheshwari P.

44. Maheshwari P.Johri B.M.

T~xonomy of vescular plants.,)\n Introduction to embryology of!~ngiospertns.

and Recent odv~nce in the embryologyof ,~gisperms. 1Physiology of sexual reproductionin flowering plants.

other -. Biotechnology "nd ecology of pollen.

Chalk.-Antomy of the dicotyledonsvel. i and IX. 1"-J\ntomy of. the monQco.tyledonsVol. V.,Cyperaceae~,An introduction to systematicBotany and ecology.hdvances in p~~nt science.Recent advances:in pollen sporeresearch 'veil.l, II and III.

". Nail v .N.

53. Prione P •.P

54. pandey B.P. (1989)

55.

56.

57.

58.

60.

61.

Redford ~.E.and others.Raghavan V.

Rao C.V.Randle ;,.B.

Robilrds '..,.

Randhawa G.S.andMukhopandhyay ,-,.(1986)

S"'greiya K.P.

$culthorp€ C.D.

Taxonomy of Angiosperms.Tree mairitenance.Plants for Human kind sacredplants of India.

Vascular plant systematics.Experimental embryogenesis inVascular plants.,prote"ceae.The classification of floweringplants, vol. I and II.Dynamic aspects of pl~nt ultra_-structure.Floriculture in India, Alliedpub. (p) Ltd. NEW DELHX,Bombay.Ornumantal trees thexr plantingtmd cnre. i

~'Biology of Aquatic Vasculnr pl~nts.

63. Shivannn K.R.andJodhri I3.M.

64. Sporne K.R.

The angiosperms pollen structurennd function.The mysterious origin offlowering plants.

Contd ••• 40

ContC1••• 1\0

Plant T"xnnomy and BiQsystematics.pc-lten, Biology BiochemistrymFln<lgement.

= Gr~wth "nd organizBtion in pl~nts.Essay in Plant Taxonomy.,Aquatic angiosperms.

I Text book of Bote-ny_: Introduction to principles of plant

TAxonomy.

65. Stacs C.".a(, Stantey R..by Inis

K.H;F.

n. Stewi3rd F.C.63. street H.E.

6;. Subrahmi'.nyam K.70. Swingle D.B.

7<. Sivarajan V,V.

";'. sporne K.R. I The Morphology of 'l\.l1giosperms, thestructure eVOlution of floweringplants.

73 stebbins C.L. {1974}I Flowering pl'wt Evolution i'lbove thespecies level.

-4, Takht~hanA. Floweringplants origin and dispersal.7,. Tiyagi and Kshetrapal. -; Taxonomy of Angiosperms.76 Thorpe Trever .:".C. : Pl."nt Tissue culture I Methods and

applic<ttions.

x-x-x-x-x--------- ./

304.5 on BO4.26 :md BO4.30 (24 practiccl1s)---------------------------------------------Rl:;ccn-u-wm:rBt> FhlO¥8-

2. Donner, J.& VArner,J.E.

3. Brynnt J.,i.

; Laboratory investigations in cell[l Biology.Pl~nt Biochemistry ~c~demic Press,lIewYork.

; MO:ecular lISpoCts of gene expre--ssi.on in plilnts.

4. Burgess, J. : An In"'roduction to' plant oeL.devolop;nent.

Coonbs, J.Hall,D.O.Long, S.P.~nd Scurlock, IM.n.

5.

6.

7.

8.9.

Butenko, B.G.

3urton, W.

David, LN.

De nobertis, D.D.M.i'Bd EMTDe.Roberti.

1 PIHnt tissue culture Rnd",lantmorpho_ge~~sis.Techniques in Diop"ductivity andphotosynthesis Ox~rd NewYork.Toronto Sydney F,.J.nkfurt.

: Post hArvest physblogy of fruit an".ndvegetFlbles.Piochonictry of nuceic acids.

I Cell and MoJo~l~r 3ioJogy.

•,~

Epsrein E.Mineri',lnutrition of plants.

10.

11.

12.

Dodds J.H. HndRobert,L.'t<. ; Expc~J.mf'nts :n tissu<'! ("\\l+-:tn_".

Ed~ard5, G.&Walker,D.1 C3-C4mecha~smand cellular and- environmDn~': regulco_tion, of

photosynthes:s.I principles run~properties John wiley& ~ons INC. N~wyouk, London.

13. Gibas, M.and L,\b<ko.E.: Ell'-'yclopi'.cdi,-,of plant physiologyvol. 6,PhotQs",rpthesis IIspringerverl",y Be:.lin, Hcidelbc:.g.

,Contd ••• 41

, ----------~-~-~-------

• Contd..... 41

H. Giese,,",. • Cell physiology (Elns)•15. Gauen, H.G. • Inorganic plant nutrition.

16. Gunning. B.S. end • Plcmt cell Biology <:Iudul trastru-steer,M.W, -ctur"l approil-ch.

17. Grahum, C.F. end • Developmenti'll contro! in AnimalsWareing P.B. i;lTIdplants.

28. M<:Icmillion J.

•

metacrolism

Tissue culture.

I Principles of 3iochemestry.; plant growth and development.I Physiological ecology responses ofplants to environmental stressesAcademic, press New Yank, LOndon •

t Gencs Wiley-estcrn Ltd.I Gene Expression vol. 1,2,3, Wileyinter Science,

I Encyclopaedia of plant Physiology,New series vol.9 HOllllllnCLlregulationof plcmt development.I springer verlag, Berlin Hcidelberg New York.

•Plwnt cell structure and2nd London, New Youk.plant structure, function &",daptIJ-tion.

l Chloraphast metRbolism.I Cell Biology.

•Lewin • j

Lewin.

Halliwel,King D. ,Kruse, P.q:. andPatterson/M.K.LChninger,A.Leopold.Levitt, J.

Hall, J.L.Flowers, •T.J,and R.M...Roberts ••,Hnll, M.A. \

23.24.25.

20.21.22.

19.

18.

•l

29. Mettle, R.H. t Lytic compartment of .plant cells.

30. Marcelle, R.Clijsters, I Biological control of photosynthe-H.Vnn poucke M. _sis-martinus Nishoff publishes.

31. Morris. I secondary metabolism in plant cellcultures.

L.ill1dl,sbinall,

pilet, pau~\ EmilePreston R.D;pitt D.pitt D.

•

•

I stress physiology in crop plantsWiley interscience.

t Plant growth Regulatirs NationalAcademy of Sciences India GoldenJubilee Comemoration volume.Biotechnological applications of plant,

•t Physiology and Biochemistry ofdrought resistance in plants.Academic press New York.plant growth regulation.

I physical Biology of Plant cell walls.I Lysosomes and cell function,-I Lysosomes anct function.

Molecular Biology of cellmembrances.

L.G,

!;I.andR.C.

Quinn.

PaleyO.

MusselsstaplesNickell

35.36.37'-

36.39.

34.

40. Raghavan V,

41. Rainert J.

I Experomental eITbrybgenesis invascul~r plants.

t Results and problems in celldifferentiation Vol.l0

Contd.. 42

-conta... 42

Chloropl"sts.------------J. and 3ajaj,Rainert

Y.P.S.Taylor.43.

42.1 Plant cell tissue organ culture.I Molecular Genetics Vol.Ij\cadcmic Press.

44. Terms A and Arron M. I Encyclop~edia of plant physiologyvol.S Photosynthesis I. Springerverl~g Berlin Heidel Berg.

43. W",reing P.F.

46. Wr,,:eing P.P. endPhilips I.D.J.

47. Watson J.D.

48. White P.B.

Plant growth substances.

f The control of growth anddifferent1~tion in plants.

: The molecular Biology of gene W.H.Benyamin anrl co.

I The cUitivation of animal andplant cells.

X-x_x_x-------

~Q!~_~~~_Er2E~!E~!_~~_i~~_~Q!~_~~~i_~_~Q!~_~~~~L(24 Practicn!s)-------------

01.

02.

03"

06.

05.

07.

08.

Determine activity of RUBI' case in C3 ,C & CAM plantsby spectrophotometric method (Leaves of spinach & Archisof sugercane and Aloe of Dryophllum)

Determine the activity of PEP case in C ,C & CAM plantsby Spectrophotometric method (spinash o~ Arc~is, maize orArchis, meize or sugercane, Aloe or Bryophyllum.)

41 E.timation of the activity of nitrClte reductase.(Leaves of any legumini~us plants.)

Deturmine thn activity of emzyme glycolate oxidase inC3 & C4 Plants. (leaves of anyone C3 & C4 plants)

Deturmine activity of lipase by spectrophotometric methodduring various stages ~f germination in oil seeds(Ear_thamus. sunflower, Ricinus etc. anyone.) -Det ermine the activity of enzymes oatel,ae and perovidaseand study the invintro effects of salts (Nacl, Na SOKCL, MgCll etc.) on the activities Of the enzymes2 4(Leaves 0 Ipomoea, Caranthamus, spin~ch etc. anyoneplant meterial\Estimation of proline from plant pnrts under control andstress (drough~ and sult) cond~tions.

09. EJII'timationof free and bound ~minOucids form plantmaterial (Leilve~of lettuce a~ of any leguminious plant)

10. Study of change ~n orgRnic ci~stituents during senescencein leRves.

11. D ro"ssy fer nux:.n.12. CUlture of plant ::issues invi::ro.

Contd ••• 43

.r-----------------~.~.-__;;:_-~

14.15.16.17.18.

19.2021.

22.23.

t,'24;

, .,'~ Contd... 43

13. Assessment of growth in invintro cultured cells tissues.Study of stomatal physiology.Study of deficiency symptoms.Effects of deficency on chlorophyll biosynthesis.Effects of GR3 on activity of ~nykase (bartey)Changes in orgr.nic constituents during ripening offruits.Changes in organic constituents during flowering.Estimntion of proteins by lowry etal method.

Estimation of c0rbohydrates (reducing, non_reducingsugars, total sugars and starch.) iDemonstration of photosystem II activity. II

Determine the activity enzyme IAA oxidase.Study of suspension sulture.

THE END.

gsb./_

I.1

,I,

II----