Supplementary information for

Variations in microbial community and ciprofloxacin removal in

rhizospheric soils between two cultivars of Brassica parachinensis L.

Xian-pei Huanga, Ce-Hui Mo a, Jiao Yua, Hai-Ming Zhaoa, Can Menga, Yan-wen Lia,

Hui Lia, Quan-ying Cai a,*, Ming-Hung Wong a,b

a Guangdong Provincial Research Center for Environment Pollution Control and Remediation  Materials, School of Environment, Jinan University, Guangzhou 510632, China

b Consortium on Health, Environment, Education and Research (CHEER), and Department of  Science and Environmental Studies, Hong Kong Institute of Education, Hong Kong, China

Supplementary materials include the following:

Total pages: 19; total procedures: 1; total figures: 3; total tables: 9;

S1 The procedures of CIP analysis in soil sample

Fig. S1 PCoA of bacterial community in rhizospheric soils of the two cultivars based on the

weighted UniFrac distances at the OUT level at 97% identity

Fig. S2 PCoA of fungal community in rhizospheric soils of the two cultivars based on the Bray

curtis at the OUT level at 97% identity

Fig. S3 Average well color development (AWCD) of rhizospheric microbes of the two cultivars

Table S1 Individual carbon sources in Biolog EcoPlateTM

Table S2 Relative abundance of bacterial phylum in rhizospheric soils of the two cultivars (%)

Table S3 Relative abundance of bacterial order in rhizospheric soils of the two cultivars (%)

Table S4 Relative abundance of bacterial genus in rhizospheric soils of the two cultivars (%)

Table S5 Relative abundance of fungal phylum in rhizospheric soils of the two cultivars (%)

Table S6 Relative abundance of fungal order in rhizospheric soils of the two cultivars (%)

Table S7 Relative abundance of fungal genus in rhizospheric soils of the two cultivars (%)

Table S8 Rotated component matrix for the microbial carbon sources utilizations in the Biolog

EcoPlate assay

Table S9 Mean AWCD of each carbon source in the Biolog EcoPlate assay

S1 The procedures of CIP analysis in soil sampleThe procedures of CIP analysis in soil sample were conducted following our

previous method published in Science of the Total Environment (Wu et al, 2014), with

small modification.

1. Soils sample extraction and cleanup

One gram of soil sample was placed in a 10 mL centrifuge tube and extracted

with 5 mL of 50% MgNO3 aqueous solution containing 4% aqueous ammonia. The

tubes were vortexed (XW-80A, Hai men, China) for 10 min and extracted triplicate in

an ultrasonic bath (KQ-250E, Kun san, China) for 10 min. Then, the extracts were

centrifuged (HC-3018, Hefei, China) at 4500 rpm for 5min. The supernatants were

concentrated to near dryness using a rotary evaporator (RE-2000, Shanghai, China),

and then was purified by Oasis HLB extraction cartridges (3 mL/60 mg, Waters,

USA). The cartridge was conditioned with 6.0mL of methanol, followed by 6.0 mL of

Milli-Q water. The flow speed was set at 1.0 mL/min. The extracts were loaded onto

the cartridges and then rinsed with 6 mL of Milli-Q water and dried for 5 min. The

cartridges were eluted with 3 mL 1% acidified acetonitrile at a flow rate of 1.0

mL/min. The eluate was collected and concentrated under a gentle stream of nitrogen

gas to about 0.1 mL, and then dissolved in 0.1% formic acid aqueous solution and

0.1% formic acid acetonitrile (90/10, V/V) to a final volume of 1.0 mL. The solution

finally was filtered through 0.22 μm syringe filters (Tianjin, China) for instrument

analysis.

2. Instrument analysis

The measurement of CIP was conducted using a high performance liquid

chromatography–tandem mass spectrometry system (HPLC-MS/MS, Alliance 1100 of

HPLC, AB4000QTRAP of MS, Agilent). Chromatographic separation was achieved

using an Agilent Eclipse Plus C18 column (5 μm, 2.1 × 150 mm). The mobile phase

was acetonitrile: water (10/90, V/V, with 0.1% formic acid) at a flow rate of 0.2

mL/min. The column temperature was 20 °C, and the injection volume was 5 μL. The

instrument was operated in positive ion (ESI) mode for multiple reaction monitoring

(MRM). The desolvation temperature was adjusted to 600 °C, ion source voltage was

set at 5.5 kV, curtain gas was set at 0.14 MPa, atomizing air pressure was set at 0.41

MPa and dry gas pressure was set at 0.35 MPa. The collision pressure level was high.

3. Quality assurance/quality control and data analysis

Blank spikes, method blanks (solvent), matrix spike duplicate and sample

duplicates were routinely analyzed with soil samples. The standard deviation of

duplicate samples was less than 16.2%. The mean recoveries of CIP in soils were >

82.3%. The correlation coefficients (R2) of the calibration curve (0.5 to 1000 ×10-3

mg/L) were > 0.999, and the relative standard deviation (RSD) for all analytes was <

11%. The limits of detection based on a signal-to-noise ratio of 3 (S/N > 3) ranged

from 0.001 to 0.003 ×10-3 mg/L, and the limits of quantification ranged from 0.006 to

0.013 ×10-3 mg/L.

The concentrations of CIP in soils were reported on a dry weight basis. Statistical

analyses including one-way analyses of variance (ANOVA) followed by Duncan`s

multiple range test (α=0.05) were performed with Microsoft Excel 2010 and SPSS

19.0.

Refrence: Wu XL, Xiang L, Yan QY, Jiang YN, Li YW, Huang XP, et al. Distribution and risk

assessment of quinolone antibiotics in the soils from organic vegetable farms of a subtropical

city, southern China. Sci Total Environ 2014; 487: 399-406.

Fig. S1 PCoA of bacterial community in rhizospheric soils of the two cultivars based on the weighted UniFrac distances at the OUT level at 97% identity

Fig. S2 PCoA of fungal community in rhizospheric soils of the two cultivars based on the Bray curtis at the OUT level at 97% identity

Fig. S3 Average well color development (AWCD) of rhizospheric microbes of the two cultivars

Table S1 Individual carbon sources in Biolog EcoPlateTM

Serial No. Substrate Chemical formula1 B1: Pyruvic acid methyl ester C4H6O3

2 A2: β-Methyl-D-glucoside C7H14O6

3 A3: D-Galactonic acid γ-Lactone C6H10O6

4 A4: L-Arginine C6H14N4O2

5 C1: Tween 40 -6 B2: D-Xylose C5H10O5

7 B3: D-Galacturonic acid C6H10O7

8 B4: L-Asparagine C4H8N2O8

9 D1: Tween 80 -10 C2: i-Erythritol C4H10O4

11 C3: 2-Hydroxy benzoic acid C7H6O3

12 C4: L-Phenylalanine C9H11NO2

13 E1: α-Cyclodextrin C36H60O30

14 D2: D-Mannitol C6H14O6

15 D3: 4-Hydroxy benzoic acid C7H6O3

16 D4: L-Serine C3H7NO3

17 F1: Glycogen (C6H10O5)n18 E2: N-Acetyl-D-glucosamine C8H15NO6

19 E3: γ-Hydroxy butyric acid C4H8O3

20 E4: L-Threonine C4H9NO3

21 G1: D-Cellobiose C12H22O11

22 F2: D-Glucosaminic acid C6H13NO6

23 F3: Itaconic acid C5H6O4

24 F4: Glycyl-L-glutamic acid C7H12N2O5

25 H1: α-D-Lactose C12H22O11

26 G2: Glucose -l-phosphate C6H13O9P27 G3: α-Keto butyric acid C4H6O3

28 G4: Phenyethylamine C8H11N29 H2: D,L-α-Glycerol-phosphate C3H9O6P30 H3: D-Malic acid C4H6O5

31 H4: Putrescine C4H12N2

Table S2 Relative abundance of bacterial phylum in rhizospheric soils of the two cultivars (%)

TaxonCK T1 T2

Cutai Sijiu Cutai Sijiu Cutai Sijiuk_Archaea;p_Euryarchaeota 5.99±2.54 b1) 4.06±3.05 ab 0.96±0.53 a 4.99±2.05 ab 4.09±3.91 ab 2.39±1.04abk_Bacteria;p_Acidobacteria 0.93±0.50 a 1.35±0.32 a 1.02±0.13 a 1.09±0.03 a 0.88±0.17 a 1.23±0.19ak_Bacteria;p_Actinobacteria 7.02±1.78 a 8.74±0.08 a 7.28±1.55 a 8.31±0.99a 7.41±1.18 a 8.66±1.13ak_Bacteria;p_Bacteroidetes 32.72±2.21 a 33.33±2.81 ab 37.32±3.60 ab 35.96±0.94ab 34.62±3.63 ab 38.39±2.09bk_Bacteria;p_Chlorobi 0.31±0.07 a 0.40±0.12 ab 0.45±0.05 b 0.29±0.05a 0.34±0.05 ab 0.32±0.04abk_Bacteria;p_Chloroflexi 1.61±0.24 a 2.95±0.36 b 3.01±1.34 b 2.26±0.32ab 2.28±0.28 ab 2.87±0.67abk_Bacteria;p_Firmicutes 2.15±0.38 a 2.98±0.38 ab 2.17±0.77 a 3.53±0.46b 2.54±0.75 ab 3.09±0.69abk_Bacteria;p_Gemmatimonadetes 0.46±0.08 a 0.64±0.08 a 0.68±0.21 a 0.56±0.09a 0.54±0.05 a 0.64±0.11ak_Bacteria;p_Nitrospirae 0.17±0.07 a 0.29±0.13 a 0.22±0.03 a 0.23±0.07a 0.31±0.02 a 0.32±0.18ak_Bacteria;p_Proteobacteria 23.57±1.60 a 30.46±0.95 b 27.30±4.68 ab 30.85±2.21b 23.57±2.17 a 28.04±1.61abk_Bacteria;p_Spirochaetes 0.12±0.03 a 0.30±0.10 b 0.20±0.05 ab 0.32±0.06b 0.30±0.05 b 0.28±0.11bk_Bacteria;p_TM7 24.34±5.26 c 13.85±4.08 ab 18.63±5.23 bc 11.03±0.99a 22.34±4.16 c 12.92±2.05abk_Bacteria;p_Verrucomicrobia 0.28±0.05 a 0.29±0.02 a 0.35±0.03 b 0.27±0.01a 0.43±0.05 c 0.38±0.04bcOthers 0.34±0.05 a 0.38±0.03 ab 0.41±0.02 ab 0.32±0.03a 0.36±0.03 ab 0.47±0.15b

1) Mean±SD, data contained a same letter in the same rows are not significantly different (P＞0.05).

Table S3 Relative abundance of bacterial order in rhizospheric soils of the two cultivars (%)

TaxonCK T1 T2

Cutai Sijiu Cutai Sijiu Cutai SijiuCytophagales 11.88±0.51 a1) 14.38±2.47ab 15.52±0.47b 13.11±1.42ab 13.18±1.83ab 16.06±1.38bSaprospirales 15.00±1.44 a 12.61±0.82a 15.45±1.75a 12.06±0.67a 14.37±3.08a 14.28±1.50aBurkholderiales 8.73±1.33ab 9.27±0.90ab 8.14±1.34a 10.88±1.13b 8.06±1.23a 9.33±0.92abActinomycetales 6.25±1.56a 7.30±0.31a 6.37±1.34a 7.17±0.91a 6.38±1.06a 7.24±1.12aXanthomonadales 4.22±0.21a 5.62±0.39a 5.49±1.40a 5.38±0.19a 4.36±0.69a 5.66±0.94aI025 5.23±1.62a 2.96±0.71a 5.76±0.68a 1.90±0.30a 4.64±1.98a 2.01±0.49aMethanosarcinales 5.57±2.51c 3.68±2.95ab 0.92±0.52a 4.67±1.94c 3.94±3.81bc 2.24±0.99aFlavobacteriales 2.50±1.33a 2.30±0.72a 2.40±1.49a 5.33±2.92a 2.30±0.62a 2.67±0.74aSphingobacteriales 2.17±0.52ab 2.07±0.15a 2.81±0.37a 2.63±0.10b 3.35±0.23c 2.82±0.41abRhizobiales 1.75±0.08a 2.06±0.09bc 2.22±0.21c 1.98±0.10ab 1.96±0.16ab 1.94±0.11abClostridiales 1.34±0.15a 2.09±0.32bc 1.22±0.48a 2.34±0.27c 1.70±0.60ab 2.26±0.60bcBacteroidales 1.09±0.27ab 1.90±0.19bc 1.05±0.33a 2.77±0.33c 1.35±0.78ab 2.50±0.13cRoseiflexales 0.84±0.12a 1.64±0.19bc 1.75±1.03d 1.21±0.19ab 1.36±0.17ab 1.73±0.50cdAlteromonadales 1.06±0.45a 1.40±0.67a 1.11±0.69a 1.18±0.41a 0.77±0.11a 1.14±0.05aPseudomonadales 0.75±0.23a 0.88±0.40a 1.18±1.01a 1.65±0.46a 0.97±0.11a 0.82±0.10aBacillales 0.75±0.24a 0.82±0.06a 0.89±0.27a 1.11±0.18a 0.79±0.16a 0.75±0.08aMyxococcales 0.57±0.13a 1.08±0.13ab 0.82±0.11ab 0.87±0.04b 0.64±0.05ab 0.75±0.10aNitrosomonadales 0.61±0.15a 0.80±0.04d 0.65±0.18bc 0.79±0.14c 0.79±0.08c 1.05±0.34eDesulfuromonadales 0.57±0.32a 1.23±0.41ab 0.63±0.02a 0.81±0.06ab 0.26±0.04ab 0.64±0.31bAcidimicrobiales 0.37±0.11ab 0.63±0.06c 0.43±0.08ab 0.51±0.05bc 0.45±0.04b 0.68±0.12cSolibacterales 0.36±0.18a 0.66±0.15bc 0.49±0.08a 0.52±0.03ab 0.37±0.03a 0.56±0.14cSphingomonadales 0.34±0.04a 0.43±0.05b 0.54±0.11ab 0.42±0.06ab 0.56±0.11a 0.47±0.03ab

SC-I-84 0.36±0.07a 0.46±0.13ab 0.42±0.05b 0.35±0.03ab 0.36±0.03b 0.48±0.06abAcidobacteriales 0.33±0.23a 0.42±0.11a 0.27±0.04a 0.34±0.01a 0.32±0.09a 0.34±0.07aRhodocyclales 0.18±0.05a 0.50±0.18a 0.27±0.09a 0.46±0.19a 0.22±0.08a 0.38±0.10aGallionellales 0.55±0.24b 0.54±0.35b 0.22±0.06ab 0.30±0.01ab 0.12±0.02a 0.18±0.03abPedosphaerales 0.26±0.05a 0.27±0.03a 0.34±0.03b 0.25±0.00a 0.40±0.05b 0.36±0.04bCaulobacterales 0.30±0.04ab 0.36±0.06b 0.25±0.03a 0.32±0.07ab 0.33±0.03ab 0.33±0.02abRhodospirillales 0.21±0.02a 0.36±0.01c 0.32±0.06bc 0.29±0.00abc 0.26±0.04ab 0.37±0.08cSpirobacillales 0.31±0.14a 0.32±0.11a 0.25±0.04a 0.27±0.04a 0.28±0.15a 0.25±0.10aEllin329 0.16±0.04a 0.27±0.05b 0.26±0.05b 0.28±0.02b 0.35±0.05b 0.32±0.07bNitrospirales 0.17±0.07a 0.29±0.13a 0.22±0.03a 0.23±0.07a 0.31±0.02a 0.32±0.18aGaiellales 0.15±0.05a 0.33±0.01c 0.21±0.08ab 0.24±0.02ab 0.17±0.04a 0.29±0.04bcEW055 0.30±0.11b 0.16±0.04a 0.33±0.09b 0.13±0.01a 0.30±0.07b 0.15±0.05aSolirubrobacterales 0.15±0.04a 0.29±0.08b 0.19±0.04a 0.22±0.01ab 0.19±0.02a 0.27±0.01bBdellovibrionales 0.19±0.06a 0.24±0.04a 0.22±0.04a 0.23±0.02a 0.23±0.01a 0.19±0.05aChloroflexales 0.11±0.01a 0.22±0.03ab 0.25±0.08b 0.21±0.09ab 0.17±0.02ab 0.27±0.10bSpirochaetales 0.09±0.03a 0.20±0.06b 0.15±0.04ab 0.24±0.06b 0.27±0.07b 0.25±0.09b

1) Mean±SD, data contained a same letter in the same rows are not significantly different (P＞0.05).

Table S4 Relative abundance of bacterial genus in rhizospheric soils of the two cultivars (%)

TaxonCK T1 T2

Cutai Sijiu Cutai Sijiu Cutai SijiuFlavisolibacter 8.83±1.18b1) 5.86±0.84a 7.53±1.08ab 5.58±0.42a 5.71±1.70a 5.94±1.41aMethanosarcina 5.55±2.50a 3.67±2.95a 0.91±0.53a 4.63±1.92a 3.92±3.78a 2.24±1.00aFlavobacterium 1.81±1.29a 1.65±0.69a 1.11±0.59a 4.15±2.16b 1.56±0.43a 1.72±0.79aStreptomyces 1.75±0.75a 1.77±0.35a 1.29±0.36a 1.41±0.23a 1.57±0.60a 1.85±0.45aPseudoxanthomonas 1.22±0.17a 1.12±0.31a 1.56±0.74a 1.17±0.03a 1.14±0.19a 1.33±0.17aGeobacter 0.55±0.31ab 1.20±0.42c 0.61±0.02ab 0.78±0.06bc 0.25±0.04a 0.62±0.30abClostridium 0.45±0.05ab 0.78±0.10c 0.30±0.10a 0.86±0.15c 0.61±0.20bc 0.83±0.26cDevosia 0.67±0.12ab 0.63±0.06ab 0.70±0.03b 0.59±0.01ab 0.68±0.09ab 0.53±0.10aGeorgenia 0.46±0.12ab 0.87±0.30b 0.42±0.16a 0.82±0.22ab 0.42±0.18a 0.70±0.29abAdhaeribacter 0.72±0.42a 0.42±0.05a 0.83±0.13a 0.45±0.07a 0.64±0.35a 0.45±0.18aPseudomonas 0.52±0.24a 0.39±0.09a 0.82±0.75a 0.78±0.21a 0.40±0.07a 0.37±0.07aSteroidobacter 0.43±0.02a 0.65±0.05b 0.45±0.14a 0.45±0.04a 0.47±0.10a 0.53±0.06abFluviicola 0.34±0.04a 0.35±0.10a 0.56±0.30a 0.37±0.09a 0.34±0.10a 0.61±0.10aCellvibrio 0.40±0.21a 0.42±0.15a 0.50±0.38a 0.51±0.36a 0.27±0.07a 0.36±0.07aGallionella 0.55±0.24b 0.54±0.35b 0.22±0.06ab 0.30±0.01ab 0.12±0.02a 0.18±0.03aDokdonella 0.30±0.06a 0.28±0.03a 0.30±0.15a 0.34±0.04a 0.25±0.00a 0.40±0.07aAcinetobacter 0.07±0.01a 0.23±0.20ab 0.16±0.10ab 0.50±0.11c 0.38±0.11bc 0.34±0.19bcBacillus 0.28±0.07a 0.24±0.03a 0.20±0.03a 0.45±0.08b 0.28±0.07a 0.23±0.05aCellulomonas 0.21±0.03ab 0.37±0.14b 0.20±0.04a 0.34±0.10ab 0.19±0.06a 0.33±0.08abSporocytophaga 0.38±0.29a 0.25±0.02a 0.21±0.05a 0.24±0.05a 0.23±0.07a 0.28±0.10aKaistobacter 0.20±0.02a 0.22±0.06ab 0.31±0.05bc 0.22±0.05ab 0.35±0.07c 0.25±0.05abcTerracoccus 0.28±0.07a 0.24±0.02a 0.22±0.06a 0.28±0.11a 0.22±0.05a 0.24±0.06a

Rhodoplanes 0.18±0.04a 0.25±0.04a 0.27±0.05a 0.25±0.04a 0.22±0.01a 0.26±0.08aCandidatus Solibacter 0.19±0.09a 0.33±0.09b 0.25±0.06ab 0.25±0.01ab 0.18±0.02a 0.22±0.05abNitrospira 0.16±0.07a 0.27±0.13a 0.20±0.03a 0.21±0.08a 0.29±0.02a 0.30±0.18aLimnobacter 0.15±0.06a 0.22±0.03a 0.14±0.05a 0.33±0.12a 0.14±0.04a 0.30±0.23aBdellovibrio 0.18±0.04a 0.24±0.04a 0.21±0.04a 0.21±0.02a 0.22±0.01a 0.18±0.06aCrocinitomix 0.15±0.07a 0.13±0.03a 0.20±0.04a 0.27±0.07a 0.25±0.16a 0.24±0.05aAgrobacterium 0.21±0.01a 0.19±0.02a 0.21±0.03a 0.19±0.03a 0.22±0.05a 0.18±0.05aChryseobacterium 0.10±0.01a 0.04±0.02a 0.47±0.71a 0.45±0.65a 0.09±0.03a 0.04±0.01aMethylibium 0.06±0.03a 0.16±0.02ab 0.30±0.23b 0.20±0.05ab 0.18±0.02ab 0.25±0.13abSpirochaeta 0.08±0.03a 0.19±0.05b 0.12±0.03ab 0.20±0.05b 0.22±0.06b 0.21±0.08bActinotalea 0.13±0.03a 0.15±0.03a 0.16±0.03a 0.20±0.06a 0.20±0.11a 0.18±0.05aCoprococcus 0.13±0.03a 0.15±0.04a 0.08±0.02a 0.28±0.04b 0.16±0.09a 0.17±0.04aPontibacter 0.25±0.24a 0.11±0.04a 0.22±0.05a 0.11±0.01a 0.18±0.10a 0.11±0.07aCytophaga 0.17±0.07ab 0.14±0.06ab 0.17±0.05ab 0.26±0.12b 0.11±0.01a 0.13±0.03aActinomadura 0.11±0.04a 0.14±0.03a 0.31±0.08b 0.12±0.02a 0.10±0.05a 0.16±0.06aPhenylobacterium 0.16±0.02ab 0.20±0.03b 0.12±0.02a 0.16±0.03ab 0.13±0.02a 0.16±0.02abSaccharopolyspora 0.10±0.04a 0.19±0.07ab 0.28±0.12b 0.11±0.00a 0.09±0.03a 0.14±0.03aCandidatus Koribacter 0.14±0.10a 0.18±0.05a 0.12±0.03a 0.15±0.03a 0.12±0.02a 0.16±0.04a

1) Mean±SD, data contained a same letter in the same rows are not significantly different (P＞0.05).

Table S5 Relative abundance of fungal phylum in rhizospheric soils of the two cultivars (%)

TaxonCK T1 T2

Cutai Sijiu Cutai Sijiu Cutai Sijiuk__Fungi;p__Ascomycota 19.03±10.23 a1) 16.83±2.89 a 15.77 ±2.45

a22.78±1.36 a 26.67±15.15 a 18.08±6.29 a

k__Fungi;p__Basidiomycota 1.88±1.25a 10.45±7.91 ab 3.07±0.85 a 21.95±9.05 bc 17.61±6.64 bc 30.25±11.12 ck__Fungi;p__Chytridiomycota 3.08±1.66ab 5.24±2.52 b 4.99±1.93 b 0.56±0.39 a 0.80±0.57 a 1.34±0.60 ak__Fungi;p__unidentified 75.80±7.89b 67.22±10.40 ab 75.46±2.32 b 54.27±7.40 a 54.71±13.89 a 50.00±9.57 a

1) Mean±SD, data contained a same letter in the same rows are not significantly different (P＞0.05).

Table S6 Relative abundance of fungal order in rhizospheric soils of the two cultivars (%)

TaxonCK T1 T2

Cutai Sijiu Cutai Sijiu Cutai SijiuAgaricales 1.07±0.71 a1) 0.83±0.23 a 1.51±0.90 a 3.73±2.9 b 2.37±1.15 ab 9.25±11.14 bHypocreales 0.47±0.52 a 0.23±0.09 a 0.58±0.41 a 0.74±0.51 a 0.26±0.03 a 0.22±0.10 aPezizales 12.04±8.50 b 5.91±2.12 a 6.08±2.15 a 7.93±0.35 a 14.11±15.32 b 4.84±1.57 aPleosporales 0.27±0.07 a 0.10±0.02 a 0.23±0.17 a 0.13±0.01 a 0.83±1.01 a 0.12±0.05 aRhizophlyctidales 3.01±1.62 ab 5.14±2.62 b 4.96±1.93 b 0.46±0.31 a 0.78±0.56 a 1.30±0.59 a

Sordariales 5.64±2.21 a9.62±1.47 ab 7.82±0.39 ab 12.06±1.13 b 9.69±2.82 ab 11.75±3.97 b

Tremellales 0.06±0.05 a 0.06±0.03 a 0.26±0.14 b 0.10±0.06 ab 0.10±0.09 ab 0.12±0.12 ab

Trichosporonales 0.72±0.56 a 9.52±8.11 ab 1.25±0.27 a17.96±11.62 b 14.96±5.74 b 20.83±2.98 b

Xylariales 0.05±0.05 a 0.01±0.01 a 0.01±0.00 a 0.02±0.01 a 0.52±0.88 a 0.05±0.08 a

1) Mean±SD, data contained a same letter in the same rows are not significantly different (P＞0.05).

Table S7 Relative abundance of fungal genus in rhizospheric soils of the two cultivars (%)

TaxonCK T1 T2

Cutai Sijiu Cutai Sijiu Cutai SijiuAscobolus 6.90±3.40 b1) 3.11±1.94 a 1.90±0.73 a 4.87±0.48 ab 2.50±0.82 a 2.18±0.53 aBullera 0.05±0.05 a 0.05±0.02 a 0.22±0.11 b 0.09±0.06 ab 0.08±0.10 ab 0.10±0.12 abCiliophora 0.11±0.04 a 0.24±0.14 a 0.07±0.03 a 0.15±0.07 a 0.59±0.86 a 0.13±0.03 aFusarium 0.42±0.51 a 0.11±0.04 a 0.45±0.39 a 0.47±0.54 a 0.14±0.02 a 0.13±0.11 aMonographella 0.05±0.05 a 0.01±0.01 a 0.01±0.01 a 0.02±0.01 a 0.52±0.88 a 0.01±0.01 aPeyronellaea 0.13± 0.03 a 0.00±0.00 a 0.11±0.17 a 0.03±.010 a 0.70±1.03 a 0.01±0.01 aRhizophlyctis 3.01±1.62 ab 5.14±2.62 b 4.96±1.93 b 0.46±0.31 a 0.78±0.56 a 1.30±0.59 aTrichocladium 2.74±0.18 a 4.05±0.68 ab 4.13±1.02 ab 8.60±1.15 c 4.05±0.54 ab 5.35±1.98 bTrichosporon

0.72±0.56 a 9.52±8.11 ab 1.25±0.27 ab 17.96±11.62 b14.96±5.74 b 20.83±2.98 b

Zopfiella 0.08±0.04 a 0.97±0.60 b 0.36±0.23 ab 0.53±0.34 ab 0.50±0.33 ab 0.51±0.28 ab

1) Mean±SD, data contained a same letter in the same rows are not significantly different (P＞0.05).

Table S8 Rotated component matrix for the microbial carbon sources utilizations in the Biolog EcoPlate assay

CK T1 T2Cutai Sijiu Cutai Sijiu Cutai Sijiu

Substrate PC1 PC2 Substrate PC1 PC2 Substrate PC1 PC2 Substrate PC1 PC2Substrat

ePC1 PC2 Substrate PC1 PC2

D1 1.000 0.028 D2 0.998 -0.061 H3 -

1.000

-0.021 F4 0.999 0.041 F1 1.000 0.019 D2 0.992 0.124

B2 0.999 0.048 E4 -

0.997

-0.083 F2 1.000 -0.023 H3 0.997 0.081 H4 -0.994 0.108 D4 0.983 0.183

A2 -

0.987

-0.163 H1 0.996 0.091 C3 0.999 -0.032 G4 -

0.993

0.117 G2 0.994 -0.114 C3 0.976 -0.220

G4 0.985 0.171 E2 0.988 0.155 C1 0.998 0.070 E2 0.992 -0.123 H1 -0.987 -

0.163

B4 0.946 0.323

B3 0.982 -0.190 B1 0.986 -0.167 A3 -

0.995

0.095 B3 0.987 0.160 H3 -0.982 -

0.187

G4 0.938 0.347

C2 0.971 0.238 G3 0.976 -0.220 H2 -

0.994

0.108 H2 0.985 0.171 B2 0.974 -

0.225

H2 0.933 -0.359

E4 -

0.966

0.259 F2 0.957 0.290 E3 -

0.993

0.119 C4 0.982 -0.190 G4 -0.973 0.232 E2 0.929 0.371

E2 -

0.954

0.301 D3 0.956 0.292 G4 0.983 0.183 F3 0.979 -0.202 B1 0.969 0.247 G1 0.921 0.389

E3 0.945 0.327 D1 0.943 0.334 F3 -

0.979

-0.205 A3 0.972 -0.236 E3 0.966 0.258 C4 -0.910 0.415

A4 0.923 -0.386 G4 -

0.922

0.388 A4 0.943 0.333 E3 0.964 -0.267 F4 -0.894 0.449 E3 0.905 0.425

C4 0.873 0.488 E1 0.918 0.397 C4 -

0.936

-0.351 G2 0.950 0.312 C3 -0.873 0.488 G2 0.902 0.432

H2 0.869 -0.494 B2 -

0.909

0.417 F4 -

0.922

0.388 A4 0.948 0.319 E2 -0.862 -

0.506

A3 0.900 -0.435

C3 0.867 0.499 H4 0.899 0.437 B1 0.893 -0.450 G1 0.935 0.356 C4 0.859 -

0.512

G3 0.884 -0.468

H4 0.864 0.504 C1 0.844 0.536 G3 -

0.848

0.530 D3 0.932 -0.362 G1 -0.844 0.537 A2 0.864 -0.504

F3 0.825 -0.566 A3 -

0.839

-0.544 D4 -

0.822

0.570 F1 -

0.917

0.398 B3 0.787 0.617 D3 -0.814 0.581

B1 -

0.814

-0.581 B4 -

0.760

0.650 E4 0.821 -0.571 G3 0.896 -0.445 H2 -0.783 0.622 E4 0.807 0.590

C1 0.809 -0.588 C4 0.718 -0.696 H4 0.807 -0.591 B1 0.895 -0.446 A3 0.749 0.662 H4 0.805 -0.593

D4 0.726 0.688 B3 -

0.035

0.999 G2 0.749 0.663 D2 0.838 0.546 D4 0.049 0.999 D1 0.779 -0.627

F4 0.066 -0.998 C2 0.050 0.999 C2 0.730 -0.684 C2 0.732 0.681 A2 0.167 0.986 C1 0.736 0.677

H1 0.107 0.994 G2 -

0.137

-0.991 D2 -

0.727

-0.687 H4 -

0.731

-0.682 E1 -0.216 0.976 E1 -0.052 0.999

D2 0.256 -0.967 C3 -

0.388

0.922 D3 0.720 -0.694 B4 0.071 0.997 C2 0.278 -

0.960

F1 -0.085 -0.996

D3 -

0.334

0.942 H2 0.402 0.916 B4 0.086 -0.996 E1 -

0.091

0.996 G3 -0.349 -

0.937

B2 -0.187 -0.982

F2 -

0.342

0.940 A4 -

0.415

-0.910 B3 0.301 0.954 C3 0.193 -0.981 D2 -0.436 0.900 F4 0.192 0.981

H3 0.380 -0.925 F3 0.424 0.906 A2 0.463 0.886 E4 0.446 0.895 D1 0.463 -

0.886

C2 0.360 0.933

G1 0.502 0.865 D4 0.442 0.897 H1 0.469 0.883 H1 0.505 0.863 A4 0.488 0.873 H1 0.435 0.900

F1 0.526 0.850 F4 0.461 0.887 B2 -

0.470

-0.883 D1 0.506 -0.862 B4 0.491 0.871 B1 0.443 -0.897

G3 0.528 -0.849 G1 0.566 -0.825 D1 -

0.559

-0.829 F2 -

0.507

0.862 F3 0.553 -

0.833

F3 0.496 0.868

E1 -

0.594

-0.804 A2 -

0.588

0.809 F1 0.601 0.799 C1 -

0.569

0.822 E4 -0.563 -

0.827

A4 0.570 -0.822

G2 -

0.597

0.802 H3 -

0.622

0.783 E1 0.644 0.765 A2 -

0.605

-0.797 D3 0.609 0.793 F2 0.614 -0.789

A3 0.605 -0.796 E3 0.649 -0.760 G1 -

0.645

0.764 B2 0.616 -0.788 C1 -0.688 0.726 B3 0.634 -0.773

B4 0.615 0.788 F1 0.698 -0.716 E2 -

0.668

0.744 D4 -

0.649

-0.761 F2 0.704 0.710 H3 0.676 0.737

The details on the substrate designation are given in Table S1.

Table S9 Mean AWCD of each carbon source in the Biolog EcoPlate assay

SubstrateCK T1 T2

Cutai Sijiu Cutai Sijiu Cutai SijiuB1 2.34±0.07b1) 1.75±0.29ab 2.36±0.25b 2.04±0.46ab 1.65±0.43a 1.42±0.48aC1 2.52±0.05ab 2.60±0.17bc 3.13±0.07d 2.30±0.23a 2.47±0.04ab 2.84±0.18cD1 2.20±0.08a 2.41±0.30a 2.46±0.12a 2.25±0.07a 2.32±0.08a 2.74±0.15bE1 2.28±0.26cd 2.11±0.39bc 1.73±0.16ab 2.65±0.03d 2.06±0.38bc 1.53±0.18aF1 2.51±0.07b 2.39±0.28b 2.37±0.13b 2.34±0.33b 2.43±0.09b 1.41±0.23aG1 2.18±0.09a 1.98±0.16a 1.98±0.19a 2.12±0.06a 1.97±0.05a 2.21±0.26aH1 1.94±0.17a 1.83±0.21a 2.24±0.34a 2.31±0.28a 2.01±0.26a 2.21±0.26aA2 1.65±0.02bc 1.43±0.20ab 1.80±0.10c 1.83±0.04c 1.51±0.24bc 1.14±0.27aB2 2.39±0.07c 0.80±0.28a 2.23±0.06c 1.42±0.29b 1.45±0.52b 1.22±0.07abC2 2.29±0.02c 1.36±0.22b 1.22±0.36ab 2.30±0.08c 1.18±0.07ab 1.00±0.05aD2 2.63±0.08ab 2.56±0.13ab 2.52±0.18ab 2.77±0.03ab 2.79±0.17b 2.51±0.19aE2 2.10±0.08a 1.93±0.08a 2.01±0.07a 2.18±0.05a 2.08±0.08a 2.07±0.36aF2 2.14±0.12c 1.70±0.22b 2.21±0.06c 1.67±0.06b 1.45±0.21ab 1.35±0.11aG2 1.47±0.20cd 1.18±0.19ab 1.61±0.12d 0.95±0.02a 1.22±0.07bc 0.93±0.15aH2 0.35±0.04d 0.19±0.06b 0.32±0.05cd 0.25±0.06bc 0.39±0.06d 0.07±0.05aA3 1.61±0.04d 0.89±0.06a 1.54±0.06d 1.48±0.12cd 1.25±0.12bc 1.02±0.30abB3 1.78±0.03bc 1.78±0.16bc 1.99±0.12c 1.83±0.14bc 1.44±0.12a 1.68±0.14bC3 0.30±0.27a 0.17±0.05a 0.24±0.13a 0.26±0.30a 0.07±0.08a 0.08±0.06aD3 2.23±0.08b 2.35±0.26bc 2.45±0.10bc 2.61±0.12c 2.12±0.15ab 1.88±0.25aE3 1.19±0.08b 0.24±0.10a 1.25±0.12b 0.26±0.05a 0.60±0.50a 0.29±0.11aF3 1.67±0.12c 1.25±0.11a 1.73±0.04c 1.40±0.10ab 1.52±0.10bc 2.10±0.19dG3 0.41±0.03a 0.56±0.19ab 0.63±0.18ab 0.75±0.11b 0.45±0.04a 0.42±0.10aH3 1.80±0.08ab 1.20±0.71a 1.99±0.20b 1.99±0.08b 1.51±0.13ab 1.41±0.30abA4 2.04±0.12bc 1.97±0.25b 2.37±0.20c 2.03±0.24bc 1.38±0.22a 2.29±0.17bcB4 2.22±0.13b 1.96±0.06a 2.05±0.04a 1.98±0.04a 2.27±0.08b 2.06±0.09aC4 2.04±0.24bc 1.24±0.23a 2.04±0.18bc 2.36±0.36c 1.65±0.22ab 1.43±0.21aD4 2.18±0.08b 2.16±0.11b 1.94±0.08a 1.78±0.03a 1.89±0.06a 2.19±0.13bE4 1.26±0.07b 0.98±0.10b 1.19±0.34b 1.28±0.33b 1.06±0.06b 0.41±0.13aF4 1.62±0.10b 1.73±0.32b 1.16±0.25a 2.20±0.17c 1.71±0.05b 1.26±0.11aG4 2.16±0.15b 2.47±0.02bc 2.39±0.20bc 2.63±0.05c 1.68±0.29a 2.22±0.29bH4 0.67±0.04b 0.80±0.02c 0.79±0.08c 0.86±0.01c 0.55±0.08a 0.75±0.11bc

1)Mean±SD, data contained a same letter in the rows are not significantly different (P>0.05)