arun curriculum vitae_2015
TRANSCRIPT
Arun Kumar Singh PhD Born: 27 Oct, 1980Department of Biotechnology Nationality: IndianPune University, India Skype: arun.singh614Email: [email protected] Address: 65, New Basti Raidopur,
Behind Suraj Hall,Azamgarh 276001 UP India.
Mobile no: 91-9974339692, 91-8527974946
Education:PhD: Department of Biotechnology, Pune University, India
Specialization: BiotechnologyMSc Biotechnology, MSU Baroda, Vadodara, IndiaBSc Chemistry, Banaras Hindu University, Varanasi India.
Professional experience: During my PhD, I have studied ubiquitin signalling in innate immunity. I have submitted
my PhD thesis to Pune University entitled “Study the interaction of Staphylococcus aureus with Ubiquitin-Proteasome system” where I have studied the role of ubiquitin in sensing and subsequent lysosomal degradation of S.aureus in the mammalian cytosol. I also identified an ubiquitin interacting protein from S.aureus and studied its role in pathogenesis of S.aureus.
I joined ICGEB New Delhi as Research Associate and worked on cloning and expression of Dengue 2 envelop proteins using Baculovirus expression system at Joint ICGEB-Emory Vaccine Center.
Awards, Honors and Fellowship:1. Fellowship for MSc by Department of Biotechnology.2. Graduate Aptitude Test (GATE) conducted by IITs and IISc, India.3. National Eligibility Test (NET-LS) conducted by CSIR India.4. Senior Research Fellowship awarded by ICMR India.5. Best poster award at 2nd Annual Conference of Society for Mitochondrial Research and Medicine-India
Professional skill: My technical expertise includes cloning and overexpression of proteins in E.coli and their purification by affinity chromatography. I have also done protein expression in Baculovirus expression system. Enzymatic assays, western blotting, confocal and fluorescence microscopy, DNA and RNA isolation, PCR, transfection and handling of mammalian and insect cell lines. S.aureus genetic manipulation: generated gene (LAP) specific knockout of S.aureus and studied the role of gene, ELISA and viral plaque assay.
Publications:
Original research articles:
1. Singh AK, Singh R, Tomar D, Pandya CD, Singh R. The leucine aminopeptidase of Staphylococcus aureus is secreted and contributes to biofilm formation. International
journal of infectious diseases 2012; 16(5):e375-81.
2. Tomar D, Singh R, Singh AK, Pandya CD, Singh R. TRIM13 regulates ER stress induced autophagy and clonogenic ability of the cells. Biochimica et biophysica acta: Mol Cell Research 2012; 1823(2): 316-26.
3. Sripada L, Tomar D, Prajapati P, Singh R, Singh AK, Singh R. Systematic Analysis of Small RNAs Associated with Human Mitochondria by Deep Sequencing: Detailed Analysis of Mitochondrial Associated miRNA. PloS one 2012; 7(9): e44873
4. Tomar D, Sripada L, Prajapati P, Singh R, Singh AK., Singh R. Nucleo-Cytoplasmic Trafficking of TRIM8, a Novel Oncogene, Is Involved in Positive Regulation of TNF Induced NF-kappaB Pathway. PloS one 2012; 7(11): e48662.
5. Tomar D, Prajapati P, Sripada L, Singh K, Singh R, Singh AK., Singh R. TRIM13 regulates translocation of caspase-8 to autophagosomes, its activation, and cell death during ER stress. Biochimica et biophysica acta: Mol Cell Research, manuscript accepted for publication.
6. Neha Rajpara, Kittappa Vinothkumar, Priyabrata Mohanty, Arun Kumar Singh, Rajesh Singh, Ritam Sinha, Dhrubajyoti Nag, Hemanta Koley, Ashima Kushwaha Bhardwaj Synergistic Effect of Various Virulence Factors Leading to High Toxicity of Environmental V. cholerae Non-O1/ Non-O139 Isolates Lacking ctx Gen :Comparative Study with Clinical Strains23 Sep 2013 | PLOS ONE10.1371/journal.pone.0076
7. Singh K, Poteryakhina A, Zheltukhin A, Bhatelia K, Prajapati P, Sripada L, Tomar D, Singh R, Singh AK, Chumakov PM, Singh R. NLRX1 acts as tumor suppressor by regulating TNF-α induced apoptosis and metabolism in cancer cells.Biochim Biophys Acta. 2015 Jan 30;1853(5):1073-1086. doi: 10.1016/j.bbamcr.2015.01.016. [Epub ahead of print]PMID:25639646
Manuscript in communication:
Arun Kumar Singh , Pooja Patel, Dhanendra Tomar , Rochika Singh , Lakshmi Sripada , Paresh Prajapati , Kritarth Singh , Rajesh Singh. P62/SQSTM1 mediated autophagic clearance of intracellular Staphylococcus aureus in human epithelial cells.
Poster presentations at International conferences:
1. Singh AK, Patel P, Sripada L, Singh R, Singh K, Tomar D, Singh R
S.aureus infection enhances mitochondrial biogenesis in human epithelial cells.
3rd Annual Conference of Society for Mitochondrial Research and Medicine-India Mitochondria in Health and Disease”.19th -20th December 2013. NIMHANS Bangalore India
2. Singh AK, Patel P, Tomar D, Singh R, Singh K, Roy M, Prajapati P, Singh R.
Leucyl aminopeptidase, Ubiquitin binding protein, inhibits NF-kB activity during S.aureus infection in human epithelial cells. International Symposium on “Conceptual Advances in Cellular Homeostasis Regulated by Proteases and Chaperones”. The Advanced Centre for Treatment, Research and Education in Cancer (ACTREC) Mumbai India Dec. 3-6, 2013.
3. Singh AK, Patel P, Singh R, Prajapati P, Tomar D, Sripada L, Singh K, Singh R.
Mitochondrial ROS negatively regulates intracellular survival of S. aureus in human epithelial cells. 2nd Annual Conference of Society for Mitochondrial Research and Medicine-India “Mitochondria in Health and Disease”. Central University of Gujarat, Gandhinagar, India, November 2-3, 2012. Best poster award.
4. Tomar D, Singh R, Singh AK, Singh R. TRIM13 regulates autophagosome biogenesis on ER membrane and suppresses clonogenic ability of cells via autophagy. The XXXV All India Cell Biology conference and symposium on Membrane dynamics and disease, National Institute of Science Education and Research (NISER), Bhubaneswar, Orissa, India, December 16-18, 2011.
5. Singh AK, Singh R, Tomar D, Pandya C, Singh R. Identification and biochemical characterization of Leucyl aminopeptidase from Staphylococcus aureus. International Conference on Understanding and Managing Pathogenic Microbes, Institute of Microbial Technology, Chandigarh, India, January 22-24, 2010.
6. Tomar D, Singh AK, Singh R. Conjugation of endogenous Escherichia coli and
Staphylococcus aureus proteins through human small ubiquitin like modifier (SUMO) protein. International Conference on Emerging Trends in Biotechnology, Banaras Hindu University, Varanasi, India, December 04-06, 2009.
References:
1. Dr. Rajesh Singh,(Ph.D. Mentor) [email protected] Professor Department of Biochemistry MS University of Baroda, Vadodara 390002 Gujarat India
2. Dr Anmol [email protected] ProfessorJoint ICGEB-Emory Vaccine CenterInternational Center for Genetic Engineering and Biotechnology,Aruna Asaf Ali Marg,Vasant Kunj, New Delhi – 110067
3. Dr. Sunil Singh [email protected] ProfessorDepartment of BotanyMS University of Baroda, Vadodara390002 Gujarat India
4. Dr. Ashima K [email protected] Professor and Group Leader Department of Human Health and Diseases, School of Biological Sciences and Biotechnology Indian Institute of Advanced Research, Koba Institutional Area Gandhinagar, Gujarat- 382007, India