as biological inoculants for improved plant growth klára ... · as biological inoculants for...
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Klára BradáčováPhD. Student
University Hohenheim, Germany
Microbial consortia productsas biological inoculants for improved plant growth
113 - 14 DEC 2017 PPHE Braunschweig- Klára Bradáčová
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Microbial Consortia Products (MCP)
2
Nutrient mobilizationRoot exudates
Enzyme secretionActivation of C, N, P turnover enzymes
Siderophores
Growth promotionBiotic and abiotic stress
toleranceVigorous, strong, healthy plants
Yield increase
Beneficial microbes
Plant response
(Modified from Schenk et al., Trend in Biotech., 2011)
13 - 14 DEC 2017 PPHE Braunschweig- Klára Bradáčová
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Introduction of Microbial ConsortiaProduct (MCP)
• description: dark brown liquid, should provide nutrient acquisition and enhancement of BNF
- bacterial composition: Azotobacter vinlandii, Clostridium sp., Lactobacillussp., Bacillus amyloliquefaciens, B. subtilis (SILoSil® BS), B. thuringiensis, Pseudomonas fluorescens, Acetobacter, Enterococcus, Rhizobiumjaponicum
- funghal composition: Saccharomyces, Penicillium roqueforti, Monascus, Aspergillus oryzae, Trichoderma harzianum (TRICHOSIL)
- plant composition: Arthrospira platensis (Spirulina), Ascophyllum nodosum
313 - 14 DEC 2017PPHE Braunschweig- Klára Bradáčová
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Principal approachesfor practical applications
Targeted SelectionHypothesis(Concept of BIOFECTOR)
Beneficial effects on plants achievedby targed selection and inoculationwith BEs particularly efficient forspecificapplications (e.g. nutrient mobilisation, root growth promotion, pathogen antagonism)
Auto-SelectionHypothesis(MCP Concept, EM Concept)
Inoculation with huge consortia ofplant growth-promoting bacteria withdifferent functions. Different stress conditionswill activate the most suitablepopulations(also targeted pre-activation ispossible prior to inoculation)
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413 - 14 DEC 2017 PPHE Braunschweig- Klára Bradáčová
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- Pot experiment (3 kg substrate: 1:3 sand/ soil mix) Maize cv. Jessy, 6 weeks)
- Soil: - Horb field site pH 6.5, silty loam, CAL-P 52, Nmin 11.3 (mg/kg soil)- - Practical course, stored soil, pH 5.6, silty loam, CAL-P 18
- Fertilisation levels (mg/kg soil):• 1.(N140, P130, K150, Mg50) Standard full nutrient supply
• 2. Reduced N/P fertilisation (N 70,P 50) or StdN and reduced P supply
- Different N forms: Nitrate N vs Ammonium N (ENTEC-stabilised Ammoniumsulfate, Novatec Solub, Compo)
- BE Treatments: ECAG 2895 (3 weekly fertigations, dosage according to manufacturer´srecommendations)
Maize pot experiments
- General measurements:plant growth (height, stem diameter), shootand root DM, root morphology (length diameter, fine roots) plant nutritional status
- Specific measurements:Functional charcterisation ofrhizosphere-microbial activities:(Enzyme assays in rhizosphere soilTracing for microorganisms, Auxin production potential) 513 - 14 DEC 2017 PPHE Braunschweig- Klára Bradáčová
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613 - 14 DEC 2017
0-Ctrl Nitrate fertilisation Ammonium fertilisation0
20406080
100120140160180
no BE BE
IAA production [relative values mg microbial biomass-1]
Nitrate fertilisation Ammonium fertilisation
a
b
b
bb
0-Ctrl NO3- NH4+
Rhizosphere acid phosphatase activity[nmol* g soil-1 h-1]
PPHE Braunschweig- Klára Bradáčová
BE
NH
4
Ctr
lNH
4
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PPHE Braunschweig- Klára Bradáčová 713 - 14 DEC 2017
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PPHE Braunschweig- Klára Bradáčová 813 - 14 DEC 2017
::Observed scenario Plant growth promotionBetter nutrient supply
Expected scenario
Improved spatialP acquisition Rhizosphere acidification
P solubilization
H+H+H+
Higher root colonization by bacteria
Increased production of IAA
Root elongationStimulation of root growth
Increased C, N, P turnoverin rhizosphere
Improved nutrient availability
Modified from Richardson, 2011
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Klára BradáčováUniversity Hohenheim
Germany
13 - 14 DEC 2017 PPHE Braunschweig- Klára Bradáčová 9
Test plant: maize (Zea mays cv. Jessy) grown on a clay loam pH 6.5,50 mg CAL-P/kg soil, 6 weeks cultivation
Bio-effector: ECAG2895 (liquid mixture of 16 aerobic and anaerobic bacteria,fungi, algae extracts) EuroChem Agro, Mannheim, Germany.
Fertilization levels (mg/kg soil): 1). N:140, P:80, K:150, Mg:50 = Standard full nutrient supply, 2). Reduced NP
fertilization (N:70, P:0), 3.) Reduced P fertilization (N:140, P:0), 4. Unfertiilzed Ctrl Different N forms: Nitrate vs stabilised ammonium sulfate (NovatecSolub)
METHODS - Maize pot experiments
monium supply, associated with improved P uptake and stimulation of root growth,
s produc- n. Effects
input.
roducts as biological inoculants ved growth of maize
mbinationin C/N/P
n in maizeon
n via stimulation of root growth (increased microbial auxin production) in combinationced promotion of enzymatic nutrient turn-over in the rhizosphere.
, insteadstrategyctions in
NH4+ fertilization increased the auxin production potentialof rhizosphere bacteria in the ECAG2895 variants.
sed total s.
Markus Weinmann1, Günter Neumann1, Uwe Ludewig1,n Kandeler1, Nils Berger2
f Soil Science, University Hohenheim, 70593 Stuttgart, Germany nzler-Müller Straße 23, 68165 Mannheim, Germany
Model for PGPM interactions in the rhizosphere
Lower rhizosphere acid phosphatase activity in ECAG2895variants as compared with the unfertilized control.No significant differences for alkaline phosphatase, glucosidase, xylosidase, cellulase and peptidase.
No BE BE
Root length [cm]
P content in shoot tissue [mg P plant -1]
NO3- fertilisation NH4+ fertilisation0-Ctrl
0-Ctrl NO3- NH4+
::Observed scenario Plant growth promotionBetter nutrient supply
Expected scenario
Improved spatialP acquisition Rhizosphere acidification
P solubilization
H+H+H+
Higher root colonization by bacteria
Increased production of IAA
Root elongationStimulation of root growth
Increased C, N, P turnoverin rhizosphere
Improved nutrient availability
Modified from Richardson, 2011
020406080
100120140160180
no BE BE
IAA production [relative values mg microbial biomass-1]
Nitrate fertilisation Ammonium fertilisation
a
b
b
bb
NO3- NH4+0-Ctrl
Rhizosphere- bacterial auxin production potential IAA Production mg-1 bacterial biomass
[rel. values]
roducts as biological inoculants ved growth of maize
Markus Weinmann1, Günter Neumann1, Uwe Ludewig1,n Kandeler1, Nils Berger2
f Soil Science, University Hohenheim, 70593 Stuttgart, Germany nzler-Müller Straße 23, 68165 Mannheim, Germany
INTRODUCTION
Test plant: maize (Zea mays cv. Jessy) grown on a clay loam pH 6.5, 50 mg CAL-P/kg soil, 6 weeks cultivation
Bio-effector: ECAG2895 (liquid mixture of 16 aerobic and anaerobic bacteria, fungi, algae extracts) EuroChem Agro, Mannheim, Germany.
Fertilization levels (mg/kg soil):
1). N:140, P:80, K:150, Mg:50 = Standard full nutrient supply, 2). Reduced NP fertilization (N:70, P:0), 3.) Reduced P fertilization (N:140, P:0), 4. Unfertiilzed Ctrl
Different N forms: Nitrate vs stabilised ammonium sulfate (NovatecSolub)
METHODS - Maize pot experiments
ECAG2695 shows positive effects on plant growth under ammonium supply, associated with improved P uptake and stimulation of root growth, particularly under reduced P availability.
ECAG2895 shows significant stimulation of shoot biomass produc-tion in combination with stabilised ammonium fertilization. Effects detectable with standard fertilization an with reduced N/P input.
CONCLUSIONS
AIM:Testing the effects of the microbial combination product ECAG2895 containing PGPMs involved in C/N/P turnover on early growth and nutrient acquisition in maize supplied with different levels of N and P fertilisation
RESULTS - Plant development
ECAG2695 stimulated plant growth by improved P acquisition via stimulation of root growth (increased microbial auxin production) in combination with NH4-induced rhizosphere acidification but not by BE-induced promotion of enzymatic nutrient turn-over in the rhizosphere.
Inoculation of plants with large consortia of PGPMs, instead of single strains with selected properties, may be a strategy to increase the flexibility of PGPM-host plant interactions in response to variable environmental conditions
NH4+ fertilization increased the auxin production potential
of rhizosphere bacteria in the ECAG2895 variants.
BE increased the shoot P content, correlated with increased total root length particularly in the ammonium-treated variants.
Klára Bradáčová1, Max Sittinger1, Markus Weinmann1, Günter Neumann1, Uwe Ludewig1,
Ellen Kandeler1, Nils Berger2
1Institute of Crop Science and Institute of Soil Science, University Hohenheim, 70593 Stuttgart, Germany
2EuroChem Agro, Reichskanzler-Müller Straße 23, 68165 Mannheim, Germany
Model for PGPM interactions in the rhizosphere
Lower rhizosphere acid phosphatase activity in ECAG2895 variants as compared with the unfertilized control.
No significant differences for alkaline phosphatase, glucosidase, xylosidase, cellulase and peptidase.
No BE
BE
Root length [cm]
P content in shoot tissue [mg P plant -1]
NO3- fertilisation
NH4+ fertilisation
0-Ctrl
0-Ctrl
NO3-
NH4+
Rhizosphere acid phosphatase activity
[nmol* g soil-1 h-1]
0-Ctrl
Nitrate fertilisation
Ammonium fertilisation
NO3-
NH4+
0-Ctrl
Rhizosphere- bacterial auxin production potential
IAA Production mg-1 bacterial biomass
[rel. values]
*
::ObservedscenarioPlant growthpromotionBetternutrientsupplyExpectedscenarioImprovedspatialP acquisitionRhizosphere acidificationP solubilizationH+H+H+Higher rootcolonizationbybacteriaIncreasedproductionofIAARoot elongationStimulation ofrootgrowthIncreasedC, N, P turnoverin rhizosphereImprovednutrientavailabilityModifiedfromRichardson, 2011
0-CtrlNitrate fertilisationAmmonium fertilisation
020406080100120140160180no BE BEIAA production[relative valuesmg microbialbiomass-1] Nitrate fertilisationAmmonium fertilisationabbbb
Slide Number 1Microbial Consortia Products (MCP)Introduction of Microbial Consortia Product (MCP)Slide Number 4Slide Number 5Slide Number 6Slide Number 7Slide Number 8Slide Number 9