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ASHISH DADHEECH ASHISH DADHEECH MSC NURSING IN PSYCHIATRIC TOPIC REGARDING RESEARCH

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Page 1: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

ASHISH DADHEECHASHISH DADHEECHMSC NURSING IN PSYCHIATRIC TOPIC REGARDING RESEARCH

Page 2: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

ANOVA ProceduresANOVA Procedures ANOVA (1 way)

◦ An extension of the 2 sample t-test used to determine if there are differences among > 2 group means

ANOVA with trend test◦ Test for polynomial trend in the group means

ANOVA (2 way)◦ Evaluate the combined effect of 2 experimental factors

ANOVA repeated measures◦ Extension of paired t-test for same or related subjects

over time or in differing circumstances ANCOVA

◦ 1 way ANOVA in which group means are adjusted by a covariate

Page 3: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

1 way ANOVA 1 way ANOVA AssumptionsAssumptionsIndependent SamplesNormality within each groupEqual variances

◦ Within group variances are the same for each of the groups

◦ Becomes less important if your sample sizes are similar among your groups

Ho: µ1= µ2= µ3=……… µk Ha: the population means of at least 2 groups

are different

Page 4: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

What is ANOVA?What is ANOVA? One-Way ANOVA allows us to compare the means of two or

more groups (the independent variable) on one dependent variable to determine if the group means differ significantly from one another.

In order to use ANOVA, we must have a categorical (or nominal) variable that has at least two independent groups (e.g. nationality, grade level) as the independent variable and a continuous variable (e.g., IQ score) as the dependent variable.

At this point, you may be thinking that ANOVA sounds very similar to what you learned about t tests. This is actually true if we are only comparing two groups. But when we’re looking at three or more groups, ANOVA is much more effective in determining significant group differences. The next slide explains why this is true.

Page 5: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Why use ANOVA vs a t-Why use ANOVA vs a t-testtestANOVA preserves the significance

level◦If you took 4 independent samples from

the same population and made all possible comparisons using t-tests (6 total comparisons) at .05 alpha level there is a 1-(0.95)^6= 0.26 probability that at least 1/6 of the comparisons will result in a significant difference > 25% chance we reject Ho when it is true

Page 6: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Homogeneity of VarianceHomogeneity of Variance It is important to determine

whether there are roughly an equal number of cases in each group and whether the amount of variance within each group is roughly equal• The ideal situation in ANOVA

is to have roughly equal sample sizes in each group and a roughly equal amount of variation (e.g., the standard deviation) in each group

• If the sample sizes and the standard deviations are quite different in the various groups, there is a problem

Group 1Group 1Group 2Group 2

Page 7: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

tt Tests vs. ANOVA Tests vs. ANOVA As you may recall, t tests allow us to decide whether the

observed difference between the means of two groups is large enough not to be due to chance (i.e., statistically significant).

However, each time we reach a conclusion about statistical significance with t tests, there is a slight chance that we may be wrong (i.e., make a Type I error—see Chapter 7). So the more t tests we run, the greater the chances become of deciding that a t test is significant (i.e., that the means being compared are really different) when it really is not.

This is why one-way ANOVA is important. ANOVA takes into account the number of groups being compared, and provides us with more certainty in concluding significance when we are looking at three or more groups. Rather than finding a simple difference between two means as in a t test, in ANOVA we are finding the average difference between means of multiple independent groups using the squared value of the difference between the means.

Page 8: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

How does ANOVA work?How does ANOVA work? The question that we can address using ANOVA is this: Is the average amount of difference, or variation, between the scores of members of

different samples large or small compared to the average amount of variation within each sample, otherwise known as random error?

To answer this question, we have to determine three things. First, we have to calculate the average amount of variation within each of our samples. This is called the mean square within (MSw) or the mean square error (MSe).

This is essentially the same as the standard error that we use in t tests. Second, we have to find the average amount of variation between the group means. This is called the mean square between (MSb).

This is essentially the same as the numerator in the independent samples t test. Third: Now that we’ve found these two statistics, we must find their ratio by dividing the mean square between by the mean square error. This ratio provides our F value.

This is our old formula of dividing the statistic of interest (i.e., the average difference between the group means) by the standard error. When we have our F value we can look at our family of F distributions to see if the differences between the groups are statistically significant

Page 9: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Calculating the SSe and the Calculating the SSe and the SSbSSb The sum of squares error (The sum of squares error (SSe)SSe) represents the sum of the squared deviations between represents the sum of the squared deviations between

individual scores and their respective group means on the dependent variable. individual scores and their respective group means on the dependent variable. To find the To find the SSeSSe we: we:

The SThe SSbSb represents the sum of the squared deviations between group means and the represents the sum of the squared deviations between group means and the grand mean (the mean of all individual scores in all the groups combined on the grand mean (the mean of all individual scores in all the groups combined on the dependent variable)dependent variable)

To find the To find the SSbSSb we: we: 1. Subtract the grand mean from the group mean: ( X – TX ); T indicates total, or the

mean for the total group. 2. Square each of these deviation scores: ( X – TX )2. 3. Multiply each squared deviation by the number of cases in the group: [n( X – TX )2].

Add these squared deviations from each group together: [n( X – TX )2].

1. Subtract the group mean from each individual score in each group: (X – X ). 2. Square each of these deviation scores: (X – X )2. 3. Add them all up for each group: (X – X )2. 4. Then add up all of the sums of squares for all of the groups:

(X1 – 1X )2 + (X2 – 2X )2 + … + (Xk – kX )2

•The only real differences between the formula for calculating the SSe and the SSb are: 1. In the SSe we subtract the group mean from the individual scores in each group, whereas in the SSb we subtract the grand mean from each group mean. 2. In the SSb we multiply each squared deviation by the number of cases in each group. We must do this to get an approximate deviation between the group mean and the grand mean for each case in every group.

F F = = MSb/MSeMSb/MSe

Page 10: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Finding The MSe and MSbFinding The MSe and MSb To find the MSe and the MSb, we have

to find the sum of squares error (SSe) and the sum squares between (SSb).

The SSe represents the sum of the squared deviations between individual scores and their respective group means on the dependent variable.

The SSb represents the sum of the squared deviations between group means and the grand mean (the mean of all individual scores in all the groups combined on the dependent variable represented by the symbol Xt ).

MSe = SSe / (N-K)Where: K=The number of groupsN=The number of cases in all the group combined.

MSb = SSb / (K-1)Where: K= The number of groups

Once we have calculated the SSb and the SSe, we can convert these numbers into average squared deviation scores (our MSb and MSe). To do this, we need to divide our SS scores by the appropriate degrees of freedom. Because we are looking at scores between groups, our df for MSb is K-1. And because we are looking at individual scores, our df for MSe is N-K.

Page 11: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Calculating an Calculating an F F -Value-Value Once we’ve found our MSe and MSb,

calculating our F Value is simple. We simply divide one value by the other.

F = MSb/MSe After an observed F value (Fo) is

determined, you need to check in Appendix C for to find the critical F value (Fc). Appendix C provides a chart that lists critical values for F associated with different alpha levels.

Using the two degrees of freedom you have already determined, you can see if your observed value (Fo) is larger than the critical value (Fc). If Fo is larger, than the value is statistically significant and you can conclude that the difference between group means is large enough to not be due to chance.

Page 12: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Example of Dividing the Variance between an Example of Dividing the Variance between an Individual Score and the Grand Mean into Individual Score and the Grand Mean into Within-Group and Between-Group Within-Group and Between-Group ComponentsComponents

Page 13: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

ExampleExample Suppose I want to know

whether certain species of animals differ in the number of tricks they are able to learn. I select a random sample of 15 tigers, 15 rabbits, and 15 pigeons for a total of 45 research participants.

After training and testing the animals, I collect the data presented in the chart to the right.

AnimalAnimal Average Number Average Number of Tricks Learnedof Tricks Learned

TigerTiger 12.312.3

RabbitRabbit 3.13.1

PigeonPigeon 9.79.7

SSe = 110.6 SSb = 37.3

Page 14: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Example (continued)Example (continued) Step 1: Degrees of Freedom: Now that you have your SSe and your SSb the next step is to determine your two df

values. Remember, our df for MSb is K-1 and our df for MSe is N-K: df for (MSb)= 3-1 = 2

df for (MSe) = 45-3 = 42 Step 2: Critical F-Value: Using these two df values, look in Appendix C to determine your critical F value.

Based on the F-Value equation, our numerator is 2 and our denominator is 42. With an alpha value of .05, our critical F value is 3.22. Therefore, if our observed F value is larger than 3.22 we can conclude there is a significant difference between our three groups

Step 3: Calcuating MSb and MSe: Using our formulas from the previous slide:MSe =SSe / (N-K) AND MSb = SSb / (K-1)

MSe = 110.6/42 = 2.63 MSb = 37.3/2 = 18.65

Step 4: Calculating an Observed F-Value: Finally, we can calculate our Fo using the formula Fo = MSb / MSe

Fo = 18.65 / 2.63 = 7.09

Page 15: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Interpreting Our ResultsInterpreting Our Results Our Fo of 7.09 is larger than our Fc of 3.22. Thus, we can

conclude that our results are statistically significanct and the difference between the number of tricks that tigers, rabbits and pigeons can learn is not due to chance.

However, we do not know where this difference exists. In other words, although we know there is a significant difference between the groups, we do not know which groups differ significantly from one another.

In order to answer this question we must conduct additional post-hoc tests. Specifically, we need to conduct a Tukey HSD post-hoc test to determine which group means are significantly different from each other.

Page 16: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Tukey HSDTukey HSD The Tukey test compares each group mean to every other group mean by

using the familiar formula described for t tests in Chapter 9. Specifically, it is the mean of one group minus the mean of a second group divided by the standard error, represented by the following formula.

xsXXHSDTukey 21

g

ex n

MSs

Where: ng = the number of cases in each group

The Tukey HSD allows us to compares groups of the same size. Just as with our t tests and F values, we must first determine a critical Tukey value to compare our observed Tukey values with. Using Appendix D, locate the number of groups we are comparing in the top row of the table, and then locate the degrees of freedom, error (dfe) in the left-hand column (The same dfe we used to find our F value).

Page 17: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Tukey HSD for our ExampleTukey HSD for our Example Now let’s use Tukey HSD to determine which animals differ significantly from each

other. First: Determine your critical Tukey Value using Appendix D. There are three groups,

and your dfe is still 42. Thus, your critical Tukey value is approximately 3.44. Now, calculate observed Tukey values to compare group means. Let’s look at Tigers

and Rabbits in detail. Then you can calculate the other two Tukeys on your own! xs

XXHSDTukey 21

g

ex n

MSs

Where: ng = the number of cases in each group

Step 1: Calculate your standard error. 2.63/15 = .18 √.175 = .42

Step 2: Subtract the mean number of tricks learned by rabbits by the mean number of tricks learned by tigers.

12.3-3.1 = 9.2Step 3: Divide this number by the standard error.

9.2 / .42 = 21.90

Step 4: Conclude that tigers learned significantly more tricks than rabbits.Repeat this process for the other group comparisons

Page 18: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Interpreting our Results. . . AgainInterpreting our Results. . . Again The table to the right lists the

observed Tukey values you should get for each animal pair.

Based on our critical Tukey value of 3.42, all of these observed values are statistically significant.

Now we can conclude that each of these groups differ significantly from one another. Another way to say this is that tigers learned significantly more tricks than pigeons and rabbits, and pigeons learned significantly more tricks than rabbits.

Animal Groups Animal Groups Being Being

ComparedCompared

Observed Observed TukeyTukeyValueValue

Tigers and Tigers and RabbitsRabbits 21.9021.90

Rabbits andRabbits andPigeonsPigeons 15.7115.71

Pigeons andPigeons andTigersTigers 6.196.19

Page 19: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

1 Way ANOVA with Trend Analysis1 Way ANOVA with Trend Analysis

Trend Analysis◦Groups have an order◦Ordinal categories or ordinal groups◦Testing hypothesis that means of

ordered groups change in a linear or higher order (cubic or quadratic)

Page 20: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Factorial or 2 way ANOVAEvaluate combined effect of 2

experimental variables (factors) on DV

Variables are categorical or nominal Are factors significant separately (main

effects) or in combination (interaction effects)Examples

How do age and gender affect the salaries of 10 year employees?

Investigators want to know the effects of dosage and gender on the effectiveness of a cholesterol-lowering drug

Page 21: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

2 way ANOVA hypothesisTest for interaction

Ho: there is no interaction effect Ha: there is an interaction effect

Test for Main Effects◦If there is not a significant

interaction Ho: population means are equal across

levels of Factor A, Factor B etc Ha: population means are not equal

across levels of Factor A, Factor B etchttp://www.uwsp.edu/PSYCH/stat/13/anova-2w.htm

Page 22: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Factorial ANOVA in DepthFactorial ANOVA in Depth When dividing up the variance

of a dependent variable, such as hours of television watched per week, into its component parts, there are a number of components that we can examine:• The main effects,• interaction effects,• simple effects, and• partial and controlled effects

Main Main effectseffectsInteraction

Interaction effectseffects

Simple effectsSimple effects

Partial and Partial and Controlled Controlled effectseffects

Page 23: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Main Effects and Controlled or Main Effects and Controlled or Partial EffectsPartial Effects

When looking at the main effects, it is possible to test whether there are significant differences between the groups of one independent variable on the dependent variable while controlling for, or partialing out, the effects of the other independent variable(s) on the dependent variable• Example - Boys watch significantly more television than girls. In addition,

suppose that children in the North watch, on average, more television than children in the South.

Now, suppose that, in my sample of children from the Northern region of the country, there are twice as many boys as girls, whereas in my sample from the South there are twice as many girls as boys. This could be a problem.

Once we remove that portion of the total variance that is explained by gender, we can test whether any additional part of the variance can be explained by knowing what region of the country children are from.

Page 24: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

When to use Factorial When to use Factorial ANOVA…ANOVA… Use when you have one continuous

(i.e., interval or ratio scaled) dependent variable and two or more categorical (i.e., nominally scaled) independent variables• Example - Do boys and girls

differ in the amount of television they watch per week, on average? Do children in different regions of the United States (i.e., East, West, North, and South) differ in their average amount of television watched per week? The average amount of television watched per week is the dependent variable, and gender and region of the country are the two independent variables.

Page 25: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Results of a Factorial Results of a Factorial ANOVAANOVA Two main effects, one for my

comparison of boys and girls and one for my comparison of children from different regions of the country• Definition: Main effects are

differences between the group means on the dependent variable for any independent variable in the ANOVA model.

An interaction effect, or simply an interaction• Definition: An interaction is

present when the differences between the group means on the dependent variable for one independent variable varies according to the level of a second independent variable.

• Interaction effects are also known as moderator effects

#x

#x

#x#x

Main Effect Main Effect

InteractionInteraction

Page 26: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

InteractionsInteractions Factorial ANOVA allows

researchers to test whether there are any statistical interactions present

The level of possible interactions increases as the number of independent variables increases• When there are two independent

variables in the analysis, there are two possible main effects and one possible two-way interaction effect

# variables# variables

# variables# variables

# of possible # of possible interactionsinteractions

# of possible # of possible interactionsinteractions

Page 27: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Example of an InteractionExample of an Interaction The relationship between

gender and amount of television watched depends on the region of the country• We appear to have a two-

way interaction here

Mean Amounts of Television Viewed by Gender and Region.Mean Amounts of Television Viewed by Gender and Region.

NorthNorth EastEast WestWest SouthSouth Overall Averages Overall Averages by Genderby Gender

GirlsGirls 2020 1515 1515 1010 1515

BoysBoys 2525 2020 2020 2525 22.522.5

Overall Overall AveragesAverages 22.522.5 17.517.5 17.517.5 17.517.5

• We can see is that We can see is that there is a consistent there is a consistent pattern for the pattern for the relationship between relationship between gender and amount of gender and amount of television viewed in television viewed in three of the regions three of the regions (North, East, and (North, East, and West), but in the fourth West), but in the fourth region (South) the region (South) the pattern changes pattern changes somewhatsomewhat

Page 28: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Graph of an InteractionGraph of an Interaction

0

5

10

15

20

25

30

North East West South

Region

Hou

rs p

er w

eek

of t.

v. w

atch

ed

BoysGirls

Page 29: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Example of a Factorial Example of a Factorial ANOVA ANOVA We conducted a study to see

whether high school boys and girls differed in their self-efficacy, whether students with relatively high GPAs differed from those with relatively low GPAs in their self-efficacy, and whether there was an interaction between gender and GPA on self-efficacy

Students’ self-efficacy was the dependent variable. Self-efficacy means how confident students are in their ability to do their schoolwork successfully

The results are presented on the next 2 slides

Page 30: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Example (continued)Example (continued)SPSS results for gender by GPA factorial ANOVA.SPSS results for gender by GPA factorial ANOVA.

GenderGender GPAGPA MeanMean Std. Dev.Std. Dev. NN

GirlGirl 1.001.00   3.66673.6667   .7758.7758 121121Girl 2.002.00   4.00504.0050   .7599.7599 133133Girl TotalTotal   3.84383.8438   .7845.7845 254254BoyBoy 1.001.00 3.93093.9309 .8494.8494 111111Boy 2.002.00 4.08094.0809 .8485.8485 103103Boy TotalTotal 4.00314.0031 .8503.8503 214214TotalTotal 1.001.00 3.79313.7931 .8208.8208 232232Total 2.002.00 4.03814.0381 .7989.7989 236236Total TotalTotal 3.91673.9167 .8182.8182 468468

These descriptive statistics reveal that high-achievers (group 2 in the GPA column) have higher self-efficacy than low achievers (group 1) and that the difference between high and low achievers appears to be larger among girls than among boys

Page 31: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Example (continued)Example (continued)ANOVA ResultsANOVA Results

SourceSource

Type III Type III Sum of Sum of SquaresSquares dfdf Mean SquareMean Square FF Sig.Sig.

Eta Eta SquareSquare

Corrected ModelCorrected Model   11.40211.402 33   3.8013.801   5.8545.854 .001.001 .036.036InterceptIntercept 7129.4357129.435 11 7129.4357129.435 10981.56610981.566 .000.000 .959.959GenderGender 3.3543.354 11 3.3543.354 5.1665.166 .023.023 .011.011GPAGPA 6.9126.912 11 6.9126.912 10.64610.646 .001.001 .022.022Gender * GPAGender * GPA 1.0281.028 11 1.0281.028 1.5841.584 .209.209 .003.003ErrorError 301.237301.237 464464 .649.649TotalTotal 7491.8897491.889 468468Corrected TotalCorrected Total 312.639312.639 467467

These ANOVA statistics reveal that there is a statistically significant main effect for gender, another significant main effect for GPA group, but no significant gender by GPA group interaction. Combined with the descriptive statistics on the previous slide we can conclude that boys have higher self-efficacy than girls, high GPA students have higher self-efficacy than low GPA students, and there is no interaction between gender and GPA on self-efficacy. Looking at the last column of this table we can also see that the effect sizes are quite small (eta squared = .011 for the gender effect and .022 for the GPA effect).

Page 32: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Example: Burger (1986)Example: Burger (1986) Examined the effects of choice and public versus

private evaluation on college students’ performance on an anagram-solving task

One dependent and two independent variables• Dependent variable: the number of anagrams

solved by participants in a 2-minute period• Independent variables: choice or no choice; public

or privateMean number of anagrams solved for four Mean number of anagrams solved for four treatment groups.treatment groups.

PublicPublic PrivatePrivate

ChoiceChoice No No ChoiceChoice ChoiceChoice No No

ChoiceChoice

Number of Number of anagrams solvedanagrams solved 19.5019.50 14.8614.86 14.9214.92 15.3615.36

Page 33: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Burger (1986) ConcludedBurger (1986) Concluded Found a main effect for choice, with students in the two choice groups

combined solving more anagrams, on average, than students in the two no-choice groups combined

Found a main effect for public over private performance Found an interaction between choice and public/private. Note the

difference between the public and private performance groups in the Choice condition.

0

5

10

15

20

25

Choice No Choice

Num

ber o

f ana

gram

s co

rrec

tly s

olve

d

PublicPrivate

Page 34: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Repeated-Measures ANOVA Repeated-Measures ANOVA versus Paired versus Paired t t TestTest Similar to a paired, or dependent samples t test,

repeated-measures ANOVA allows you to test whether there are significant differences between the scores of a single sample on a single variable measured at more than one time.

Unlike paired t tests, repeated-measures ANOVA lets you◦ Examine change on a variable measured across more

than two time points;◦ Include a covariate in the model;◦ Include categorical (i.e., between-subjects) independent

variables in the model;◦ Examine interactions between within-subject and

between-subject independent variables on the dependent variable

Page 35: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Different Types of Repeated Different Types of Repeated Measures ANOVAs (and when Measures ANOVAs (and when to use each type)to use each type)The most basic model

One sampleOne dependent variable

measured on an interval or ratio scale

The dependent variable is measured at least two different times Example: Measuring the

reaction time of a sample of people before they drink two beers and after they drink two beers

Time 1: Reaction time with no drinks

Time 2: Reaction time after two beers

Time, or trial

Page 36: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Different Types of Repeated Different Types of Repeated Measures ANOVAs (and Measures ANOVAs (and when to use each type)when to use each type)

Adding a between-subjects Adding a between-subjects independent variable to independent variable to the basic modelthe basic model

One sample with multiple One sample with multiple categories (e.g., boys and girls; categories (e.g., boys and girls; American, French, and Greek), or American, French, and Greek), or multiple samplesmultiple samples

One dependent variable One dependent variable measured on an interval or ratio measured on an interval or ratio scalescale

The dependent variable is The dependent variable is measured at least two different measured at least two different times. (Time, or trial, is the times. (Time, or trial, is the independent, within-subjects independent, within-subjects variable)variable)

Example: Measuring the Example: Measuring the reaction time of men and reaction time of men and women before they drink two women before they drink two beers and after they drink beers and after they drink two beerstwo beers

Time 1, Group 1: Reaction time of

men with no drinks

Time 2, Group 1: Reaction time

of menafter two beers

Time, or trial

Time 1, Group 2: Reaction time of

womenafter two beers

Time 2, Group 2: Reaction time

of womenafter two beers

Page 37: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Different Types of Repeated Different Types of Repeated Measures ANOVAs (and Measures ANOVAs (and when to use each type)when to use each type)

Adding a covariate to the Adding a covariate to the between-subjects between-subjects independent variable to the independent variable to the basic modelbasic model

One sample with multiple categories One sample with multiple categories (e.g., boys and girls; American, French, (e.g., boys and girls; American, French, and Greek), or multiple samplesand Greek), or multiple samples

One dependent variable measured on One dependent variable measured on an interval or ratio scalean interval or ratio scale

One covariate measured on an One covariate measured on an interval/ratio scale or dichotomouslyinterval/ratio scale or dichotomously

The dependent variable is measured at The dependent variable is measured at least two different times. (Time, or least two different times. (Time, or trial, is the independent, within-trial, is the independent, within-subjects variable)subjects variable)

Example: Measuring the reaction Example: Measuring the reaction time of men and women before time of men and women before they drink two beers and after they drink two beers and after they drink two beers, controlling they drink two beers, controlling for weight of the participantsfor weight of the participants

Time 1, Group 1: Reaction time of

men with no drinks

Time 2, Group 1: Reaction time

of menafter two beers

Time, or trial

Time 1, Group 2: Reaction time of

womenafter two beers

Time 2, Group 2: Reaction time

of womenafter two beers

Covariate

Page 38: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Types of Variance in Types of Variance in Repeated-Measures ANOVARepeated-Measures ANOVA Within-subject

◦ Variance in the dependent variable attributable to change, or difference, over time or across trials (e.g., changes in reaction time within the sample from the first test to the second test)

Between-subject◦ Variance in the dependent variable attributable to differences

between groups (e.g., men and women). Interaction

◦ Variance in the dependent across time, or trials, that differs by levels of the between-subjects variable (i.e., groups). For example, if women’s reaction time slows after drinking two beers but men’s reaction time does not.

Covariate◦ Variance in the dependent variable that is attributable to the

covariate. Covariates are included to see whether the independent variables are related to the dependent variable after controlling for the covariate. For example, does the reaction time of men and women change after drinking two beers once we control for the weight of the individuals?

Page 39: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Repeated Measures Repeated Measures SummarySummary Repeated-measures ANOVA should be used when you have

multiple measures of the same interval/ratio scaled dependent variable over multiple times or trials.

You can use it to partition the variance in the dependent variable into multiple components, including◦ Within-subjects (across time or trials)◦ Between-subjects (across multiple groups, or categories of an

independent variable)◦ Covariate◦ Interaction between the within-subjects and between-subjects

independent predictors As with all ANOVA procedures, repeated-measures ANOVA

assumes that the data are normally distributed and that there is homogeneity of variance across trials and groups.

Page 40: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

ANCOVA1 way ANOVA with a twist

Means not compared directly Means adjusted by a covariate Covariate is not controlled by researcher It is intrinsic to the subject observed

Page 41: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

Analysis of Analysis of CovarianceCovariance In ANOVA, the idea is to test whether there are

differences between groups on a dependent variable after controlling for the effects of a different variable, or set of variables

We have already discussed how we can examine the effects of one independent variable on the dependent variable after controlling for the effects of another independent variable

We can also control for the effects of a covariate. Unlike independent variables in ANOVA, covariates do not have to be categorical, or nominal, variables

Page 42: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

ANCOVA examplesA car dealership wants to know if

placing trucks, SUV’s, or sports cars in the display area impacts the number of customers who enter the showroom. Other factors may also impact the number such as the outside weather conditions. Thus outside weather becomes the covariate.

Page 43: Ashish Dadheech PRESENT TOPIC NURSING RESEARCH

AssumptionsCovariate must be quantitative an

linearly related to the outcome measure in each group

Regression line relating covariate to the response variable

Slopes of regression lines are equal

Ho: regression lines for each group are parallel Ha: at least two of the regression lines are not parallel Ho: all group means adjusted by the covariate are

equal Ha: at least 2 means adjusted by the covariate are not

equal