at the forefront of single cell genetic analysis
TRANSCRIPT
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AT THE FOREFRONT OFSINGLE CELL GENETIC ANALYSIS
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EUPLOIDY
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ANEUPLOIDY
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Aneuploidy rate in day 5 embryosHarton et al, 2013 Fert Steril Dec 100 (6): 1695-703
Live birth rateHFEA Fertility Treatment in 2012: trends and figures
<35 35-37 38-40 41-42 >42Maternal age
100%
80%
60%
40%
20%
0%
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PRE-IMPLANTATION GENETIC SCREENING
(PGS)
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Selecting only euploid embryos to transfer with PGS has been
demonstrated to:
• reduce the time to pregnancy (and IVF success);
• reduce the risk of miscarriage;
• overcome the adverse effect of maternal age on IVF success;
• allow the selection of only euploid embryos for freezing; and
• reduce the risk of multiple pregnancy from IVF by
supporting single embryo transfer
96 percent of
aneuploid embryos
fail to implant
Scott et al 2012 Fert Steril Apr 97 (4): 870-5
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EmbryoCellect™ is for research use only and is not for use in diagnostic procedures.
EmbryoCellectTM
Pre-implantation Genetic Screening Kit
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SAMPLE COLLECTION – EMBRYO BIOPSY
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EmbryoCellectTM
Pre-implantation Genetic Screening Kit
CELL LYSIS & AMPLIFICATION
15mins 2.5
hrs
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EmbryoCellectTM
Pre-implantation Genetic Screening KitLABELLING
TEST SAMPLE REFERENCE
45mins
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EmbryoCellectTM
Pre-implantation Genetic Screening Kit
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EmbryoCellectTM
Pre-implantation Genetic Screening KitHYBRIDISATION
TEST REFERENCE
3hrs -overnig
ht
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EmbryoCellectTM
Pre-implantation Genetic Screening KitRESULT: EUPLOID
TEST REFERENCE
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EmbryoCellectTM
Pre-implantation Genetic Screening KitRESULT: TRISOMY
TEST REFERENCE
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EmbryoCellectTM
Pre-implantation Genetic Screening KitRESULT: MONOSOMY
TEST REFERENCE
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TRISOMY
MONOSOMYRati
o t
est
:refe
ren
ce
Chromosome
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EmbryoCellect™Kit contents
Cell lysis buffer and enzyme for lysing the sample cells
Reference male gDNA
Whole Genome Amplification (WGA) reagents
Fluorescent labelling PCR reagents
Five patented EmbryoCellect™ microarray slides with four microarrays per slide
Software: An Excel macro that analyses the microarray scanner-generated data file The
EmbryoCellect™ kit contains
enough reagents to test 20 samples
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The test is simple and robust
The results are easy to interpret
The raw scanner data is available
for IVF labs to use for internal QC, if
required
The test has been validated for
accuracy
The test has been designed for labs to bring testing
in-house by providing the
flexibility to run small numbers of
samples
Why EmbryoCellect™?
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EmbryoCellectTM
Pre-implantation Genetic Screening KitLABORATORY SET UP OVERVIEWSPACE - Dedicated PCR-free laboratory & PCR
laboratory
STAFF – Experience in molecular biology / PCR
EQUIPMENT – Microarray scanner (€35k) and
other general molecular biology laboratory
equipment (~ € 35k)
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EmbryoCellectTM
Pre-implantation Genetic Screening KitVERSUS 24SURE
EmbryoCellect™ 24sure
Data obtained Whole chromosome aneuploidy
Whole chromosome aneuploidy
Unbalanced segmental errors >10Mb
Main equipment cost
Scanner =~ € 35k Scanner = ~ € 35k
Batch size 4 12-24
Interpretation Easy Moderate
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EmbryoCellectTM
Pre-implantation Genetic Screening KitVERSUS Next Generation Sequencing
EmbryoCellect™ NGS
Data obtained Whole chromosome aneuploidy
Whole chromosome aneuploidy
Unbalanced segmental errors >10Mb
Main equipment cost
Scanner = ~ € 35k MiSeq= ~ € 100k
Batch size 4 24
Interpretation Easy Complicated
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